##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanII BbvCI BclI BcnI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BsaBI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1286I BspDI BspEI BspMI BsrBI
BsrFI BssHII BssSI BstBI BstXI
BstYI CelII Cfr10I ClaI CpoI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FauI FbaI
FseI FspI HapII HpaI HpaII
KpnI KspI MamI MflI MluI
MluNI MroI MscI MspI NaeI
NciI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I Tth111I XcmI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 12 CGCG CG^CG
AcyI 31 GGCGCC G[GA]^CG[CT]C
AflIII 1127 ACATGT A^C[GA][CT]GT
AlwI 1195 GGATC GGATC(4/5)
BanI 31 GGCGCC G^G[CT][GA]CC
BbeI 31 GGCGCC GGCGC^C
BbrPI 678 CACGTG CAC^GTG
BbsI 975 GAAGAC GAAGAC(2/6)
BpuAI 975 GAAGAC GAAGAC(2/6)
BsaAI 678 CACGTG [CT]AC^GT[GA]
BsaHI 31 GGCGCC G[GA]^CG[CT]C
BsgI 454 CTGCAC GTGCAG(16/14)
BsmFI 381 GTCCC GGGAC(10/14)
BspHI 1535 TCATGA T^CATGA
BspLU11I 1127 ACATGT A^CATGT
Bst1107I 1520 GTATAC GTA^TAC
BstEII 1734 GGTGACC G^GT[ACGT]ACC
BstPI 1734 GGTGACC G^GT[ACGT]ACC
BstUI 12 CGCG CG^CG
BstZ17I 1520 GTATAC GTA^TAC
DraII 1141 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 2764 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 2807 CCTAATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1141 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1734 GGTGACC G^GT[ACGT]ACC
EcoT22I 1610 ATGCAT ATGCA^T
HaeII 31 GGCGCC [GA]GCGC^[CT]
Hin1I 31 GGCGCC G[GA]^CG[CT]C
KasI 31 GGCGCC G^GCGCC
MvnI 12 CGCG CG^CG
NarI 31 GGCGCC GG^CGCC
NcoI 1324 CCATGG C^CATGG
NdeI 4423 CATATG CA^TATG
NheI 3045 GCTAGC G^CTAGC
NsiI 1610 ATGCAT ATGCA^T
PmaCI 678 CACGTG CAC^GTG
PmlI 678 CACGTG CAC^GTG
PpuMI 1141 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 105 CAGCTG CAG^CTG
RcaI 1535 TCATGA T^CATGA
SexAI 1082 ACCTGGT A^CC[AT]GGT
SfaNI 743 GCATC GCATC(5/9)
SfoI 31 GGCGCC GGC^GCC
StuI 4632 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1520 GTATAC GT^[AC][GT]AC
2558 GTCTAC
AflII 2082 CTTAAG C^TTAAG
3325 CTTAAG
AlwNI 895 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
1808 CAGTTTCTG
AocI 525 CCTGAGG CC^T[ACGT]AGG
3079 CCTAAGG
Asp700I 2045 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2611 GAAACATTTC
BcgI 343 GCACAATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4027 GCAAATATTTCG
BfrI 2082 CTTAAG C^TTAAG
3325 CTTAAG
BsmI 1608 GAATGC GAATGC(1/-1)
2449 GCATTC
Bsu36I 525 CCTGAGG CC^T[ACGT]AGG
3079 CCTAAGG
CfoI 11 GCGC GCG^C
32 GCGC
DsaI 1324 CCATGG C^C[GA][CT]GG
2763 CCACGG
EarI 3932 CTCTTC CTCTTC(1/4)
963 GAAGAG
Eco81I 525 CCTGAGG CC^T[ACGT]AGG
3079 CCTAAGG
EcoRI 305 GAATTC G^AATTC
4108 GAATTC
HhaI 11 GCGC GCG^C
32 GCGC
HinP1I 11 GCGC G^CGC
32 GCGC
HincII 1170 GTTGAC GT[CT]^[GA]AC
2216 GTCAAC
HindII 1170 GTTGAC GT[CT]^[GA]AC
2216 GTCAAC
HindIII 1243 AAGCTT A^AGCTT
1570 AAGCTT
Ksp632I 3932 CTCTTC CTCTTC(1/4)
963 GAAGAG
MaeII 679 ACGT A^CGT
1168 ACGT
MspA1I 105 CAGCTG C[AC]G^C[GT]G
1191 CAGCGG
PflMI 279 CCAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
894 CCAGACACTGG
PstI 832 CTGCAG CTGCA^G
1097 CTGCAG
ScaI 2586 AGTACT AGT^ACT
4125 AGTACT
SmlI 2082 CTTAAG C^T[CT][GA]AG
3325 CTTAAG
SphI 491 GCATGC GCATG^C
1778 GCATGC
SwaI 3420 ATTTAAAT ATTT^AAAT
3573 ATTTAAAT
TaqI 158 TCGA T^CGA
1343 TCGA
TthHB8I 158 TCGA T^CGA
1343 TCGA
Van91I 279 CCAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
894 CCAGACACTGG
XmnI 2045 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2611 GAAACATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AseI AsnI AvaII BbvI BciVI
BfaI BsaJI BseRI BsiYI BslI
BsmAI Bsp1407I BsrDI BsrGI BsrI
BssKI BstAPI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI EcoRII
EcoT14I Fnu4HI FokI HaeIII HgaI
HinfI HphI ItaI MaeI MaeIII
MboI MboII MfeI MnlI MseI
MslI MunI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PshBI
RsaI Sau3AI Sau96I ScrFI SfcI
SspBI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XbaI