################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbvCI BclI BcnI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I BspDI BspEI BspMI BsrBI BsrFI BssHII BssSI BstBI BstXI BstYI CelII Cfr10I ClaI CpoI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FauI FbaI FseI FspI HapII HpaI HpaII KpnI KspI MamI MflI MluI MluNI MroI MscI MspI NaeI NciI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I Tth111I XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccII 12 CGCG CG^CG AcyI 31 GGCGCC G[GA]^CG[CT]C AflIII 1127 ACATGT A^C[GA][CT]GT AlwI 1195 GGATC GGATC(4/5) BanI 31 GGCGCC G^G[CT][GA]CC BbeI 31 GGCGCC GGCGC^C BbrPI 678 CACGTG CAC^GTG BbsI 975 GAAGAC GAAGAC(2/6) BpuAI 975 GAAGAC GAAGAC(2/6) BsaAI 678 CACGTG [CT]AC^GT[GA] BsaHI 31 GGCGCC G[GA]^CG[CT]C BsgI 454 CTGCAC GTGCAG(16/14) BsmFI 381 GTCCC GGGAC(10/14) BspHI 1535 TCATGA T^CATGA BspLU11I 1127 ACATGT A^CATGT Bst1107I 1520 GTATAC GTA^TAC BstEII 1734 GGTGACC G^GT[ACGT]ACC BstPI 1734 GGTGACC G^GT[ACGT]ACC BstUI 12 CGCG CG^CG BstZ17I 1520 GTATAC GTA^TAC DraII 1141 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 2764 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 2807 CCTAATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1141 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 1734 GGTGACC G^GT[ACGT]ACC EcoT22I 1610 ATGCAT ATGCA^T HaeII 31 GGCGCC [GA]GCGC^[CT] Hin1I 31 GGCGCC G[GA]^CG[CT]C KasI 31 GGCGCC G^GCGCC MvnI 12 CGCG CG^CG NarI 31 GGCGCC GG^CGCC NcoI 1324 CCATGG C^CATGG NdeI 4423 CATATG CA^TATG NheI 3045 GCTAGC G^CTAGC NsiI 1610 ATGCAT ATGCA^T PmaCI 678 CACGTG CAC^GTG PmlI 678 CACGTG CAC^GTG PpuMI 1141 AGGACCT [GA]G^G[AT]CC[CT] PvuII 105 CAGCTG CAG^CTG RcaI 1535 TCATGA T^CATGA SexAI 1082 ACCTGGT A^CC[AT]GGT SfaNI 743 GCATC GCATC(5/9) SfoI 31 GGCGCC GGC^GCC StuI 4632 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 1520 GTATAC GT^[AC][GT]AC 2558 GTCTAC AflII 2082 CTTAAG C^TTAAG 3325 CTTAAG AlwNI 895 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG 1808 CAGTTTCTG AocI 525 CCTGAGG CC^T[ACGT]AGG 3079 CCTAAGG Asp700I 2045 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2611 GAAACATTTC BcgI 343 GCACAATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4027 GCAAATATTTCG BfrI 2082 CTTAAG C^TTAAG 3325 CTTAAG BsmI 1608 GAATGC GAATGC(1/-1) 2449 GCATTC Bsu36I 525 CCTGAGG CC^T[ACGT]AGG 3079 CCTAAGG CfoI 11 GCGC GCG^C 32 GCGC DsaI 1324 CCATGG C^C[GA][CT]GG 2763 CCACGG EarI 3932 CTCTTC CTCTTC(1/4) 963 GAAGAG Eco81I 525 CCTGAGG CC^T[ACGT]AGG 3079 CCTAAGG EcoRI 305 GAATTC G^AATTC 4108 GAATTC HhaI 11 GCGC GCG^C 32 GCGC HinP1I 11 GCGC G^CGC 32 GCGC HincII 1170 GTTGAC GT[CT]^[GA]AC 2216 GTCAAC HindII 1170 GTTGAC GT[CT]^[GA]AC 2216 GTCAAC HindIII 1243 AAGCTT A^AGCTT 1570 AAGCTT Ksp632I 3932 CTCTTC CTCTTC(1/4) 963 GAAGAG MaeII 679 ACGT A^CGT 1168 ACGT MspA1I 105 CAGCTG C[AC]G^C[GT]G 1191 CAGCGG PflMI 279 CCAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 894 CCAGACACTGG PstI 832 CTGCAG CTGCA^G 1097 CTGCAG ScaI 2586 AGTACT AGT^ACT 4125 AGTACT SmlI 2082 CTTAAG C^T[CT][GA]AG 3325 CTTAAG SphI 491 GCATGC GCATG^C 1778 GCATGC SwaI 3420 ATTTAAAT ATTT^AAAT 3573 ATTTAAAT TaqI 158 TCGA T^CGA 1343 TCGA TthHB8I 158 TCGA T^CGA 1343 TCGA Van91I 279 CCAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 894 CCAGACACTGG XmnI 2045 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2611 GAAACATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AseI AsnI AvaII BbvI BciVI BfaI BsaJI BseRI BsiYI BslI BsmAI Bsp1407I BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraI EcoRII EcoT14I Fnu4HI FokI HaeIII HgaI HinfI HphI ItaI MaeI MaeIII MboI MboII MfeI MnlI MseI MslI MunI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PshBI RsaI Sau3AI Sau96I ScrFI SfcI SspBI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XbaI