##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanII       BbvCI       BclI        BcnI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BsaBI       BsaI        BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsoBI       
Bsp1286I    BspDI       BspEI       BspMI       BsrBI       
BsrFI       BssHII      BssSI       BstBI       BstXI       
BstYI       CelII       Cfr10I      ClaI        CpoI        
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRV       FauI        FbaI        
FseI        FspI        HapII       HpaI        HpaII       
KpnI        KspI        MamI        MflI        MluI        
MluNI       MroI        MscI        MspI        NaeI        
NciI        NgoMIV      NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    Tth111I     XcmI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccII          12  CGCG                 CG^CG
AcyI           31  GGCGCC               G[GA]^CG[CT]C
AflIII       1127  ACATGT               A^C[GA][CT]GT
AlwI         1195  GGATC                GGATC(4/5)
BanI           31  GGCGCC               G^G[CT][GA]CC
BbeI           31  GGCGCC               GGCGC^C
BbrPI         678  CACGTG               CAC^GTG
BbsI          975  GAAGAC               GAAGAC(2/6)
BpuAI         975  GAAGAC               GAAGAC(2/6)
BsaAI         678  CACGTG               [CT]AC^GT[GA]
BsaHI          31  GGCGCC               G[GA]^CG[CT]C
BsgI          454  CTGCAC               GTGCAG(16/14)
BsmFI         381  GTCCC                GGGAC(10/14)
BspHI        1535  TCATGA               T^CATGA
BspLU11I     1127  ACATGT               A^CATGT
Bst1107I     1520  GTATAC               GTA^TAC
BstEII       1734  GGTGACC              G^GT[ACGT]ACC
BstPI        1734  GGTGACC              G^GT[ACGT]ACC
BstUI          12  CGCG                 CG^CG
BstZ17I      1520  GTATAC               GTA^TAC
DraII        1141  AGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII       2764  CACGGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI        2807  CCTAATTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1141  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      1734  GGTGACC              G^GT[ACGT]ACC
EcoT22I      1610  ATGCAT               ATGCA^T
HaeII          31  GGCGCC               [GA]GCGC^[CT]
Hin1I          31  GGCGCC               G[GA]^CG[CT]C
KasI           31  GGCGCC               G^GCGCC
MvnI           12  CGCG                 CG^CG
NarI           31  GGCGCC               GG^CGCC
NcoI         1324  CCATGG               C^CATGG
NdeI         4423  CATATG               CA^TATG
NheI         3045  GCTAGC               G^CTAGC
NsiI         1610  ATGCAT               ATGCA^T
PmaCI         678  CACGTG               CAC^GTG
PmlI          678  CACGTG               CAC^GTG
PpuMI        1141  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII         105  CAGCTG               CAG^CTG
RcaI         1535  TCATGA               T^CATGA
SexAI        1082  ACCTGGT              A^CC[AT]GGT
SfaNI         743  GCATC                GCATC(5/9)
SfoI           31  GGCGCC               GGC^GCC
StuI         4632  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccI         1520  GTATAC               GT^[AC][GT]AC
             2558  GTCTAC              
AflII        2082  CTTAAG               C^TTAAG
             3325  CTTAAG              
AlwNI         895  CAGACACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1808  CAGTTTCTG           
AocI          525  CCTGAGG              CC^T[ACGT]AGG
             3079  CCTAAGG             
Asp700I      2045  GAATGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2611  GAAACATTTC          
BcgI          343  GCACAATCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             4027  GCAAATATTTCG        
BfrI         2082  CTTAAG               C^TTAAG
             3325  CTTAAG              
BsmI         1608  GAATGC               GAATGC(1/-1)
             2449  GCATTC              
Bsu36I        525  CCTGAGG              CC^T[ACGT]AGG
             3079  CCTAAGG             
CfoI           11  GCGC                 GCG^C
               32  GCGC                
DsaI         1324  CCATGG               C^C[GA][CT]GG
             2763  CCACGG              
EarI         3932  CTCTTC               CTCTTC(1/4)
              963  GAAGAG              
Eco81I        525  CCTGAGG              CC^T[ACGT]AGG
             3079  CCTAAGG             
EcoRI         305  GAATTC               G^AATTC
             4108  GAATTC              
HhaI           11  GCGC                 GCG^C
               32  GCGC                
HinP1I         11  GCGC                 G^CGC
               32  GCGC                
HincII       1170  GTTGAC               GT[CT]^[GA]AC
             2216  GTCAAC              
HindII       1170  GTTGAC               GT[CT]^[GA]AC
             2216  GTCAAC              
HindIII      1243  AAGCTT               A^AGCTT
             1570  AAGCTT              
Ksp632I      3932  CTCTTC               CTCTTC(1/4)
              963  GAAGAG              
MaeII         679  ACGT                 A^CGT
             1168  ACGT                
MspA1I        105  CAGCTG               C[AC]G^C[GT]G
             1191  CAGCGG              
PflMI         279  CCAAATCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              894  CCAGACACTGG         
PstI          832  CTGCAG               CTGCA^G
             1097  CTGCAG              
ScaI         2586  AGTACT               AGT^ACT
             4125  AGTACT              
SmlI         2082  CTTAAG               C^T[CT][GA]AG
             3325  CTTAAG              
SphI          491  GCATGC               GCATG^C
             1778  GCATGC              
SwaI         3420  ATTTAAAT             ATTT^AAAT
             3573  ATTTAAAT            
TaqI          158  TCGA                 T^CGA
             1343  TCGA                
TthHB8I       158  TCGA                 T^CGA
             1343  TCGA                
Van91I        279  CCAAATCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              894  CCAGACACTGG         
XmnI         2045  GAATGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2611  GAAACATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AseI        AsnI        AvaII       BbvI        BciVI       
BfaI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       Bsp1407I    BsrDI       BsrGI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HgaI        
HinfI       HphI        ItaI        MaeI        MaeIII      
MboI        MboII       MfeI        MnlI        MseI        
MslI        MunI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PshBI       
RsaI        Sau3AI      Sau96I      ScrFI       SfcI        
SspBI       SspI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XbaI