################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## AccIII AclI AflII AgeI AseI AsnI Asp700I BbeI BcgI BclI BfrI BsaBI BseAI Bsp1407I BspDI BspEI BsrBI BsrGI BstBI ClaI CpoI DraI DrdI EcoRV FbaI FseI KasI KspI MamI MfeI MluI MroI MunI NarI NheI NotI NruI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SfiI SfoI SfuI SgrAI SnaBI SspBI SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 3277 GACGTC GACGT^C Acc65I 3589 GGTACC G^GTACC AccI 742 GTATAC GT^[AC][GT]AC AcsI 3121 GAATTC [GA]^AATT[CT] AhdI 1627 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1014 CCTAAGG CC^T[ACGT]AGG ApoI 3121 GAATTC [GA]^AATT[CT] AscI 2450 GGCGCGCC GG^CGCGCC Asp718I 3589 GGTACC G^GTACC AspEI 1627 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 3534 CCTAGG C^CTAGG BbrPI 2392 CACGTG CAC^GTG BbvCI 1801 GCTGAGG CCTCAGC(-5/-2) BciVI 690 GGATAC GTATCC(6/5) BglI 1858 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2414 AGATCT A^GATCT BlnI 3534 CCTAGG C^CTAGG BsiWI 4373 CGTACG C^GTACG BspHI 3117 TCATGA T^CATGA BspLU11I 2333 ACATGT A^CATGT BssHII 2451 GCGCGC G^CGCGC BssSI 548 CTCGTG CACGAG(-5/-1) Bst1107I 742 GTATAC GTA^TAC BstAPI 1296 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 742 GTATAC GTA^TAC Bsu36I 1014 CCTAAGG CC^T[ACGT]AGG Eam1105I 1627 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1014 CCTAAGG CC^T[ACGT]AGG EcoRI 3121 GAATTC G^AATTC EcoT22I 3135 ATGCAT ATGCA^T HincII 2686 GTTAAC GT[CT]^[GA]AC HindII 2686 GTTAAC GT[CT]^[GA]AC HpaI 2686 GTTAAC GTT^AAC KpnI 3589 GGTACC GGTAC^C NdeI 2674 CATATG CA^TATG NsiI 3135 ATGCAT ATGCA^T PaeR7I 4384 CTCGAG C^TCGAG PmaCI 2392 CACGTG CAC^GTG PmlI 2392 CACGTG CAC^GTG PshAI 954 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 3117 TCATGA T^CATGA SbfI 3583 CCTGCAGG CCTGCA^GG ScaI 2726 AGTACT AGT^ACT SpeI 2380 ACTAGT A^CTAGT Sse8387I 3583 CCTGCAGG CCTGCA^GG Tsp509I 3122 AATT ^AATT XcmI 3263 CCATCACCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 4384 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AflIII 2333 ACATGT A^C[GA][CT]GT 4376 ACGTGT ApaI 1426 GGGCCC GGGCC^C 1546 GGGCCC AspI 1810 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3709 GACCTTGTC BamHI 360 GGATCC G^GATCC 3051 GGATCC BbsI 1635 GTCTTC GAAGAC(2/6) 2215 GTCTTC BlpI 1024 GCTGAGC GC^T[ACGT]AGC 4116 GCTGAGC Bpu1102I 1024 GCTGAGC GC^T[ACGT]AGC 4116 GCTGAGC BpuAI 1635 GTCTTC GAAGAC(2/6) 2215 GTCTTC BsaI 1499 GAGACC GGTCTC(1/5) 4191 GAGACC BsaWI 343 ACCGGA [AT]^CCGG[AT] 2112 ACCGGA BsmBI 2085 CGTCTC CGTCTC(1/5) 2251 GAGACG BstEII 339 GGTCACC G^GT[ACGT]ACC 2950 GGTGACC BstPI 339 GGTCACC G^GT[ACGT]ACC 2950 GGTGACC CelII 1024 GCTGAGC GC^T[ACGT]AGC 4116 GCTGAGC DraIII 468 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG 710 CACCAGGTG EagI 31 CGGCCG C^GGCCG 4355 CGGCCG EclXI 31 CGGCCG C^GGCCG 4355 CGGCCG Eco52I 31 CGGCCG C^GGCCG 4355 CGGCCG EcoO65I 339 GGTCACC G^GT[ACGT]ACC 2950 GGTGACC HindIII 1414 AAGCTT A^AGCTT 2050 AAGCTT NcoI 1175 CCATGG C^CATGG 1538 CCATGG PflFI 1810 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3709 GACCTTGTC SmlI 3007 CTCAAG C^T[CT][GA]AG 4384 CTCGAG StuI 2405 AGGCCT AGG^CCT 3042 AGGCCT Tth111I 1810 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3709 GACCTTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 128 enzymes) # ######################################### AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI Aor51HI ApaLI AspHI AvaI AvaII AviII BalI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmaI SphI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XhoII XmaCI XmaI