##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AccIII AclI AflII AgeI AseI
AsnI Asp700I BbeI BcgI BclI
BfrI BsaBI BseAI Bsp1407I BspDI
BspEI BsrBI BsrGI BstBI ClaI
CpoI DraI DrdI EcoRV FbaI
FseI KasI KspI MamI MfeI
MluI MroI MunI NarI NheI
NotI NruI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SfiI SfoI SfuI
SgrAI SnaBI SspBI SspI SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 3277 GACGTC GACGT^C
Acc65I 3589 GGTACC G^GTACC
AccI 742 GTATAC GT^[AC][GT]AC
AcsI 3121 GAATTC [GA]^AATT[CT]
AhdI 1627 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1014 CCTAAGG CC^T[ACGT]AGG
ApoI 3121 GAATTC [GA]^AATT[CT]
AscI 2450 GGCGCGCC GG^CGCGCC
Asp718I 3589 GGTACC G^GTACC
AspEI 1627 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3534 CCTAGG C^CTAGG
BbrPI 2392 CACGTG CAC^GTG
BbvCI 1801 GCTGAGG CCTCAGC(-5/-2)
BciVI 690 GGATAC GTATCC(6/5)
BglI 1858 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2414 AGATCT A^GATCT
BlnI 3534 CCTAGG C^CTAGG
BsiWI 4373 CGTACG C^GTACG
BspHI 3117 TCATGA T^CATGA
BspLU11I 2333 ACATGT A^CATGT
BssHII 2451 GCGCGC G^CGCGC
BssSI 548 CTCGTG CACGAG(-5/-1)
Bst1107I 742 GTATAC GTA^TAC
BstAPI 1296 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 742 GTATAC GTA^TAC
Bsu36I 1014 CCTAAGG CC^T[ACGT]AGG
Eam1105I 1627 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1014 CCTAAGG CC^T[ACGT]AGG
EcoRI 3121 GAATTC G^AATTC
EcoT22I 3135 ATGCAT ATGCA^T
HincII 2686 GTTAAC GT[CT]^[GA]AC
HindII 2686 GTTAAC GT[CT]^[GA]AC
HpaI 2686 GTTAAC GTT^AAC
KpnI 3589 GGTACC GGTAC^C
NdeI 2674 CATATG CA^TATG
NsiI 3135 ATGCAT ATGCA^T
PaeR7I 4384 CTCGAG C^TCGAG
PmaCI 2392 CACGTG CAC^GTG
PmlI 2392 CACGTG CAC^GTG
PshAI 954 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3117 TCATGA T^CATGA
SbfI 3583 CCTGCAGG CCTGCA^GG
ScaI 2726 AGTACT AGT^ACT
SpeI 2380 ACTAGT A^CTAGT
Sse8387I 3583 CCTGCAGG CCTGCA^GG
Tsp509I 3122 AATT ^AATT
XcmI 3263 CCATCACCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4384 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 2333 ACATGT A^C[GA][CT]GT
4376 ACGTGT
ApaI 1426 GGGCCC GGGCC^C
1546 GGGCCC
AspI 1810 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3709 GACCTTGTC
BamHI 360 GGATCC G^GATCC
3051 GGATCC
BbsI 1635 GTCTTC GAAGAC(2/6)
2215 GTCTTC
BlpI 1024 GCTGAGC GC^T[ACGT]AGC
4116 GCTGAGC
Bpu1102I 1024 GCTGAGC GC^T[ACGT]AGC
4116 GCTGAGC
BpuAI 1635 GTCTTC GAAGAC(2/6)
2215 GTCTTC
BsaI 1499 GAGACC GGTCTC(1/5)
4191 GAGACC
BsaWI 343 ACCGGA [AT]^CCGG[AT]
2112 ACCGGA
BsmBI 2085 CGTCTC CGTCTC(1/5)
2251 GAGACG
BstEII 339 GGTCACC G^GT[ACGT]ACC
2950 GGTGACC
BstPI 339 GGTCACC G^GT[ACGT]ACC
2950 GGTGACC
CelII 1024 GCTGAGC GC^T[ACGT]AGC
4116 GCTGAGC
DraIII 468 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
710 CACCAGGTG
EagI 31 CGGCCG C^GGCCG
4355 CGGCCG
EclXI 31 CGGCCG C^GGCCG
4355 CGGCCG
Eco52I 31 CGGCCG C^GGCCG
4355 CGGCCG
EcoO65I 339 GGTCACC G^GT[ACGT]ACC
2950 GGTGACC
HindIII 1414 AAGCTT A^AGCTT
2050 AAGCTT
NcoI 1175 CCATGG C^CATGG
1538 CCATGG
PflFI 1810 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3709 GACCTTGTC
SmlI 3007 CTCAAG C^T[CT][GA]AG
4384 CTCGAG
StuI 2405 AGGCCT AGG^CCT
3042 AGGCCT
Tth111I 1810 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3709 GACCTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI Aor51HI ApaLI
AspHI AvaI AvaII AviII BalI
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaAI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco47III EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmaI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XhoII XmaCI XmaI