##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccII AclI AflII AgeI
AhdI Alw44I ApaLI AscI AspEI
AspI AvrII BamHI BbrPI BfrI
BglI BlnI BsaAI BsiWI BsmBI
BspDI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I ClaI DraI
Eam1105I EcoO65I EcoRI EcoRV EcoT22I
FseI HindIII KspI MfeI MluI
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI Psp1406I SacII SalI
SbfI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 1643 GGTACC G^GTACC
AccI 2296 GTAGAC GT^[AC][GT]AC
AccIII 498 TCCGGA T^CCGGA
AflIII 1331 ACATGT A^C[GA][CT]GT
AlwNI 978 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3189 CCTAAGG CC^T[ACGT]AGG
Aor51HI 512 AGCGCT AGC^GCT
Asp700I 2380 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1643 GGTACC G^GTACC
BbeI 1366 GGCGCC GGCGC^C
BcgI 2885 GCAAGTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 42 GGATAC GTATCC(6/5)
BsaBI 1986 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 498 TCCGGA T^CCGGA
BsmI 25 GCATTC GAATGC(1/-1)
Bsp1407I 1478 TGTACA T^GTACA
BspEI 498 TCCGGA T^CCGGA
BspHI 2834 TCATGA T^CATGA
BspLU11I 1331 ACATGT A^CATGT
BspMI 1964 ACCTGC ACCTGC(4/8)
BsrDI 1745 GCAATG GCAATG(2/0)
BsrGI 1478 TGTACA T^GTACA
BssSI 178 CACGAG CACGAG(-5/-1)
Bsu36I 3189 CCTAAGG CC^T[ACGT]AGG
CpoI 4 CGGACCG CG^G[AT]CCG
DrdI 2591 GACGCAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 512 AGCGCT AGC^GCT
Eco81I 3189 CCTAAGG CC^T[ACGT]AGG
FauI 2793 CCCGC CCCGC(4/6)
HpaI 1819 GTTAAC GTT^AAC
KasI 1366 GGCGCC G^GCGCC
KpnI 1643 GGTACC GGTAC^C
MamI 1986 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 498 TCCGGA T^CCGGA
NarI 1366 GGCGCC GG^CGCC
PaeR7I 3006 CTCGAG C^TCGAG
PflMI 1182 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 270 GACCATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2552 CTGCAG CTGCA^G
PvuI 1709 CGATCG CGAT^CG
PvuII 683 CAGCTG CAG^CTG
RcaI 2834 TCATGA T^CATGA
RsrII 4 CGGACCG CG^G[AT]CCG
SapI 1252 GAAGAGC GCTCTTC(1/4)
ScaI 227 AGTACT AGT^ACT
SexAI 2990 ACCTGGT A^CC[AT]GGT
SfoI 1366 GGCGCC GGC^GCC
SspBI 1478 TGTACA T^GTACA
StuI 2717 AGGCCT AGG^CCT
Van91I 1182 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2973 TCTAGA T^CTAGA
XhoI 3006 CTCGAG C^TCGAG
XmnI 2380 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 568 GGCGTC G[GA]^CG[CT]C
1366 GGCGCC
ApaI 3001 GGGCCC GGGCC^C
3146 GGGCCC
AseI 897 ATTAAT AT^TAAT
1036 ATTAAT
AsnI 897 ATTAAT AT^TAAT
1036 ATTAAT
AviII 692 TGCGCA TGC^GCA
1856 TGCGCA
BbvCI 730 GCTGAGG CCTCAGC(-5/-2)
2125 GCTGAGG
BclI 761 TGATCA T^GATCA
2837 TGATCA
BfaI 2269 CTAG C^TAG
2974 CTAG
BlpI 20 GCTGAGC GC^T[ACGT]AGC
1524 GCTGAGC
Bpu1102I 20 GCTGAGC GC^T[ACGT]AGC
1524 GCTGAGC
BsaHI 568 GGCGTC G[GA]^CG[CT]C
1366 GGCGCC
BsaI 268 GAGACC GGTCTC(1/5)
2987 GAGACC
BsaWI 498 TCCGGA [AT]^CCGG[AT]
588 TCCGGT
BsrBI 754 GAGCGG CCGCTC(-3/-3)
1527 GAGCGG
BsrFI 818 ACCGGC [GA]^CCGG[CT]
1815 GCCGGT
BstXI 1889 CCACCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2439 CCACCGGGGTGG
CelII 20 GCTGAGC GC^T[ACGT]AGC
1524 GCTGAGC
Cfr10I 818 ACCGGC [GA]^CCGG[CT]
1815 GCCGGT
EagI 239 CGGCCG C^GGCCG
1515 CGGCCG
EclXI 239 CGGCCG C^GGCCG
1515 CGGCCG
Eco52I 239 CGGCCG C^GGCCG
1515 CGGCCG
FbaI 761 TGATCA T^GATCA
2837 TGATCA
FspI 692 TGCGCA TGC^GCA
1856 TGCGCA
HaeII 512 AGCGCT [GA]GCGC^[CT]
1366 GGCGCC
Hin1I 568 GGCGTC G[GA]^CG[CT]C
1366 GGCGCC
MaeI 2269 CTAG C^TAG
2974 CTAG
MspA1I 683 CAGCTG C[AC]G^C[GT]G
2794 CCGCTG
PshBI 897 ATTAAT AT^TAAT
1036 ATTAAT
SfcI 2252 CTACAG C^T[GA][CT]AG
2552 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BglII BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII