################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AccII AclI AflII AgeI AhdI Alw44I ApaLI AscI AspEI AspI AvrII BamHI BbrPI BfrI BglI BlnI BsaAI BsiWI BsmBI BspDI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I ClaI DraI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FseI HindIII KspI MfeI MluI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI Psp1406I SacII SalI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 1643 GGTACC G^GTACC AccI 2296 GTAGAC GT^[AC][GT]AC AccIII 498 TCCGGA T^CCGGA AflIII 1331 ACATGT A^C[GA][CT]GT AlwNI 978 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 3189 CCTAAGG CC^T[ACGT]AGG Aor51HI 512 AGCGCT AGC^GCT Asp700I 2380 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1643 GGTACC G^GTACC BbeI 1366 GGCGCC GGCGC^C BcgI 2885 GCAAGTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 42 GGATAC GTATCC(6/5) BsaBI 1986 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 498 TCCGGA T^CCGGA BsmI 25 GCATTC GAATGC(1/-1) Bsp1407I 1478 TGTACA T^GTACA BspEI 498 TCCGGA T^CCGGA BspHI 2834 TCATGA T^CATGA BspLU11I 1331 ACATGT A^CATGT BspMI 1964 ACCTGC ACCTGC(4/8) BsrDI 1745 GCAATG GCAATG(2/0) BsrGI 1478 TGTACA T^GTACA BssSI 178 CACGAG CACGAG(-5/-1) Bsu36I 3189 CCTAAGG CC^T[ACGT]AGG CpoI 4 CGGACCG CG^G[AT]CCG DrdI 2591 GACGCAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 512 AGCGCT AGC^GCT Eco81I 3189 CCTAAGG CC^T[ACGT]AGG FauI 2793 CCCGC CCCGC(4/6) HpaI 1819 GTTAAC GTT^AAC KasI 1366 GGCGCC G^GCGCC KpnI 1643 GGTACC GGTAC^C MamI 1986 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 498 TCCGGA T^CCGGA NarI 1366 GGCGCC GG^CGCC PaeR7I 3006 CTCGAG C^TCGAG PflMI 1182 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 270 GACCATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 2552 CTGCAG CTGCA^G PvuI 1709 CGATCG CGAT^CG PvuII 683 CAGCTG CAG^CTG RcaI 2834 TCATGA T^CATGA RsrII 4 CGGACCG CG^G[AT]CCG SapI 1252 GAAGAGC GCTCTTC(1/4) ScaI 227 AGTACT AGT^ACT SexAI 2990 ACCTGGT A^CC[AT]GGT SfoI 1366 GGCGCC GGC^GCC SspBI 1478 TGTACA T^GTACA StuI 2717 AGGCCT AGG^CCT Van91I 1182 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2973 TCTAGA T^CTAGA XhoI 3006 CTCGAG C^TCGAG XmnI 2380 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 568 GGCGTC G[GA]^CG[CT]C 1366 GGCGCC ApaI 3001 GGGCCC GGGCC^C 3146 GGGCCC AseI 897 ATTAAT AT^TAAT 1036 ATTAAT AsnI 897 ATTAAT AT^TAAT 1036 ATTAAT AviII 692 TGCGCA TGC^GCA 1856 TGCGCA BbvCI 730 GCTGAGG CCTCAGC(-5/-2) 2125 GCTGAGG BclI 761 TGATCA T^GATCA 2837 TGATCA BfaI 2269 CTAG C^TAG 2974 CTAG BlpI 20 GCTGAGC GC^T[ACGT]AGC 1524 GCTGAGC Bpu1102I 20 GCTGAGC GC^T[ACGT]AGC 1524 GCTGAGC BsaHI 568 GGCGTC G[GA]^CG[CT]C 1366 GGCGCC BsaI 268 GAGACC GGTCTC(1/5) 2987 GAGACC BsaWI 498 TCCGGA [AT]^CCGG[AT] 588 TCCGGT BsrBI 754 GAGCGG CCGCTC(-3/-3) 1527 GAGCGG BsrFI 818 ACCGGC [GA]^CCGG[CT] 1815 GCCGGT BstXI 1889 CCACCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2439 CCACCGGGGTGG CelII 20 GCTGAGC GC^T[ACGT]AGC 1524 GCTGAGC Cfr10I 818 ACCGGC [GA]^CCGG[CT] 1815 GCCGGT EagI 239 CGGCCG C^GGCCG 1515 CGGCCG EclXI 239 CGGCCG C^GGCCG 1515 CGGCCG Eco52I 239 CGGCCG C^GGCCG 1515 CGGCCG FbaI 761 TGATCA T^GATCA 2837 TGATCA FspI 692 TGCGCA TGC^GCA 1856 TGCGCA HaeII 512 AGCGCT [GA]GCGC^[CT] 1366 GGCGCC Hin1I 568 GGCGTC G[GA]^CG[CT]C 1366 GGCGCC MaeI 2269 CTAG C^TAG 2974 CTAG MspA1I 683 CAGCTG C[AC]G^C[GT]G 2794 CCGCTG PshBI 897 ATTAAT AT^TAAT 1036 ATTAAT SfcI 2252 CTACAG C^T[GA][CT]AG 2552 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BglII BmyI BpmI BpuAI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII