################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AhdI ApoI AscI AseI AsnI Asp700I Asp718I AspEI BamHI BbrPI BbvCI BciVI BclI BfrI BsaAI BsaBI BseAI BsiEI BsiWI BsmI Bsp1407I BspEI BsrGI BssHII BstBI BstEII BstPI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HpaI KpnI Ksp632I KspI MamI MluI MroI NdeI NheI NotI NsiI NspV PacI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AflIII 1922 ACATGT A^C[GA][CT]GT Alw44I 345 GTGCAC G^TGCAC AocI 537 CCTGAGG CC^T[ACGT]AGG Aor51HI 1309 AGCGCT AGC^GCT ApaLI 345 GTGCAC G^TGCAC AspI 612 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 1155 GGCGCC GGCGC^C BspDI 642 ATCGAT AT^CGAT BspHI 181 TCATGA T^CATGA BspLU11I 1922 ACATGT A^CATGT BssSI 132 CTCGTG CACGAG(-5/-1) Bst1107I 1347 GTATAC GTA^TAC BstZ17I 1347 GTATAC GTA^TAC Bsu36I 537 CCTGAGG CC^T[ACGT]AGG ClaI 642 ATCGAT AT^CGAT Eco47III 1309 AGCGCT AGC^GCT Eco81I 537 CCTGAGG CC^T[ACGT]AGG HindIII 1889 AAGCTT A^AGCTT KasI 1155 GGCGCC G^GCGCC MfeI 635 CAATTG C^AATTG MslI 2598 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 635 CAATTG C^AATTG NarI 1155 GGCGCC GG^CGCC PaeR7I 2868 CTCGAG C^TCGAG PflFI 612 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 181 TCATGA T^CATGA SacI 821 GAGCTC GAGCT^C ScaI 1276 AGTACT AGT^ACT SfoI 1155 GGCGCC GGC^GCC SphI 256 GCATGC GCATG^C TfiI 660 GATTC G^A[AT]TC Tsp509I 636 AATT ^AATT Tth111I 612 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1786 CCAGAGAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2868 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcyI 858 GACGCC G[GA]^CG[CT]C 1155 GGCGCC AlwI 143 GGATC GGATC(4/5) 2468 GGATC ApaI 1518 GGGCCC GGGCC^C 1576 GGGCCC AviII 79 TGCGCA TGC^GCA 502 TGCGCA AvrII 1585 CCTAGG C^CTAGG 1984 CCTAGG BcgI 208 GCATGGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2860 GCAGCTCTCTCG BglII 586 AGATCT A^GATCT 1300 AGATCT BlnI 1585 CCTAGG C^CTAGG 1984 CCTAGG BsaHI 858 GACGCC G[GA]^CG[CT]C 1155 GGCGCC BsmBI 1541 GAGACG CGTCTC(1/5) 1911 GAGACG BsrDI 596 CATTGC GCAATG(2/0) 2215 GCAATG BstAPI 2246 GCAAAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2755 GCAGTTGGTGC FspI 79 TGCGCA TGC^GCA 502 TGCGCA HgaI 858 GACGC GACGC(5/10) 1595 GACGC Hin1I 858 GACGCC G[GA]^CG[CT]C 1155 GGCGCC HincII 618 GTCAAC GT[CT]^[GA]AC 1851 GTCAAC HindII 618 GTCAAC GT[CT]^[GA]AC 1851 GTCAAC NaeI 1097 GCCGGC GCC^GGC 1359 GCCGGC NgoMIV 1097 GCCGGC G^CCGGC 1359 GCCGGC NruI 1337 TCGCGA TCG^CGA 1390 TCGCGA NspI 256 GCATGC [GA]CATG^[CT] 1922 ACATGT PpuMI 127 GGGACCT [GA]G^G[AT]CC[CT] 1402 AGGACCT SgrAI 373 CACCGGTG C[GA]^CCGG[CT]G 1474 CACCGGTG StuI 1860 AGGCCT AGG^CCT 2431 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccI AccII AciI AfaI AgeI AluI AlwNI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PinAI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XhoII XmaCI XmaI