##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AhdI        ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       BamHI       
BbrPI       BbvCI       BciVI       BclI        BfrI        
BsaAI       BsaBI       BseAI       BsiEI       BsiWI       
BsmI        Bsp1407I    BspEI       BsrGI       BssHII      
BstBI       BstEII      BstPI       CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco52I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HpaI        
KpnI        Ksp632I     KspI        MamI        MluI        
MroI        NdeI        NheI        NotI        NsiI        
NspV        PacI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfuI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AflIII       1922  ACATGT               A^C[GA][CT]GT
Alw44I        345  GTGCAC               G^TGCAC
AocI          537  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1309  AGCGCT               AGC^GCT
ApaLI         345  GTGCAC               G^TGCAC
AspI          612  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI         1155  GGCGCC               GGCGC^C
BspDI         642  ATCGAT               AT^CGAT
BspHI         181  TCATGA               T^CATGA
BspLU11I     1922  ACATGT               A^CATGT
BssSI         132  CTCGTG               CACGAG(-5/-1)
Bst1107I     1347  GTATAC               GTA^TAC
BstZ17I      1347  GTATAC               GTA^TAC
Bsu36I        537  CCTGAGG              CC^T[ACGT]AGG
ClaI          642  ATCGAT               AT^CGAT
Eco47III     1309  AGCGCT               AGC^GCT
Eco81I        537  CCTGAGG              CC^T[ACGT]AGG
HindIII      1889  AAGCTT               A^AGCTT
KasI         1155  GGCGCC               G^GCGCC
MfeI          635  CAATTG               C^AATTG
MslI         2598  CATGGAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          635  CAATTG               C^AATTG
NarI         1155  GGCGCC               GG^CGCC
PaeR7I       2868  CTCGAG               C^TCGAG
PflFI         612  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI          181  TCATGA               T^CATGA
SacI          821  GAGCTC               GAGCT^C
ScaI         1276  AGTACT               AGT^ACT
SfoI         1155  GGCGCC               GGC^GCC
SphI          256  GCATGC               GCATG^C
TfiI          660  GATTC                G^A[AT]TC
Tsp509I       636  AATT                 ^AATT
Tth111I       612  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1786  CCAGAGAGGGTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         2868  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcyI          858  GACGCC               G[GA]^CG[CT]C
             1155  GGCGCC              
AlwI          143  GGATC                GGATC(4/5)
             2468  GGATC               
ApaI         1518  GGGCCC               GGGCC^C
             1576  GGGCCC              
AviII          79  TGCGCA               TGC^GCA
              502  TGCGCA              
AvrII        1585  CCTAGG               C^CTAGG
             1984  CCTAGG              
BcgI          208  GCATGGTCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2860  GCAGCTCTCTCG        
BglII         586  AGATCT               A^GATCT
             1300  AGATCT              
BlnI         1585  CCTAGG               C^CTAGG
             1984  CCTAGG              
BsaHI         858  GACGCC               G[GA]^CG[CT]C
             1155  GGCGCC              
BsmBI        1541  GAGACG               CGTCTC(1/5)
             1911  GAGACG              
BsrDI         596  CATTGC               GCAATG(2/0)
             2215  GCAATG              
BstAPI       2246  GCAAAGGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2755  GCAGTTGGTGC         
FspI           79  TGCGCA               TGC^GCA
              502  TGCGCA              
HgaI          858  GACGC                GACGC(5/10)
             1595  GACGC               
Hin1I         858  GACGCC               G[GA]^CG[CT]C
             1155  GGCGCC              
HincII        618  GTCAAC               GT[CT]^[GA]AC
             1851  GTCAAC              
HindII        618  GTCAAC               GT[CT]^[GA]AC
             1851  GTCAAC              
NaeI         1097  GCCGGC               GCC^GGC
             1359  GCCGGC              
NgoMIV       1097  GCCGGC               G^CCGGC
             1359  GCCGGC              
NruI         1337  TCGCGA               TCG^CGA
             1390  TCGCGA              
NspI          256  GCATGC               [GA]CATG^[CT]
             1922  ACATGT              
PpuMI         127  GGGACCT              [GA]G^G[AT]CC[CT]
             1402  AGGACCT             
SgrAI         373  CACCGGTG             C[GA]^CCGG[CT]G
             1474  CACCGGTG            
StuI         1860  AGGCCT               AGG^CCT
             2431  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  114 enzymes) #
#########################################

AccI        AccII       AciI        AfaI        AgeI        
AluI        AlwNI       AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
PflMI       PinAI       PleI        PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        SmlI        StyI        TaqI        
Tru9I       TseI        Tsp45I      TspRI       TthHB8I     
Van91I      XhoII       XmaCI       XmaI