##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AhdI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI BamHI
BbrPI BbvCI BciVI BclI BfrI
BsaAI BsaBI BseAI BsiEI BsiWI
BsmI Bsp1407I BspEI BsrGI BssHII
BstBI BstEII BstPI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HpaI
KpnI Ksp632I KspI MamI MluI
MroI NdeI NheI NotI NsiI
NspV PacI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfuI SnaBI SpeI Sse8387I
SspBI SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 1922 ACATGT A^C[GA][CT]GT
Alw44I 345 GTGCAC G^TGCAC
AocI 537 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1309 AGCGCT AGC^GCT
ApaLI 345 GTGCAC G^TGCAC
AspI 612 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1155 GGCGCC GGCGC^C
BspDI 642 ATCGAT AT^CGAT
BspHI 181 TCATGA T^CATGA
BspLU11I 1922 ACATGT A^CATGT
BssSI 132 CTCGTG CACGAG(-5/-1)
Bst1107I 1347 GTATAC GTA^TAC
BstZ17I 1347 GTATAC GTA^TAC
Bsu36I 537 CCTGAGG CC^T[ACGT]AGG
ClaI 642 ATCGAT AT^CGAT
Eco47III 1309 AGCGCT AGC^GCT
Eco81I 537 CCTGAGG CC^T[ACGT]AGG
HindIII 1889 AAGCTT A^AGCTT
KasI 1155 GGCGCC G^GCGCC
MfeI 635 CAATTG C^AATTG
MslI 2598 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 635 CAATTG C^AATTG
NarI 1155 GGCGCC GG^CGCC
PaeR7I 2868 CTCGAG C^TCGAG
PflFI 612 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 181 TCATGA T^CATGA
SacI 821 GAGCTC GAGCT^C
ScaI 1276 AGTACT AGT^ACT
SfoI 1155 GGCGCC GGC^GCC
SphI 256 GCATGC GCATG^C
TfiI 660 GATTC G^A[AT]TC
Tsp509I 636 AATT ^AATT
Tth111I 612 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1786 CCAGAGAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2868 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 858 GACGCC G[GA]^CG[CT]C
1155 GGCGCC
AlwI 143 GGATC GGATC(4/5)
2468 GGATC
ApaI 1518 GGGCCC GGGCC^C
1576 GGGCCC
AviII 79 TGCGCA TGC^GCA
502 TGCGCA
AvrII 1585 CCTAGG C^CTAGG
1984 CCTAGG
BcgI 208 GCATGGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2860 GCAGCTCTCTCG
BglII 586 AGATCT A^GATCT
1300 AGATCT
BlnI 1585 CCTAGG C^CTAGG
1984 CCTAGG
BsaHI 858 GACGCC G[GA]^CG[CT]C
1155 GGCGCC
BsmBI 1541 GAGACG CGTCTC(1/5)
1911 GAGACG
BsrDI 596 CATTGC GCAATG(2/0)
2215 GCAATG
BstAPI 2246 GCAAAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2755 GCAGTTGGTGC
FspI 79 TGCGCA TGC^GCA
502 TGCGCA
HgaI 858 GACGC GACGC(5/10)
1595 GACGC
Hin1I 858 GACGCC G[GA]^CG[CT]C
1155 GGCGCC
HincII 618 GTCAAC GT[CT]^[GA]AC
1851 GTCAAC
HindII 618 GTCAAC GT[CT]^[GA]AC
1851 GTCAAC
NaeI 1097 GCCGGC GCC^GGC
1359 GCCGGC
NgoMIV 1097 GCCGGC G^CCGGC
1359 GCCGGC
NruI 1337 TCGCGA TCG^CGA
1390 TCGCGA
NspI 256 GCATGC [GA]CATG^[CT]
1922 ACATGT
PpuMI 127 GGGACCT [GA]G^G[AT]CC[CT]
1402 AGGACCT
SgrAI 373 CACCGGTG C[GA]^CCGG[CT]G
1474 CACCGGTG
StuI 1860 AGGCCT AGG^CCT
2431 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AccII AciI AfaI AgeI
AluI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PinAI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XhoII XmaCI XmaI