##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccIII AclI AhdI
Alw44I ApaI ApaLI AscI Asp718I
AspEI AvrII BbrPI BcgI BciVI
BlnI BsaBI BseAI BsiWI BsmBI
BsmI BspDI BspEI BstBI BstEII
BstPI ClaI CpoI DraIII Eam1105I
EcoO65I EcoRI EcoRV FseI HindIII
KpnI MamI MfeI MluI MroI
MunI NotI NspV PacI PaeR7I
PmaCI PmlI PshAI Psp1406I PvuI
RsrII SalI SbfI ScaI SexAI
SfiI SfuI SmaI SnaBI SpeI
SphI Sse8387I SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcyI 52 GGCGCC G[GA]^CG[CT]C
AflII 1200 CTTAAG C^TTAAG
AgeI 868 ACCGGT A^CCGGT
AviII 3732 TGCGCA TGC^GCA
BamHI 2638 GGATCC G^GATCC
BbeI 52 GGCGCC GGCGC^C
BclI 1380 TGATCA T^GATCA
BfrI 1200 CTTAAG C^TTAAG
BsaAI 3630 CACGTA [CT]AC^GT[GA]
BsaHI 52 GGCGCC G[GA]^CG[CT]C
BsaI 2472 GAGACC GGTCTC(1/5)
BsaWI 868 ACCGGT [AT]^CCGG[AT]
Bsp1407I 1751 TGTACA T^GTACA
BspHI 1984 TCATGA T^CATGA
BsrDI 3219 CATTGC GCAATG(2/0)
BsrGI 1751 TGTACA T^GTACA
BssSI 325 CACGAG CACGAG(-5/-1)
Bst1107I 3903 GTATAC GTA^TAC
BstZ17I 3903 GTATAC GTA^TAC
DrdI 4224 GACATTCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1380 TGATCA T^GATCA
FspI 3732 TGCGCA TGC^GCA
Hin1I 52 GGCGCC G[GA]^CG[CT]C
HincII 3360 GTTAAC GT[CT]^[GA]AC
HindII 3360 GTTAAC GT[CT]^[GA]AC
HpaI 3360 GTTAAC GTT^AAC
KasI 52 GGCGCC G^GCGCC
NaeI 1413 GCCGGC GCC^GGC
NarI 52 GGCGCC GG^CGCC
NcoI 2831 CCATGG C^CATGG
NgoMIV 1413 GCCGGC G^CCGGC
NheI 1409 GCTAGC G^CTAGC
NruI 124 TCGCGA TCG^CGA
PinAI 868 ACCGGT A^CCGGT
PmeI 3081 GTTTAAAC GTTT^AAAC
RcaI 1984 TCATGA T^CATGA
SapI 3106 GCTCTTC GCTCTTC(1/4)
SfoI 52 GGCGCC GGC^GCC
SgrAI 867 CACCGGTG C[GA]^CCGG[CT]G
SspBI 1751 TGTACA T^GTACA
SspI 4642 AATATT AAT^ATT
StuI 2531 AGGCCT AGG^CCT
XcmI 2828 CCACCATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 3624 GTAGAC GT^[AC][GT]AC
3903 GTATAC
AocI 1626 CCTCAGG CC^T[ACGT]AGG
4266 CCTTAGG
Aor51HI 98 AGCGCT AGC^GCT
1207 AGCGCT
AseI 1535 ATTAAT AT^TAAT
2256 ATTAAT
AsnI 1535 ATTAAT AT^TAAT
2256 ATTAAT
Asp700I 2393 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2892 GAACATATTC
AspI 1968 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3682 GACACTGTC
BalI 1676 TGGCCA TGG^CCA
2621 TGGCCA
BbsI 1980 GTCTTC GAAGAC(2/6)
2903 GTCTTC
BbvCI 2981 CCTCAGC CCTCAGC(-5/-2)
1225 GCTGAGG
BglI 457 GCCTCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1349 GCCTTTAAGGC
BlpI 777 GCTCAGC GC^T[ACGT]AGC
2350 GCTTAGC
Bpu1102I 777 GCTCAGC GC^T[ACGT]AGC
2350 GCTTAGC
BpuAI 1980 GTCTTC GAAGAC(2/6)
2903 GTCTTC
BsgI 2969 CTGCAC GTGCAG(16/14)
3504 GTGCAG
BspMI 3471 ACCTGC ACCTGC(4/8)
1121 GCAGGT
BssHII 46 GCGCGC G^CGCGC
318 GCGCGC
BstAPI 74 GCAGAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3870 GCAATATATGC
BstXI 2322 CCAGAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2831 CCATGGCTCTGG
Bsu36I 1626 CCTCAGG CC^T[ACGT]AGG
4266 CCTTAGG
CelII 777 GCTCAGC GC^T[ACGT]AGC
2350 GCTTAGC
DraI 111 TTTAAA TTT^AAA
3082 TTTAAA
Eco47III 98 AGCGCT AGC^GCT
1207 AGCGCT
Eco81I 1626 CCTCAGG CC^T[ACGT]AGG
4266 CCTTAGG
MluNI 1676 TGGCCA TGG^CCA
2621 TGGCCA
MscI 1676 TGGCCA TGG^CCA
2621 TGGCCA
PflFI 1968 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3682 GACACTGTC
PshBI 1535 ATTAAT AT^TAAT
2256 ATTAAT
PvuII 760 CAGCTG CAG^CTG
1418 CAGCTG
SacI 1264 GAGCTC GAGCT^C
2325 GAGCTC
TaqI 530 TCGA T^CGA
1833 TCGA
Tth111I 1968 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3682 GACACTGTC
TthHB8I 530 TCGA T^CGA
1833 TCGA
XmnI 2393 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2892 GAACATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BglII BmyI BpmI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspLU11I BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoNI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PpuMI PstI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII