################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII Acc65I AccIII AclI AhdI Alw44I ApaI ApaLI AscI Asp718I AspEI AvrII BbrPI BcgI BciVI BlnI BsaBI BseAI BsiWI BsmBI BsmI BspDI BspEI BstBI BstEII BstPI ClaI CpoI DraIII Eam1105I EcoO65I EcoRI EcoRV FseI HindIII KpnI MamI MfeI MluI MroI MunI NotI NspV PacI PaeR7I PmaCI PmlI PshAI Psp1406I PvuI RsrII SalI SbfI ScaI SexAI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcyI 52 GGCGCC G[GA]^CG[CT]C AflII 1200 CTTAAG C^TTAAG AgeI 868 ACCGGT A^CCGGT AviII 3732 TGCGCA TGC^GCA BamHI 2638 GGATCC G^GATCC BbeI 52 GGCGCC GGCGC^C BclI 1380 TGATCA T^GATCA BfrI 1200 CTTAAG C^TTAAG BsaAI 3630 CACGTA [CT]AC^GT[GA] BsaHI 52 GGCGCC G[GA]^CG[CT]C BsaI 2472 GAGACC GGTCTC(1/5) BsaWI 868 ACCGGT [AT]^CCGG[AT] Bsp1407I 1751 TGTACA T^GTACA BspHI 1984 TCATGA T^CATGA BsrDI 3219 CATTGC GCAATG(2/0) BsrGI 1751 TGTACA T^GTACA BssSI 325 CACGAG CACGAG(-5/-1) Bst1107I 3903 GTATAC GTA^TAC BstZ17I 3903 GTATAC GTA^TAC DrdI 4224 GACATTCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 1380 TGATCA T^GATCA FspI 3732 TGCGCA TGC^GCA Hin1I 52 GGCGCC G[GA]^CG[CT]C HincII 3360 GTTAAC GT[CT]^[GA]AC HindII 3360 GTTAAC GT[CT]^[GA]AC HpaI 3360 GTTAAC GTT^AAC KasI 52 GGCGCC G^GCGCC NaeI 1413 GCCGGC GCC^GGC NarI 52 GGCGCC GG^CGCC NcoI 2831 CCATGG C^CATGG NgoMIV 1413 GCCGGC G^CCGGC NheI 1409 GCTAGC G^CTAGC NruI 124 TCGCGA TCG^CGA PinAI 868 ACCGGT A^CCGGT PmeI 3081 GTTTAAAC GTTT^AAAC RcaI 1984 TCATGA T^CATGA SapI 3106 GCTCTTC GCTCTTC(1/4) SfoI 52 GGCGCC GGC^GCC SgrAI 867 CACCGGTG C[GA]^CCGG[CT]G SspBI 1751 TGTACA T^GTACA SspI 4642 AATATT AAT^ATT StuI 2531 AGGCCT AGG^CCT XcmI 2828 CCACCATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccI 3624 GTAGAC GT^[AC][GT]AC 3903 GTATAC AocI 1626 CCTCAGG CC^T[ACGT]AGG 4266 CCTTAGG Aor51HI 98 AGCGCT AGC^GCT 1207 AGCGCT AseI 1535 ATTAAT AT^TAAT 2256 ATTAAT AsnI 1535 ATTAAT AT^TAAT 2256 ATTAAT Asp700I 2393 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2892 GAACATATTC AspI 1968 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3682 GACACTGTC BalI 1676 TGGCCA TGG^CCA 2621 TGGCCA BbsI 1980 GTCTTC GAAGAC(2/6) 2903 GTCTTC BbvCI 2981 CCTCAGC CCTCAGC(-5/-2) 1225 GCTGAGG BglI 457 GCCTCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1349 GCCTTTAAGGC BlpI 777 GCTCAGC GC^T[ACGT]AGC 2350 GCTTAGC Bpu1102I 777 GCTCAGC GC^T[ACGT]AGC 2350 GCTTAGC BpuAI 1980 GTCTTC GAAGAC(2/6) 2903 GTCTTC BsgI 2969 CTGCAC GTGCAG(16/14) 3504 GTGCAG BspMI 3471 ACCTGC ACCTGC(4/8) 1121 GCAGGT BssHII 46 GCGCGC G^CGCGC 318 GCGCGC BstAPI 74 GCAGAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3870 GCAATATATGC BstXI 2322 CCAGAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2831 CCATGGCTCTGG Bsu36I 1626 CCTCAGG CC^T[ACGT]AGG 4266 CCTTAGG CelII 777 GCTCAGC GC^T[ACGT]AGC 2350 GCTTAGC DraI 111 TTTAAA TTT^AAA 3082 TTTAAA Eco47III 98 AGCGCT AGC^GCT 1207 AGCGCT Eco81I 1626 CCTCAGG CC^T[ACGT]AGG 4266 CCTTAGG MluNI 1676 TGGCCA TGG^CCA 2621 TGGCCA MscI 1676 TGGCCA TGG^CCA 2621 TGGCCA PflFI 1968 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3682 GACACTGTC PshBI 1535 ATTAAT AT^TAAT 2256 ATTAAT PvuII 760 CAGCTG CAG^CTG 1418 CAGCTG SacI 1264 GAGCTC GAGCT^C 2325 GAGCTC TaqI 530 TCGA T^CGA 1833 TCGA Tth111I 1968 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3682 GACACTGTC TthHB8I 530 TCGA T^CGA 1833 TCGA XmnI 2393 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2892 GAACATATTC ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BglII BmyI BpmI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII