##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccI AclI AflII AflIII
AgeI AhdI Aor51HI AscI AspEI
AspI AviII BamHI BbvCI BcgI
BfrI BglI BlpI Bpu1102I BsiWI
BspDI BspLU11I BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraIII DrdI
Eam1105I Eco47III EcoO65I EcoRI FseI
FspI HincII HindII HpaI MfeI
MluI MunI NcoI NheI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI Psp1406I PvuI RsrII
SacI SalI SbfI SfiI SfuI
SgrAI SpeI Sse8387I StuI SwaI
Tth111I Van91I XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 1962 GGTACC G^GTACC
AccIII 320 TCCGGA T^CCGGA
Alw44I 3531 GTGCAC G^TGCAC
ApaI 334 GGGCCC GGGCC^C
ApaLI 3531 GTGCAC G^TGCAC
AseI 1116 ATTAAT AT^TAAT
AsnI 1116 ATTAAT AT^TAAT
Asp718I 1962 GGTACC G^GTACC
AvrII 1741 CCTAGG C^CTAGG
BbeI 210 GGCGCC GGCGC^C
BbrPI 1299 CACGTG CAC^GTG
BciVI 617 GGATAC GTATCC(6/5)
BglII 1259 AGATCT A^GATCT
BlnI 1741 CCTAGG C^CTAGG
BsaBI 3007 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 320 TCCGGA T^CCGGA
BseRI 738 CTCCTC GAGGAG(10/8)
BsiEI 175 CGGCCG CG[GA][CT]^CG
BsmBI 627 GAGACG CGTCTC(1/5)
Bsp1407I 3910 TGTACA T^GTACA
BspEI 320 TCCGGA T^CCGGA
BspHI 3539 TCATGA T^CATGA
BsrBI 32 GAGCGG CCGCTC(-3/-3)
BsrDI 3413 GCAATG GCAATG(2/0)
BsrFI 192 GCCGGC [GA]^CCGG[CT]
BsrGI 3910 TGTACA T^GTACA
Cfr10I 192 GCCGGC [GA]^CCGG[CT]
DraI 2310 TTTAAA TTT^AAA
EagI 175 CGGCCG C^GGCCG
EclXI 175 CGGCCG C^GGCCG
Eco52I 175 CGGCCG C^GGCCG
EcoRV 3845 GATATC GAT^ATC
HindIII 772 AAGCTT A^AGCTT
KasI 210 GGCGCC G^GCGCC
KpnI 1962 GGTACC GGTAC^C
MamI 3007 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 320 TCCGGA T^CCGGA
NaeI 192 GCCGGC GCC^GGC
NarI 210 GGCGCC GG^CGCC
NdeI 2057 CATATG CA^TATG
NgoMIV 192 GCCGGC G^CCGGC
NotI 174 GCGGCCGC GC^GGCCGC
PmaCI 1299 CACGTG CAC^GTG
PmlI 1299 CACGTG CAC^GTG
PshBI 1116 ATTAAT AT^TAAT
RcaI 3539 TCATGA T^CATGA
SapI 1516 GCTCTTC GCTCTTC(1/4)
ScaI 1147 AGTACT AGT^ACT
SexAI 2951 ACCTGGT A^CC[AT]GGT
SfoI 210 GGCGCC GGC^GCC
SnaBI 3345 TACGTA TAC^GTA
SspBI 3910 TGTACA T^GTACA
TaqI 50 TCGA T^CGA
TthHB8I 50 TCGA T^CGA
XbaI 2757 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 120 GACGCC G[GA]^CG[CT]C
210 GGCGCC
AlwNI 1495 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
2747 CAGAACCTG
AocI 486 CCTCAGG CC^T[ACGT]AGG
2536 CCTAAGG
AspHI 933 GTGCTC G[AT]GC[AT]^C
3531 GTGCAC
BalI 701 TGGCCA TGG^CCA
1589 TGGCCA
BclI 1156 TGATCA T^GATCA
3542 TGATCA
BsaAI 1299 CACGTG [CT]AC^GT[GA]
3345 TACGTA
BsaHI 120 GACGCC G[GA]^CG[CT]C
210 GGCGCC
BsaWI 320 TCCGGA [AT]^CCGG[AT]
963 TCCGGT
BsiHKAI 933 GTGCTC G[AT]GC[AT]^C
3531 GTGCAC
BsmI 656 GAATGC GAATGC(1/-1)
751 GCATTC
BspMI 1226 ACCTGC ACCTGC(4/8)
182 GCAGGT
BstYI 1259 AGATCT [GA]^GATC[CT]
3494 GGATCT
Bsu36I 486 CCTCAGG CC^T[ACGT]AGG
2536 CCTAAGG
Eco81I 486 CCTCAGG CC^T[ACGT]AGG
2536 CCTAAGG
FbaI 1156 TGATCA T^GATCA
3542 TGATCA
HgaI 120 GACGC GACGC(5/10)
409 GACGC
Hin1I 120 GACGCC G[GA]^CG[CT]C
210 GGCGCC
KspI 153 CCGCGG CCGC^GG
172 CCGCGG
MflI 1259 AGATCT [GA]^GATC[CT]
3494 GGATCT
MluNI 701 TGGCCA TGG^CCA
1589 TGGCCA
MscI 701 TGGCCA TGG^CCA
1589 TGGCCA
PshAI 1872 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2467 GACATAGGTC
SacII 153 CCGCGG CCGC^GG
172 CCGCGG
SmaI 97 CCCGGG CCC^GGG
441 CCCGGG
SphI 578 GCATGC GCATG^C
1622 GCATGC
SspI 1384 AATATT AAT^ATT
3934 AATATT
XhoII 1259 AGATCT [GA]^GATC[CT]
3494 GGATCT
XmaCI 97 CCCGGG C^CCGGG
441 CCCGGG
XmaI 97 CCCGGG C^CCGGG
441 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XmnI