##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AclI AgeI AscI AseI
AsnI AspI AviII BamHI BbeI
BbrPI BsiWI BspDI BsrFI BssHII
BssSI BstBI Cfr10I ClaI CpoI
DrdI EcoRI FseI FspI HpaI
KasI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SalI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SwaI Tth111I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 3262 GGTACC G^GTACC
AccIII 2403 TCCGGA T^CCGGA
AflII 2766 CTTAAG C^TTAAG
Alw44I 3776 GTGCAC G^TGCAC
Aor51HI 1718 AGCGCT AGC^GCT
ApaLI 3776 GTGCAC G^TGCAC
Asp718I 3262 GGTACC G^GTACC
AvrII 3135 CCTAGG C^CTAGG
BbvCI 2389 GCTGAGG CCTCAGC(-5/-2)
BcgI 3453 GCAGGCAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2766 CTTAAG C^TTAAG
BlnI 3135 CCTAGG C^CTAGG
BlpI 1423 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1423 GCTAAGC GC^T[ACGT]AGC
BsaAI 3210 TACGTG [CT]AC^GT[GA]
BsaBI 308 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2403 TCCGGA [AT]^CCGG[AT]
BseAI 2403 TCCGGA T^CCGGA
BspEI 2403 TCCGGA T^CCGGA
BsrBI 86 GAGCGG CCGCTC(-3/-3)
Bst1107I 1548 GTATAC GTA^TAC
BstZ17I 1548 GTATAC GTA^TAC
CelII 1423 GCTAAGC GC^T[ACGT]AGC
DraI 1954 TTTAAA TTT^AAA
DraIII 1500 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 89 CGGCCG C^GGCCG
EclXI 89 CGGCCG C^GGCCG
Eco47III 1718 AGCGCT AGC^GCT
Eco52I 89 CGGCCG C^GGCCG
HaeII 1718 AGCGCT [GA]GCGC^[CT]
HincII 991 GTCAAC GT[CT]^[GA]AC
HindII 991 GTCAAC GT[CT]^[GA]AC
HindIII 3736 AAGCTT A^AGCTT
KpnI 3262 GGTACC GGTAC^C
KspI 81 CCGCGG CCGC^GG
MamI 308 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2403 TCCGGA T^CCGGA
NheI 2904 GCTAGC G^CTAGC
PaeR7I 3999 CTCGAG C^TCGAG
SacII 81 CCGCGG CCGC^GG
SbfI 3397 CCTGCAGG CCTGCA^GG
SexAI 3848 ACCTGGT A^CC[AT]GGT
Sse8387I 3397 CCTGCAGG CCTGCA^GG
TfiI 4053 GAATC G^A[AT]TC
XhoI 3999 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 4 CGCG CG^CG
82 CGCG
AcsI 1951 GAATTT [GA]^AATT[CT]
2137 AAATTT
AflIII 1703 ACATGT A^C[GA][CT]GT
4339 ACATGT
AhdI 94 GACGCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2323 GACATTCTGTC
AocI 2271 CCTAAGG CC^T[ACGT]AGG
2527 CCTAAGG
ApaI 135 GGGCCC GGGCC^C
3554 GGGCCC
ApoI 1951 GAATTT [GA]^AATT[CT]
2137 AAATTT
AspEI 94 GACGCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2323 GACATTCTGTC
BalI 378 TGGCCA TGG^CCA
1738 TGGCCA
BbsI 1585 GAAGAC GAAGAC(2/6)
958 GTCTTC
BciVI 1263 GGATAC GTATCC(6/5)
3207 GGATAC
BclI 168 TGATCA T^GATCA
4403 TGATCA
BpuAI 1585 GAAGAC GAAGAC(2/6)
958 GTCTTC
BsiEI 89 CGGCCG CG[GA][CT]^CG
1879 CGACCG
BsmBI 1672 CGTCTC CGTCTC(1/5)
2006 GAGACG
BspHI 1835 TCATGA T^CATGA
4049 TCATGA
BspLU11I 1703 ACATGT A^CATGT
4339 ACATGT
BsrDI 1014 CATTGC GCAATG(2/0)
1612 CATTGC
BstEII 2872 GGTGACC G^GT[ACGT]ACC
3283 GGTCACC
BstPI 2872 GGTGACC G^GT[ACGT]ACC
3283 GGTCACC
BstUI 4 CGCG CG^CG
82 CGCG
BstXI 1729 CCAGGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4123 CCAGGGGCCTGG
Bsu36I 2271 CCTAAGG CC^T[ACGT]AGG
2527 CCTAAGG
Eam1105I 94 GACGCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2323 GACATTCTGTC
Eco81I 2271 CCTAAGG CC^T[ACGT]AGG
2527 CCTAAGG
EcoO65I 2872 GGTGACC G^GT[ACGT]ACC
3283 GGTCACC
EcoRV 832 GATATC GAT^ATC
4114 GATATC
FbaI 168 TGATCA T^GATCA
4403 TGATCA
MluNI 378 TGGCCA TGG^CCA
1738 TGGCCA
MscI 378 TGGCCA TGG^CCA
1738 TGGCCA
MvnI 4 CGCG CG^CG
82 CGCG
RcaI 1835 TCATGA T^CATGA
4049 TCATGA
SapI 2311 GAAGAGC GCTCTTC(1/4)
1245 GCTCTTC
SspI 388 AATATT AAT^ATT
2676 AATATT
TaqI 2118 TCGA T^CGA
4000 TCGA
TthHB8I 2118 TCGA T^CGA
4000 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AlwI AlwNI Asp700I AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BglI BglII BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspBI StuI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI XhoII XmnI