################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII AclI AgeI AscI AseI AsnI AspI AviII BamHI BbeI BbrPI BsiWI BspDI BsrFI BssHII BssSI BstBI Cfr10I ClaI CpoI DrdI EcoRI FseI FspI HpaI KasI MfeI MluI MunI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SalI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 3262 GGTACC G^GTACC AccIII 2403 TCCGGA T^CCGGA AflII 2766 CTTAAG C^TTAAG Alw44I 3776 GTGCAC G^TGCAC Aor51HI 1718 AGCGCT AGC^GCT ApaLI 3776 GTGCAC G^TGCAC Asp718I 3262 GGTACC G^GTACC AvrII 3135 CCTAGG C^CTAGG BbvCI 2389 GCTGAGG CCTCAGC(-5/-2) BcgI 3453 GCAGGCAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 2766 CTTAAG C^TTAAG BlnI 3135 CCTAGG C^CTAGG BlpI 1423 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1423 GCTAAGC GC^T[ACGT]AGC BsaAI 3210 TACGTG [CT]AC^GT[GA] BsaBI 308 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2403 TCCGGA [AT]^CCGG[AT] BseAI 2403 TCCGGA T^CCGGA BspEI 2403 TCCGGA T^CCGGA BsrBI 86 GAGCGG CCGCTC(-3/-3) Bst1107I 1548 GTATAC GTA^TAC BstZ17I 1548 GTATAC GTA^TAC CelII 1423 GCTAAGC GC^T[ACGT]AGC DraI 1954 TTTAAA TTT^AAA DraIII 1500 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 89 CGGCCG C^GGCCG EclXI 89 CGGCCG C^GGCCG Eco47III 1718 AGCGCT AGC^GCT Eco52I 89 CGGCCG C^GGCCG HaeII 1718 AGCGCT [GA]GCGC^[CT] HincII 991 GTCAAC GT[CT]^[GA]AC HindII 991 GTCAAC GT[CT]^[GA]AC HindIII 3736 AAGCTT A^AGCTT KpnI 3262 GGTACC GGTAC^C KspI 81 CCGCGG CCGC^GG MamI 308 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2403 TCCGGA T^CCGGA NheI 2904 GCTAGC G^CTAGC PaeR7I 3999 CTCGAG C^TCGAG SacII 81 CCGCGG CCGC^GG SbfI 3397 CCTGCAGG CCTGCA^GG SexAI 3848 ACCTGGT A^CC[AT]GGT Sse8387I 3397 CCTGCAGG CCTGCA^GG TfiI 4053 GAATC G^A[AT]TC XhoI 3999 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccII 4 CGCG CG^CG 82 CGCG AcsI 1951 GAATTT [GA]^AATT[CT] 2137 AAATTT AflIII 1703 ACATGT A^C[GA][CT]GT 4339 ACATGT AhdI 94 GACGCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2323 GACATTCTGTC AocI 2271 CCTAAGG CC^T[ACGT]AGG 2527 CCTAAGG ApaI 135 GGGCCC GGGCC^C 3554 GGGCCC ApoI 1951 GAATTT [GA]^AATT[CT] 2137 AAATTT AspEI 94 GACGCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2323 GACATTCTGTC BalI 378 TGGCCA TGG^CCA 1738 TGGCCA BbsI 1585 GAAGAC GAAGAC(2/6) 958 GTCTTC BciVI 1263 GGATAC GTATCC(6/5) 3207 GGATAC BclI 168 TGATCA T^GATCA 4403 TGATCA BpuAI 1585 GAAGAC GAAGAC(2/6) 958 GTCTTC BsiEI 89 CGGCCG CG[GA][CT]^CG 1879 CGACCG BsmBI 1672 CGTCTC CGTCTC(1/5) 2006 GAGACG BspHI 1835 TCATGA T^CATGA 4049 TCATGA BspLU11I 1703 ACATGT A^CATGT 4339 ACATGT BsrDI 1014 CATTGC GCAATG(2/0) 1612 CATTGC BstEII 2872 GGTGACC G^GT[ACGT]ACC 3283 GGTCACC BstPI 2872 GGTGACC G^GT[ACGT]ACC 3283 GGTCACC BstUI 4 CGCG CG^CG 82 CGCG BstXI 1729 CCAGGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4123 CCAGGGGCCTGG Bsu36I 2271 CCTAAGG CC^T[ACGT]AGG 2527 CCTAAGG Eam1105I 94 GACGCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2323 GACATTCTGTC Eco81I 2271 CCTAAGG CC^T[ACGT]AGG 2527 CCTAAGG EcoO65I 2872 GGTGACC G^GT[ACGT]ACC 3283 GGTCACC EcoRV 832 GATATC GAT^ATC 4114 GATATC FbaI 168 TGATCA T^GATCA 4403 TGATCA MluNI 378 TGGCCA TGG^CCA 1738 TGGCCA MscI 378 TGGCCA TGG^CCA 1738 TGGCCA MvnI 4 CGCG CG^CG 82 CGCG RcaI 1835 TCATGA T^CATGA 4049 TCATGA SapI 2311 GAAGAGC GCTCTTC(1/4) 1245 GCTCTTC SspI 388 AATATT AAT^ATT 2676 AATATT TaqI 2118 TCGA T^CGA 4000 TCGA TthHB8I 2118 TCGA T^CGA 4000 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccI AciI AcyI AfaI AluI AlwI AlwNI Asp700I AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BglI BglII BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspBI StuI StyI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII XmnI