##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AclI AcyI AhdI
Aor51HI ApaI AscI Asp700I Asp718I
AspEI AspI AviII BbeI BcgI
BlpI Bpu1102I BsaBI BsaHI BsmBI
BspDI BsrDI Bst1107I BstBI BstZ17I
CelII ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
FseI FspI Hin1I HpaI KasI
KpnI KspI MamI MfeI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NspV PacI PflFI PmeI
Psp1406I RsrII SacII SapI SbfI
ScaI SfiI SfoI SfuI SnaBI
SpeI Sse8387I SspI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 342 GTCGAC GT^[AC][GT]AC
AgeI 2547 ACCGGT A^CCGGT
Alw44I 1347 GTGCAC G^TGCAC
AocI 2418 CCTCAGG CC^T[ACGT]AGG
ApaLI 1347 GTGCAC G^TGCAC
AseI 1431 ATTAAT AT^TAAT
AsnI 1431 ATTAAT AT^TAAT
BamHI 975 GGATCC G^GATCC
BbrPI 4530 CACGTG CAC^GTG
BciVI 3194 GTATCC GTATCC(6/5)
BglI 1455 GCCGTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 4530 CACGTG [CT]AC^GT[GA]
BsiEI 74 CGATCG CG[GA][CT]^CG
BsiWI 602 CGTACG C^GTACG
BsrFI 2547 ACCGGT [GA]^CCGG[CT]
BssHII 96 GCGCGC G^CGCGC
Bsu36I 2418 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2547 ACCGGT [GA]^CCGG[CT]
Eco81I 2418 CCTCAGG CC^T[ACGT]AGG
EcoRV 489 GATATC GAT^ATC
EcoT22I 1764 ATGCAT ATGCA^T
HaeII 3637 AGCGCC [GA]GCGC^[CT]
HincII 342 GTCGAC GT[CT]^[GA]AC
HindII 342 GTCGAC GT[CT]^[GA]AC
HindIII 4328 AAGCTT A^AGCTT
MluI 889 ACGCGT A^CGCGT
NcoI 1172 CCATGG C^CATGG
NsiI 1764 ATGCAT ATGCA^T
PaeR7I 403 CTCGAG C^TCGAG
PinAI 2547 ACCGGT A^CCGGT
PmaCI 4530 CACGTG CAC^GTG
PmlI 4530 CACGTG CAC^GTG
PshAI 1978 GACGTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1431 ATTAAT AT^TAAT
PvuI 74 CGATCG CGAT^CG
SacI 2619 GAGCTC GAGCT^C
SalI 342 GTCGAC G^TCGAC
SexAI 1735 ACCTGGT A^CC[AT]GGT
SgrAI 2546 CACCGGTG C[GA]^CCGG[CT]G
SmaI 2653 CCCGGG CCC^GGG
SphI 176 GCATGC GCATG^C
StuI 1244 AGGCCT AGG^CCT
SwaI 4274 ATTTAAAT ATTT^AAAT
XbaI 218 TCTAGA T^CTAGA
XhoI 403 CTCGAG C^TCGAG
XmaCI 2653 CCCGGG C^CCGGG
XmaI 2653 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccIII 1294 TCCGGA T^CCGGA
3092 TCCGGA
AcsI 4272 AAATTT [GA]^AATT[CT]
4278 AAATTC
AflII 1166 CTTAAG C^TTAAG
3699 CTTAAG
ApoI 4272 AAATTT [GA]^AATT[CT]
4278 AAATTC
AvrII 1698 CCTAGG C^CTAGG
1797 CCTAGG
BalI 2978 TGGCCA TGG^CCA
4614 TGGCCA
BbvCI 4729 CCTCAGC CCTCAGC(-5/-2)
4559 GCTGAGG
BfrI 1166 CTTAAG C^TTAAG
3699 CTTAAG
BlnI 1698 CCTAGG C^CTAGG
1797 CCTAGG
BseAI 1294 TCCGGA T^CCGGA
3092 TCCGGA
BsmI 2183 GAATGC GAATGC(1/-1)
3605 GCATTC
BspEI 1294 TCCGGA T^CCGGA
3092 TCCGGA
BsrBI 3221 CCGCTC CCGCTC(-3/-3)
2658 GAGCGG
BstAPI 176 GCATGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2878 GCACCCAGTGC
DraI 4224 TTTAAA TTT^AAA
4275 TTTAAA
MluNI 2978 TGGCCA TGG^CCA
4614 TGGCCA
MroI 1294 TCCGGA T^CCGGA
3092 TCCGGA
MscI 2978 TGGCCA TGG^CCA
4614 TGGCCA
NheI 3380 GCTAGC G^CTAGC
4700 GCTAGC
PflMI 671 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2990 CCACATGATGG
PpuMI 1701 AGGACCT [GA]G^G[AT]CC[CT]
2511 GGGTCCT
Van91I 671 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2990 CCACATGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbsI BbvI BclI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspHI
BspLU11I BspMI BsrGI BsrI BssKI
BssSI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII