################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII AccI AccIII AclI AflII AhdI AscI AseI AsnI Asp700I AspEI AspI BbvCI BcgI BciVI BclI BfrI BsaBI BseAI BsiWI BsmI BspDI BspEI BspHI BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DrdI Eam1105I EcoO65I FbaI FseI MamI MfeI MluI MroI MunI NdeI NotI NruI NspV PacI PaeR7I PflFI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI Sse8387I SspI SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AcsI 4191 GAATTC [GA]^AATT[CT] AflIII 3124 ACATGT A^C[GA][CT]GT AgeI 3459 ACCGGT A^CCGGT ApoI 4191 GAATTC [GA]^AATT[CT] AviII 1275 TGCGCA TGC^GCA AvrII 2176 CCTAGG C^CTAGG BlnI 2176 CCTAGG C^CTAGG BsiEI 20 CGGCCG CG[GA][CT]^CG Bsp1407I 4399 TGTACA T^GTACA BspLU11I 3124 ACATGT A^CATGT BsrDI 954 CATTGC GCAATG(2/0) BsrGI 4399 TGTACA T^GTACA DraI 299 TTTAAA TTT^AAA EagI 20 CGGCCG C^GGCCG EclXI 20 CGGCCG C^GGCCG Eco52I 20 CGGCCG C^GGCCG EcoRI 4191 GAATTC G^AATTC EcoRV 3952 GATATC GAT^ATC EcoT22I 2153 ATGCAT ATGCA^T FspI 1275 TGCGCA TGC^GCA HpaI 3463 GTTAAC GTT^AAC KspI 579 CCGCGG CCGC^GG NheI 3192 GCTAGC G^CTAGC NsiI 2153 ATGCAT ATGCA^T PinAI 3459 ACCGGT A^CCGGT SacII 579 CCGCGG CCGC^GG SapI 3616 GCTCTTC GCTCTTC(1/4) SpeI 3164 ACTAGT A^CTAGT SphI 2151 GCATGC GCATG^C SspBI 4399 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### Aor51HI 2624 AGCGCT AGC^GCT 3283 AGCGCT BamHI 1502 GGATCC G^GATCC 3092 GGATCC BbeI 116 GGCGCC GGCGC^C 3388 GGCGCC BbrPI 2896 CACGTG CAC^GTG 3198 CACGTG BglII 1348 AGATCT A^GATCT 3176 AGATCT BsaWI 2600 ACCGGA [AT]^CCGG[AT] 3459 ACCGGT BssHII 70 GCGCGC G^CGCGC 2425 GCGCGC DraIII 1778 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 3937 CACAGTGTG Eco47III 2624 AGCGCT AGC^GCT 3283 AGCGCT HincII 1482 GTTGAC GT[CT]^[GA]AC 3463 GTTAAC HindII 1482 GTTGAC GT[CT]^[GA]AC 3463 GTTAAC HindIII 2339 AAGCTT A^AGCTT 3824 AAGCTT KasI 116 GGCGCC G^GCGCC 3388 GGCGCC NaeI 18 GCCGGC GCC^GGC 710 GCCGGC NarI 116 GGCGCC GG^CGCC 3388 GGCGCC NgoMIV 18 GCCGGC G^CCGGC 710 GCCGGC PflMI 3075 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3936 CCACAGTGTGG PmaCI 2896 CACGTG CAC^GTG 3198 CACGTG PmlI 2896 CACGTG CAC^GTG 3198 CACGTG SfoI 116 GGCGCC GGC^GCC 3388 GGCGCC Van91I 3075 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3936 CCACAGTGTGG XbaI 2142 TCTAGA T^CTAGA 3883 TCTAGA XcmI 2806 CCAGGCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3511 CCAGGGATAAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 137 enzymes) # ######################################### Acc65I AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI Asp718I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PshAI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI