##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AhdI AscI AseI AsnI Asp700I
AspEI AspI BbvCI BcgI BciVI
BclI BfrI BsaBI BseAI BsiWI
BsmI BspDI BspEI BspHI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DrdI Eam1105I EcoO65I
FbaI FseI MamI MfeI MluI
MroI MunI NdeI NotI NruI
NspV PacI PaeR7I PflFI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI Sse8387I SspI SwaI
Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 4191 GAATTC [GA]^AATT[CT]
AflIII 3124 ACATGT A^C[GA][CT]GT
AgeI 3459 ACCGGT A^CCGGT
ApoI 4191 GAATTC [GA]^AATT[CT]
AviII 1275 TGCGCA TGC^GCA
AvrII 2176 CCTAGG C^CTAGG
BlnI 2176 CCTAGG C^CTAGG
BsiEI 20 CGGCCG CG[GA][CT]^CG
Bsp1407I 4399 TGTACA T^GTACA
BspLU11I 3124 ACATGT A^CATGT
BsrDI 954 CATTGC GCAATG(2/0)
BsrGI 4399 TGTACA T^GTACA
DraI 299 TTTAAA TTT^AAA
EagI 20 CGGCCG C^GGCCG
EclXI 20 CGGCCG C^GGCCG
Eco52I 20 CGGCCG C^GGCCG
EcoRI 4191 GAATTC G^AATTC
EcoRV 3952 GATATC GAT^ATC
EcoT22I 2153 ATGCAT ATGCA^T
FspI 1275 TGCGCA TGC^GCA
HpaI 3463 GTTAAC GTT^AAC
KspI 579 CCGCGG CCGC^GG
NheI 3192 GCTAGC G^CTAGC
NsiI 2153 ATGCAT ATGCA^T
PinAI 3459 ACCGGT A^CCGGT
SacII 579 CCGCGG CCGC^GG
SapI 3616 GCTCTTC GCTCTTC(1/4)
SpeI 3164 ACTAGT A^CTAGT
SphI 2151 GCATGC GCATG^C
SspBI 4399 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Aor51HI 2624 AGCGCT AGC^GCT
3283 AGCGCT
BamHI 1502 GGATCC G^GATCC
3092 GGATCC
BbeI 116 GGCGCC GGCGC^C
3388 GGCGCC
BbrPI 2896 CACGTG CAC^GTG
3198 CACGTG
BglII 1348 AGATCT A^GATCT
3176 AGATCT
BsaWI 2600 ACCGGA [AT]^CCGG[AT]
3459 ACCGGT
BssHII 70 GCGCGC G^CGCGC
2425 GCGCGC
DraIII 1778 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
3937 CACAGTGTG
Eco47III 2624 AGCGCT AGC^GCT
3283 AGCGCT
HincII 1482 GTTGAC GT[CT]^[GA]AC
3463 GTTAAC
HindII 1482 GTTGAC GT[CT]^[GA]AC
3463 GTTAAC
HindIII 2339 AAGCTT A^AGCTT
3824 AAGCTT
KasI 116 GGCGCC G^GCGCC
3388 GGCGCC
NaeI 18 GCCGGC GCC^GGC
710 GCCGGC
NarI 116 GGCGCC GG^CGCC
3388 GGCGCC
NgoMIV 18 GCCGGC G^CCGGC
710 GCCGGC
PflMI 3075 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3936 CCACAGTGTGG
PmaCI 2896 CACGTG CAC^GTG
3198 CACGTG
PmlI 2896 CACGTG CAC^GTG
3198 CACGTG
SfoI 116 GGCGCC GGC^GCC
3388 GGCGCC
Van91I 3075 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3936 CCACAGTGTGG
XbaI 2142 TCTAGA T^CTAGA
3883 TCTAGA
XcmI 2806 CCAGGCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3511 CCAGGGATAAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI Asp718I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PshAI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI