##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
ApaI AscI Asp718I AspEI AspI
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BlnI BlpI Bpu1102I
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BsrGI Bst1107I BstZ17I CelII
ClaI CpoI EagI Eam1105I EclXI
Eco52I FseI FspI KasI KpnI
MfeI MluI MroI MunI NarI
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacI SalI
SfoI SgrAI SmaI SnaBI SspBI
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 435 GACGTC GACGT^C
AflII 4938 CTTAAG C^TTAAG
Aor51HI 142 AGCGCT AGC^GCT
BalI 3101 TGGCCA TGG^CCA
BcgI 2146 GCATTTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4173 GGATAC GTATCC(6/5)
BclI 4816 TGATCA T^GATCA
BfrI 4938 CTTAAG C^TTAAG
BglI 101 GCCGCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 5035 AGATCT A^GATCT
BsaAI 800 CACGTA [CT]AC^GT[GA]
BsaBI 4446 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 228 CGGTCG CG[GA][CT]^CG
BsrBI 1724 GAGCGG CCGCTC(-3/-3)
BsrFI 193 GCCGGC [GA]^CCGG[CT]
BstAPI 1069 GCAGTGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 556 TTCGAA TT^CGAA
BstEII 3122 GGTCACC G^GT[ACGT]ACC
BstPI 3122 GGTCACC G^GT[ACGT]ACC
Cfr10I 193 GCCGGC [GA]^CCGG[CT]
DrdI 1976 GACAGTTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 142 AGCGCT AGC^GCT
EcoNI 785 CCTGAATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3122 GGTCACC G^GT[ACGT]ACC
EcoRI 4915 GAATTC G^AATTC
EcoRV 2061 GATATC GAT^ATC
FbaI 4816 TGATCA T^GATCA
MamI 4446 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3101 TGGCCA TGG^CCA
MscI 3101 TGGCCA TGG^CCA
NaeI 193 GCCGGC GCC^GGC
NgoMIV 193 GCCGGC G^CCGGC
NspV 556 TTCGAA TT^CGAA
PshAI 4258 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1142 GAAGAGC GCTCTTC(1/4)
SbfI 4880 CCTGCAGG CCTGCA^GG
SexAI 1267 ACCAGGT A^CC[AT]GGT
SfiI 100 GGCCGCGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 556 TTCGAA TT^CGAA
SmlI 4938 CTTAAG C^T[CT][GA]AG
SpeI 4308 ACTAGT A^CTAGT
Sse8387I 4880 CCTGCAGG CCTGCA^GG
XcmI 1183 CCAAATTGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 2178 GTCTAC GT^[AC][GT]AC
2683 GTCTAC
Alw44I 3442 GTGCAC G^TGCAC
3675 GTGCAC
AocI 584 CCTGAGG CC^T[ACGT]AGG
1160 CCTGAGG
ApaLI 3442 GTGCAC G^TGCAC
3675 GTGCAC
BcnI 36 CCCGG CC^[GC]GG
749 CCGGG
BsmBI 4132 CGTCTC CGTCTC(1/5)
270 GAGACG
BspHI 400 TCATGA T^CATGA
1775 TCATGA
BsrDI 351 CATTGC GCAATG(2/0)
2465 CATTGC
BssHII 78 GCGCGC G^CGCGC
129 GCGCGC
BssSI 73 CACGAG CACGAG(-5/-1)
1568 CACGAG
BstXI 1183 CCAAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3414 CCAGAATTGTGG
Bsu36I 584 CCTGAGG CC^T[ACGT]AGG
1160 CCTGAGG
DraII 646 AGGTCCT [GA]G^G[ACGT]CC[CT]
3696 AGGACCC
DraIII 474 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1266 CACCAGGTG
EaeI 99 TGGCCG [CT]^GGCC[GA]
3101 TGGCCA
Eco81I 584 CCTGAGG CC^T[ACGT]AGG
1160 CCTGAGG
EcoO109I 646 AGGTCCT [GA]G^G[ACGT]CC[CT]
3696 AGGACCC
HindIII 2315 AAGCTT A^AGCTT
4989 AAGCTT
HpaI 2791 GTTAAC GTT^AAC
4362 GTTAAC
NciI 36 CCCGG CC^[GC]GG
749 CCGGG
NcoI 96 CCATGG C^CATGG
2478 CCATGG
NdeI 2415 CATATG CA^TATG
3802 CATATG
PpuMI 646 AGGTCCT [GA]G^G[AT]CC[CT]
3696 AGGACCC
PvuII 661 CAGCTG CAG^CTG
4231 CAGCTG
RcaI 400 TCATGA T^CATGA
1775 TCATGA
StuI 3278 AGGCCT AGG^CCT
4166 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AseI AsnI Asp700I AspHI AvaI
AvaII BanI BbsI BbvI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspLU11I BspMI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DsaI EarI EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PshBI
PstI RsaI SacII Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI