##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AcyI AgeI Alw44I
ApaI ApaLI AscI Asp718I AspI
AviII AvrII BamHI BbeI BclI
BlnI BsaBI BsaHI BsiWI Bsp1407I
BspDI BspLU11I BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI EagI EclXI Eco52I
EcoNI EcoO65I EcoRV FbaI FseI
FspI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NarI NdeI NotI NruI
NspV PacI PflFI PinAI RsrII
SacII SalI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AclI 1647 AACGTT AA^CGTT
AflIII 2410 ACGTGT A^C[GA][CT]GT
AocI 2833 CCTCAGG CC^T[ACGT]AGG
AseI 2971 ATTAAT AT^TAAT
AsnI 2971 ATTAAT AT^TAAT
BalI 2802 TGGCCA TGG^CCA
BbrPI 2409 CACGTG CAC^GTG
BbvCI 2315 CCTCAGC CCTCAGC(-5/-2)
BciVI 1098 GGATAC GTATCC(6/5)
BglI 2159 GCCAAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2409 CACGTG [CT]AC^GT[GA]
BsiEI 835 CGATCG CG[GA][CT]^CG
BsmI 1608 GAATGC GAATGC(1/-1)
BspHI 4086 TCATGA T^CATGA
BsrFI 2267 GCCGGC [GA]^CCGG[CT]
BssHII 5 GCGCGC G^CGCGC
BstAPI 742 GCAAAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2833 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2267 GCCGGC [GA]^CCGG[CT]
Eco81I 2833 CCTCAGG CC^T[ACGT]AGG
EcoRI 4151 GAATTC G^AATTC
EcoT22I 91 ATGCAT ATGCA^T
HincII 616 GTCAAC GT[CT]^[GA]AC
HindII 616 GTCAAC GT[CT]^[GA]AC
MluNI 2802 TGGCCA TGG^CCA
MscI 2802 TGGCCA TGG^CCA
NaeI 2267 GCCGGC GCC^GGC
NgoMIV 2267 GCCGGC G^CCGGC
NsiI 91 ATGCAT ATGCA^T
PflMI 3003 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2409 CACGTG CAC^GTG
PmeI 861 GTTTAAAC GTTT^AAAC
PmlI 2409 CACGTG CAC^GTG
PshBI 2971 ATTAAT AT^TAAT
Psp1406I 1647 AACGTT AA^CGTT
PvuI 835 CGATCG CGAT^CG
RcaI 4086 TCATGA T^CATGA
SacI 1368 GAGCTC GAGCT^C
SbfI 1953 CCTGCAGG CCTGCA^GG
ScaI 4109 AGTACT AGT^ACT
SmaI 1468 CCCGGG CCC^GGG
Sse8387I 1953 CCTGCAGG CCTGCA^GG
Van91I 3003 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1468 CCCGGG C^CCGGG
XmaI 1468 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 795 GTCTAC GT^[AC][GT]AC
2346 GTCTAC
AflII 3169 CTTAAG C^TTAAG
3706 CTTAAG
AhdI 934 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1393 GACATTACGTC
Aor51HI 1768 AGCGCT AGC^GCT
3254 AGCGCT
Asp700I 3112 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4147 GAAAGAATTC
AspEI 934 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1393 GACATTACGTC
BfrI 3169 CTTAAG C^TTAAG
3706 CTTAAG
BsrBI 1035 CCGCTC CCGCTC(-3/-3)
671 GAGCGG
BsrDI 3546 CATTGC GCAATG(2/0)
1661 GCAATG
Eam1105I 934 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1393 GACATTACGTC
Eco47III 1768 AGCGCT AGC^GCT
3254 AGCGCT
HgaI 2 GACGC GACGC(5/10)
2401 GCGTC
NspI 2036 ACATGC [GA]CATG^[CT]
4290 GCATGT
PaeR7I 2341 CTCGAG C^TCGAG
3323 CTCGAG
PshAI 584 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3829 GACAGGAGTC
PvuII 3086 CAGCTG CAG^CTG
3485 CAGCTG
SapI 668 GAAGAGC GCTCTTC(1/4)
1037 GCTCTTC
XbaI 1272 TCTAGA T^CTAGA
2299 TCTAGA
XcmI 2138 CCAGGGGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2243 CCATGCCGTCCCTGG
XhoI 2341 CTCGAG C^TCGAG
3323 CTCGAG
XmnI 3112 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4147 GAAAGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AccIII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcgI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MroI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NheI
NlaIII NlaIV PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII