##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AclI AcsI AflII AflIII
AgeI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I AviII BclI
BfrI BglII BsaBI BsiEI BsiWI
BspDI BspHI BspLU11I BsrBI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI MamI
MfeI MluI MunI NaeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SalI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI SspI StuI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 222 GGTACC G^GTACC
AccI 401 GTAGAC GT^[AC][GT]AC
AccIII 2933 TCCGGA T^CCGGA
AhdI 2217 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 908 GTGCAC G^TGCAC
ApaLI 908 GTGCAC G^TGCAC
Asp718I 222 GGTACC G^GTACC
AspEI 2217 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3807 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1882 TGGCCA TGG^CCA
BbrPI 1190 CACGTG CAC^GTG
BbsI 1941 GTCTTC GAAGAC(2/6)
BbvCI 2386 CCTCAGC CCTCAGC(-5/-2)
BcgI 2754 GCAGAGGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2951 GTATCC GTATCC(6/5)
BglI 2078 GCCTTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1941 GTCTTC GAAGAC(2/6)
BsaWI 2933 TCCGGA [AT]^CCGG[AT]
BseAI 2933 TCCGGA T^CCGGA
BsmI 1018 GAATGC GAATGC(1/-1)
Bsp1407I 924 TGTACA T^GTACA
BspEI 2933 TCCGGA T^CCGGA
BsrDI 987 GCAATG GCAATG(2/0)
BsrFI 1170 ACCGGC [GA]^CCGG[CT]
BsrGI 924 TGTACA T^GTACA
BssSI 1120 CACGAG CACGAG(-5/-1)
Cfr10I 1170 ACCGGC [GA]^CCGG[CT]
Eam1105I 2217 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HindIII 3625 AAGCTT A^AGCTT
KpnI 222 GGTACC GGTAC^C
KspI 878 CCGCGG CCGC^GG
MluNI 1882 TGGCCA TGG^CCA
MroI 2933 TCCGGA T^CCGGA
MscI 1882 TGGCCA TGG^CCA
NdeI 3744 CATATG CA^TATG
PflFI 3807 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3659 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1190 CACGTG CAC^GTG
PmlI 1190 CACGTG CAC^GTG
SacII 878 CCGCGG CCGC^GG
SbfI 1308 CCTGCAGG CCTGCA^GG
ScaI 445 AGTACT AGT^ACT
SmaI 585 CCCGGG CCC^GGG
Sse8387I 1308 CCTGCAGG CCTGCA^GG
SspBI 924 TGTACA T^GTACA
TaqI 3823 TCGA T^CGA
Tth111I 3807 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 3823 TCGA T^CGA
Van91I 3659 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 585 CCCGGG C^CCGGG
XmaI 585 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AocI 2417 CCTAAGG CC^T[ACGT]AGG
2926 CCTAAGG
AvaI 585 CCCGGG C^[CT]CG[GA]G
3094 CTCGGG
AvrII 1272 CCTAGG C^CTAGG
2771 CCTAGG
BamHI 607 GGATCC G^GATCC
2643 GGATCC
BlnI 1272 CCTAGG C^CTAGG
2771 CCTAGG
BlpI 1848 GCTGAGC GC^T[ACGT]AGC
3405 GCTAAGC
Bpu1102I 1848 GCTGAGC GC^T[ACGT]AGC
3405 GCTAAGC
BsaAI 704 TACGTG [CT]AC^GT[GA]
1190 CACGTG
BsmBI 1423 CGTCTC CGTCTC(1/5)
3214 GAGACG
BsoBI 585 CCCGGG C^[CT]CG[GA]G
3094 CTCGGG
BstEII 1508 GGTGACC G^GT[ACGT]ACC
3687 GGTGACC
BstPI 1508 GGTGACC G^GT[ACGT]ACC
3687 GGTGACC
Bsu36I 2417 CCTAAGG CC^T[ACGT]AGG
2926 CCTAAGG
CelII 1848 GCTGAGC GC^T[ACGT]AGC
3405 GCTAAGC
DraI 3897 TTTAAA TTT^AAA
4016 TTTAAA
DraIII 486 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
3028 CACAAGGTG
Eco81I 2417 CCTAAGG CC^T[ACGT]AGG
2926 CCTAAGG
EcoO65I 1508 GGTGACC G^GT[ACGT]ACC
3687 GGTGACC
HgaI 2004 GACGC GACGC(5/10)
1540 GCGTC
NcoI 2179 CCATGG C^CATGG
3861 CCATGG
NheI 178 GCTAGC G^CTAGC
3669 GCTAGC
PshAI 2369 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3690 GACCAGGGTC
SacI 1337 GAGCTC GAGCT^C
2523 GAGCTC
SapI 1736 GCTCTTC GCTCTTC(1/4)
1773 GCTCTTC
XcmI 291 CCAAGGGTATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2238 CCAGGGCTAGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaII BanI
BanII BbeI BbvI BcnI BfaI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII