################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII AclI AcsI AflII AflIII AgeI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I AviII BclI BfrI BglII BsaBI BsiEI BsiWI BspDI BspHI BspLU11I BsrBI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI MamI MfeI MluI MunI NaeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI SspI StuI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 222 GGTACC G^GTACC AccI 401 GTAGAC GT^[AC][GT]AC AccIII 2933 TCCGGA T^CCGGA AhdI 2217 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 908 GTGCAC G^TGCAC ApaLI 908 GTGCAC G^TGCAC Asp718I 222 GGTACC G^GTACC AspEI 2217 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3807 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1882 TGGCCA TGG^CCA BbrPI 1190 CACGTG CAC^GTG BbsI 1941 GTCTTC GAAGAC(2/6) BbvCI 2386 CCTCAGC CCTCAGC(-5/-2) BcgI 2754 GCAGAGGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2951 GTATCC GTATCC(6/5) BglI 2078 GCCTTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 1941 GTCTTC GAAGAC(2/6) BsaWI 2933 TCCGGA [AT]^CCGG[AT] BseAI 2933 TCCGGA T^CCGGA BsmI 1018 GAATGC GAATGC(1/-1) Bsp1407I 924 TGTACA T^GTACA BspEI 2933 TCCGGA T^CCGGA BsrDI 987 GCAATG GCAATG(2/0) BsrFI 1170 ACCGGC [GA]^CCGG[CT] BsrGI 924 TGTACA T^GTACA BssSI 1120 CACGAG CACGAG(-5/-1) Cfr10I 1170 ACCGGC [GA]^CCGG[CT] Eam1105I 2217 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HindIII 3625 AAGCTT A^AGCTT KpnI 222 GGTACC GGTAC^C KspI 878 CCGCGG CCGC^GG MluNI 1882 TGGCCA TGG^CCA MroI 2933 TCCGGA T^CCGGA MscI 1882 TGGCCA TGG^CCA NdeI 3744 CATATG CA^TATG PflFI 3807 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3659 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1190 CACGTG CAC^GTG PmlI 1190 CACGTG CAC^GTG SacII 878 CCGCGG CCGC^GG SbfI 1308 CCTGCAGG CCTGCA^GG ScaI 445 AGTACT AGT^ACT SmaI 585 CCCGGG CCC^GGG Sse8387I 1308 CCTGCAGG CCTGCA^GG SspBI 924 TGTACA T^GTACA TaqI 3823 TCGA T^CGA Tth111I 3807 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 3823 TCGA T^CGA Van91I 3659 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 585 CCCGGG C^CCGGG XmaI 585 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AocI 2417 CCTAAGG CC^T[ACGT]AGG 2926 CCTAAGG AvaI 585 CCCGGG C^[CT]CG[GA]G 3094 CTCGGG AvrII 1272 CCTAGG C^CTAGG 2771 CCTAGG BamHI 607 GGATCC G^GATCC 2643 GGATCC BlnI 1272 CCTAGG C^CTAGG 2771 CCTAGG BlpI 1848 GCTGAGC GC^T[ACGT]AGC 3405 GCTAAGC Bpu1102I 1848 GCTGAGC GC^T[ACGT]AGC 3405 GCTAAGC BsaAI 704 TACGTG [CT]AC^GT[GA] 1190 CACGTG BsmBI 1423 CGTCTC CGTCTC(1/5) 3214 GAGACG BsoBI 585 CCCGGG C^[CT]CG[GA]G 3094 CTCGGG BstEII 1508 GGTGACC G^GT[ACGT]ACC 3687 GGTGACC BstPI 1508 GGTGACC G^GT[ACGT]ACC 3687 GGTGACC Bsu36I 2417 CCTAAGG CC^T[ACGT]AGG 2926 CCTAAGG CelII 1848 GCTGAGC GC^T[ACGT]AGC 3405 GCTAAGC DraI 3897 TTTAAA TTT^AAA 4016 TTTAAA DraIII 486 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG 3028 CACAAGGTG Eco81I 2417 CCTAAGG CC^T[ACGT]AGG 2926 CCTAAGG EcoO65I 1508 GGTGACC G^GT[ACGT]ACC 3687 GGTGACC HgaI 2004 GACGC GACGC(5/10) 1540 GCGTC NcoI 2179 CCATGG C^CATGG 3861 CCATGG NheI 178 GCTAGC G^CTAGC 3669 GCTAGC PshAI 2369 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3690 GACCAGGGTC SacI 1337 GAGCTC GAGCT^C 2523 GAGCTC SapI 1736 GCTCTTC GCTCTTC(1/4) 1773 GCTCTTC XcmI 291 CCAAGGGTATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2238 CCAGGGCTAGAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaII BanI BanII BbeI BbvI BcnI BfaI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII