##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI Alw44I ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AviII BalI BbeI BbsI BciVI
BfrI BglI BpuAI BsaI BsiWI
BspDI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DraIII EagI
EclXI Eco52I EcoO65I EcoRI FseI
FspI HindIII HpaI KasI KpnI
MfeI MluI MluNI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI Tth111I XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 4376 TCCGGA T^CCGGA
AcyI 2206 GACGCC G[GA]^CG[CT]C
AflIII 4235 ACATGT A^C[GA][CT]GT
AhdI 3610 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1124 GGGCCC GGGCC^C
AspEI 3610 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 802 CACGTG CAC^GTG
BcgI 2519 CGAATGTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2061 TGATCA T^GATCA
BglII 1058 AGATCT A^GATCT
BlpI 2320 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2320 GCTCAGC GC^T[ACGT]AGC
BsaAI 802 CACGTG [CT]AC^GT[GA]
BsaBI 2789 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2206 GACGCC G[GA]^CG[CT]C
BsaWI 4376 TCCGGA [AT]^CCGG[AT]
BseAI 4376 TCCGGA T^CCGGA
BsiEI 284 CGGTCG CG[GA][CT]^CG
BsmI 1687 GAATGC GAATGC(1/-1)
Bsp1407I 2011 TGTACA T^GTACA
BspEI 4376 TCCGGA T^CCGGA
BspLU11I 4235 ACATGT A^CATGT
BsrDI 8 CATTGC GCAATG(2/0)
BsrGI 2011 TGTACA T^GTACA
CelII 2320 GCTCAGC GC^T[ACGT]AGC
EaeI 390 TGGCCG [CT]^GGCC[GA]
Eam1105I 3610 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1041 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3178 GATATC GAT^ATC
FbaI 2061 TGATCA T^GATCA
Hin1I 2206 GACGCC G[GA]^CG[CT]C
KspI 1673 CCGCGG CCGC^GG
MamI 2789 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4376 TCCGGA T^CCGGA
NcoI 3426 CCATGG C^CATGG
PmaCI 802 CACGTG CAC^GTG
PmlI 802 CACGTG CAC^GTG
PshAI 790 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1898 CTGCAG CTGCA^G
SacI 27 GAGCTC GAGCT^C
SacII 1673 CCGCGG CCGC^GG
SapI 1383 GAAGAGC GCTCTTC(1/4)
ScaI 3796 AGTACT AGT^ACT
SphI 1710 GCATGC GCATG^C
SspBI 2011 TGTACA T^GTACA
SspI 2934 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 1879 GTAGAC GT^[AC][GT]AC
3434 GTAGAC
Aor51HI 543 AGCGCT AGC^GCT
2815 AGCGCT
AvrII 3194 CCTAGG C^CTAGG
4207 CCTAGG
BamHI 2074 GGATCC G^GATCC
3210 GGATCC
BanI 364 GGTGCC G^G[CT][GA]CC
1482 GGCACC
BlnI 3194 CCTAGG C^CTAGG
4207 CCTAGG
BsmBI 3243 CGTCTC CGTCTC(1/5)
2204 GAGACG
BspHI 266 TCATGA T^CATGA
3075 TCATGA
BspMI 1355 ACCTGC ACCTGC(4/8)
3626 ACCTGC
BstXI 512 CCACTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1332 CCAACAGCCTGG
Eco47III 543 AGCGCT AGC^GCT
2815 AGCGCT
FauI 993 CCCGC CCCGC(4/6)
1007 CCCGC
HgaI 2206 GACGC GACGC(5/10)
2748 GACGC
PpuMI 3190 GGGACCT [GA]G^G[AT]CC[CT]
3292 AGGACCT
RcaI 266 TCATGA T^CATGA
3075 TCATGA
SfcI 1877 CTGTAG C^T[GA][CT]AG
1898 CTGCAG
StuI 2562 AGGCCT AGG^CCT
4204 AGGCCT
XbaI 3770 TCTAGA T^CTAGA
3979 TCTAGA
XcmI 3489 CCAGTTATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3994 CCACCTGAGTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI AspHI AvaI AvaII
BanII BbvCI BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DrdI DsaI EarI
Eco81I EcoO109I EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmaCI XmaI