################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BciVI BclI BfrI BlnI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI Eam1105I Eco47III EcoRI EcoRV FbaI FseI FspI HincII HindII HpaI KpnI KspI MamI MfeI MluI MroI MunI NdeI NotI NspV PacI PaeR7I PflFI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SfuI SgrAI SnaBI SpeI SphI SspBI StuI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AcsI 4072 AAATTC [GA]^AATT[CT] AgeI 1046 ACCGGT A^CCGGT ApoI 4072 AAATTC [GA]^AATT[CT] BbrPI 3489 CACGTG CAC^GTG BcgI 204 GCAGCATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 2468 AGATCT A^GATCT BsaAI 3489 CACGTG [CT]AC^GT[GA] BsiEI 88 CGGCCG CG[GA][CT]^CG BsrDI 948 GCAATG GCAATG(2/0) DraIII 827 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 88 CGGCCG C^GGCCG EclXI 88 CGGCCG C^GGCCG Eco52I 88 CGGCCG C^GGCCG EcoT22I 2233 ATGCAT ATGCA^T NaeI 677 GCCGGC GCC^GGC NcoI 1266 CCATGG C^CATGG NgoMIV 677 GCCGGC G^CCGGC NheI 1858 GCTAGC G^CTAGC NruI 2379 TCGCGA TCG^CGA NsiI 2233 ATGCAT ATGCA^T PinAI 1046 ACCGGT A^CCGGT PmaCI 3489 CACGTG CAC^GTG PmlI 3489 CACGTG CAC^GTG SapI 1762 GAAGAGC GCTCTTC(1/4) SfiI 4242 GGCCCAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 218 CCCGGG CCC^GGG SspI 3466 AATATT AAT^ATT XbaI 2769 TCTAGA T^CTAGA XmaCI 218 CCCGGG C^CCGGG XmaI 218 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AflIII 3488 ACACGT A^C[GA][CT]GT 3496 ACACGT Asp700I 2557 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3791 GAAGGGCTTC BbeI 2122 GGCGCC GGCGC^C 2828 GGCGCC BsaWI 1046 ACCGGT [AT]^CCGG[AT] 3283 TCCGGT BsgI 482 CTGCAC GTGCAG(16/14) 635 GTGCAG BstAPI 1626 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2262 GCAGAAGCTGC BstEII 282 GGTGACC G^GT[ACGT]ACC 2197 GGTAACC BstPI 282 GGTGACC G^GT[ACGT]ACC 2197 GGTAACC EcoO65I 282 GGTGACC G^GT[ACGT]ACC 2197 GGTAACC HindIII 2560 AAGCTT A^AGCTT 4721 AAGCTT KasI 2122 GGCGCC G^GCGCC 2828 GGCGCC NarI 2122 GGCGCC GG^CGCC 2828 GGCGCC NspI 1910 ACATGC [GA]CATG^[CT] 2744 ACATGC PflMI 3216 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4275 CCAGAAGGTGG PshAI 309 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2371 GACCAATGTC SacI 273 GAGCTC GAGCT^C 2353 GAGCTC SbfI 2202 CCTGCAGG CCTGCA^GG 2685 CCTGCAGG SfoI 2122 GGCGCC GGC^GCC 2828 GGCGCC Sse8387I 2202 CCTGCAGG CCTGCA^GG 2685 CCTGCAGG Van91I 3216 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4275 CCAGAAGGTGG XmnI 2557 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3791 GAAGGGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII