##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AhdI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BciVI       
BclI        BfrI        BlnI        BsaBI       BseAI       
BsiWI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrGI       BssSI       Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        DraI        DrdI        
Eam1105I    Eco47III    EcoRI       EcoRV       FbaI        
FseI        FspI        HincII      HindII      HpaI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MunI        NdeI        NotI        NspV        
PacI        PaeR7I      PflFI       PmeI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        ScaI        SfuI        SgrAI       SnaBI       
SpeI        SphI        SspBI       StuI        SwaI        
Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AcsI         4072  AAATTC               [GA]^AATT[CT]
AgeI         1046  ACCGGT               A^CCGGT
ApoI         4072  AAATTC               [GA]^AATT[CT]
BbrPI        3489  CACGTG               CAC^GTG
BcgI          204  GCAGCATCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        2468  AGATCT               A^GATCT
BsaAI        3489  CACGTG               [CT]AC^GT[GA]
BsiEI          88  CGGCCG               CG[GA][CT]^CG
BsrDI         948  GCAATG               GCAATG(2/0)
DraIII        827  CACTCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI           88  CGGCCG               C^GGCCG
EclXI          88  CGGCCG               C^GGCCG
Eco52I         88  CGGCCG               C^GGCCG
EcoT22I      2233  ATGCAT               ATGCA^T
NaeI          677  GCCGGC               GCC^GGC
NcoI         1266  CCATGG               C^CATGG
NgoMIV        677  GCCGGC               G^CCGGC
NheI         1858  GCTAGC               G^CTAGC
NruI         2379  TCGCGA               TCG^CGA
NsiI         2233  ATGCAT               ATGCA^T
PinAI        1046  ACCGGT               A^CCGGT
PmaCI        3489  CACGTG               CAC^GTG
PmlI         3489  CACGTG               CAC^GTG
SapI         1762  GAAGAGC              GCTCTTC(1/4)
SfiI         4242  GGCCCAGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          218  CCCGGG               CCC^GGG
SspI         3466  AATATT               AAT^ATT
XbaI         2769  TCTAGA               T^CTAGA
XmaCI         218  CCCGGG               C^CCGGG
XmaI          218  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AflIII       3488  ACACGT               A^C[GA][CT]GT
             3496  ACACGT              
Asp700I      2557  GAAAAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3791  GAAGGGCTTC          
BbeI         2122  GGCGCC               GGCGC^C
             2828  GGCGCC              
BsaWI        1046  ACCGGT               [AT]^CCGG[AT]
             3283  TCCGGT              
BsgI          482  CTGCAC               GTGCAG(16/14)
              635  GTGCAG              
BstAPI       1626  GCAGAGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2262  GCAGAAGCTGC         
BstEII        282  GGTGACC              G^GT[ACGT]ACC
             2197  GGTAACC             
BstPI         282  GGTGACC              G^GT[ACGT]ACC
             2197  GGTAACC             
EcoO65I       282  GGTGACC              G^GT[ACGT]ACC
             2197  GGTAACC             
HindIII      2560  AAGCTT               A^AGCTT
             4721  AAGCTT              
KasI         2122  GGCGCC               G^GCGCC
             2828  GGCGCC              
NarI         2122  GGCGCC               GG^CGCC
             2828  GGCGCC              
NspI         1910  ACATGC               [GA]CATG^[CT]
             2744  ACATGC              
PflMI        3216  CCAGCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4275  CCAGAAGGTGG         
PshAI         309  GACCATTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2371  GACCAATGTC          
SacI          273  GAGCTC               GAGCT^C
             2353  GAGCTC              
SbfI         2202  CCTGCAGG             CCTGCA^GG
             2685  CCTGCAGG            
SfoI         2122  GGCGCC               GGC^GCC
             2828  GGCGCC              
Sse8387I     2202  CCTGCAGG             CCTGCA^GG
             2685  CCTGCAGG            
Van91I       3216  CCAGCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4275  CCAGAAGGTGG         
XmnI         2557  GAAAAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3791  GAAGGGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  125 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        ApaI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvCI       BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssHII      BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SexAI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII