##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BciVI
BclI BfrI BlnI BsaBI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
Eam1105I Eco47III EcoRI EcoRV FbaI
FseI FspI HincII HindII HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NdeI NotI NspV
PacI PaeR7I PflFI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SfuI SgrAI SnaBI
SpeI SphI SspBI StuI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 4072 AAATTC [GA]^AATT[CT]
AgeI 1046 ACCGGT A^CCGGT
ApoI 4072 AAATTC [GA]^AATT[CT]
BbrPI 3489 CACGTG CAC^GTG
BcgI 204 GCAGCATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2468 AGATCT A^GATCT
BsaAI 3489 CACGTG [CT]AC^GT[GA]
BsiEI 88 CGGCCG CG[GA][CT]^CG
BsrDI 948 GCAATG GCAATG(2/0)
DraIII 827 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 88 CGGCCG C^GGCCG
EclXI 88 CGGCCG C^GGCCG
Eco52I 88 CGGCCG C^GGCCG
EcoT22I 2233 ATGCAT ATGCA^T
NaeI 677 GCCGGC GCC^GGC
NcoI 1266 CCATGG C^CATGG
NgoMIV 677 GCCGGC G^CCGGC
NheI 1858 GCTAGC G^CTAGC
NruI 2379 TCGCGA TCG^CGA
NsiI 2233 ATGCAT ATGCA^T
PinAI 1046 ACCGGT A^CCGGT
PmaCI 3489 CACGTG CAC^GTG
PmlI 3489 CACGTG CAC^GTG
SapI 1762 GAAGAGC GCTCTTC(1/4)
SfiI 4242 GGCCCAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 218 CCCGGG CCC^GGG
SspI 3466 AATATT AAT^ATT
XbaI 2769 TCTAGA T^CTAGA
XmaCI 218 CCCGGG C^CCGGG
XmaI 218 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 3488 ACACGT A^C[GA][CT]GT
3496 ACACGT
Asp700I 2557 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3791 GAAGGGCTTC
BbeI 2122 GGCGCC GGCGC^C
2828 GGCGCC
BsaWI 1046 ACCGGT [AT]^CCGG[AT]
3283 TCCGGT
BsgI 482 CTGCAC GTGCAG(16/14)
635 GTGCAG
BstAPI 1626 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2262 GCAGAAGCTGC
BstEII 282 GGTGACC G^GT[ACGT]ACC
2197 GGTAACC
BstPI 282 GGTGACC G^GT[ACGT]ACC
2197 GGTAACC
EcoO65I 282 GGTGACC G^GT[ACGT]ACC
2197 GGTAACC
HindIII 2560 AAGCTT A^AGCTT
4721 AAGCTT
KasI 2122 GGCGCC G^GCGCC
2828 GGCGCC
NarI 2122 GGCGCC GG^CGCC
2828 GGCGCC
NspI 1910 ACATGC [GA]CATG^[CT]
2744 ACATGC
PflMI 3216 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4275 CCAGAAGGTGG
PshAI 309 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2371 GACCAATGTC
SacI 273 GAGCTC GAGCT^C
2353 GAGCTC
SbfI 2202 CCTGCAGG CCTGCA^GG
2685 CCTGCAGG
SfoI 2122 GGCGCC GGC^GCC
2828 GGCGCC
Sse8387I 2202 CCTGCAGG CCTGCA^GG
2685 CCTGCAGG
Van91I 3216 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4275 CCAGAAGGTGG
XmnI 2557 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3791 GAAGGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII