##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AccII AccIII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I AspEI AspI BbeI BbrPI
BcgI BfrI BglII BsaAI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BspMI BssHII BstBI BstEII BstPI
BstUI ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FseI HpaI KasI KspI
MfeI MluI MroI MunI MvnI
NarI NdeI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SwaI
Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 1096 GACGTC GACGT^C
Acc65I 1031 GGTACC G^GTACC
AcyI 1096 GACGTC G[GA]^CG[CT]C
AocI 3147 CCTCAGG CC^T[ACGT]AGG
Asp718I 1031 GGTACC G^GTACC
AvaI 1484 CCCGGG C^[CT]CG[GA]G
AviII 3290 TGCGCA TGC^GCA
AvrII 2472 CCTAGG C^CTAGG
BamHI 3769 GGATCC G^GATCC
BciVI 2624 GGATAC GTATCC(6/5)
BlnI 2472 CCTAGG C^CTAGG
BsaBI 4194 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1096 GACGTC G[GA]^CG[CT]C
BsmBI 1688 CGTCTC CGTCTC(1/5)
BsmI 449 GCATTC GAATGC(1/-1)
BsoBI 1484 CCCGGG C^[CT]CG[GA]G
Bsp1407I 945 TGTACA T^GTACA
BspHI 563 TCATGA T^CATGA
BspLU11I 758 ACATGT A^CATGT
BsrDI 115 CATTGC GCAATG(2/0)
BsrGI 945 TGTACA T^GTACA
BssSI 86 CACGAG CACGAG(-5/-1)
Bst1107I 2792 GTATAC GTA^TAC
BstZ17I 2792 GTATAC GTA^TAC
Bsu36I 3147 CCTCAGG CC^T[ACGT]AGG
DraI 4606 TTTAAA TTT^AAA
DraIII 1501 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 3147 CCTCAGG CC^T[ACGT]AGG
EcoRI 3541 GAATTC G^AATTC
FspI 3290 TGCGCA TGC^GCA
HgaI 732 GACGC GACGC(5/10)
Hin1I 1096 GACGTC G[GA]^CG[CT]C
HindIII 879 AAGCTT A^AGCTT
KpnI 1031 GGTACC GGTAC^C
MamI 4194 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2237 GCCGGC GCC^GGC
NgoMIV 2237 GCCGGC G^CCGGC
RcaI 563 TCATGA T^CATGA
SbfI 2682 CCTGCAGG CCTGCA^GG
SmaI 1484 CCCGGG CCC^GGG
Sse8387I 2682 CCTGCAGG CCTGCA^GG
SspBI 945 TGTACA T^GTACA
XbaI 3327 TCTAGA T^CTAGA
XmaCI 1484 CCCGGG C^CCGGG
XmaI 1484 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 1156 GTAGAC GT^[AC][GT]AC
2792 GTATAC
AflIII 758 ACATGT A^C[GA][CT]GT
942 ACGTGT
Alw44I 526 GTGCAC G^TGCAC
2804 GTGCAC
ApaI 2582 GGGCCC GGGCC^C
3152 GGGCCC
ApaLI 526 GTGCAC G^TGCAC
2804 GTGCAC
BbvCI 1178 CCTCAGC CCTCAGC(-5/-2)
2968 GCTGAGG
BsaI 1717 GAGACC GGTCTC(1/5)
1790 GAGACC
BsrFI 2237 GCCGGC [GA]^CCGG[CT]
4466 ACCGGC
BstAPI 1773 GCAACTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1998 GCAGGCCTTGC
Cfr10I 2237 GCCGGC [GA]^CCGG[CT]
4466 ACCGGC
DrdI 1730 GACAGGTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3583 GACATGGAAGTC
FauI 1845 CCCGC CCCGC(4/6)
3615 GCGGG
HaeII 4 GGCGCT [GA]GCGC^[CT]
1271 AGCGCC
NspI 758 ACATGT [GA]CATG^[CT]
2736 ACATGC
SapI 1531 GAAGAGC GCTCTTC(1/4)
4527 GCTCTTC
SspI 3377 AATATT AAT^ATT
4610 AATATT
StuI 1139 AGGCCT AGG^CCT
2000 AGGCCT
XcmI 2456 CCAAGCTCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3221 CCAAGGCGGCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BanI BanII BbsI BbvI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NheI NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII