##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AflII AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspI AvrII BanI BbeI BbrPI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaI BsiWI BspDI BspMI BsrDI
BssHII Bst1107I BstZ17I CelII ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoRI EcoRV FseI HpaI KasI
KpnI KspI MfeI MluI MunI
NarI NdeI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 3370 TCCGGA T^CCGGA
AclI 3400 AACGTT AA^CGTT
AflIII 4433 ACATGT A^C[GA][CT]GT
AhdI 1899 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2476 CCTTAGG CC^T[ACGT]AGG
ApaI 1591 GGGCCC GGGCC^C
AspEI 1899 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 3225 CCCGAG C^[CT]CG[GA]G
BamHI 656 GGATCC G^GATCC
BbvCI 1108 CCTCAGC CCTCAGC(-5/-2)
BciVI 2685 GTATCC GTATCC(6/5)
BclI 2969 TGATCA T^GATCA
BsaWI 3370 TCCGGA [AT]^CCGG[AT]
BseAI 3370 TCCGGA T^CCGGA
BsiEI 74 CGACCG CG[GA][CT]^CG
BsoBI 3225 CCCGAG C^[CT]CG[GA]G
BspEI 3370 TCCGGA T^CCGGA
BspLU11I 4433 ACATGT A^CATGT
BsrBI 82 GAGCGG CCGCTC(-3/-3)
BsrFI 1604 GCCGGC [GA]^CCGG[CT]
BssSI 332 CTCGTG CACGAG(-5/-1)
BstAPI 2937 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 963 TTCGAA TT^CGAA
BstEII 4722 GGTGACC G^GT[ACGT]ACC
BstPI 4722 GGTGACC G^GT[ACGT]ACC
BstXI 378 CCAGTCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2476 CCTTAGG CC^T[ACGT]AGG
Cfr10I 1604 GCCGGC [GA]^CCGG[CT]
Eam1105I 1899 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2476 CCTTAGG CC^T[ACGT]AGG
EcoO65I 4722 GGTGACC G^GT[ACGT]ACC
EcoT22I 439 ATGCAT ATGCA^T
FbaI 2969 TGATCA T^GATCA
HaeII 3688 AGCGCC [GA]GCGC^[CT]
HincII 4159 GTTGAC GT[CT]^[GA]AC
HindII 4159 GTTGAC GT[CT]^[GA]AC
HindIII 2577 AAGCTT A^AGCTT
MroI 3370 TCCGGA T^CCGGA
MslI 752 CATTAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1604 GCCGGC GCC^GGC
NcoI 397 CCATGG C^CATGG
NgoMIV 1604 GCCGGC G^CCGGC
NheI 3875 GCTAGC G^CTAGC
NsiI 439 ATGCAT ATGCA^T
NspV 963 TTCGAA TT^CGAA
Psp1406I 3400 AACGTT AA^CGTT
SapI 4083 GCTCTTC GCTCTTC(1/4)
SfuI 963 TTCGAA TT^CGAA
SpeI 3941 ACTAGT A^CTAGT
StuI 3331 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 795 GACGTC GACGT^C
2989 GACGTC
AccI 1412 GTCTAC GT^[AC][GT]AC
1440 GTAGAC
Asp700I 3153 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4288 GAAATCATTC
AviII 3311 TGCGCA TGC^GCA
4320 TGCGCA
BalI 1183 TGGCCA TGG^CCA
4060 TGGCCA
BcgI 1952 CGATGGATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2869 CGAGAGTTCTGC
BcnI 1808 CCGGG CC^[GC]GG
3857 CCGGG
BglI 1599 GCCCCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2229 GCCCGTGGGGC
BsaBI 1068 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4419 GATGAGAATC
BsgI 1784 GTGCAG GTGCAG(16/14)
4751 GTGCAG
BsmBI 2987 GAGACG CGTCTC(1/5)
3964 GAGACG
BspHI 4284 TCATGA T^CATGA
4506 TCATGA
DsaI 397 CCATGG C^C[GA][CT]GG
2231 CCGTGG
EaeI 1183 TGGCCA [CT]^GGCC[GA]
4060 TGGCCA
EcoNI 872 CCTTCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3781 CCTTAATAAGG
FspI 3311 TGCGCA TGC^GCA
4320 TGCGCA
MamI 1068 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4419 GATGAGAATC
MluNI 1183 TGGCCA TGG^CCA
4060 TGGCCA
MscI 1183 TGGCCA TGG^CCA
4060 TGGCCA
NciI 1808 CCGGG CC^[GC]GG
3857 CCGGG
PpuMI 3979 GGGACCT [GA]G^G[AT]CC[CT]
4134 AGGTCCC
PshAI 692 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3603 GACACCAGTC
RcaI 4284 TCATGA T^CATGA
4506 TCATGA
SacI 2402 GAGCTC GAGCT^C
4249 GAGCTC
ScaI 311 AGTACT AGT^ACT
2448 AGTACT
SspI 3132 AATATT AAT^ATT
4879 AATATT
XmnI 3153 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4288 GAAATCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BanII BbsI BbvI BfaI
BglII BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DrdI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII