################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## Acc65I AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspI AvrII BanI BbeI BbrPI BfrI BlnI BlpI Bpu1102I BsaAI BsaI BsiWI BspDI BspMI BsrDI BssHII Bst1107I BstZ17I CelII ClaI CpoI EagI EclXI Eco47III Eco52I EcoRI EcoRV FseI HpaI KasI KpnI KspI MfeI MluI MunI NarI NdeI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccIII 3370 TCCGGA T^CCGGA AclI 3400 AACGTT AA^CGTT AflIII 4433 ACATGT A^C[GA][CT]GT AhdI 1899 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2476 CCTTAGG CC^T[ACGT]AGG ApaI 1591 GGGCCC GGGCC^C AspEI 1899 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 3225 CCCGAG C^[CT]CG[GA]G BamHI 656 GGATCC G^GATCC BbvCI 1108 CCTCAGC CCTCAGC(-5/-2) BciVI 2685 GTATCC GTATCC(6/5) BclI 2969 TGATCA T^GATCA BsaWI 3370 TCCGGA [AT]^CCGG[AT] BseAI 3370 TCCGGA T^CCGGA BsiEI 74 CGACCG CG[GA][CT]^CG BsoBI 3225 CCCGAG C^[CT]CG[GA]G BspEI 3370 TCCGGA T^CCGGA BspLU11I 4433 ACATGT A^CATGT BsrBI 82 GAGCGG CCGCTC(-3/-3) BsrFI 1604 GCCGGC [GA]^CCGG[CT] BssSI 332 CTCGTG CACGAG(-5/-1) BstAPI 2937 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 963 TTCGAA TT^CGAA BstEII 4722 GGTGACC G^GT[ACGT]ACC BstPI 4722 GGTGACC G^GT[ACGT]ACC BstXI 378 CCAGTCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2476 CCTTAGG CC^T[ACGT]AGG Cfr10I 1604 GCCGGC [GA]^CCGG[CT] Eam1105I 1899 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2476 CCTTAGG CC^T[ACGT]AGG EcoO65I 4722 GGTGACC G^GT[ACGT]ACC EcoT22I 439 ATGCAT ATGCA^T FbaI 2969 TGATCA T^GATCA HaeII 3688 AGCGCC [GA]GCGC^[CT] HincII 4159 GTTGAC GT[CT]^[GA]AC HindII 4159 GTTGAC GT[CT]^[GA]AC HindIII 2577 AAGCTT A^AGCTT MroI 3370 TCCGGA T^CCGGA MslI 752 CATTAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 1604 GCCGGC GCC^GGC NcoI 397 CCATGG C^CATGG NgoMIV 1604 GCCGGC G^CCGGC NheI 3875 GCTAGC G^CTAGC NsiI 439 ATGCAT ATGCA^T NspV 963 TTCGAA TT^CGAA Psp1406I 3400 AACGTT AA^CGTT SapI 4083 GCTCTTC GCTCTTC(1/4) SfuI 963 TTCGAA TT^CGAA SpeI 3941 ACTAGT A^CTAGT StuI 3331 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AatII 795 GACGTC GACGT^C 2989 GACGTC AccI 1412 GTCTAC GT^[AC][GT]AC 1440 GTAGAC Asp700I 3153 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4288 GAAATCATTC AviII 3311 TGCGCA TGC^GCA 4320 TGCGCA BalI 1183 TGGCCA TGG^CCA 4060 TGGCCA BcgI 1952 CGATGGATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2869 CGAGAGTTCTGC BcnI 1808 CCGGG CC^[GC]GG 3857 CCGGG BglI 1599 GCCCCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2229 GCCCGTGGGGC BsaBI 1068 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4419 GATGAGAATC BsgI 1784 GTGCAG GTGCAG(16/14) 4751 GTGCAG BsmBI 2987 GAGACG CGTCTC(1/5) 3964 GAGACG BspHI 4284 TCATGA T^CATGA 4506 TCATGA DsaI 397 CCATGG C^C[GA][CT]GG 2231 CCGTGG EaeI 1183 TGGCCA [CT]^GGCC[GA] 4060 TGGCCA EcoNI 872 CCTTCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3781 CCTTAATAAGG FspI 3311 TGCGCA TGC^GCA 4320 TGCGCA MamI 1068 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4419 GATGAGAATC MluNI 1183 TGGCCA TGG^CCA 4060 TGGCCA MscI 1183 TGGCCA TGG^CCA 4060 TGGCCA NciI 1808 CCGGG CC^[GC]GG 3857 CCGGG PpuMI 3979 GGGACCT [GA]G^G[AT]CC[CT] 4134 AGGTCCC PshAI 692 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3603 GACACCAGTC RcaI 4284 TCATGA T^CATGA 4506 TCATGA SacI 2402 GAGCTC GAGCT^C 4249 GAGCTC ScaI 311 AGTACT AGT^ACT 2448 AGTACT SspI 3132 AATATT AAT^ATT 4879 AATATT XmnI 3153 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4288 GAAATCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanII BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DrdI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII