##################################
# NO CUT ENZYMES (   76 enzymes) #
##################################

Acc65I      AccII       AflII       AgeI        AhdI        
Aor51HI     AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       BamHI       
BfrI        BsiEI       BsiWI       Bsp1407I    BspDI       
BsrFI       BsrGI       BssHII      Bst1107I    BstBI       
BstUI       BstZ17I     Cfr10I      ClaI        CpoI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRV       FseI        FspI        
HpaI        KpnI        KspI        MluI        MvnI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshAI       PshBI       PvuI        
RsrII       SacII       SalI        SbfI        SfuI        
SgrAI       SmaI        SnaBI       Sse8387I    SspBI       
SwaI        Tth111I     XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII         422  GACGTC               GACGT^C
AccI         3933  GTCTAC               GT^[AC][GT]AC
AccIII       3331  TCCGGA               T^CCGGA
AclI          813  AACGTT               AA^CGTT
Alw44I       3879  GTGCAC               G^TGCAC
ApaLI        3879  GTGCAC               G^TGCAC
AvaI         2675  CCCGAG               C^[CT]CG[GA]G
BbeI         2647  GGCGCC               GGCGC^C
BbrPI        2619  CACGTG               CAC^GTG
BclI         2296  TGATCA               T^GATCA
BsaAI        2619  CACGTG               [CT]AC^GT[GA]
BsaBI        3945  GATGAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        3331  TCCGGA               T^CCGGA
BsmI         3378  GCATTC               GAATGC(1/-1)
BsoBI        2675  CCCGAG               C^[CT]CG[GA]G
BspEI        3331  TCCGGA               T^CCGGA
BstEII        377  GGTTACC              G^GT[ACGT]ACC
BstPI         377  GGTTACC              G^GT[ACGT]ACC
CfoI         2648  GCGC                 GCG^C
EcoO65I       377  GGTTACC              G^GT[ACGT]ACC
EcoRI        4845  GAATTC               G^AATTC
FbaI         2296  TGATCA               T^GATCA
HaeII        2647  GGCGCC               [GA]GCGC^[CT]
HhaI         2648  GCGC                 GCG^C
HinP1I       2648  GCGC                 G^CGC
HincII        737  GTCAAC               GT[CT]^[GA]AC
HindII        737  GTCAAC               GT[CT]^[GA]AC
HindIII       268  AAGCTT               A^AGCTT
KasI         2647  GGCGCC               G^GCGCC
MamI         3945  GATGAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         4355  CAATTG               C^AATTG
MroI         3331  TCCGGA               T^CCGGA
MunI         4355  CAATTG               C^AATTG
NarI         2647  GGCGCC               GG^CGCC
PmaCI        2619  CACGTG               CAC^GTG
PmlI         2619  CACGTG               CAC^GTG
Psp1406I      813  AACGTT               AA^CGTT
SexAI         327  ACCTGGT              A^CC[AT]GGT
SfoI         2647  GGCGCC               GGC^GCC
SpeI           74  ACTAGT               A^CTAGT
SspI         3230  AATATT               AAT^ATT
XbaI         2206  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AlwNI         836  CAGTCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             4142  CAGGTGCTG           
AocI         2246  CCTGAGG              CC^T[ACGT]AGG
             5038  CCTCAGG             
ApaI         1351  GGGCCC               GGGCC^C
             2839  GGGCCC              
AvrII         886  CCTAGG               C^CTAGG
             3798  CCTAGG              
BanI         2647  GGCGCC               G^G[CT][GA]CC
             4555  GGCACC              
BcgI         2032  GCATAAACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             4977  GCAATGGACTCG        
BlnI          886  CCTAGG               C^CTAGG
             3798  CCTAGG              
BspHI         892  TCATGA               T^CATGA
             1873  TCATGA              
BspLU11I      238  ACATGT               A^CATGT
             3300  ACATGT              
BspMI        1330  ACCTGC               ACCTGC(4/8)
             1297  GCAGGT              
BsrBI        1305  GAGCGG               CCGCTC(-3/-3)
             2327  GAGCGG              
BssSI        2815  CTCGTG               CACGAG(-5/-1)
             5067  CTCGTG              
BstAPI       2930  GCAACCTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4024  GCAGCAGATGC         
Bsu36I       2246  CCTGAGG              CC^T[ACGT]AGG
             5038  CCTCAGG             
Eco81I       2246  CCTGAGG              CC^T[ACGT]AGG
             5038  CCTCAGG             
EcoT22I      1562  ATGCAT               ATGCA^T
             2030  ATGCAT              
NsiI         1562  ATGCAT               ATGCA^T
             2030  ATGCAT              
PflMI        1676  CCACCTTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4141  CCAGGTGCTGG         
RcaI          892  TCATGA               T^CATGA
             1873  TCATGA              
SacI          124  GAGCTC               GAGCT^C
             2793  GAGCTC              
ScaI          252  AGTACT               AGT^ACT
             1143  AGTACT              
SfiI         1343  GGCCATCTGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1723  GGCCAACTTGGCC       
SphI         2028  GCATGC               GCATG^C
             3793  GCATGC              
Van91I       1676  CCACCTTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4141  CCAGGTGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  116 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AflIII      
AluI        AlwI        ApoI        AspHI       AvaII       
BalI        BanII       BbsI        BbvCI       BbvI        
BciVI       BcnI        BfaI        BglI        BglII       
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       Bsp1286I    BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       CelII       Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
Hin1I       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       PstI        
PvuII       RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII