################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## Acc65I AccII AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BamHI BfrI BsiEI BsiWI Bsp1407I BspDI BsrFI BsrGI BssHII Bst1107I BstBI BstUI BstZ17I Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FseI FspI HpaI KpnI KspI MluI MvnI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI PvuI RsrII SacII SalI SbfI SfuI SgrAI SmaI SnaBI Sse8387I SspBI SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 422 GACGTC GACGT^C AccI 3933 GTCTAC GT^[AC][GT]AC AccIII 3331 TCCGGA T^CCGGA AclI 813 AACGTT AA^CGTT Alw44I 3879 GTGCAC G^TGCAC ApaLI 3879 GTGCAC G^TGCAC AvaI 2675 CCCGAG C^[CT]CG[GA]G BbeI 2647 GGCGCC GGCGC^C BbrPI 2619 CACGTG CAC^GTG BclI 2296 TGATCA T^GATCA BsaAI 2619 CACGTG [CT]AC^GT[GA] BsaBI 3945 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 3331 TCCGGA T^CCGGA BsmI 3378 GCATTC GAATGC(1/-1) BsoBI 2675 CCCGAG C^[CT]CG[GA]G BspEI 3331 TCCGGA T^CCGGA BstEII 377 GGTTACC G^GT[ACGT]ACC BstPI 377 GGTTACC G^GT[ACGT]ACC CfoI 2648 GCGC GCG^C EcoO65I 377 GGTTACC G^GT[ACGT]ACC EcoRI 4845 GAATTC G^AATTC FbaI 2296 TGATCA T^GATCA HaeII 2647 GGCGCC [GA]GCGC^[CT] HhaI 2648 GCGC GCG^C HinP1I 2648 GCGC G^CGC HincII 737 GTCAAC GT[CT]^[GA]AC HindII 737 GTCAAC GT[CT]^[GA]AC HindIII 268 AAGCTT A^AGCTT KasI 2647 GGCGCC G^GCGCC MamI 3945 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 4355 CAATTG C^AATTG MroI 3331 TCCGGA T^CCGGA MunI 4355 CAATTG C^AATTG NarI 2647 GGCGCC GG^CGCC PmaCI 2619 CACGTG CAC^GTG PmlI 2619 CACGTG CAC^GTG Psp1406I 813 AACGTT AA^CGTT SexAI 327 ACCTGGT A^CC[AT]GGT SfoI 2647 GGCGCC GGC^GCC SpeI 74 ACTAGT A^CTAGT SspI 3230 AATATT AAT^ATT XbaI 2206 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwNI 836 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG 4142 CAGGTGCTG AocI 2246 CCTGAGG CC^T[ACGT]AGG 5038 CCTCAGG ApaI 1351 GGGCCC GGGCC^C 2839 GGGCCC AvrII 886 CCTAGG C^CTAGG 3798 CCTAGG BanI 2647 GGCGCC G^G[CT][GA]CC 4555 GGCACC BcgI 2032 GCATAAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4977 GCAATGGACTCG BlnI 886 CCTAGG C^CTAGG 3798 CCTAGG BspHI 892 TCATGA T^CATGA 1873 TCATGA BspLU11I 238 ACATGT A^CATGT 3300 ACATGT BspMI 1330 ACCTGC ACCTGC(4/8) 1297 GCAGGT BsrBI 1305 GAGCGG CCGCTC(-3/-3) 2327 GAGCGG BssSI 2815 CTCGTG CACGAG(-5/-1) 5067 CTCGTG BstAPI 2930 GCAACCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4024 GCAGCAGATGC Bsu36I 2246 CCTGAGG CC^T[ACGT]AGG 5038 CCTCAGG Eco81I 2246 CCTGAGG CC^T[ACGT]AGG 5038 CCTCAGG EcoT22I 1562 ATGCAT ATGCA^T 2030 ATGCAT NsiI 1562 ATGCAT ATGCA^T 2030 ATGCAT PflMI 1676 CCACCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4141 CCAGGTGCTGG RcaI 892 TCATGA T^CATGA 1873 TCATGA SacI 124 GAGCTC GAGCT^C 2793 GAGCTC ScaI 252 AGTACT AGT^ACT 1143 AGTACT SfiI 1343 GGCCATCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1723 GGCCAACTTGGCC SphI 2028 GCATGC GCATG^C 3793 GCATGC Van91I 1676 CCACCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4141 CCAGGTGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI AlwI ApoI AspHI AvaII BalI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI Hin1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII