##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccII AflII AgeI AhdI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII BamHI
BfrI BsiEI BsiWI Bsp1407I BspDI
BsrFI BsrGI BssHII Bst1107I BstBI
BstUI BstZ17I Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FseI FspI
HpaI KpnI KspI MluI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI PvuI
RsrII SacII SalI SbfI SfuI
SgrAI SmaI SnaBI Sse8387I SspBI
SwaI Tth111I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 422 GACGTC GACGT^C
AccI 3933 GTCTAC GT^[AC][GT]AC
AccIII 3331 TCCGGA T^CCGGA
AclI 813 AACGTT AA^CGTT
Alw44I 3879 GTGCAC G^TGCAC
ApaLI 3879 GTGCAC G^TGCAC
AvaI 2675 CCCGAG C^[CT]CG[GA]G
BbeI 2647 GGCGCC GGCGC^C
BbrPI 2619 CACGTG CAC^GTG
BclI 2296 TGATCA T^GATCA
BsaAI 2619 CACGTG [CT]AC^GT[GA]
BsaBI 3945 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3331 TCCGGA T^CCGGA
BsmI 3378 GCATTC GAATGC(1/-1)
BsoBI 2675 CCCGAG C^[CT]CG[GA]G
BspEI 3331 TCCGGA T^CCGGA
BstEII 377 GGTTACC G^GT[ACGT]ACC
BstPI 377 GGTTACC G^GT[ACGT]ACC
CfoI 2648 GCGC GCG^C
EcoO65I 377 GGTTACC G^GT[ACGT]ACC
EcoRI 4845 GAATTC G^AATTC
FbaI 2296 TGATCA T^GATCA
HaeII 2647 GGCGCC [GA]GCGC^[CT]
HhaI 2648 GCGC GCG^C
HinP1I 2648 GCGC G^CGC
HincII 737 GTCAAC GT[CT]^[GA]AC
HindII 737 GTCAAC GT[CT]^[GA]AC
HindIII 268 AAGCTT A^AGCTT
KasI 2647 GGCGCC G^GCGCC
MamI 3945 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4355 CAATTG C^AATTG
MroI 3331 TCCGGA T^CCGGA
MunI 4355 CAATTG C^AATTG
NarI 2647 GGCGCC GG^CGCC
PmaCI 2619 CACGTG CAC^GTG
PmlI 2619 CACGTG CAC^GTG
Psp1406I 813 AACGTT AA^CGTT
SexAI 327 ACCTGGT A^CC[AT]GGT
SfoI 2647 GGCGCC GGC^GCC
SpeI 74 ACTAGT A^CTAGT
SspI 3230 AATATT AAT^ATT
XbaI 2206 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 836 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
4142 CAGGTGCTG
AocI 2246 CCTGAGG CC^T[ACGT]AGG
5038 CCTCAGG
ApaI 1351 GGGCCC GGGCC^C
2839 GGGCCC
AvrII 886 CCTAGG C^CTAGG
3798 CCTAGG
BanI 2647 GGCGCC G^G[CT][GA]CC
4555 GGCACC
BcgI 2032 GCATAAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4977 GCAATGGACTCG
BlnI 886 CCTAGG C^CTAGG
3798 CCTAGG
BspHI 892 TCATGA T^CATGA
1873 TCATGA
BspLU11I 238 ACATGT A^CATGT
3300 ACATGT
BspMI 1330 ACCTGC ACCTGC(4/8)
1297 GCAGGT
BsrBI 1305 GAGCGG CCGCTC(-3/-3)
2327 GAGCGG
BssSI 2815 CTCGTG CACGAG(-5/-1)
5067 CTCGTG
BstAPI 2930 GCAACCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4024 GCAGCAGATGC
Bsu36I 2246 CCTGAGG CC^T[ACGT]AGG
5038 CCTCAGG
Eco81I 2246 CCTGAGG CC^T[ACGT]AGG
5038 CCTCAGG
EcoT22I 1562 ATGCAT ATGCA^T
2030 ATGCAT
NsiI 1562 ATGCAT ATGCA^T
2030 ATGCAT
PflMI 1676 CCACCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4141 CCAGGTGCTGG
RcaI 892 TCATGA T^CATGA
1873 TCATGA
SacI 124 GAGCTC GAGCT^C
2793 GAGCTC
ScaI 252 AGTACT AGT^ACT
1143 AGTACT
SfiI 1343 GGCCATCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1723 GGCCAACTTGGCC
SphI 2028 GCATGC GCATG^C
3793 GCATGC
Van91I 1676 CCACCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4141 CCAGGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI ApoI AspHI AvaII
BalI BanII BbsI BbvCI BbvI
BciVI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
Hin1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII