##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AscI AseI AsnI Asp718I AvrII
BbrPI BciVI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiWI
BsmI BspEI BspHI BsrBI BssHII
CelII CpoI EcoT22I HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MunI NcoI
NdeI NheI NotI NruI NsiI
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SgrAI
SnaBI SwaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AhdI 968 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2120 GTGCAC G^TGCAC
AocI 2418 CCTGAGG CC^T[ACGT]AGG
ApaLI 2120 GTGCAC G^TGCAC
Asp700I 4125 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 968 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 989 TGCGCA TGC^GCA
BbeI 1925 GGCGCC GGCGC^C
BclI 1388 TGATCA T^GATCA
BspDI 1177 ATCGAT AT^CGAT
BsrFI 902 GCCGGC [GA]^CCGG[CT]
Bst1107I 2111 GTATAC GTA^TAC
BstBI 121 TTCGAA TT^CGAA
BstXI 797 CCAAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2111 GTATAC GTA^TAC
Bsu36I 2418 CCTGAGG CC^T[ACGT]AGG
Cfr10I 902 GCCGGC [GA]^CCGG[CT]
ClaI 1177 ATCGAT AT^CGAT
DraI 4206 TTTAAA TTT^AAA
DrdI 2183 GACTGACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 968 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2418 CCTGAGG CC^T[ACGT]AGG
EcoNI 2651 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3 GAATTC G^AATTC
EcoRV 142 GATATC GAT^ATC
EcoT14I 890 CCAAGG C^C[AT][AT]GG
FbaI 1388 TGATCA T^GATCA
FseI 901 GGCCGGCC GGCCGG^CC
FspI 989 TGCGCA TGC^GCA
KasI 1925 GGCGCC G^GCGCC
NaeI 902 GCCGGC GCC^GGC
NarI 1925 GGCGCC GG^CGCC
NgoMIV 902 GCCGGC G^CCGGC
NspV 121 TTCGAA TT^CGAA
PaeR7I 8 CTCGAG C^TCGAG
PflMI 1231 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 3845 GAAGAGC GCTCTTC(1/4)
SbfI 1719 CCTGCAGG CCTGCA^GG
SfoI 1925 GGCGCC GGC^GCC
SfuI 121 TTCGAA TT^CGAA
SmaI 602 CCCGGG CCC^GGG
SphI 3234 GCATGC GCATG^C
Sse8387I 1719 CCTGCAGG CCTGCA^GG
SspI 613 AATATT AAT^ATT
StyI 890 CCAAGG C^C[AT][AT]GG
Van91I 1231 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 8 CTCGAG C^TCGAG
XmaCI 602 CCCGGG C^CCGGG
XmaI 602 CCCGGG C^CCGGG
XmnI 4125 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AcyI 1375 GACGCC G[GA]^CG[CT]C
1925 GGCGCC
AflII 2320 CTTAAG C^TTAAG
3859 CTTAAG
AflIII 316 ACATGT A^C[GA][CT]GT
3790 ACATGT
Aor51HI 3591 AGCGCT AGC^GCT
3770 AGCGCT
ApaI 1152 GGGCCC GGGCC^C
3290 GGGCCC
AspI 1420 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1531 GACAAAGTC
BalI 1627 TGGCCA TGG^CCA
3352 TGGCCA
BamHI 2371 GGATCC G^GATCC
2862 GGATCC
BanI 1925 GGCGCC G^G[CT][GA]CC
3517 GGCACC
BbvCI 2888 CCTCAGC CCTCAGC(-5/-2)
3949 CCTCAGC
BfrI 2320 CTTAAG C^TTAAG
3859 CTTAAG
BglII 2015 AGATCT A^GATCT
3083 AGATCT
BsaHI 1375 GACGCC G[GA]^CG[CT]C
1925 GGCGCC
BsiEI 760 CGGCCG CG[GA][CT]^CG
900 CGGCCG
BsmBI 870 GAGACG CGTCTC(1/5)
1024 GAGACG
Bsp1407I 1259 TGTACA T^GTACA
3597 TGTACA
BspLU11I 316 ACATGT A^CATGT
3790 ACATGT
BsrDI 2531 CATTGC GCAATG(2/0)
2961 GCAATG
BsrGI 1259 TGTACA T^GTACA
3597 TGTACA
BssSI 915 CTCGTG CACGAG(-5/-1)
2498 CTCGTG
BstEII 490 GGTGACC G^GT[ACGT]ACC
4007 GGTGACC
BstPI 490 GGTGACC G^GT[ACGT]ACC
4007 GGTGACC
DraIII 667 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2427 CACCCAGTG
EagI 760 CGGCCG C^GGCCG
900 CGGCCG
EclXI 760 CGGCCG C^GGCCG
900 CGGCCG
Eco47III 3591 AGCGCT AGC^GCT
3770 AGCGCT
Eco52I 760 CGGCCG C^GGCCG
900 CGGCCG
EcoO65I 490 GGTGACC G^GT[ACGT]ACC
4007 GGTGACC
Hin1I 1375 GACGCC G[GA]^CG[CT]C
1925 GGCGCC
MluNI 1627 TGGCCA TGG^CCA
3352 TGGCCA
MscI 1627 TGGCCA TGG^CCA
3352 TGGCCA
PflFI 1420 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1531 GACAAAGTC
SfiI 1153 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3551 GGCCTTTCTGGCC
SpeI 2031 ACTAGT A^CTAGT
2241 ACTAGT
SspBI 1259 TGTACA T^GTACA
3597 TGTACA
StuI 864 AGGCCT AGG^CCT
1161 AGGCCT
Tth111I 1420 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1531 GACAAAGTC
XbaI 2001 TCTAGA T^CTAGA
3254 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanII BbsI BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII