################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BbrPI BfrI BglII BlnI BsaAI BsiWI Bsp1407I BspHI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstZ17I Cfr10I CpoI EagI Eam1105I EclXI Eco52I EcoRI EcoRV EcoT22I FseI HpaI KpnI KspI MaeII MfeI MluI MunI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmlI PshAI PshBI Psp1406I RcaI RsrII SacII SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XcmI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AccII 1906 CGCG CG^CG AflIII 3720 ACATGT A^C[GA][CT]GT BcgI 3461 CGAACCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3940 GGATAC GTATCC(6/5) BclI 525 TGATCA T^GATCA BsaBI 340 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 345 CGATCG CG[GA][CT]^CG BsmBI 2128 GAGACG CGTCTC(1/5) BspLU11I 3720 ACATGT A^CATGT BstUI 1906 CGCG CG^CG BstXI 2645 CCAGGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 4518 TTTAAA TTT^AAA DrdI 2161 GACCTGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 525 TGATCA T^GATCA HincII 1898 GTCGAC GT[CT]^[GA]AC HindII 1898 GTCGAC GT[CT]^[GA]AC HindIII 716 AAGCTT A^AGCTT MamI 340 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 1906 CGCG CG^CG NcoI 3698 CCATGG C^CATGG NdeI 3729 CATATG CA^TATG PmeI 4517 GTTTAAAC GTTT^AAAC PvuI 345 CGATCG CGAT^CG SalI 1898 GTCGAC G^TCGAC SexAI 1622 ACCAGGT A^CC[AT]GGT SmaI 517 CCCGGG CCC^GGG SphI 3897 GCATGC GCATG^C StuI 1446 AGGCCT AGG^CCT XmaCI 517 CCCGGG C^CCGGG XmaI 517 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccIII 215 TCCGGA T^CCGGA 3395 TCCGGA Aor51HI 1465 AGCGCT AGC^GCT 3311 AGCGCT Asp700I 4236 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4372 GAACTGATTC BalI 398 TGGCCA TGG^CCA 2564 TGGCCA BamHI 2773 GGATCC G^GATCC 3830 GGATCC BbeI 2552 GGCGCC GGCGC^C 3429 GGCGCC BseAI 215 TCCGGA T^CCGGA 3395 TCCGGA BspDI 2155 ATCGAT AT^CGAT 3364 ATCGAT BspEI 215 TCCGGA T^CCGGA 3395 TCCGGA BssSI 1693 CACGAG CACGAG(-5/-1) 1837 CACGAG BstEII 1626 GGTGACC G^GT[ACGT]ACC 2790 GGTGACC BstPI 1626 GGTGACC G^GT[ACGT]ACC 2790 GGTGACC ClaI 2155 ATCGAT AT^CGAT 3364 ATCGAT DsaI 678 CCACGG C^C[GA][CT]GG 3698 CCATGG Eco47III 1465 AGCGCT AGC^GCT 3311 AGCGCT EcoNI 2202 CCTGTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2397 CCTGAGTAAGG EcoO65I 1626 GGTGACC G^GT[ACGT]ACC 2790 GGTGACC KasI 2552 GGCGCC G^GCGCC 3429 GGCGCC MluNI 398 TGGCCA TGG^CCA 2564 TGGCCA MroI 215 TCCGGA T^CCGGA 3395 TCCGGA MscI 398 TGGCCA TGG^CCA 2564 TGGCCA NarI 2552 GGCGCC GG^CGCC 3429 GGCGCC SapI 13 GCTCTTC GCTCTTC(1/4) 4166 GCTCTTC SfoI 2552 GGCGCC GGC^GCC 3429 GGCGCC XmnI 4236 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4372 GAACTGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccI AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII AviII BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PaeR7I PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoI XhoII