##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI Alw44I ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BbrPI BfrI BglII
BlnI BsaAI BsiWI Bsp1407I BspHI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstZ17I Cfr10I CpoI EagI
Eam1105I EclXI Eco52I EcoRI EcoRV
EcoT22I FseI HpaI KpnI KspI
MaeII MfeI MluI MunI NaeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmlI PshAI PshBI Psp1406I RcaI
RsrII SacII SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XcmI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccII 1906 CGCG CG^CG
AflIII 3720 ACATGT A^C[GA][CT]GT
BcgI 3461 CGAACCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3940 GGATAC GTATCC(6/5)
BclI 525 TGATCA T^GATCA
BsaBI 340 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 345 CGATCG CG[GA][CT]^CG
BsmBI 2128 GAGACG CGTCTC(1/5)
BspLU11I 3720 ACATGT A^CATGT
BstUI 1906 CGCG CG^CG
BstXI 2645 CCAGGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 4518 TTTAAA TTT^AAA
DrdI 2161 GACCTGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 525 TGATCA T^GATCA
HincII 1898 GTCGAC GT[CT]^[GA]AC
HindII 1898 GTCGAC GT[CT]^[GA]AC
HindIII 716 AAGCTT A^AGCTT
MamI 340 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1906 CGCG CG^CG
NcoI 3698 CCATGG C^CATGG
NdeI 3729 CATATG CA^TATG
PmeI 4517 GTTTAAAC GTTT^AAAC
PvuI 345 CGATCG CGAT^CG
SalI 1898 GTCGAC G^TCGAC
SexAI 1622 ACCAGGT A^CC[AT]GGT
SmaI 517 CCCGGG CCC^GGG
SphI 3897 GCATGC GCATG^C
StuI 1446 AGGCCT AGG^CCT
XmaCI 517 CCCGGG C^CCGGG
XmaI 517 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 215 TCCGGA T^CCGGA
3395 TCCGGA
Aor51HI 1465 AGCGCT AGC^GCT
3311 AGCGCT
Asp700I 4236 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4372 GAACTGATTC
BalI 398 TGGCCA TGG^CCA
2564 TGGCCA
BamHI 2773 GGATCC G^GATCC
3830 GGATCC
BbeI 2552 GGCGCC GGCGC^C
3429 GGCGCC
BseAI 215 TCCGGA T^CCGGA
3395 TCCGGA
BspDI 2155 ATCGAT AT^CGAT
3364 ATCGAT
BspEI 215 TCCGGA T^CCGGA
3395 TCCGGA
BssSI 1693 CACGAG CACGAG(-5/-1)
1837 CACGAG
BstEII 1626 GGTGACC G^GT[ACGT]ACC
2790 GGTGACC
BstPI 1626 GGTGACC G^GT[ACGT]ACC
2790 GGTGACC
ClaI 2155 ATCGAT AT^CGAT
3364 ATCGAT
DsaI 678 CCACGG C^C[GA][CT]GG
3698 CCATGG
Eco47III 1465 AGCGCT AGC^GCT
3311 AGCGCT
EcoNI 2202 CCTGTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2397 CCTGAGTAAGG
EcoO65I 1626 GGTGACC G^GT[ACGT]ACC
2790 GGTGACC
KasI 2552 GGCGCC G^GCGCC
3429 GGCGCC
MluNI 398 TGGCCA TGG^CCA
2564 TGGCCA
MroI 215 TCCGGA T^CCGGA
3395 TCCGGA
MscI 398 TGGCCA TGG^CCA
2564 TGGCCA
NarI 2552 GGCGCC GG^CGCC
3429 GGCGCC
SapI 13 GCTCTTC GCTCTTC(1/4)
4166 GCTCTTC
SfoI 2552 GGCGCC GGC^GCC
3429 GGCGCC
XmnI 4236 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4372 GAACTGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII AviII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PaeR7I PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoI XhoII