##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AclI AflII ApaI AscI
AseI AsnI Asp700I BalI BbeI
BbvCI BclI BfrI BglI BsgI
BsiWI BsmBI BspMI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CpoI EagI EclXI Eco52I EcoO65I
EcoRV FbaI FseI HpaI KasI
MluI MluNI MscI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 3191 GGTACC G^GTACC
AccIII 2558 TCCGGA T^CCGGA
AflIII 2669 ACATGT A^C[GA][CT]GT
AgeI 1356 ACCGGT A^CCGGT
AhdI 3584 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 3191 GGTACC G^GTACC
AspEI 3584 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 996 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2581 TGCGCA TGC^GCA
AvrII 3722 CCTAGG C^CTAGG
BbrPI 2770 CACGTG CAC^GTG
BcgI 1839 CGACACCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 359 GTATCC GTATCC(6/5)
BglII 1986 AGATCT A^GATCT
BlnI 3722 CCTAGG C^CTAGG
BlpI 1712 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1712 GCTTAGC GC^T[ACGT]AGC
BsaAI 2770 CACGTG [CT]AC^GT[GA]
BsaBI 130 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1696 GAGACC GGTCTC(1/5)
BseAI 2558 TCCGGA T^CCGGA
BsiEI 42 CGGTCG CG[GA][CT]^CG
BspEI 2558 TCCGGA T^CCGGA
BspHI 2810 TCATGA T^CATGA
BspLU11I 2669 ACATGT A^CATGT
BstAPI 253 GCAAACGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1712 GCTTAGC GC^T[ACGT]AGC
DraIII 1754 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2908 GACTTAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3584 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3594 CCTCATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1676 GAATTC G^AATTC
EcoT22I 1148 ATGCAT ATGCA^T
FspI 2581 TGCGCA TGC^GCA
KpnI 3191 GGTACC GGTAC^C
KspI 363 CCGCGG CCGC^GG
MamI 130 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3324 CAATTG C^AATTG
MroI 2558 TCCGGA T^CCGGA
MunI 3324 CAATTG C^AATTG
NdeI 1385 CATATG CA^TATG
NsiI 1148 ATGCAT ATGCA^T
PflFI 996 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 829 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1356 ACCGGT A^CCGGT
PmaCI 2770 CACGTG CAC^GTG
PmlI 2770 CACGTG CAC^GTG
PpuMI 966 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 1280 CAGCTG CAG^CTG
RcaI 2810 TCATGA T^CATGA
SacII 363 CCGCGG CCGC^GG
SphI 778 GCATGC GCATG^C
SspI 4005 AATATT AAT^ATT
StuI 2381 AGGCCT AGG^CCT
Tth111I 996 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 829 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3650 TCTAGA T^CTAGA
XcmI 1487 CCACAGACGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 2071 GACGCC G[GA]^CG[CT]C
2613 GACGCC
AlwNI 1236 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
1265 CAGCGTCTG
AocI 1196 CCTTAGG CC^T[ACGT]AGG
3578 CCTCAGG
Aor51HI 1931 AGCGCT AGC^GCT
2778 AGCGCT
BanI 3191 GGTACC G^G[CT][GA]CC
3613 GGCACC
BanII 2467 GAGCTC G[GA]GC[CT]^C
2765 GAGCTC
BsaHI 2071 GACGCC G[GA]^CG[CT]C
2613 GACGCC
Bsp1407I 275 TGTACA T^GTACA
299 TGTACA
BspDI 1477 ATCGAT AT^CGAT
2026 ATCGAT
BsrDI 1845 CATTGC GCAATG(2/0)
2739 CATTGC
BsrGI 275 TGTACA T^GTACA
299 TGTACA
BstXI 1700 CCATGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1767 CCAGATTGCTGG
Bsu36I 1196 CCTTAGG CC^T[ACGT]AGG
3578 CCTCAGG
ClaI 1477 ATCGAT AT^CGAT
2026 ATCGAT
DraII 966 GGGACCC [GA]G^G[ACGT]CC[CT]
3175 AGGCCCC
Eco47III 1931 AGCGCT AGC^GCT
2778 AGCGCT
Eco81I 1196 CCTTAGG CC^T[ACGT]AGG
3578 CCTCAGG
EcoO109I 966 GGGACCC [GA]G^G[ACGT]CC[CT]
3175 AGGCCCC
HaeII 1931 AGCGCT [GA]GCGC^[CT]
2778 AGCGCT
Hin1I 2071 GACGCC G[GA]^CG[CT]C
2613 GACGCC
HincII 1230 GTCAAC GT[CT]^[GA]AC
3717 GTTGAC
HindII 1230 GTCAAC GT[CT]^[GA]AC
3717 GTTGAC
HindIII 1102 AAGCTT A^AGCTT
3265 AAGCTT
PaeR7I 643 CTCGAG C^TCGAG
1142 CTCGAG
SacI 2467 GAGCTC GAGCT^C
2765 GAGCTC
SspBI 275 TGTACA T^GTACA
299 TGTACA
XhoI 643 CTCGAG C^TCGAG
1142 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI Alw44I AlwI ApaLI ApoI
AspHI AvaI AvaII BamHI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII