################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII AclI AflII ApaI AscI AseI AsnI Asp700I BalI BbeI BbvCI BclI BfrI BglI BsgI BsiWI BsmBI BspMI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CpoI EagI EclXI Eco52I EcoO65I EcoRV FbaI FseI HpaI KasI MluI MluNI MscI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 3191 GGTACC G^GTACC AccIII 2558 TCCGGA T^CCGGA AflIII 2669 ACATGT A^C[GA][CT]GT AgeI 1356 ACCGGT A^CCGGT AhdI 3584 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 3191 GGTACC G^GTACC AspEI 3584 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 996 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2581 TGCGCA TGC^GCA AvrII 3722 CCTAGG C^CTAGG BbrPI 2770 CACGTG CAC^GTG BcgI 1839 CGACACCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 359 GTATCC GTATCC(6/5) BglII 1986 AGATCT A^GATCT BlnI 3722 CCTAGG C^CTAGG BlpI 1712 GCTTAGC GC^T[ACGT]AGC Bpu1102I 1712 GCTTAGC GC^T[ACGT]AGC BsaAI 2770 CACGTG [CT]AC^GT[GA] BsaBI 130 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1696 GAGACC GGTCTC(1/5) BseAI 2558 TCCGGA T^CCGGA BsiEI 42 CGGTCG CG[GA][CT]^CG BspEI 2558 TCCGGA T^CCGGA BspHI 2810 TCATGA T^CATGA BspLU11I 2669 ACATGT A^CATGT BstAPI 253 GCAAACGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 1712 GCTTAGC GC^T[ACGT]AGC DraIII 1754 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2908 GACTTAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 3584 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 3594 CCTCATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1676 GAATTC G^AATTC EcoT22I 1148 ATGCAT ATGCA^T FspI 2581 TGCGCA TGC^GCA KpnI 3191 GGTACC GGTAC^C KspI 363 CCGCGG CCGC^GG MamI 130 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3324 CAATTG C^AATTG MroI 2558 TCCGGA T^CCGGA MunI 3324 CAATTG C^AATTG NdeI 1385 CATATG CA^TATG NsiI 1148 ATGCAT ATGCA^T PflFI 996 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 829 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1356 ACCGGT A^CCGGT PmaCI 2770 CACGTG CAC^GTG PmlI 2770 CACGTG CAC^GTG PpuMI 966 GGGACCC [GA]G^G[AT]CC[CT] PvuII 1280 CAGCTG CAG^CTG RcaI 2810 TCATGA T^CATGA SacII 363 CCGCGG CCGC^GG SphI 778 GCATGC GCATG^C SspI 4005 AATATT AAT^ATT StuI 2381 AGGCCT AGG^CCT Tth111I 996 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 829 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3650 TCTAGA T^CTAGA XcmI 1487 CCACAGACGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 2071 GACGCC G[GA]^CG[CT]C 2613 GACGCC AlwNI 1236 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG 1265 CAGCGTCTG AocI 1196 CCTTAGG CC^T[ACGT]AGG 3578 CCTCAGG Aor51HI 1931 AGCGCT AGC^GCT 2778 AGCGCT BanI 3191 GGTACC G^G[CT][GA]CC 3613 GGCACC BanII 2467 GAGCTC G[GA]GC[CT]^C 2765 GAGCTC BsaHI 2071 GACGCC G[GA]^CG[CT]C 2613 GACGCC Bsp1407I 275 TGTACA T^GTACA 299 TGTACA BspDI 1477 ATCGAT AT^CGAT 2026 ATCGAT BsrDI 1845 CATTGC GCAATG(2/0) 2739 CATTGC BsrGI 275 TGTACA T^GTACA 299 TGTACA BstXI 1700 CCATGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1767 CCAGATTGCTGG Bsu36I 1196 CCTTAGG CC^T[ACGT]AGG 3578 CCTCAGG ClaI 1477 ATCGAT AT^CGAT 2026 ATCGAT DraII 966 GGGACCC [GA]G^G[ACGT]CC[CT] 3175 AGGCCCC Eco47III 1931 AGCGCT AGC^GCT 2778 AGCGCT Eco81I 1196 CCTTAGG CC^T[ACGT]AGG 3578 CCTCAGG EcoO109I 966 GGGACCC [GA]G^G[ACGT]CC[CT] 3175 AGGCCCC HaeII 1931 AGCGCT [GA]GCGC^[CT] 2778 AGCGCT Hin1I 2071 GACGCC G[GA]^CG[CT]C 2613 GACGCC HincII 1230 GTCAAC GT[CT]^[GA]AC 3717 GTTGAC HindII 1230 GTCAAC GT[CT]^[GA]AC 3717 GTTGAC HindIII 1102 AAGCTT A^AGCTT 3265 AAGCTT PaeR7I 643 CTCGAG C^TCGAG 1142 CTCGAG SacI 2467 GAGCTC GAGCT^C 2765 GAGCTC SspBI 275 TGTACA T^GTACA 299 TGTACA XhoI 643 CTCGAG C^TCGAG 1142 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI Alw44I AlwI ApaLI ApoI AspHI AvaI AvaII BamHI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII