##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccIII AclI AgeI AhdI Aor51HI
ApaI AscI AspEI AspI AviII
AvrII BbeI BcgI BlnI BsaI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BsrFI BssHII Bst1107I BstEII
BstPI BstZ17I Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV FseI FspI
KasI MfeI MluI MroI MunI
NaeI NarI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmeI Psp1406I PvuI RsrII SalI
ScaI SexAI SfoI SgrAI SmaI
SnaBI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 2272 GACGTC GACGT^C
Acc65I 512 GGTACC G^GTACC
AccI 3215 GTAGAC GT^[AC][GT]AC
AflII 2766 CTTAAG C^TTAAG
Alw44I 3978 GTGCAC G^TGCAC
ApaLI 3978 GTGCAC G^TGCAC
AseI 3900 ATTAAT AT^TAAT
AsnI 3900 ATTAAT AT^TAAT
Asp718I 512 GGTACC G^GTACC
BalI 2329 TGGCCA TGG^CCA
BamHI 932 GGATCC G^GATCC
BciVI 3263 GTATCC GTATCC(6/5)
BclI 1420 TGATCA T^GATCA
BfrI 2766 CTTAAG C^TTAAG
BglI 1687 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 927 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 3012 GAGACG CGTCTC(1/5)
BspHI 3392 TCATGA T^CATGA
BspMI 2825 ACCTGC ACCTGC(4/8)
BssSI 2604 CACGAG CACGAG(-5/-1)
BstBI 610 TTCGAA TT^CGAA
DrdI 2801 GACCTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3133 CCTGTGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1420 TGATCA T^GATCA
HaeII 1136 AGCGCC [GA]GCGC^[CT]
HincII 3269 GTTAAC GT[CT]^[GA]AC
HindII 3269 GTTAAC GT[CT]^[GA]AC
HindIII 3074 AAGCTT A^AGCTT
HpaI 3269 GTTAAC GTT^AAC
KpnI 512 GGTACC GGTAC^C
KspI 2862 CCGCGG CCGC^GG
MamI 927 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2329 TGGCCA TGG^CCA
MscI 2329 TGGCCA TGG^CCA
NcoI 1433 CCATGG C^CATGG
NspV 610 TTCGAA TT^CGAA
PflMI 1332 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3985 GACACGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3900 ATTAAT AT^TAAT
RcaI 3392 TCATGA T^CATGA
SacI 3504 GAGCTC GAGCT^C
SacII 2862 CCGCGG CCGC^GG
SbfI 2826 CCTGCAGG CCTGCA^GG
SfiI 1686 GGCCAGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 610 TTCGAA TT^CGAA
SphI 2044 GCATGC GCATG^C
Sse8387I 2826 CCTGCAGG CCTGCA^GG
SspI 3405 AATATT AAT^ATT
Van91I 1332 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 2272 GACGTC G[GA]^CG[CT]C
3014 GACGCC
BbrPI 1609 CACGTG CAC^GTG
3987 CACGTG
BbsI 1803 GAAGAC GAAGAC(2/6)
2794 GAAGAC
BglII 1541 AGATCT A^GATCT
1970 AGATCT
BlpI 2253 GCTAAGC GC^T[ACGT]AGC
2727 GCTCAGC
Bpu1102I 2253 GCTAAGC GC^T[ACGT]AGC
2727 GCTCAGC
BpuAI 1803 GAAGAC GAAGAC(2/6)
2794 GAAGAC
BsaHI 2272 GACGTC G[GA]^CG[CT]C
3014 GACGCC
BspLU11I 2227 ACATGT A^CATGT
3639 ACATGT
BsrBI 15 CCGCTC CCGCTC(-3/-3)
2985 GAGCGG
BstXI 1849 CCAAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3361 CCAGATGGATGG
CelII 2253 GCTAAGC GC^T[ACGT]AGC
2727 GCTCAGC
DraIII 427 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
3726 CACCCGGTG
EaeI 2329 TGGCCA [CT]^GGCC[GA]
2696 TGGCCG
EcoT22I 3457 ATGCAT ATGCA^T
3768 ATGCAT
Hin1I 2272 GACGTC G[GA]^CG[CT]C
3014 GACGCC
NdeI 3248 CATATG CA^TATG
3630 CATATG
NsiI 3457 ATGCAT ATGCA^T
3768 ATGCAT
PmaCI 1609 CACGTG CAC^GTG
3987 CACGTG
PmlI 1609 CACGTG CAC^GTG
3987 CACGTG
SapI 201 GAAGAGC GCTCTTC(1/4)
1980 GAAGAGC
SpeI 1650 ACTAGT A^CTAGT
3238 ACTAGT
StuI 644 AGGCCT AGG^CCT
3160 AGGCCT
TaqI 611 TCGA T^CGA
1034 TCGA
TthHB8I 611 TCGA T^CGA
1034 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BfaI
BmyI BpmI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII
XmnI