################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AccIII AclI AgeI AhdI Aor51HI ApaI AscI AspEI AspI AviII AvrII BbeI BcgI BlnI BsaI BsaWI BseAI BsiEI BsiWI BspDI BspEI BsrFI BssHII Bst1107I BstEII BstPI BstZ17I Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FseI FspI KasI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmeI Psp1406I PvuI RsrII SalI ScaI SexAI SfoI SgrAI SmaI SnaBI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 2272 GACGTC GACGT^C Acc65I 512 GGTACC G^GTACC AccI 3215 GTAGAC GT^[AC][GT]AC AflII 2766 CTTAAG C^TTAAG Alw44I 3978 GTGCAC G^TGCAC ApaLI 3978 GTGCAC G^TGCAC AseI 3900 ATTAAT AT^TAAT AsnI 3900 ATTAAT AT^TAAT Asp718I 512 GGTACC G^GTACC BalI 2329 TGGCCA TGG^CCA BamHI 932 GGATCC G^GATCC BciVI 3263 GTATCC GTATCC(6/5) BclI 1420 TGATCA T^GATCA BfrI 2766 CTTAAG C^TTAAG BglI 1687 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 927 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 3012 GAGACG CGTCTC(1/5) BspHI 3392 TCATGA T^CATGA BspMI 2825 ACCTGC ACCTGC(4/8) BssSI 2604 CACGAG CACGAG(-5/-1) BstBI 610 TTCGAA TT^CGAA DrdI 2801 GACCTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 3133 CCTGTGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 1420 TGATCA T^GATCA HaeII 1136 AGCGCC [GA]GCGC^[CT] HincII 3269 GTTAAC GT[CT]^[GA]AC HindII 3269 GTTAAC GT[CT]^[GA]AC HindIII 3074 AAGCTT A^AGCTT HpaI 3269 GTTAAC GTT^AAC KpnI 512 GGTACC GGTAC^C KspI 2862 CCGCGG CCGC^GG MamI 927 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2329 TGGCCA TGG^CCA MscI 2329 TGGCCA TGG^CCA NcoI 1433 CCATGG C^CATGG NspV 610 TTCGAA TT^CGAA PflMI 1332 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 3985 GACACGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3900 ATTAAT AT^TAAT RcaI 3392 TCATGA T^CATGA SacI 3504 GAGCTC GAGCT^C SacII 2862 CCGCGG CCGC^GG SbfI 2826 CCTGCAGG CCTGCA^GG SfiI 1686 GGCCAGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 610 TTCGAA TT^CGAA SphI 2044 GCATGC GCATG^C Sse8387I 2826 CCTGCAGG CCTGCA^GG SspI 3405 AATATT AAT^ATT Van91I 1332 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 2272 GACGTC G[GA]^CG[CT]C 3014 GACGCC BbrPI 1609 CACGTG CAC^GTG 3987 CACGTG BbsI 1803 GAAGAC GAAGAC(2/6) 2794 GAAGAC BglII 1541 AGATCT A^GATCT 1970 AGATCT BlpI 2253 GCTAAGC GC^T[ACGT]AGC 2727 GCTCAGC Bpu1102I 2253 GCTAAGC GC^T[ACGT]AGC 2727 GCTCAGC BpuAI 1803 GAAGAC GAAGAC(2/6) 2794 GAAGAC BsaHI 2272 GACGTC G[GA]^CG[CT]C 3014 GACGCC BspLU11I 2227 ACATGT A^CATGT 3639 ACATGT BsrBI 15 CCGCTC CCGCTC(-3/-3) 2985 GAGCGG BstXI 1849 CCAAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3361 CCAGATGGATGG CelII 2253 GCTAAGC GC^T[ACGT]AGC 2727 GCTCAGC DraIII 427 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 3726 CACCCGGTG EaeI 2329 TGGCCA [CT]^GGCC[GA] 2696 TGGCCG EcoT22I 3457 ATGCAT ATGCA^T 3768 ATGCAT Hin1I 2272 GACGTC G[GA]^CG[CT]C 3014 GACGCC NdeI 3248 CATATG CA^TATG 3630 CATATG NsiI 3457 ATGCAT ATGCA^T 3768 ATGCAT PmaCI 1609 CACGTG CAC^GTG 3987 CACGTG PmlI 1609 CACGTG CAC^GTG 3987 CACGTG SapI 201 GAAGAGC GCTCTTC(1/4) 1980 GAAGAGC SpeI 1650 ACTAGT A^CTAGT 3238 ACTAGT StuI 644 AGGCCT AGG^CCT 3160 AGGCCT TaqI 611 TCGA T^CGA 1034 TCGA TthHB8I 611 TCGA T^CGA 1034 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI