################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII AflII AhdI AscI Asp700I AspEI AvrII BalI BbvCI BcgI BfrI BlnI BlpI Bpu1102I BsaBI BsiWI BspHI BssHII BssSI BstEII BstPI BstXI CelII CpoI Eam1105I EcoO65I EcoT22I FseI HpaI MamI MfeI MluI MluNI MscI MunI NaeI NcoI NgoMIV NotI NruI NsiI PmeI PvuI RcaI RsrII SalI SbfI SexAI SfiI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 4154 GGTACC G^GTACC AccIII 1648 TCCGGA T^CCGGA AclI 2092 AACGTT AA^CGTT AgeI 1003 ACCGGT A^CCGGT Alw44I 4097 GTGCAC G^TGCAC ApaLI 4097 GTGCAC G^TGCAC Asp718I 4154 GGTACC G^GTACC AviII 2927 TGCGCA TGC^GCA BamHI 2123 GGATCC G^GATCC BbeI 2446 GGCGCC GGCGC^C BglI 230 GCCGGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 3893 GAGACC GGTCTC(1/5) BseAI 1648 TCCGGA T^CCGGA BspDI 1630 ATCGAT AT^CGAT BspEI 1648 TCCGGA T^CCGGA BsrBI 101 CCGCTC CCGCTC(-3/-3) BsrDI 2299 CATTGC GCAATG(2/0) Bst1107I 4592 GTATAC GTA^TAC BstAPI 3133 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1881 TTCGAA TT^CGAA BstZ17I 4592 GTATAC GTA^TAC ClaI 1630 ATCGAT AT^CGAT DrdI 3006 GACTGGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 795 GAATTC G^AATTC EcoRV 1028 GATATC GAT^ATC FspI 2927 TGCGCA TGC^GCA HincII 1394 GTCAAC GT[CT]^[GA]AC HindII 1394 GTCAAC GT[CT]^[GA]AC KasI 2446 GGCGCC G^GCGCC KpnI 4154 GGTACC GGTAC^C KspI 221 CCGCGG CCGC^GG MroI 1648 TCCGGA T^CCGGA MslI 5063 CATTTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 2446 GGCGCC GG^CGCC NheI 822 GCTAGC G^CTAGC NspV 1881 TTCGAA TT^CGAA PacI 1635 TTAATTAA TTAAT^TAA PaeR7I 34 CTCGAG C^TCGAG PinAI 1003 ACCGGT A^CCGGT Psp1406I 2092 AACGTT AA^CGTT SacI 3884 GAGCTC GAGCT^C SacII 221 CCGCGG CCGC^GG SfoI 2446 GGCGCC GGC^GCC SfuI 1881 TTCGAA TT^CGAA SphI 2528 GCATGC GCATG^C XcmI 2945 CCAGCAGATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 34 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 1037 GTCTAC GT^[AC][GT]AC 4592 GTATAC AcyI 2446 GGCGCC G[GA]^CG[CT]C 4615 GACGCC AflIII 829 ACATGT A^C[GA][CT]GT 5025 ACATGT Aor51HI 635 AGCGCT AGC^GCT 1306 AGCGCT ApaI 54 GGGCCC GGGCC^C 2626 GGGCCC AseI 1634 ATTAAT AT^TAAT 1638 ATTAAT AsnI 1634 ATTAAT AT^TAAT 1638 ATTAAT AvaI 34 CTCGAG C^[CT]CG[GA]G 769 CTCGGG BciVI 2635 GGATAC GTATCC(6/5) 1517 GTATCC BglII 945 AGATCT A^GATCT 4020 AGATCT BsaHI 2446 GGCGCC G[GA]^CG[CT]C 4615 GACGCC BsaWI 1003 ACCGGT [AT]^CCGG[AT] 1648 TCCGGA BsmBI 153 CGTCTC CGTCTC(1/5) 1492 CGTCTC BsmI 533 GAATGC GAATGC(1/-1) 629 GAATGC BsoBI 34 CTCGAG C^[CT]CG[GA]G 769 CTCGGG BspLU11I 829 ACATGT A^CATGT 5025 ACATGT BsrFI 230 GCCGGT [GA]^CCGG[CT] 1003 ACCGGT Cfr10I 230 GCCGGT [GA]^CCGG[CT] 1003 ACCGGT DraIII 3253 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG 3318 CACGATGTG Eco47III 635 AGCGCT AGC^GCT 1306 AGCGCT Hin1I 2446 GGCGCC G[GA]^CG[CT]C 4615 GACGCC HindIII 4966 AAGCTT A^AGCTT 5019 AAGCTT PshBI 1634 ATTAAT AT^TAAT 1638 ATTAAT SapI 2548 GAAGAGC GCTCTTC(1/4) 103 GCTCTTC SmlI 34 CTCGAG C^T[CT][GA]AG 2400 CTCAAG SspI 1610 AATATT AAT^ATT 3813 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AspI AvaII BanI BanII BbrPI BbsI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PmaCI PmlI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XhoII