##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AflII AhdI AscI Asp700I
AspEI AvrII BalI BbvCI BcgI
BfrI BlnI BlpI Bpu1102I BsaBI
BsiWI BspHI BssHII BssSI BstEII
BstPI BstXI CelII CpoI Eam1105I
EcoO65I EcoT22I FseI HpaI MamI
MfeI MluI MluNI MscI MunI
NaeI NcoI NgoMIV NotI NruI
NsiI PmeI PvuI RcaI RsrII
SalI SbfI SexAI SfiI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
SwaI XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 4154 GGTACC G^GTACC
AccIII 1648 TCCGGA T^CCGGA
AclI 2092 AACGTT AA^CGTT
AgeI 1003 ACCGGT A^CCGGT
Alw44I 4097 GTGCAC G^TGCAC
ApaLI 4097 GTGCAC G^TGCAC
Asp718I 4154 GGTACC G^GTACC
AviII 2927 TGCGCA TGC^GCA
BamHI 2123 GGATCC G^GATCC
BbeI 2446 GGCGCC GGCGC^C
BglI 230 GCCGGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 3893 GAGACC GGTCTC(1/5)
BseAI 1648 TCCGGA T^CCGGA
BspDI 1630 ATCGAT AT^CGAT
BspEI 1648 TCCGGA T^CCGGA
BsrBI 101 CCGCTC CCGCTC(-3/-3)
BsrDI 2299 CATTGC GCAATG(2/0)
Bst1107I 4592 GTATAC GTA^TAC
BstAPI 3133 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1881 TTCGAA TT^CGAA
BstZ17I 4592 GTATAC GTA^TAC
ClaI 1630 ATCGAT AT^CGAT
DrdI 3006 GACTGGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 795 GAATTC G^AATTC
EcoRV 1028 GATATC GAT^ATC
FspI 2927 TGCGCA TGC^GCA
HincII 1394 GTCAAC GT[CT]^[GA]AC
HindII 1394 GTCAAC GT[CT]^[GA]AC
KasI 2446 GGCGCC G^GCGCC
KpnI 4154 GGTACC GGTAC^C
KspI 221 CCGCGG CCGC^GG
MroI 1648 TCCGGA T^CCGGA
MslI 5063 CATTTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 2446 GGCGCC GG^CGCC
NheI 822 GCTAGC G^CTAGC
NspV 1881 TTCGAA TT^CGAA
PacI 1635 TTAATTAA TTAAT^TAA
PaeR7I 34 CTCGAG C^TCGAG
PinAI 1003 ACCGGT A^CCGGT
Psp1406I 2092 AACGTT AA^CGTT
SacI 3884 GAGCTC GAGCT^C
SacII 221 CCGCGG CCGC^GG
SfoI 2446 GGCGCC GGC^GCC
SfuI 1881 TTCGAA TT^CGAA
SphI 2528 GCATGC GCATG^C
XcmI 2945 CCAGCAGATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 34 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1037 GTCTAC GT^[AC][GT]AC
4592 GTATAC
AcyI 2446 GGCGCC G[GA]^CG[CT]C
4615 GACGCC
AflIII 829 ACATGT A^C[GA][CT]GT
5025 ACATGT
Aor51HI 635 AGCGCT AGC^GCT
1306 AGCGCT
ApaI 54 GGGCCC GGGCC^C
2626 GGGCCC
AseI 1634 ATTAAT AT^TAAT
1638 ATTAAT
AsnI 1634 ATTAAT AT^TAAT
1638 ATTAAT
AvaI 34 CTCGAG C^[CT]CG[GA]G
769 CTCGGG
BciVI 2635 GGATAC GTATCC(6/5)
1517 GTATCC
BglII 945 AGATCT A^GATCT
4020 AGATCT
BsaHI 2446 GGCGCC G[GA]^CG[CT]C
4615 GACGCC
BsaWI 1003 ACCGGT [AT]^CCGG[AT]
1648 TCCGGA
BsmBI 153 CGTCTC CGTCTC(1/5)
1492 CGTCTC
BsmI 533 GAATGC GAATGC(1/-1)
629 GAATGC
BsoBI 34 CTCGAG C^[CT]CG[GA]G
769 CTCGGG
BspLU11I 829 ACATGT A^CATGT
5025 ACATGT
BsrFI 230 GCCGGT [GA]^CCGG[CT]
1003 ACCGGT
Cfr10I 230 GCCGGT [GA]^CCGG[CT]
1003 ACCGGT
DraIII 3253 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
3318 CACGATGTG
Eco47III 635 AGCGCT AGC^GCT
1306 AGCGCT
Hin1I 2446 GGCGCC G[GA]^CG[CT]C
4615 GACGCC
HindIII 4966 AAGCTT A^AGCTT
5019 AAGCTT
PshBI 1634 ATTAAT AT^TAAT
1638 ATTAAT
SapI 2548 GAAGAGC GCTCTTC(1/4)
103 GCTCTTC
SmlI 34 CTCGAG C^T[CT][GA]AG
2400 CTCAAG
SspI 1610 AATATT AAT^ATT
3813 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AspI AvaII BanI BanII BbrPI
BbsI BbvI BclI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PmaCI
PmlI PpuMI PshAI PstI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XhoII