##################################
# NO CUT ENZYMES (   64 enzymes) #
##################################

AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AspEI       AviII       
AvrII       BciVI       BfrI        BglI        BlnI        
BsiWI       BspDI       BspHI       BssHII      BssSI       
Bst1107I    BstAPI      BstZ17I     ClaI        CpoI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoRV       EcoT22I     FauI        FseI        
FspI        HpaI        KspI        MfeI        MunI        
NdeI        NotI        NruI        NsiI        PacI        
PinAI       PmeI        RcaI        RsrII       SacI        
SacII       SbfI        SfiI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    StuI        
XbaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AatII        1880  GACGTC               GACGT^C
AccII        3735  CGCG                 CG^CG
AccIII       1556  TCCGGA               T^CCGGA
AclI         1321  AACGTT               AA^CGTT
Asp700I      1976  GAACCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          583  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         3064  TGGCCA               TGG^CCA
BanII         758  GAGCCC               G[GA]GC[CT]^C
BbrPI        1237  CACGTG               CAC^GTG
BbvCI         250  GCTGAGG              CCTCAGC(-5/-2)
BcgI         3545  CGACTGCTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          835  CCCGG                CC^[GC]GG
BlpI         2955  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     2955  GCTTAGC              GC^T[ACGT]AGC
BseAI        1556  TCCGGA               T^CCGGA
BsiEI        1150  CGATCG               CG[GA][CT]^CG
BsmBI        2154  CGTCTC               CGTCTC(1/5)
BspEI        1556  TCCGGA               T^CCGGA
BspLU11I     3149  ACATGT               A^CATGT
BsrBI        2794  GAGCGG               CCGCTC(-3/-3)
BsrFI         908  GCCGGC               [GA]^CCGG[CT]
BstEII       1822  GGTGACC              G^GT[ACGT]ACC
BstPI        1822  GGTGACC              G^GT[ACGT]ACC
BstUI        3735  CGCG                 CG^CG
CelII        2955  GCTTAGC              GC^T[ACGT]AGC
Cfr10I        908  GCCGGC               [GA]^CCGG[CT]
DraIII       1234  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1146  GACCCGATCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          736  CCATGG               C^C[GA][CT]GG
EcoO65I      1822  GGTGACC              G^GT[ACGT]ACC
EcoRI         564  GAATTC               G^AATTC
HindIII      3889  AAGCTT               A^AGCTT
MluI         3734  ACGCGT               A^CGCGT
MluNI        3064  TGGCCA               TGG^CCA
MroI         1556  TCCGGA               T^CCGGA
MscI         3064  TGGCCA               TGG^CCA
MvnI         3735  CGCG                 CG^CG
NaeI          908  GCCGGC               GCC^GGC
NciI          835  CCCGG                CC^[GC]GG
NcoI          736  CCATGG               C^CATGG
NgoMIV        908  GCCGGC               G^CCGGC
NheI         2866  GCTAGC               G^CTAGC
PaeR7I        224  CTCGAG               C^TCGAG
PflFI         583  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1237  CACGTG               CAC^GTG
PmlI         1237  CACGTG               CAC^GTG
PshAI        2480  GACGACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     1321  AACGTT               AA^CGTT
PvuI         1150  CGATCG               CGAT^CG
PvuII         569  CAGCTG               CAG^CTG
SalI         3543  GTCGAC               G^TCGAC
SapI         1216  GAAGAGC              GCTCTTC(1/4)
SexAI         584  ACCTGGT              A^CC[AT]GGT
SmlI          224  CTCGAG               C^T[CT][GA]AG
SwaI         3625  ATTTAAAT             ATTT^AAAT
Tth111I       583  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          224  CTCGAG               C^TCGAG
XmnI         1976  GAACCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   42 enzymes) #
###################################

Acc65I        732  GGTACC               G^GTACC
             2928  GGTACC              
AccI          527  GTAGAC               GT^[AC][GT]AC
             3543  GTCGAC              
AlwNI        2219  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2450  CAGCTCCTG           
AocI         1286  CCTCAGG              CC^T[ACGT]AGG
             1970  CCTGAGG             
AseI         1490  ATTAAT               AT^TAAT
             3407  ATTAAT              
AsnI         1490  ATTAAT               AT^TAAT
             3407  ATTAAT              
Asp718I       732  GGTACC               G^GTACC
             2928  GGTACC              
AspHI          17  GTGCTC               G[AT]GC[AT]^C
             2063  GAGCAC              
AvaI          224  CTCGAG               C^[CT]CG[GA]G
             1818  CTCGGG              
BamHI        1609  GGATCC               G^GATCC
             1966  GGATCC              
BbeI         2193  GGCGCC               GGCGC^C
             2803  GGCGCC              
BbsI         1936  GAAGAC               GAAGAC(2/6)
               78  GTCTTC              
BpuAI        1936  GAAGAC               GAAGAC(2/6)
               78  GTCTTC              
BsaBI         709  GATCAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1274  GATGAGAATC          
BsaI         1506  GGTCTC               GGTCTC(1/5)
             2411  GGTCTC              
BsaWI        1556  TCCGGA               [AT]^CCGG[AT]
             2100  TCCGGT              
BsiHKAI        17  GTGCTC               G[AT]GC[AT]^C
             2063  GAGCAC              
BsoBI         224  CTCGAG               C^[CT]CG[GA]G
             1818  CTCGGG              
Bsp1407I     2232  TGTACA               T^GTACA
             2773  TGTACA              
BspMI        1843  GCAGGT               ACCTGC(4/8)
             2352  GCAGGT              
BsrGI        2232  TGTACA               T^GTACA
             2773  TGTACA              
BstBI         450  TTCGAA               TT^CGAA
             2357  TTCGAA              
BstXI        1119  CCAACACAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3055  CCAGGTTAATGG        
Bsu36I       1286  CCTCAGG              CC^T[ACGT]AGG
             1970  CCTGAGG             
DraII        2055  AGGACCC              [GA]G^G[ACGT]CC[CT]
             2397  GGGACCT             
EarI         1216  GAAGAG               CTCTTC(1/4)
             1521  GAAGAG              
Eco81I       1286  CCTCAGG              CC^T[ACGT]AGG
             1970  CCTGAGG             
EcoO109I     2055  AGGACCC              [GA]G^G[ACGT]CC[CT]
             2397  GGGACCT             
KasI         2193  GGCGCC               G^GCGCC
             2803  GGCGCC              
KpnI          732  GGTACC               GGTAC^C
             2928  GGTACC              
Ksp632I      1216  GAAGAG               CTCTTC(1/4)
             1521  GAAGAG              
MamI          709  GATCAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1274  GATGAGAATC          
NarI         2193  GGCGCC               GG^CGCC
             2803  GGCGCC              
NspV          450  TTCGAA               TT^CGAA
             2357  TTCGAA              
PflMI        2218  CCAGAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3741  CCATTTACTGG         
PpuMI        2055  AGGACCC              [GA]G^G[AT]CC[CT]
             2397  GGGACCT             
PshBI        1490  ATTAAT               AT^TAAT
             3407  ATTAAT              
ScaI         2443  AGTACT               AGT^ACT
             2520  AGTACT              
SfoI         2193  GGCGCC               GGC^GCC
             2803  GGCGCC              
SfuI          450  TTCGAA               TT^CGAA
             2357  TTCGAA              
SspBI        2232  TGTACA               T^GTACA
             2773  TGTACA              
Van91I       2218  CCAGAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3741  CCATTTACTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AflIII      
AluI        AlwI        ApoI        AvaII       BanI        
BbvI        BclI        BfaI        BglII       BmyI        
BpmI        BsaAI       BsaHI       BsaJI       BseRI       
BsgI        BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        EaeI        
EcoRII      EcoT14I     FbaI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII