################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AspEI AviII AvrII BciVI BfrI BglI BlnI BsiWI BspDI BspHI BssHII BssSI Bst1107I BstAPI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRV EcoT22I FauI FseI FspI HpaI KspI MfeI MunI NdeI NotI NruI NsiI PacI PinAI PmeI RcaI RsrII SacI SacII SbfI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AatII 1880 GACGTC GACGT^C AccII 3735 CGCG CG^CG AccIII 1556 TCCGGA T^CCGGA AclI 1321 AACGTT AA^CGTT Asp700I 1976 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 583 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 3064 TGGCCA TGG^CCA BanII 758 GAGCCC G[GA]GC[CT]^C BbrPI 1237 CACGTG CAC^GTG BbvCI 250 GCTGAGG CCTCAGC(-5/-2) BcgI 3545 CGACTGCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 835 CCCGG CC^[GC]GG BlpI 2955 GCTTAGC GC^T[ACGT]AGC Bpu1102I 2955 GCTTAGC GC^T[ACGT]AGC BseAI 1556 TCCGGA T^CCGGA BsiEI 1150 CGATCG CG[GA][CT]^CG BsmBI 2154 CGTCTC CGTCTC(1/5) BspEI 1556 TCCGGA T^CCGGA BspLU11I 3149 ACATGT A^CATGT BsrBI 2794 GAGCGG CCGCTC(-3/-3) BsrFI 908 GCCGGC [GA]^CCGG[CT] BstEII 1822 GGTGACC G^GT[ACGT]ACC BstPI 1822 GGTGACC G^GT[ACGT]ACC BstUI 3735 CGCG CG^CG CelII 2955 GCTTAGC GC^T[ACGT]AGC Cfr10I 908 GCCGGC [GA]^CCGG[CT] DraIII 1234 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1146 GACCCGATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 736 CCATGG C^C[GA][CT]GG EcoO65I 1822 GGTGACC G^GT[ACGT]ACC EcoRI 564 GAATTC G^AATTC HindIII 3889 AAGCTT A^AGCTT MluI 3734 ACGCGT A^CGCGT MluNI 3064 TGGCCA TGG^CCA MroI 1556 TCCGGA T^CCGGA MscI 3064 TGGCCA TGG^CCA MvnI 3735 CGCG CG^CG NaeI 908 GCCGGC GCC^GGC NciI 835 CCCGG CC^[GC]GG NcoI 736 CCATGG C^CATGG NgoMIV 908 GCCGGC G^CCGGC NheI 2866 GCTAGC G^CTAGC PaeR7I 224 CTCGAG C^TCGAG PflFI 583 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1237 CACGTG CAC^GTG PmlI 1237 CACGTG CAC^GTG PshAI 2480 GACGACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1321 AACGTT AA^CGTT PvuI 1150 CGATCG CGAT^CG PvuII 569 CAGCTG CAG^CTG SalI 3543 GTCGAC G^TCGAC SapI 1216 GAAGAGC GCTCTTC(1/4) SexAI 584 ACCTGGT A^CC[AT]GGT SmlI 224 CTCGAG C^T[CT][GA]AG SwaI 3625 ATTTAAAT ATTT^AAAT Tth111I 583 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 224 CTCGAG C^TCGAG XmnI 1976 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 732 GGTACC G^GTACC 2928 GGTACC AccI 527 GTAGAC GT^[AC][GT]AC 3543 GTCGAC AlwNI 2219 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 2450 CAGCTCCTG AocI 1286 CCTCAGG CC^T[ACGT]AGG 1970 CCTGAGG AseI 1490 ATTAAT AT^TAAT 3407 ATTAAT AsnI 1490 ATTAAT AT^TAAT 3407 ATTAAT Asp718I 732 GGTACC G^GTACC 2928 GGTACC AspHI 17 GTGCTC G[AT]GC[AT]^C 2063 GAGCAC AvaI 224 CTCGAG C^[CT]CG[GA]G 1818 CTCGGG BamHI 1609 GGATCC G^GATCC 1966 GGATCC BbeI 2193 GGCGCC GGCGC^C 2803 GGCGCC BbsI 1936 GAAGAC GAAGAC(2/6) 78 GTCTTC BpuAI 1936 GAAGAC GAAGAC(2/6) 78 GTCTTC BsaBI 709 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1274 GATGAGAATC BsaI 1506 GGTCTC GGTCTC(1/5) 2411 GGTCTC BsaWI 1556 TCCGGA [AT]^CCGG[AT] 2100 TCCGGT BsiHKAI 17 GTGCTC G[AT]GC[AT]^C 2063 GAGCAC BsoBI 224 CTCGAG C^[CT]CG[GA]G 1818 CTCGGG Bsp1407I 2232 TGTACA T^GTACA 2773 TGTACA BspMI 1843 GCAGGT ACCTGC(4/8) 2352 GCAGGT BsrGI 2232 TGTACA T^GTACA 2773 TGTACA BstBI 450 TTCGAA TT^CGAA 2357 TTCGAA BstXI 1119 CCAACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3055 CCAGGTTAATGG Bsu36I 1286 CCTCAGG CC^T[ACGT]AGG 1970 CCTGAGG DraII 2055 AGGACCC [GA]G^G[ACGT]CC[CT] 2397 GGGACCT EarI 1216 GAAGAG CTCTTC(1/4) 1521 GAAGAG Eco81I 1286 CCTCAGG CC^T[ACGT]AGG 1970 CCTGAGG EcoO109I 2055 AGGACCC [GA]G^G[ACGT]CC[CT] 2397 GGGACCT KasI 2193 GGCGCC G^GCGCC 2803 GGCGCC KpnI 732 GGTACC GGTAC^C 2928 GGTACC Ksp632I 1216 GAAGAG CTCTTC(1/4) 1521 GAAGAG MamI 709 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1274 GATGAGAATC NarI 2193 GGCGCC GG^CGCC 2803 GGCGCC NspV 450 TTCGAA TT^CGAA 2357 TTCGAA PflMI 2218 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3741 CCATTTACTGG PpuMI 2055 AGGACCC [GA]G^G[AT]CC[CT] 2397 GGGACCT PshBI 1490 ATTAAT AT^TAAT 3407 ATTAAT ScaI 2443 AGTACT AGT^ACT 2520 AGTACT SfoI 2193 GGCGCC GGC^GCC 2803 GGCGCC SfuI 450 TTCGAA TT^CGAA 2357 TTCGAA SspBI 2232 TGTACA T^GTACA 2773 TGTACA Van91I 2218 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3741 CCATTTACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI AlwI ApoI AvaII BanI BbvI BclI BfaI BglII BmyI BpmI BsaAI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EaeI EcoRII EcoT14I FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII