##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AspEI AviII
AvrII BciVI BfrI BglI BlnI
BsiWI BspDI BspHI BssHII BssSI
Bst1107I BstAPI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRV EcoT22I FauI FseI
FspI HpaI KspI MfeI MunI
NdeI NotI NruI NsiI PacI
PinAI PmeI RcaI RsrII SacI
SacII SbfI SfiI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 1880 GACGTC GACGT^C
AccII 3735 CGCG CG^CG
AccIII 1556 TCCGGA T^CCGGA
AclI 1321 AACGTT AA^CGTT
Asp700I 1976 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 583 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3064 TGGCCA TGG^CCA
BanII 758 GAGCCC G[GA]GC[CT]^C
BbrPI 1237 CACGTG CAC^GTG
BbvCI 250 GCTGAGG CCTCAGC(-5/-2)
BcgI 3545 CGACTGCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 835 CCCGG CC^[GC]GG
BlpI 2955 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2955 GCTTAGC GC^T[ACGT]AGC
BseAI 1556 TCCGGA T^CCGGA
BsiEI 1150 CGATCG CG[GA][CT]^CG
BsmBI 2154 CGTCTC CGTCTC(1/5)
BspEI 1556 TCCGGA T^CCGGA
BspLU11I 3149 ACATGT A^CATGT
BsrBI 2794 GAGCGG CCGCTC(-3/-3)
BsrFI 908 GCCGGC [GA]^CCGG[CT]
BstEII 1822 GGTGACC G^GT[ACGT]ACC
BstPI 1822 GGTGACC G^GT[ACGT]ACC
BstUI 3735 CGCG CG^CG
CelII 2955 GCTTAGC GC^T[ACGT]AGC
Cfr10I 908 GCCGGC [GA]^CCGG[CT]
DraIII 1234 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1146 GACCCGATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 736 CCATGG C^C[GA][CT]GG
EcoO65I 1822 GGTGACC G^GT[ACGT]ACC
EcoRI 564 GAATTC G^AATTC
HindIII 3889 AAGCTT A^AGCTT
MluI 3734 ACGCGT A^CGCGT
MluNI 3064 TGGCCA TGG^CCA
MroI 1556 TCCGGA T^CCGGA
MscI 3064 TGGCCA TGG^CCA
MvnI 3735 CGCG CG^CG
NaeI 908 GCCGGC GCC^GGC
NciI 835 CCCGG CC^[GC]GG
NcoI 736 CCATGG C^CATGG
NgoMIV 908 GCCGGC G^CCGGC
NheI 2866 GCTAGC G^CTAGC
PaeR7I 224 CTCGAG C^TCGAG
PflFI 583 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1237 CACGTG CAC^GTG
PmlI 1237 CACGTG CAC^GTG
PshAI 2480 GACGACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1321 AACGTT AA^CGTT
PvuI 1150 CGATCG CGAT^CG
PvuII 569 CAGCTG CAG^CTG
SalI 3543 GTCGAC G^TCGAC
SapI 1216 GAAGAGC GCTCTTC(1/4)
SexAI 584 ACCTGGT A^CC[AT]GGT
SmlI 224 CTCGAG C^T[CT][GA]AG
SwaI 3625 ATTTAAAT ATTT^AAAT
Tth111I 583 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 224 CTCGAG C^TCGAG
XmnI 1976 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 732 GGTACC G^GTACC
2928 GGTACC
AccI 527 GTAGAC GT^[AC][GT]AC
3543 GTCGAC
AlwNI 2219 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2450 CAGCTCCTG
AocI 1286 CCTCAGG CC^T[ACGT]AGG
1970 CCTGAGG
AseI 1490 ATTAAT AT^TAAT
3407 ATTAAT
AsnI 1490 ATTAAT AT^TAAT
3407 ATTAAT
Asp718I 732 GGTACC G^GTACC
2928 GGTACC
AspHI 17 GTGCTC G[AT]GC[AT]^C
2063 GAGCAC
AvaI 224 CTCGAG C^[CT]CG[GA]G
1818 CTCGGG
BamHI 1609 GGATCC G^GATCC
1966 GGATCC
BbeI 2193 GGCGCC GGCGC^C
2803 GGCGCC
BbsI 1936 GAAGAC GAAGAC(2/6)
78 GTCTTC
BpuAI 1936 GAAGAC GAAGAC(2/6)
78 GTCTTC
BsaBI 709 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1274 GATGAGAATC
BsaI 1506 GGTCTC GGTCTC(1/5)
2411 GGTCTC
BsaWI 1556 TCCGGA [AT]^CCGG[AT]
2100 TCCGGT
BsiHKAI 17 GTGCTC G[AT]GC[AT]^C
2063 GAGCAC
BsoBI 224 CTCGAG C^[CT]CG[GA]G
1818 CTCGGG
Bsp1407I 2232 TGTACA T^GTACA
2773 TGTACA
BspMI 1843 GCAGGT ACCTGC(4/8)
2352 GCAGGT
BsrGI 2232 TGTACA T^GTACA
2773 TGTACA
BstBI 450 TTCGAA TT^CGAA
2357 TTCGAA
BstXI 1119 CCAACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3055 CCAGGTTAATGG
Bsu36I 1286 CCTCAGG CC^T[ACGT]AGG
1970 CCTGAGG
DraII 2055 AGGACCC [GA]G^G[ACGT]CC[CT]
2397 GGGACCT
EarI 1216 GAAGAG CTCTTC(1/4)
1521 GAAGAG
Eco81I 1286 CCTCAGG CC^T[ACGT]AGG
1970 CCTGAGG
EcoO109I 2055 AGGACCC [GA]G^G[ACGT]CC[CT]
2397 GGGACCT
KasI 2193 GGCGCC G^GCGCC
2803 GGCGCC
KpnI 732 GGTACC GGTAC^C
2928 GGTACC
Ksp632I 1216 GAAGAG CTCTTC(1/4)
1521 GAAGAG
MamI 709 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1274 GATGAGAATC
NarI 2193 GGCGCC GG^CGCC
2803 GGCGCC
NspV 450 TTCGAA TT^CGAA
2357 TTCGAA
PflMI 2218 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3741 CCATTTACTGG
PpuMI 2055 AGGACCC [GA]G^G[AT]CC[CT]
2397 GGGACCT
PshBI 1490 ATTAAT AT^TAAT
3407 ATTAAT
ScaI 2443 AGTACT AGT^ACT
2520 AGTACT
SfoI 2193 GGCGCC GGC^GCC
2803 GGCGCC
SfuI 450 TTCGAA TT^CGAA
2357 TTCGAA
SspBI 2232 TGTACA T^GTACA
2773 TGTACA
Van91I 2218 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3741 CCATTTACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI ApoI AvaII BanI
BbvI BclI BfaI BglII BmyI
BpmI BsaAI BsaHI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI EaeI
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII