##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccI AccIII AgeI AlwNI
AscI AseI AsnI AspI AviII
AvrII BlnI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrFI BssHII BssSI Bst1107I BstBI
BstZ17I Cfr10I ClaI CpoI EagI
EclXI Eco52I FseI FspI HgaI
KspI MluI MroI NaeI NdeI
NgoMIV NotI NruI NspV PacI
PflFI PflMI PinAI PmeI PshAI
PshBI PvuI RsrII SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI Sse8387I SwaI Tth111I
Van91I XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 2971 GGTACC G^GTACC
AclI 1662 AACGTT AA^CGTT
AcyI 4052 GGCGCC G[GA]^CG[CT]C
AflII 3011 CTTAAG C^TTAAG
Alw44I 498 GTGCAC G^TGCAC
AocI 2875 CCTTAGG CC^T[ACGT]AGG
Aor51HI 3489 AGCGCT AGC^GCT
ApaLI 498 GTGCAC G^TGCAC
Asp718I 2971 GGTACC G^GTACC
AvaI 42 CTCGAG C^[CT]CG[GA]G
BamHI 712 GGATCC G^GATCC
BbeI 4052 GGCGCC GGCGC^C
BbrPI 2925 CACGTG CAC^GTG
BcgI 44 CGAGTGGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2785 GGATAC GTATCC(6/5)
BfrI 3011 CTTAAG C^TTAAG
BlpI 1958 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1958 GCTCAGC GC^T[ACGT]AGC
BsaAI 2925 CACGTG [CT]AC^GT[GA]
BsaHI 4052 GGCGCC G[GA]^CG[CT]C
BsoBI 42 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1933 TGTACA T^GTACA
BsrGI 1933 TGTACA T^GTACA
BstEII 1797 GGTTACC G^GT[ACGT]ACC
BstPI 1797 GGTTACC G^GT[ACGT]ACC
Bsu36I 2875 CCTTAGG CC^T[ACGT]AGG
CelII 1958 GCTCAGC GC^T[ACGT]AGC
Eco47III 3489 AGCGCT AGC^GCT
Eco81I 2875 CCTTAGG CC^T[ACGT]AGG
EcoO65I 1797 GGTTACC G^GT[ACGT]ACC
EcoRV 730 GATATC GAT^ATC
EcoT14I 503 CCATGG C^C[AT][AT]GG
EcoT22I 4497 ATGCAT ATGCA^T
FauI 66 CCCGC CCCGC(4/6)
Hin1I 4052 GGCGCC G[GA]^CG[CT]C
HpaI 4628 GTTAAC GTT^AAC
KasI 4052 GGCGCC G^GCGCC
KpnI 2971 GGTACC GGTAC^C
MfeI 4669 CAATTG C^AATTG
MunI 4669 CAATTG C^AATTG
NarI 4052 GGCGCC GG^CGCC
NcoI 503 CCATGG C^CATGG
NheI 320 GCTAGC G^CTAGC
NsiI 4497 ATGCAT ATGCA^T
PaeR7I 42 CTCGAG C^TCGAG
PmaCI 2925 CACGTG CAC^GTG
PmlI 2925 CACGTG CAC^GTG
Psp1406I 1662 AACGTT AA^CGTT
SacI 165 GAGCTC GAGCT^C
SfoI 4052 GGCGCC GGC^GCC
SpeI 2032 ACTAGT A^CTAGT
SphI 4499 GCATGC GCATG^C
SspBI 1933 TGTACA T^GTACA
SspI 876 AATATT AAT^ATT
StuI 2161 AGGCCT AGG^CCT
StyI 503 CCATGG C^C[AT][AT]GG
TaqI 43 TCGA T^CGA
TthHB8I 43 TCGA T^CGA
XhoI 42 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AhdI 3839 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4329 GACAAATTGTC
Asp700I 806 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3304 GAATAAGTTC
AspEI 3839 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4329 GACAAATTGTC
BalI 2708 TGGCCA TGG^CCA
4397 TGGCCA
BbvCI 290 GCTGAGG CCTCAGC(-5/-2)
1265 GCTGAGG
BclI 3187 TGATCA T^GATCA
4289 TGATCA
BglI 2402 GCCCTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4055 GCCAGACAGGC
BsaBI 713 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1538 GATAACAATC
BspMI 421 GCAGGT ACCTGC(4/8)
3094 GCAGGT
BsrDI 1894 CATTGC GCAATG(2/0)
3476 GCAATG
BstAPI 1889 GCACCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4071 GCAGAGTTTGC
BstXI 1398 CCAGCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1822 CCAAATTGTTGG
DraIII 3901 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
4384 CACATAGTG
DrdI 2922 GACCACGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3909 GACTCCTCTGTC
DsaI 170 CCGTGG C^C[GA][CT]GG
503 CCATGG
EaeI 2708 TGGCCA [CT]^GGCC[GA]
4397 TGGCCA
Eam1105I 3839 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4329 GACAAATTGTC
EcoNI 2624 CCTAAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2961 CCTGGGACAGG
EcoRI 952 GAATTC G^AATTC
4140 GAATTC
FbaI 3187 TGATCA T^GATCA
4289 TGATCA
MamI 713 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1538 GATAACAATC
MluNI 2708 TGGCCA TGG^CCA
4397 TGGCCA
MscI 2708 TGGCCA TGG^CCA
4397 TGGCCA
PpuMI 3777 AGGTCCT [GA]G^G[AT]CC[CT]
4273 AGGACCC
SapI 550 GAAGAGC GCTCTTC(1/4)
2582 GAAGAGC
ScaI 1361 AGTACT AGT^ACT
4720 AGTACT
XmnI 806 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3304 GAATAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApaI ApoI AspHI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BspLU11I BsrBI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII