################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII AccI AccIII AgeI AlwNI AscI AseI AsnI AspI AviII AvrII BlnI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrFI BssHII BssSI Bst1107I BstBI BstZ17I Cfr10I ClaI CpoI EagI EclXI Eco52I FseI FspI HgaI KspI MluI MroI NaeI NdeI NgoMIV NotI NruI NspV PacI PflFI PflMI PinAI PmeI PshAI PshBI PvuI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI Sse8387I SwaI Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### Acc65I 2971 GGTACC G^GTACC AclI 1662 AACGTT AA^CGTT AcyI 4052 GGCGCC G[GA]^CG[CT]C AflII 3011 CTTAAG C^TTAAG Alw44I 498 GTGCAC G^TGCAC AocI 2875 CCTTAGG CC^T[ACGT]AGG Aor51HI 3489 AGCGCT AGC^GCT ApaLI 498 GTGCAC G^TGCAC Asp718I 2971 GGTACC G^GTACC AvaI 42 CTCGAG C^[CT]CG[GA]G BamHI 712 GGATCC G^GATCC BbeI 4052 GGCGCC GGCGC^C BbrPI 2925 CACGTG CAC^GTG BcgI 44 CGAGTGGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2785 GGATAC GTATCC(6/5) BfrI 3011 CTTAAG C^TTAAG BlpI 1958 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1958 GCTCAGC GC^T[ACGT]AGC BsaAI 2925 CACGTG [CT]AC^GT[GA] BsaHI 4052 GGCGCC G[GA]^CG[CT]C BsoBI 42 CTCGAG C^[CT]CG[GA]G Bsp1407I 1933 TGTACA T^GTACA BsrGI 1933 TGTACA T^GTACA BstEII 1797 GGTTACC G^GT[ACGT]ACC BstPI 1797 GGTTACC G^GT[ACGT]ACC Bsu36I 2875 CCTTAGG CC^T[ACGT]AGG CelII 1958 GCTCAGC GC^T[ACGT]AGC Eco47III 3489 AGCGCT AGC^GCT Eco81I 2875 CCTTAGG CC^T[ACGT]AGG EcoO65I 1797 GGTTACC G^GT[ACGT]ACC EcoRV 730 GATATC GAT^ATC EcoT14I 503 CCATGG C^C[AT][AT]GG EcoT22I 4497 ATGCAT ATGCA^T FauI 66 CCCGC CCCGC(4/6) Hin1I 4052 GGCGCC G[GA]^CG[CT]C HpaI 4628 GTTAAC GTT^AAC KasI 4052 GGCGCC G^GCGCC KpnI 2971 GGTACC GGTAC^C MfeI 4669 CAATTG C^AATTG MunI 4669 CAATTG C^AATTG NarI 4052 GGCGCC GG^CGCC NcoI 503 CCATGG C^CATGG NheI 320 GCTAGC G^CTAGC NsiI 4497 ATGCAT ATGCA^T PaeR7I 42 CTCGAG C^TCGAG PmaCI 2925 CACGTG CAC^GTG PmlI 2925 CACGTG CAC^GTG Psp1406I 1662 AACGTT AA^CGTT SacI 165 GAGCTC GAGCT^C SfoI 4052 GGCGCC GGC^GCC SpeI 2032 ACTAGT A^CTAGT SphI 4499 GCATGC GCATG^C SspBI 1933 TGTACA T^GTACA SspI 876 AATATT AAT^ATT StuI 2161 AGGCCT AGG^CCT StyI 503 CCATGG C^C[AT][AT]GG TaqI 43 TCGA T^CGA TthHB8I 43 TCGA T^CGA XhoI 42 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AhdI 3839 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4329 GACAAATTGTC Asp700I 806 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3304 GAATAAGTTC AspEI 3839 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4329 GACAAATTGTC BalI 2708 TGGCCA TGG^CCA 4397 TGGCCA BbvCI 290 GCTGAGG CCTCAGC(-5/-2) 1265 GCTGAGG BclI 3187 TGATCA T^GATCA 4289 TGATCA BglI 2402 GCCCTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4055 GCCAGACAGGC BsaBI 713 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1538 GATAACAATC BspMI 421 GCAGGT ACCTGC(4/8) 3094 GCAGGT BsrDI 1894 CATTGC GCAATG(2/0) 3476 GCAATG BstAPI 1889 GCACCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4071 GCAGAGTTTGC BstXI 1398 CCAGCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1822 CCAAATTGTTGG DraIII 3901 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG 4384 CACATAGTG DrdI 2922 GACCACGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3909 GACTCCTCTGTC DsaI 170 CCGTGG C^C[GA][CT]GG 503 CCATGG EaeI 2708 TGGCCA [CT]^GGCC[GA] 4397 TGGCCA Eam1105I 3839 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4329 GACAAATTGTC EcoNI 2624 CCTAAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2961 CCTGGGACAGG EcoRI 952 GAATTC G^AATTC 4140 GAATTC FbaI 3187 TGATCA T^GATCA 4289 TGATCA MamI 713 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1538 GATAACAATC MluNI 2708 TGGCCA TGG^CCA 4397 TGGCCA MscI 2708 TGGCCA TGG^CCA 4397 TGGCCA PpuMI 3777 AGGTCCT [GA]G^G[AT]CC[CT] 4273 AGGACCC SapI 550 GAAGAGC GCTCTTC(1/4) 2582 GAAGAGC ScaI 1361 AGTACT AGT^ACT 4720 AGTACT XmnI 806 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3304 GAATAAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI ApaI ApoI AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BspLU11I BsrBI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII