################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AccIII AclI AflII AgeI AscI Asp700I BcgI BclI BfrI BlpI Bpu1102I BsaBI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrGI BssHII Bst1107I BstBI BstZ17I CelII ClaI CpoI EagI EclXI Eco52I EcoRV FbaI FseI HincII HindII HpaI MamI MfeI MluI MroI MunI NotI NruI NspV PacI PaeR7I PinAI PmeI Psp1406I PvuI RcaI RsrII SalI ScaI SexAI SfiI SfuI SnaBI SpeI SspBI SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 955 GACGTC GACGT^C AccI 3076 GTCTAC GT^[AC][GT]AC AhdI 2493 GACAAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1059 GTGCAC G^TGCAC Aor51HI 3782 AGCGCT AGC^GCT ApaLI 1059 GTGCAC G^TGCAC AseI 4057 ATTAAT AT^TAAT AsnI 4057 ATTAAT AT^TAAT AspEI 2493 GACAAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 4086 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 482 TGCGCA TGC^GCA AvrII 2007 CCTAGG C^CTAGG BamHI 2549 GGATCC G^GATCC BbeI 673 GGCGCC GGCGC^C BbrPI 4393 CACGTG CAC^GTG BlnI 2007 CCTAGG C^CTAGG BspLU11I 1652 ACATGT A^CATGT BsrBI 3353 GAGCGG CCGCTC(-3/-3) BssSI 314 CACGAG CACGAG(-5/-1) BstAPI 1907 GCAATGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraI 2168 TTTAAA TTT^AAA DrdI 85 GACATGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2493 GACAAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3782 AGCGCT AGC^GCT EcoRI 4255 GAATTC G^AATTC FspI 482 TGCGCA TGC^GCA HindIII 2845 AAGCTT A^AGCTT KasI 673 GGCGCC G^GCGCC KspI 98 CCGCGG CCGC^GG NaeI 3 GCCGGC GCC^GGC NarI 673 GGCGCC GG^CGCC NgoMIV 3 GCCGGC G^CCGGC PflFI 4086 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 4393 CACGTG CAC^GTG PmlI 4393 CACGTG CAC^GTG PshAI 4414 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4057 ATTAAT AT^TAAT SacII 98 CCGCGG CCGC^GG SapI 2076 GAAGAGC GCTCTTC(1/4) SbfI 245 CCTGCAGG CCTGCA^GG SfoI 673 GGCGCC GGC^GCC SgrAI 669 CACCGGCG C[GA]^CCGG[CT]G SphI 3981 GCATGC GCATG^C Sse8387I 245 CCTGCAGG CCTGCA^GG Tth111I 4086 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 99 CGCG CG^CG 122 CGCG AflIII 1652 ACATGT A^C[GA][CT]GT 4394 ACGTGT AlwNI 944 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 980 CAGTCCCTG BbvCI 306 CCTCAGC CCTCAGC(-5/-2) 3686 GCTGAGG BciVI 2238 GGATAC GTATCC(6/5) 4267 GGATAC BglI 3172 GCCCTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3250 GCCTCAAGGGC BsaAI 4393 CACGTG [CT]AC^GT[GA] 4613 CACGTA BsaI 814 GAGACC GGTCTC(1/5) 3380 GGTCTC BsgI 2644 CTGCAC GTGCAG(16/14) 1962 GTGCAG BsmI 231 GAATGC GAATGC(1/-1) 4342 GAATGC BstUI 99 CGCG CG^CG 122 CGCG DraIII 277 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG 4514 CACTGGGTG EcoT22I 615 ATGCAT ATGCA^T 4601 ATGCAT HaeII 673 GGCGCC [GA]GCGC^[CT] 3782 AGCGCT HgaI 1182 GACGC GACGC(5/10) 622 GCGTC MvnI 99 CGCG CG^CG 122 CGCG NdeI 2318 CATATG CA^TATG 4496 CATATG NsiI 615 ATGCAT ATGCA^T 4601 ATGCAT SacI 1618 GAGCTC GAGCT^C 1677 GAGCTC SmaI 1387 CCCGGG CCC^GGG 3562 CCCGGG StuI 1704 AGGCCT AGG^CCT 3610 AGGCCT XbaI 2268 TCTAGA T^CTAGA 2660 TCTAGA XmaCI 1387 CCCGGG C^CCGGG 3562 CCCGGG XmaI 1387 CCCGGG C^CCGGG 3562 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### Acc65I AciI AcsI AcyI AfaI AluI AlwI AocI ApaI ApoI Asp718I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NheI NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII