##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccIII AclI AflII AgeI AscI
Asp700I BcgI BclI BfrI BlpI
Bpu1102I BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BsrGI BssHII Bst1107I BstBI
BstZ17I CelII ClaI CpoI EagI
EclXI Eco52I EcoRV FbaI FseI
HincII HindII HpaI MamI MfeI
MluI MroI MunI NotI NruI
NspV PacI PaeR7I PinAI PmeI
Psp1406I PvuI RcaI RsrII SalI
ScaI SexAI SfiI SfuI SnaBI
SpeI SspBI SspI SwaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 955 GACGTC GACGT^C
AccI 3076 GTCTAC GT^[AC][GT]AC
AhdI 2493 GACAAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1059 GTGCAC G^TGCAC
Aor51HI 3782 AGCGCT AGC^GCT
ApaLI 1059 GTGCAC G^TGCAC
AseI 4057 ATTAAT AT^TAAT
AsnI 4057 ATTAAT AT^TAAT
AspEI 2493 GACAAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4086 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 482 TGCGCA TGC^GCA
AvrII 2007 CCTAGG C^CTAGG
BamHI 2549 GGATCC G^GATCC
BbeI 673 GGCGCC GGCGC^C
BbrPI 4393 CACGTG CAC^GTG
BlnI 2007 CCTAGG C^CTAGG
BspLU11I 1652 ACATGT A^CATGT
BsrBI 3353 GAGCGG CCGCTC(-3/-3)
BssSI 314 CACGAG CACGAG(-5/-1)
BstAPI 1907 GCAATGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 2168 TTTAAA TTT^AAA
DrdI 85 GACATGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2493 GACAAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3782 AGCGCT AGC^GCT
EcoRI 4255 GAATTC G^AATTC
FspI 482 TGCGCA TGC^GCA
HindIII 2845 AAGCTT A^AGCTT
KasI 673 GGCGCC G^GCGCC
KspI 98 CCGCGG CCGC^GG
NaeI 3 GCCGGC GCC^GGC
NarI 673 GGCGCC GG^CGCC
NgoMIV 3 GCCGGC G^CCGGC
PflFI 4086 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4393 CACGTG CAC^GTG
PmlI 4393 CACGTG CAC^GTG
PshAI 4414 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4057 ATTAAT AT^TAAT
SacII 98 CCGCGG CCGC^GG
SapI 2076 GAAGAGC GCTCTTC(1/4)
SbfI 245 CCTGCAGG CCTGCA^GG
SfoI 673 GGCGCC GGC^GCC
SgrAI 669 CACCGGCG C[GA]^CCGG[CT]G
SphI 3981 GCATGC GCATG^C
Sse8387I 245 CCTGCAGG CCTGCA^GG
Tth111I 4086 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 99 CGCG CG^CG
122 CGCG
AflIII 1652 ACATGT A^C[GA][CT]GT
4394 ACGTGT
AlwNI 944 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
980 CAGTCCCTG
BbvCI 306 CCTCAGC CCTCAGC(-5/-2)
3686 GCTGAGG
BciVI 2238 GGATAC GTATCC(6/5)
4267 GGATAC
BglI 3172 GCCCTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3250 GCCTCAAGGGC
BsaAI 4393 CACGTG [CT]AC^GT[GA]
4613 CACGTA
BsaI 814 GAGACC GGTCTC(1/5)
3380 GGTCTC
BsgI 2644 CTGCAC GTGCAG(16/14)
1962 GTGCAG
BsmI 231 GAATGC GAATGC(1/-1)
4342 GAATGC
BstUI 99 CGCG CG^CG
122 CGCG
DraIII 277 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
4514 CACTGGGTG
EcoT22I 615 ATGCAT ATGCA^T
4601 ATGCAT
HaeII 673 GGCGCC [GA]GCGC^[CT]
3782 AGCGCT
HgaI 1182 GACGC GACGC(5/10)
622 GCGTC
MvnI 99 CGCG CG^CG
122 CGCG
NdeI 2318 CATATG CA^TATG
4496 CATATG
NsiI 615 ATGCAT ATGCA^T
4601 ATGCAT
SacI 1618 GAGCTC GAGCT^C
1677 GAGCTC
SmaI 1387 CCCGGG CCC^GGG
3562 CCCGGG
StuI 1704 AGGCCT AGG^CCT
3610 AGGCCT
XbaI 2268 TCTAGA T^CTAGA
2660 TCTAGA
XmaCI 1387 CCCGGG C^CCGGG
3562 CCCGGG
XmaI 1387 CCCGGG C^CCGGG
3562 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
Acc65I AciI AcsI AcyI AfaI
AluI AlwI AocI ApaI ApoI
Asp718I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstEII BstF5I BstNI BstPI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NheI NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII