##################################
# NO CUT ENZYMES (   74 enzymes) #
##################################

AccIII      AgeI        AhdI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp700I     AspEI       
AspI        AviII       BamHI       BbeI        BbvCI       
BglI        BlpI        Bpu1102I    BseAI       BsiEI       
BsiWI       BsmI        BspDI       BspEI       BsrBI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstZ17I     CelII       ClaI        CpoI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoT22I     FseI        FspI        HpaI        KasI        
KspI        MluI        MroI        NarI        NdeI        
NotI        NruI        NsiI        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshBI       PvuI        
RsrII       SacI        SacII       SalI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SnaBI       
Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII        4289  GACGTC               GACGT^C
Acc65I       3832  GGTACC               G^GTACC
AccI         1094  GTAGAC               GT^[AC][GT]AC
AccII        1552  CGCG                 CG^CG
AflII        4207  CTTAAG               C^TTAAG
Asp718I      3832  GGTACC               G^GTACC
AvaI         2301  CCCGGG               C^[CT]CG[GA]G
BbrPI        4580  CACGTG               CAC^GTG
BcgI         3697  CGATTTCTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         4207  CTTAAG               C^TTAAG
BglII        3336  AGATCT               A^GATCT
BsaBI        3535  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        2026  GAGACG               CGTCTC(1/5)
BsoBI        2301  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     4982  TGTACA               T^GTACA
BspLU11I     1817  ACATGT               A^CATGT
BsrFI        3978  GCCGGC               [GA]^CCGG[CT]
BsrGI        4982  TGTACA               T^GTACA
BstAPI       2431  GCAGGGACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        4841  TTCGAA               TT^CGAA
BstUI        1552  CGCG                 CG^CG
BstXI        3381  CCATGGACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I       3978  GCCGGC               [GA]^CCGG[CT]
DraI         2035  TTTAAA               TTT^AAA
DraIII        401  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         4229  GACAGAACTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         3381  CCATGG               C^C[GA][CT]GG
EcoRV        2101  GATATC               GAT^ATC
HaeII           1  GGCGCT               [GA]GCGC^[CT]
KpnI         3832  GGTACC               GGTAC^C
MamI         3535  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          985  CAATTG               C^AATTG
MunI          985  CAATTG               C^AATTG
MvnI         1552  CGCG                 CG^CG
NaeI         3978  GCCGGC               GCC^GGC
NcoI         3381  CCATGG               C^CATGG
NgoMIV       3978  GCCGGC               G^CCGGC
NheI         3766  GCTAGC               G^CTAGC
NspV         4841  TTCGAA               TT^CGAA
PmaCI        4580  CACGTG               CAC^GTG
PmlI         4580  CACGTG               CAC^GTG
SbfI         1732  CCTGCAGG             CCTGCA^GG
SfuI         4841  TTCGAA               TT^CGAA
SmaI         2301  CCCGGG               CCC^GGG
SpeI          749  ACTAGT               A^CTAGT
SphI         3356  GCATGC               GCATG^C
Sse8387I     1732  CCTGCAGG             CCTGCA^GG
SspBI        4982  TGTACA               T^GTACA
SwaI         2034  ATTTAAAT             ATTT^AAAT
XmaCI        2301  CCCGGG               C^CCGGG
XmaI         2301  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AclI         2121  AACGTT               AA^CGTT
             4795  AACGTT              
AcyI         2681  GACGCC               G[GA]^CG[CT]C
             4289  GACGTC              
AflIII       1817  ACATGT               A^C[GA][CT]GT
             4596  ACACGT              
Alw44I        312  GTGCAC               G^TGCAC
             4606  GTGCAC              
AocI         2244  CCTTAGG              CC^T[ACGT]AGG
             3658  CCTGAGG             
ApaLI         312  GTGCAC               G^TGCAC
             4606  GTGCAC              
BalI          446  TGGCCA               TGG^CCA
             3943  TGGCCA              
BanI         1369  GGCACC               G^G[CT][GA]CC
             3832  GGTACC              
BanII         347  GGGCTC               G[GA]GC[CT]^C
             3025  GGGCTC              
BciVI        1485  GGATAC               GTATCC(6/5)
             2889  GTATCC              
BclI         2322  TGATCA               T^GATCA
             2364  TGATCA              
BsaAI        4580  CACGTG               [CT]AC^GT[GA]
             4597  CACGTA              
BsaHI        2681  GACGCC               G[GA]^CG[CT]C
             4289  GACGTC              
BsaWI        2892  TCCGGT               [AT]^CCGG[AT]
             4169  ACCGGA              
BspHI        1608  TCATGA               T^CATGA
             3648  TCATGA              
BspMI        3871  ACCTGC               ACCTGC(4/8)
             2446  GCAGGT              
Bsu36I       2244  CCTTAGG              CC^T[ACGT]AGG
             3658  CCTGAGG             
CfoI            2  GCGC                 GCG^C
             1553  GCGC                
Eco81I       2244  CCTTAGG              CC^T[ACGT]AGG
             3658  CCTGAGG             
EcoRI        4096  GAATTC               G^AATTC
             4276  GAATTC              
FauI          948  CCCGC                CCCGC(4/6)
             3640  GCGGG               
FbaI         2322  TGATCA               T^GATCA
             2364  TGATCA              
HhaI            2  GCGC                 GCG^C
             1553  GCGC                
Hin1I        2681  GACGCC               G[GA]^CG[CT]C
             4289  GACGTC              
HinP1I          2  GCGC                 G^CGC
             1553  GCGC                
MluNI         446  TGGCCA               TGG^CCA
             3943  TGGCCA              
MscI          446  TGGCCA               TGG^CCA
             3943  TGGCCA              
PflMI         860  CCAGTACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1052  CCAGTCCTTGG         
Psp1406I     2121  AACGTT               AA^CGTT
             4795  AACGTT              
RcaI         1608  TCATGA               T^CATGA
             3648  TCATGA              
SapI          517  GAAGAGC              GCTCTTC(1/4)
             2426  GAAGAGC             
StuI         1896  AGGCCT               AGG^CCT
             2533  AGGCCT              
Van91I        860  CCAGTACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1052  CCAGTCCTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaII       AvrII       
BbsI        BbvI        BcnI        BfaI        BlnI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HincII      HindII      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PpuMI       PshAI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII