################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AccIII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp700I AspEI AspI AviII BamHI BbeI BbvCI BglI BlpI Bpu1102I BseAI BsiEI BsiWI BsmI BspDI BspEI BsrBI BssHII BssSI Bst1107I BstEII BstPI BstZ17I CelII ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoT22I FseI FspI HpaI KasI KspI MluI MroI NarI NdeI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmeI PshBI PvuI RsrII SacI SacII SalI ScaI SexAI SfiI SfoI SgrAI SnaBI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 4289 GACGTC GACGT^C Acc65I 3832 GGTACC G^GTACC AccI 1094 GTAGAC GT^[AC][GT]AC AccII 1552 CGCG CG^CG AflII 4207 CTTAAG C^TTAAG Asp718I 3832 GGTACC G^GTACC AvaI 2301 CCCGGG C^[CT]CG[GA]G BbrPI 4580 CACGTG CAC^GTG BcgI 3697 CGATTTCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 4207 CTTAAG C^TTAAG BglII 3336 AGATCT A^GATCT BsaBI 3535 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 2026 GAGACG CGTCTC(1/5) BsoBI 2301 CCCGGG C^[CT]CG[GA]G Bsp1407I 4982 TGTACA T^GTACA BspLU11I 1817 ACATGT A^CATGT BsrFI 3978 GCCGGC [GA]^CCGG[CT] BsrGI 4982 TGTACA T^GTACA BstAPI 2431 GCAGGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 4841 TTCGAA TT^CGAA BstUI 1552 CGCG CG^CG BstXI 3381 CCATGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 3978 GCCGGC [GA]^CCGG[CT] DraI 2035 TTTAAA TTT^AAA DraIII 401 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 4229 GACAGAACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 3381 CCATGG C^C[GA][CT]GG EcoRV 2101 GATATC GAT^ATC HaeII 1 GGCGCT [GA]GCGC^[CT] KpnI 3832 GGTACC GGTAC^C MamI 3535 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 985 CAATTG C^AATTG MunI 985 CAATTG C^AATTG MvnI 1552 CGCG CG^CG NaeI 3978 GCCGGC GCC^GGC NcoI 3381 CCATGG C^CATGG NgoMIV 3978 GCCGGC G^CCGGC NheI 3766 GCTAGC G^CTAGC NspV 4841 TTCGAA TT^CGAA PmaCI 4580 CACGTG CAC^GTG PmlI 4580 CACGTG CAC^GTG SbfI 1732 CCTGCAGG CCTGCA^GG SfuI 4841 TTCGAA TT^CGAA SmaI 2301 CCCGGG CCC^GGG SpeI 749 ACTAGT A^CTAGT SphI 3356 GCATGC GCATG^C Sse8387I 1732 CCTGCAGG CCTGCA^GG SspBI 4982 TGTACA T^GTACA SwaI 2034 ATTTAAAT ATTT^AAAT XmaCI 2301 CCCGGG C^CCGGG XmaI 2301 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AclI 2121 AACGTT AA^CGTT 4795 AACGTT AcyI 2681 GACGCC G[GA]^CG[CT]C 4289 GACGTC AflIII 1817 ACATGT A^C[GA][CT]GT 4596 ACACGT Alw44I 312 GTGCAC G^TGCAC 4606 GTGCAC AocI 2244 CCTTAGG CC^T[ACGT]AGG 3658 CCTGAGG ApaLI 312 GTGCAC G^TGCAC 4606 GTGCAC BalI 446 TGGCCA TGG^CCA 3943 TGGCCA BanI 1369 GGCACC G^G[CT][GA]CC 3832 GGTACC BanII 347 GGGCTC G[GA]GC[CT]^C 3025 GGGCTC BciVI 1485 GGATAC GTATCC(6/5) 2889 GTATCC BclI 2322 TGATCA T^GATCA 2364 TGATCA BsaAI 4580 CACGTG [CT]AC^GT[GA] 4597 CACGTA BsaHI 2681 GACGCC G[GA]^CG[CT]C 4289 GACGTC BsaWI 2892 TCCGGT [AT]^CCGG[AT] 4169 ACCGGA BspHI 1608 TCATGA T^CATGA 3648 TCATGA BspMI 3871 ACCTGC ACCTGC(4/8) 2446 GCAGGT Bsu36I 2244 CCTTAGG CC^T[ACGT]AGG 3658 CCTGAGG CfoI 2 GCGC GCG^C 1553 GCGC Eco81I 2244 CCTTAGG CC^T[ACGT]AGG 3658 CCTGAGG EcoRI 4096 GAATTC G^AATTC 4276 GAATTC FauI 948 CCCGC CCCGC(4/6) 3640 GCGGG FbaI 2322 TGATCA T^GATCA 2364 TGATCA HhaI 2 GCGC GCG^C 1553 GCGC Hin1I 2681 GACGCC G[GA]^CG[CT]C 4289 GACGTC HinP1I 2 GCGC G^CGC 1553 GCGC MluNI 446 TGGCCA TGG^CCA 3943 TGGCCA MscI 446 TGGCCA TGG^CCA 3943 TGGCCA PflMI 860 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1052 CCAGTCCTTGG Psp1406I 2121 AACGTT AA^CGTT 4795 AACGTT RcaI 1608 TCATGA T^CATGA 3648 TCATGA SapI 517 GAAGAGC GCTCTTC(1/4) 2426 GAAGAGC StuI 1896 AGGCCT AGG^CCT 2533 AGGCCT Van91I 860 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1052 CCAGTCCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII AvrII BbsI BbvI BcnI BfaI BlnI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII