##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AccIII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp700I AspEI
AspI AviII BamHI BbeI BbvCI
BglI BlpI Bpu1102I BseAI BsiEI
BsiWI BsmI BspDI BspEI BsrBI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I CelII ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoT22I FseI FspI HpaI KasI
KspI MluI MroI NarI NdeI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmeI PshBI PvuI
RsrII SacI SacII SalI ScaI
SexAI SfiI SfoI SgrAI SnaBI
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 4289 GACGTC GACGT^C
Acc65I 3832 GGTACC G^GTACC
AccI 1094 GTAGAC GT^[AC][GT]AC
AccII 1552 CGCG CG^CG
AflII 4207 CTTAAG C^TTAAG
Asp718I 3832 GGTACC G^GTACC
AvaI 2301 CCCGGG C^[CT]CG[GA]G
BbrPI 4580 CACGTG CAC^GTG
BcgI 3697 CGATTTCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4207 CTTAAG C^TTAAG
BglII 3336 AGATCT A^GATCT
BsaBI 3535 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2026 GAGACG CGTCTC(1/5)
BsoBI 2301 CCCGGG C^[CT]CG[GA]G
Bsp1407I 4982 TGTACA T^GTACA
BspLU11I 1817 ACATGT A^CATGT
BsrFI 3978 GCCGGC [GA]^CCGG[CT]
BsrGI 4982 TGTACA T^GTACA
BstAPI 2431 GCAGGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4841 TTCGAA TT^CGAA
BstUI 1552 CGCG CG^CG
BstXI 3381 CCATGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 3978 GCCGGC [GA]^CCGG[CT]
DraI 2035 TTTAAA TTT^AAA
DraIII 401 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4229 GACAGAACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3381 CCATGG C^C[GA][CT]GG
EcoRV 2101 GATATC GAT^ATC
HaeII 1 GGCGCT [GA]GCGC^[CT]
KpnI 3832 GGTACC GGTAC^C
MamI 3535 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 985 CAATTG C^AATTG
MunI 985 CAATTG C^AATTG
MvnI 1552 CGCG CG^CG
NaeI 3978 GCCGGC GCC^GGC
NcoI 3381 CCATGG C^CATGG
NgoMIV 3978 GCCGGC G^CCGGC
NheI 3766 GCTAGC G^CTAGC
NspV 4841 TTCGAA TT^CGAA
PmaCI 4580 CACGTG CAC^GTG
PmlI 4580 CACGTG CAC^GTG
SbfI 1732 CCTGCAGG CCTGCA^GG
SfuI 4841 TTCGAA TT^CGAA
SmaI 2301 CCCGGG CCC^GGG
SpeI 749 ACTAGT A^CTAGT
SphI 3356 GCATGC GCATG^C
Sse8387I 1732 CCTGCAGG CCTGCA^GG
SspBI 4982 TGTACA T^GTACA
SwaI 2034 ATTTAAAT ATTT^AAAT
XmaCI 2301 CCCGGG C^CCGGG
XmaI 2301 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AclI 2121 AACGTT AA^CGTT
4795 AACGTT
AcyI 2681 GACGCC G[GA]^CG[CT]C
4289 GACGTC
AflIII 1817 ACATGT A^C[GA][CT]GT
4596 ACACGT
Alw44I 312 GTGCAC G^TGCAC
4606 GTGCAC
AocI 2244 CCTTAGG CC^T[ACGT]AGG
3658 CCTGAGG
ApaLI 312 GTGCAC G^TGCAC
4606 GTGCAC
BalI 446 TGGCCA TGG^CCA
3943 TGGCCA
BanI 1369 GGCACC G^G[CT][GA]CC
3832 GGTACC
BanII 347 GGGCTC G[GA]GC[CT]^C
3025 GGGCTC
BciVI 1485 GGATAC GTATCC(6/5)
2889 GTATCC
BclI 2322 TGATCA T^GATCA
2364 TGATCA
BsaAI 4580 CACGTG [CT]AC^GT[GA]
4597 CACGTA
BsaHI 2681 GACGCC G[GA]^CG[CT]C
4289 GACGTC
BsaWI 2892 TCCGGT [AT]^CCGG[AT]
4169 ACCGGA
BspHI 1608 TCATGA T^CATGA
3648 TCATGA
BspMI 3871 ACCTGC ACCTGC(4/8)
2446 GCAGGT
Bsu36I 2244 CCTTAGG CC^T[ACGT]AGG
3658 CCTGAGG
CfoI 2 GCGC GCG^C
1553 GCGC
Eco81I 2244 CCTTAGG CC^T[ACGT]AGG
3658 CCTGAGG
EcoRI 4096 GAATTC G^AATTC
4276 GAATTC
FauI 948 CCCGC CCCGC(4/6)
3640 GCGGG
FbaI 2322 TGATCA T^GATCA
2364 TGATCA
HhaI 2 GCGC GCG^C
1553 GCGC
Hin1I 2681 GACGCC G[GA]^CG[CT]C
4289 GACGTC
HinP1I 2 GCGC G^CGC
1553 GCGC
MluNI 446 TGGCCA TGG^CCA
3943 TGGCCA
MscI 446 TGGCCA TGG^CCA
3943 TGGCCA
PflMI 860 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1052 CCAGTCCTTGG
Psp1406I 2121 AACGTT AA^CGTT
4795 AACGTT
RcaI 1608 TCATGA T^CATGA
3648 TCATGA
SapI 517 GAAGAGC GCTCTTC(1/4)
2426 GAAGAGC
StuI 1896 AGGCCT AGG^CCT
2533 AGGCCT
Van91I 860 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1052 CCAGTCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII AvrII
BbsI BbvI BcnI BfaI BlnI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PshAI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII