##################################
# NO CUT ENZYMES (   64 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        AhdI        
AscI        AseI        AsnI        Asp718I     AspEI       
AvrII       BamHI       BbvCI       BglI        BlnI        
BsiWI       BsmI        Bsp1407I    BspDI       BsrGI       
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
DraI        Eam1105I    EcoT22I     FseI        HindIII     
HpaI        KpnI        KspI        MfeI        MluI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PinAI       PmeI        
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SphI        
Sse8387I    SspBI       SspI        SwaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccIII        815  TCCGGA               T^CCGGA
AflII        2930  CTTAAG               C^TTAAG
Aor51HI       218  AGCGCT               AGC^GCT
ApaI           10  GGGCCC               GGGCC^C
AspI         2459  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         2088  TGGCCA               TGG^CCA
BciVI        3129  GGATAC               GTATCC(6/5)
BfrI         2930  CTTAAG               C^TTAAG
BsaI         1751  GGTCTC               GGTCTC(1/5)
BsaWI         815  TCCGGA               [AT]^CCGG[AT]
BseAI         815  TCCGGA               T^CCGGA
BsiEI        3115  CGGCCG               CG[GA][CT]^CG
BsmBI          80  GAGACG               CGTCTC(1/5)
BspEI         815  TCCGGA               T^CCGGA
BspLU11I     3692  ACATGT               A^CATGT
BsrDI        3435  GCAATG               GCAATG(2/0)
BsrFI          54  GCCGGC               [GA]^CCGG[CT]
BsrI         3557  CCAGT                ACTGG(1/-1)
BstEII       2069  GGTCACC              G^GT[ACGT]ACC
BstPI        2069  GGTCACC              G^GT[ACGT]ACC
Cfr10I         54  GCCGGC               [GA]^CCGG[CT]
DrdI         1339  GACATCCACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         3115  CGGCCG               C^GGCCG
EclXI        3115  CGGCCG               C^GGCCG
Eco47III      218  AGCGCT               AGC^GCT
Eco52I       3115  CGGCCG               C^GGCCG
EcoO65I      2069  GGTCACC              G^GT[ACGT]ACC
EcoRI        1567  GAATTC               G^AATTC
EcoRV         268  GATATC               GAT^ATC
HgaI         1630  GACGC                GACGC(5/10)
MluNI        2088  TGGCCA               TGG^CCA
MroI          815  TCCGGA               T^CCGGA
MscI         2088  TGGCCA               TGG^CCA
NaeI           54  GCCGGC               GCC^GGC
NcoI          128  CCATGG               C^CATGG
NgoMIV         54  GCCGGC               G^CCGGC
PaeR7I       2303  CTCGAG               C^TCGAG
PflFI        2459  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SmaI           28  CCCGGG               CCC^GGG
SpeI         1395  ACTAGT               A^CTAGT
StuI         2412  AGGCCT               AGG^CCT
Tth111I      2459  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         2420  TCTAGA               T^CTAGA
XhoI         2303  CTCGAG               C^TCGAG
XmaCI          28  CCCGGG               C^CCGGG
XmaI           28  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI         1348  GTCTAC               GT^[AC][GT]AC
             2246  GTCTAC              
AflIII       1458  ACGTGT               A^C[GA][CT]GT
             3692  ACATGT              
AviII        1607  TGCGCA               TGC^GCA
             3294  TGCGCA              
BbeI          407  GGCGCC               GGCGC^C
             3383  GGCGCC              
BcgI          638  GCACAGGCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2878  GCAGTTTTCTCG        
BclI          473  TGATCA               T^GATCA
              629  TGATCA              
BglII        1214  AGATCT               A^GATCT
             1655  AGATCT              
BsaBI         483  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              813  GATCCGGATC          
BseRI        2416  CTCCTC               GAGGAG(10/8)
             2844  CTCCTC              
BsrBI         250  CCGCTC               CCGCTC(-3/-3)
               68  GAGCGG              
BssHII        158  GCGCGC               G^CGCGC
              285  GCGCGC              
BssSI         328  CACGAG               CACGAG(-5/-1)
             2949  CTCGTG              
BstXI         660  CCACAAGTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1698  CCAGCTGCATGG        
DsaI          128  CCATGG               C^C[GA][CT]GG
             3724  CCGTGG              
EcoNI         948  CCTGGATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1484  CCTGCATGAGG         
FbaI          473  TGATCA               T^GATCA
              629  TGATCA              
FspI         1607  TGCGCA               TGC^GCA
             3294  TGCGCA              
HincII       3347  GTCAAC               GT[CT]^[GA]AC
             3703  GTCAAC              
HindII       3347  GTCAAC               GT[CT]^[GA]AC
             3703  GTCAAC              
KasI          407  GGCGCC               G^GCGCC
             3383  GGCGCC              
MamI          483  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              813  GATCCGGATC          
NarI          407  GGCGCC               GG^CGCC
             3383  GGCGCC              
PshAI         969  GACAGGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1841  GACCTAAGTC          
PstI         2876  CTGCAG               CTGCA^G
             3334  CTGCAG              
SfcI         2876  CTGCAG               C^T[GA][CT]AG
             3334  CTGCAG              
SfoI          407  GGCGCC               GGC^GCC
             3383  GGCGCC              
XcmI         2820  CCAGAGCTGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3644  CCAACCACACGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        Alw44I      AlwI        AlwNI       AocI        
ApaLI       ApoI        Asp700I     AspHI       AvaI        
AvaII       BanI        BanII       BbrPI       BbsI        
BbvI        BcnI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaJI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspHI       
BspMI       BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstYI       Bsu36I      Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DraIII      EaeI        EarI        Eco81I      EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PmaCI       PmlI        PpuMI       
PvuII       RcaI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII       
XmnI