##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AscI AseI AsnI Asp718I AspEI
AvrII BamHI BbvCI BglI BlnI
BsiWI BsmI Bsp1407I BspDI BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI Eam1105I EcoT22I FseI HindIII
HpaI KpnI KspI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SphI
Sse8387I SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 815 TCCGGA T^CCGGA
AflII 2930 CTTAAG C^TTAAG
Aor51HI 218 AGCGCT AGC^GCT
ApaI 10 GGGCCC GGGCC^C
AspI 2459 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2088 TGGCCA TGG^CCA
BciVI 3129 GGATAC GTATCC(6/5)
BfrI 2930 CTTAAG C^TTAAG
BsaI 1751 GGTCTC GGTCTC(1/5)
BsaWI 815 TCCGGA [AT]^CCGG[AT]
BseAI 815 TCCGGA T^CCGGA
BsiEI 3115 CGGCCG CG[GA][CT]^CG
BsmBI 80 GAGACG CGTCTC(1/5)
BspEI 815 TCCGGA T^CCGGA
BspLU11I 3692 ACATGT A^CATGT
BsrDI 3435 GCAATG GCAATG(2/0)
BsrFI 54 GCCGGC [GA]^CCGG[CT]
BsrI 3557 CCAGT ACTGG(1/-1)
BstEII 2069 GGTCACC G^GT[ACGT]ACC
BstPI 2069 GGTCACC G^GT[ACGT]ACC
Cfr10I 54 GCCGGC [GA]^CCGG[CT]
DrdI 1339 GACATCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3115 CGGCCG C^GGCCG
EclXI 3115 CGGCCG C^GGCCG
Eco47III 218 AGCGCT AGC^GCT
Eco52I 3115 CGGCCG C^GGCCG
EcoO65I 2069 GGTCACC G^GT[ACGT]ACC
EcoRI 1567 GAATTC G^AATTC
EcoRV 268 GATATC GAT^ATC
HgaI 1630 GACGC GACGC(5/10)
MluNI 2088 TGGCCA TGG^CCA
MroI 815 TCCGGA T^CCGGA
MscI 2088 TGGCCA TGG^CCA
NaeI 54 GCCGGC GCC^GGC
NcoI 128 CCATGG C^CATGG
NgoMIV 54 GCCGGC G^CCGGC
PaeR7I 2303 CTCGAG C^TCGAG
PflFI 2459 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SmaI 28 CCCGGG CCC^GGG
SpeI 1395 ACTAGT A^CTAGT
StuI 2412 AGGCCT AGG^CCT
Tth111I 2459 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2420 TCTAGA T^CTAGA
XhoI 2303 CTCGAG C^TCGAG
XmaCI 28 CCCGGG C^CCGGG
XmaI 28 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1348 GTCTAC GT^[AC][GT]AC
2246 GTCTAC
AflIII 1458 ACGTGT A^C[GA][CT]GT
3692 ACATGT
AviII 1607 TGCGCA TGC^GCA
3294 TGCGCA
BbeI 407 GGCGCC GGCGC^C
3383 GGCGCC
BcgI 638 GCACAGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2878 GCAGTTTTCTCG
BclI 473 TGATCA T^GATCA
629 TGATCA
BglII 1214 AGATCT A^GATCT
1655 AGATCT
BsaBI 483 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
813 GATCCGGATC
BseRI 2416 CTCCTC GAGGAG(10/8)
2844 CTCCTC
BsrBI 250 CCGCTC CCGCTC(-3/-3)
68 GAGCGG
BssHII 158 GCGCGC G^CGCGC
285 GCGCGC
BssSI 328 CACGAG CACGAG(-5/-1)
2949 CTCGTG
BstXI 660 CCACAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1698 CCAGCTGCATGG
DsaI 128 CCATGG C^C[GA][CT]GG
3724 CCGTGG
EcoNI 948 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1484 CCTGCATGAGG
FbaI 473 TGATCA T^GATCA
629 TGATCA
FspI 1607 TGCGCA TGC^GCA
3294 TGCGCA
HincII 3347 GTCAAC GT[CT]^[GA]AC
3703 GTCAAC
HindII 3347 GTCAAC GT[CT]^[GA]AC
3703 GTCAAC
KasI 407 GGCGCC G^GCGCC
3383 GGCGCC
MamI 483 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
813 GATCCGGATC
NarI 407 GGCGCC GG^CGCC
3383 GGCGCC
PshAI 969 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1841 GACCTAAGTC
PstI 2876 CTGCAG CTGCA^G
3334 CTGCAG
SfcI 2876 CTGCAG C^T[GA][CT]AG
3334 CTGCAG
SfoI 407 GGCGCC GGC^GCC
3383 GGCGCC
XcmI 2820 CCAGAGCTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3644 CCAACCACACGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbrPI BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspMI BssKI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DraIII EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI