################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII Acc65I AclI AgeI AhdI AscI AseI AsnI Asp718I AspEI AvrII BamHI BbvCI BglI BlnI BsiWI BsmI Bsp1407I BspDI BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DraI Eam1105I EcoT22I FseI HindIII HpaI KpnI KspI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SphI Sse8387I SspBI SspI SwaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccIII 815 TCCGGA T^CCGGA AflII 2930 CTTAAG C^TTAAG Aor51HI 218 AGCGCT AGC^GCT ApaI 10 GGGCCC GGGCC^C AspI 2459 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2088 TGGCCA TGG^CCA BciVI 3129 GGATAC GTATCC(6/5) BfrI 2930 CTTAAG C^TTAAG BsaI 1751 GGTCTC GGTCTC(1/5) BsaWI 815 TCCGGA [AT]^CCGG[AT] BseAI 815 TCCGGA T^CCGGA BsiEI 3115 CGGCCG CG[GA][CT]^CG BsmBI 80 GAGACG CGTCTC(1/5) BspEI 815 TCCGGA T^CCGGA BspLU11I 3692 ACATGT A^CATGT BsrDI 3435 GCAATG GCAATG(2/0) BsrFI 54 GCCGGC [GA]^CCGG[CT] BsrI 3557 CCAGT ACTGG(1/-1) BstEII 2069 GGTCACC G^GT[ACGT]ACC BstPI 2069 GGTCACC G^GT[ACGT]ACC Cfr10I 54 GCCGGC [GA]^CCGG[CT] DrdI 1339 GACATCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 3115 CGGCCG C^GGCCG EclXI 3115 CGGCCG C^GGCCG Eco47III 218 AGCGCT AGC^GCT Eco52I 3115 CGGCCG C^GGCCG EcoO65I 2069 GGTCACC G^GT[ACGT]ACC EcoRI 1567 GAATTC G^AATTC EcoRV 268 GATATC GAT^ATC HgaI 1630 GACGC GACGC(5/10) MluNI 2088 TGGCCA TGG^CCA MroI 815 TCCGGA T^CCGGA MscI 2088 TGGCCA TGG^CCA NaeI 54 GCCGGC GCC^GGC NcoI 128 CCATGG C^CATGG NgoMIV 54 GCCGGC G^CCGGC PaeR7I 2303 CTCGAG C^TCGAG PflFI 2459 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC SmaI 28 CCCGGG CCC^GGG SpeI 1395 ACTAGT A^CTAGT StuI 2412 AGGCCT AGG^CCT Tth111I 2459 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2420 TCTAGA T^CTAGA XhoI 2303 CTCGAG C^TCGAG XmaCI 28 CCCGGG C^CCGGG XmaI 28 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 1348 GTCTAC GT^[AC][GT]AC 2246 GTCTAC AflIII 1458 ACGTGT A^C[GA][CT]GT 3692 ACATGT AviII 1607 TGCGCA TGC^GCA 3294 TGCGCA BbeI 407 GGCGCC GGCGC^C 3383 GGCGCC BcgI 638 GCACAGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2878 GCAGTTTTCTCG BclI 473 TGATCA T^GATCA 629 TGATCA BglII 1214 AGATCT A^GATCT 1655 AGATCT BsaBI 483 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 813 GATCCGGATC BseRI 2416 CTCCTC GAGGAG(10/8) 2844 CTCCTC BsrBI 250 CCGCTC CCGCTC(-3/-3) 68 GAGCGG BssHII 158 GCGCGC G^CGCGC 285 GCGCGC BssSI 328 CACGAG CACGAG(-5/-1) 2949 CTCGTG BstXI 660 CCACAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1698 CCAGCTGCATGG DsaI 128 CCATGG C^C[GA][CT]GG 3724 CCGTGG EcoNI 948 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1484 CCTGCATGAGG FbaI 473 TGATCA T^GATCA 629 TGATCA FspI 1607 TGCGCA TGC^GCA 3294 TGCGCA HincII 3347 GTCAAC GT[CT]^[GA]AC 3703 GTCAAC HindII 3347 GTCAAC GT[CT]^[GA]AC 3703 GTCAAC KasI 407 GGCGCC G^GCGCC 3383 GGCGCC MamI 483 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 813 GATCCGGATC NarI 407 GGCGCC GG^CGCC 3383 GGCGCC PshAI 969 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1841 GACCTAAGTC PstI 2876 CTGCAG CTGCA^G 3334 CTGCAG SfcI 2876 CTGCAG C^T[GA][CT]AG 3334 CTGCAG SfoI 407 GGCGCC GGC^GCC 3383 GGCGCC XcmI 2820 CCAGAGCTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3644 CCAACCACACGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaLI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbrPI BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BssKI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI