##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AclI AgeI AhdI Alw44I
ApaLI AscI Asp700I Asp718I AspEI
AspI AviII AvrII BcgI BglI
BlnI BsiWI BspDI BsrDI BssHII
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoRV FseI FspI HincII
HindII HpaI KpnI MfeI MluI
MunI NotI NruI NspV PacI
PflFI PinAI PmeI Psp1406I PvuI
RsrII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SphI
Sse8387I SspI StuI SwaI Tth111I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 285 GACGTC GACGT^C
AccIII 63 TCCGGA T^CCGGA
AlwNI 1126 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 2447 AGCGCT AGC^GCT
AseI 1389 ATTAAT AT^TAAT
AsnI 1389 ATTAAT AT^TAAT
BbeI 599 GGCGCC GGCGC^C
BbrPI 1977 CACGTG CAC^GTG
BclI 3678 TGATCA T^GATCA
BsaAI 1977 CACGTG [CT]AC^GT[GA]
BseAI 63 TCCGGA T^CCGGA
BsiEI 169 CGACCG CG[GA][CT]^CG
BspEI 63 TCCGGA T^CCGGA
BspHI 3261 TCATGA T^CATGA
BspLU11I 3600 ACATGT A^CATGT
BsrBI 2691 GAGCGG CCGCTC(-3/-3)
BstEII 4297 GGTGACC G^GT[ACGT]ACC
BstPI 4297 GGTGACC G^GT[ACGT]ACC
BstXI 2366 CCAAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 4331 TTTAAA TTT^AAA
DraIII 3324 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 2506 CTCTTC CTCTTC(1/4)
Eco47III 2447 AGCGCT AGC^GCT
EcoO65I 4297 GGTGACC G^GT[ACGT]ACC
EcoRI 4099 GAATTC G^AATTC
EcoT22I 2177 ATGCAT ATGCA^T
FbaI 3678 TGATCA T^GATCA
KasI 599 GGCGCC G^GCGCC
Ksp632I 2506 CTCTTC CTCTTC(1/4)
MroI 63 TCCGGA T^CCGGA
NaeI 2990 GCCGGC GCC^GGC
NarI 599 GGCGCC GG^CGCC
NdeI 1207 CATATG CA^TATG
NgoMIV 2990 GCCGGC G^CCGGC
NheI 662 GCTAGC G^CTAGC
NsiI 2177 ATGCAT ATGCA^T
PaeR7I 3842 CTCGAG C^TCGAG
PmaCI 1977 CACGTG CAC^GTG
PmlI 1977 CACGTG CAC^GTG
PshAI 270 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1389 ATTAAT AT^TAAT
RcaI 3261 TCATGA T^CATGA
SapI 2505 GCTCTTC GCTCTTC(1/4)
SfoI 599 GGCGCC GGC^GCC
SmaI 2338 CCCGGG CCC^GGG
SpeI 1277 ACTAGT A^CTAGT
XhoI 3842 CTCGAG C^TCGAG
XmaCI 2338 CCCGGG C^CCGGG
XmaI 2338 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 477 GTCTAC GT^[AC][GT]AC
1146 GTAGAC
AflII 2284 CTTAAG C^TTAAG
3192 CTTAAG
AflIII 1978 ACGTGT A^C[GA][CT]GT
3600 ACATGT
AspHI 28 GAGCTC G[AT]GC[AT]^C
1299 GAGCTC
BbvCI 3068 CCTCAGC CCTCAGC(-5/-2)
2607 GCTGAGG
BciVI 343 GTATCC GTATCC(6/5)
3616 GTATCC
BfrI 2284 CTTAAG C^TTAAG
3192 CTTAAG
BglII 1827 AGATCT A^GATCT
2805 AGATCT
BsaBI 2098 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3673 GATGTTGATC
BsiHKAI 28 GAGCTC G[AT]GC[AT]^C
1299 GAGCTC
BsmBI 675 CGTCTC CGTCTC(1/5)
2556 CGTCTC
BsmI 1949 GCATTC GAATGC(1/-1)
2831 GCATTC
Bsp1407I 712 TGTACA T^GTACA
769 TGTACA
BsrFI 37 GCCGGT [GA]^CCGG[CT]
2990 GCCGGC
BsrGI 712 TGTACA T^GTACA
769 TGTACA
BssSI 549 CTCGTG CACGAG(-5/-1)
1894 CTCGTG
Cfr10I 37 GCCGGT [GA]^CCGG[CT]
2990 GCCGGC
EcoNI 1699 CCTCCTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3353 CCTTGCACAGG
HgaI 886 GACGC GACGC(5/10)
674 GCGTC
HindIII 845 AAGCTT A^AGCTT
3151 AAGCTT
KspI 14 CCGCGG CCGC^GG
890 CCGCGG
MamI 2098 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3673 GATGTTGATC
NspI 1260 ACATGC [GA]CATG^[CT]
3600 ACATGT
SacI 28 GAGCTC GAGCT^C
1299 GAGCTC
SacII 14 CCGCGG CCGC^GG
890 CCGCGG
SspBI 712 TGTACA T^GTACA
769 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AocI ApaI ApoI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII