##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BcgI BlnI BsaWI
BseAI BsiWI BspDI BspEI BspHI
BsrBI BssHII Bst1107I BstEII BstPI
BstUI BstZ17I ClaI CpoI DraIII
Eam1105I Eco47III EcoNI EcoO65I EcoT22I
FseI FspI HincII HindII HpaI
KpnI KspI MfeI MluI MroI
MunI MvnI NheI NotI NruI
NsiI PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI RcaI
RsrII SacII SalI SapI SbfI
SfcI SfiI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 2385 GACGTC GACGT^C
AccI 270 GTCTAC GT^[AC][GT]AC
AlwNI 2459 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1549 CCTGAGG CC^T[ACGT]AGG
BbeI 38 GGCGCC GGCGC^C
BbrPI 3536 CACGTG CAC^GTG
BbvCI 1280 CCTCAGC CCTCAGC(-5/-2)
BciVI 3303 GGATAC GTATCC(6/5)
BclI 1745 TGATCA T^GATCA
BglI 30 GCCATGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3280 AGATCT A^GATCT
BsaAI 3536 CACGTG [CT]AC^GT[GA]
BsaBI 1912 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1203 GAGACG CGTCTC(1/5)
BsmFI 2823 GGGAC GGGAC(10/14)
BspLU11I 2433 ACATGT A^CATGT
BsrFI 569 GCCGGC [GA]^CCGG[CT]
BssSI 1100 CTCGTG CACGAG(-5/-1)
BstAPI 3172 GCAACACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 327 CCAGGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 3280 AGATCT [GA]^GATC[CT]
Bsu36I 1549 CCTGAGG CC^T[ACGT]AGG
Cfr10I 569 GCCGGC [GA]^CCGG[CT]
DrdI 83 GACTGCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 31 CCATGG C^C[GA][CT]GG
EagI 571 CGGCCG C^GGCCG
EclXI 571 CGGCCG C^GGCCG
Eco52I 571 CGGCCG C^GGCCG
Eco81I 1549 CCTGAGG CC^T[ACGT]AGG
EcoRI 468 GAATTC G^AATTC
FbaI 1745 TGATCA T^GATCA
HaeII 38 GGCGCC [GA]GCGC^[CT]
HindIII 2677 AAGCTT A^AGCTT
KasI 38 GGCGCC G^GCGCC
MamI 1912 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 3280 AGATCT [GA]^GATC[CT]
NaeI 569 GCCGGC GCC^GGC
NarI 38 GGCGCC GG^CGCC
NcoI 31 CCATGG C^CATGG
NgoMIV 569 GCCGGC G^CCGGC
NspI 2433 ACATGT [GA]CATG^[CT]
PflMI 1810 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3536 CACGTG CAC^GTG
PmlI 3536 CACGTG CAC^GTG
PvuI 3331 CGATCG CGAT^CG
ScaI 1180 AGTACT AGT^ACT
SexAI 599 ACCTGGT A^CC[AT]GGT
SfoI 38 GGCGCC GGC^GCC
StuI 359 AGGCCT AGG^CCT
Van91I 1810 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 3280 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Alw44I 536 GTGCAC G^TGCAC
987 GTGCAC
AlwI 2488 GATCC GGATC(4/5)
1951 GGATC
ApaLI 536 GTGCAC G^TGCAC
987 GTGCAC
Asp700I 346 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3555 GAATTGCTTC
BalI 1478 TGGCCA TGG^CCA
3767 TGGCCA
BanI 38 GGCGCC G^G[CT][GA]CC
1503 GGTGCC
BbvI 16 GCAGC GCAGC(8/12)
835 GCAGC
BcnI 1268 CCCGG CC^[GC]GG
2228 CCCGG
BpmI 1236 CTCCAG CTGGAG(16/14)
1397 CTCCAG
BsaI 1219 GAGACC GGTCTC(1/5)
3812 GGTCTC
BsmI 1085 GCATTC GAATGC(1/-1)
2584 GCATTC
Bsp1407I 727 TGTACA T^GTACA
3572 TGTACA
BsrDI 815 GCAATG GCAATG(2/0)
3167 GCAATG
BsrGI 727 TGTACA T^GTACA
3572 TGTACA
BstBI 683 TTCGAA TT^CGAA
1033 TTCGAA
DraII 1022 AGGACCT [GA]G^G[ACGT]CC[CT]
2823 GGGACCT
EcoO109I 1022 AGGACCT [GA]G^G[ACGT]CC[CT]
2823 GGGACCT
EcoRV 1318 GATATC GAT^ATC
1585 GATATC
FauI 2236 CCCGC CCCGC(4/6)
133 GCGGG
MluNI 1478 TGGCCA TGG^CCA
3767 TGGCCA
MscI 1478 TGGCCA TGG^CCA
3767 TGGCCA
NciI 1268 CCCGG CC^[GC]GG
2228 CCCGG
NdeI 667 CATATG CA^TATG
1600 CATATG
NspV 683 TTCGAA TT^CGAA
1033 TTCGAA
PaeR7I 25 CTCGAG C^TCGAG
2167 CTCGAG
PpuMI 1022 AGGACCT [GA]G^G[AT]CC[CT]
2823 GGGACCT
PvuII 1283 CAGCTG CAG^CTG
1907 CAGCTG
SacI 1147 GAGCTC GAGCT^C
1441 GAGCTC
SfuI 683 TTCGAA TT^CGAA
1033 TTCGAA
SspBI 727 TGTACA T^GTACA
3572 TGTACA
SspI 3725 AATATT AAT^ATT
3775 AATATT
TseI 16 GCAGC G^C[AT]GC
835 GCAGC
XcmI 1421 CCAGGTGGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2401 CCAAAGGAGAGCTGG
XhoI 25 CTCGAG C^TCGAG
2167 CTCGAG
XmnI 346 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3555 GAATTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflII
AflIII AluI ApoI AspHI AvaI
AvaII BanII BbsI BfaI BfrI
BlpI BmyI Bpu1102I BpuAI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TfiI Tru9I
Tsp45I Tsp509I TspRI TthHB8I XbaI