################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI AscI AspEI AspI BbrPI BfrI BsaBI BseAI BsiWI BspDI BspEI BspLU11I BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV HpaI MamI MfeI MluI MroI MunI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I RsrII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 3662 GTCTAC GT^[AC][GT]AC AccII 1667 CGCG CG^CG AcyI 711 GGCGCC G[GA]^CG[CT]C Aor51HI 1791 AGCGCT AGC^GCT ApaI 681 GGGCCC GGGCC^C AseI 4229 ATTAAT AT^TAAT AsnI 4229 ATTAAT AT^TAAT Asp700I 1826 GAATCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 5062 TGCGCA TGC^GCA AvrII 2717 CCTAGG C^CTAGG BamHI 2644 GGATCC G^GATCC BbeI 711 GGCGCC GGCGC^C BbvCI 1296 CCTCAGC CCTCAGC(-5/-2) BcgI 2192 GCAACAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2882 GGATAC GTATCC(6/5) BglII 494 AGATCT A^GATCT BlnI 2717 CCTAGG C^CTAGG BsaHI 711 GGCGCC G[GA]^CG[CT]C BsiEI 200 CGATCG CG[GA][CT]^CG BsrBI 3243 CCGCTC CCGCTC(-3/-3) BssSI 3027 CACGAG CACGAG(-5/-1) BstUI 1667 CGCG CG^CG BstXI 1738 CCAGCCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 4262 TTTAAA TTT^AAA DraIII 453 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 1791 AGCGCT AGC^GCT EcoRI 3823 GAATTC G^AATTC EcoT22I 2466 ATGCAT ATGCA^T FseI 4485 GGCCGGCC GGCCGG^CC FspI 5062 TGCGCA TGC^GCA Hin1I 711 GGCGCC G[GA]^CG[CT]C HincII 1638 GTTGAC GT[CT]^[GA]AC HindII 1638 GTTGAC GT[CT]^[GA]AC KasI 711 GGCGCC G^GCGCC KspI 1666 CCGCGG CCGC^GG MvnI 1667 CGCG CG^CG NarI 711 GGCGCC GG^CGCC NdeI 1227 CATATG CA^TATG NheI 1596 GCTAGC G^CTAGC NsiI 2466 ATGCAT ATGCA^T PshAI 946 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4229 ATTAAT AT^TAAT PvuI 200 CGATCG CGAT^CG SacII 1666 CCGCGG CCGC^GG SexAI 982 ACCTGGT A^CC[AT]GGT SfoI 711 GGCGCC GGC^GCC XbaI 3205 TCTAGA T^CTAGA XcmI 3926 CCAGTCACCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1826 GAATCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### Acc65I 986 GGTACC G^GTACC 2785 GGTACC AcsI 3470 AAATTT [GA]^AATT[CT] 3823 GAATTC AflIII 3087 ACGTGT A^C[GA][CT]GT 4049 ACACGT Alw44I 3971 GTGCAC G^TGCAC 4045 GTGCAC AocI 2816 CCTCAGG CC^T[ACGT]AGG 3605 CCTCAGG ApaLI 3971 GTGCAC G^TGCAC 4045 GTGCAC ApoI 3470 AAATTT [GA]^AATT[CT] 3823 GAATTC Asp718I 986 GGTACC G^GTACC 2785 GGTACC BalI 892 TGGCCA TGG^CCA 3696 TGGCCA BglI 608 GCCAACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4486 GCCGGCCCGGC BsaI 3128 GGTCTC GGTCTC(1/5) 4119 GGTCTC BsaWI 923 ACCGGA [AT]^CCGG[AT] 3684 ACCGGA BsmBI 1468 CGTCTC CGTCTC(1/5) 1483 CGTCTC BsmI 2123 GCATTC GAATGC(1/-1) 2468 GCATTC Bsp1407I 2873 TGTACA T^GTACA 4062 TGTACA BspHI 3400 TCATGA T^CATGA 3948 TCATGA BsrDI 5035 CATTGC GCAATG(2/0) 2754 GCAATG BsrGI 2873 TGTACA T^GTACA 4062 TGTACA Bsu36I 2816 CCTCAGG CC^T[ACGT]AGG 3605 CCTCAGG Eco81I 2816 CCTCAGG CC^T[ACGT]AGG 3605 CCTCAGG FauI 1553 CCCGC CCCGC(4/6) 4889 CCCGC HindIII 4677 AAGCTT A^AGCTT 4834 AAGCTT KpnI 986 GGTACC GGTAC^C 2785 GGTACC MluNI 892 TGGCCA TGG^CCA 3696 TGGCCA MscI 892 TGGCCA TGG^CCA 3696 TGGCCA PflMI 3407 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4464 CCAAAGTCTGG RcaI 3400 TCATGA T^CATGA 3948 TCATGA SapI 405 GAAGAGC GCTCTTC(1/4) 4946 GAAGAGC SmaI 964 CCCGGG CCC^GGG 2632 CCCGGG SphI 247 GCATGC GCATG^C 728 GCATGC SspBI 2873 TGTACA T^GTACA 4062 TGTACA StuI 1093 AGGCCT AGG^CCT 3038 AGGCCT Van91I 3407 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4464 CCAAAGTCTGG XmaCI 964 CCCGGG C^CCGGG 2632 CCCGGG XmaI 964 CCCGGG C^CCGGG 2632 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbsI BbvI BclI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII