##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AscI AspEI AspI BbrPI
BfrI BsaBI BseAI BsiWI BspDI
BspEI BspLU11I BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRV HpaI MamI MfeI
MluI MroI MunI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I RsrII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 3662 GTCTAC GT^[AC][GT]AC
AccII 1667 CGCG CG^CG
AcyI 711 GGCGCC G[GA]^CG[CT]C
Aor51HI 1791 AGCGCT AGC^GCT
ApaI 681 GGGCCC GGGCC^C
AseI 4229 ATTAAT AT^TAAT
AsnI 4229 ATTAAT AT^TAAT
Asp700I 1826 GAATCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 5062 TGCGCA TGC^GCA
AvrII 2717 CCTAGG C^CTAGG
BamHI 2644 GGATCC G^GATCC
BbeI 711 GGCGCC GGCGC^C
BbvCI 1296 CCTCAGC CCTCAGC(-5/-2)
BcgI 2192 GCAACAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2882 GGATAC GTATCC(6/5)
BglII 494 AGATCT A^GATCT
BlnI 2717 CCTAGG C^CTAGG
BsaHI 711 GGCGCC G[GA]^CG[CT]C
BsiEI 200 CGATCG CG[GA][CT]^CG
BsrBI 3243 CCGCTC CCGCTC(-3/-3)
BssSI 3027 CACGAG CACGAG(-5/-1)
BstUI 1667 CGCG CG^CG
BstXI 1738 CCAGCCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 4262 TTTAAA TTT^AAA
DraIII 453 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1791 AGCGCT AGC^GCT
EcoRI 3823 GAATTC G^AATTC
EcoT22I 2466 ATGCAT ATGCA^T
FseI 4485 GGCCGGCC GGCCGG^CC
FspI 5062 TGCGCA TGC^GCA
Hin1I 711 GGCGCC G[GA]^CG[CT]C
HincII 1638 GTTGAC GT[CT]^[GA]AC
HindII 1638 GTTGAC GT[CT]^[GA]AC
KasI 711 GGCGCC G^GCGCC
KspI 1666 CCGCGG CCGC^GG
MvnI 1667 CGCG CG^CG
NarI 711 GGCGCC GG^CGCC
NdeI 1227 CATATG CA^TATG
NheI 1596 GCTAGC G^CTAGC
NsiI 2466 ATGCAT ATGCA^T
PshAI 946 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4229 ATTAAT AT^TAAT
PvuI 200 CGATCG CGAT^CG
SacII 1666 CCGCGG CCGC^GG
SexAI 982 ACCTGGT A^CC[AT]GGT
SfoI 711 GGCGCC GGC^GCC
XbaI 3205 TCTAGA T^CTAGA
XcmI 3926 CCAGTCACCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1826 GAATCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 986 GGTACC G^GTACC
2785 GGTACC
AcsI 3470 AAATTT [GA]^AATT[CT]
3823 GAATTC
AflIII 3087 ACGTGT A^C[GA][CT]GT
4049 ACACGT
Alw44I 3971 GTGCAC G^TGCAC
4045 GTGCAC
AocI 2816 CCTCAGG CC^T[ACGT]AGG
3605 CCTCAGG
ApaLI 3971 GTGCAC G^TGCAC
4045 GTGCAC
ApoI 3470 AAATTT [GA]^AATT[CT]
3823 GAATTC
Asp718I 986 GGTACC G^GTACC
2785 GGTACC
BalI 892 TGGCCA TGG^CCA
3696 TGGCCA
BglI 608 GCCAACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4486 GCCGGCCCGGC
BsaI 3128 GGTCTC GGTCTC(1/5)
4119 GGTCTC
BsaWI 923 ACCGGA [AT]^CCGG[AT]
3684 ACCGGA
BsmBI 1468 CGTCTC CGTCTC(1/5)
1483 CGTCTC
BsmI 2123 GCATTC GAATGC(1/-1)
2468 GCATTC
Bsp1407I 2873 TGTACA T^GTACA
4062 TGTACA
BspHI 3400 TCATGA T^CATGA
3948 TCATGA
BsrDI 5035 CATTGC GCAATG(2/0)
2754 GCAATG
BsrGI 2873 TGTACA T^GTACA
4062 TGTACA
Bsu36I 2816 CCTCAGG CC^T[ACGT]AGG
3605 CCTCAGG
Eco81I 2816 CCTCAGG CC^T[ACGT]AGG
3605 CCTCAGG
FauI 1553 CCCGC CCCGC(4/6)
4889 CCCGC
HindIII 4677 AAGCTT A^AGCTT
4834 AAGCTT
KpnI 986 GGTACC GGTAC^C
2785 GGTACC
MluNI 892 TGGCCA TGG^CCA
3696 TGGCCA
MscI 892 TGGCCA TGG^CCA
3696 TGGCCA
PflMI 3407 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4464 CCAAAGTCTGG
RcaI 3400 TCATGA T^CATGA
3948 TCATGA
SapI 405 GAAGAGC GCTCTTC(1/4)
4946 GAAGAGC
SmaI 964 CCCGGG CCC^GGG
2632 CCCGGG
SphI 247 GCATGC GCATG^C
728 GCATGC
SspBI 2873 TGTACA T^GTACA
4062 TGTACA
StuI 1093 AGGCCT AGG^CCT
3038 AGGCCT
Van91I 3407 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4464 CCAAAGTCTGG
XmaCI 964 CCCGGG C^CCGGG
2632 CCCGGG
XmaI 964 CCCGGG C^CCGGG
2632 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BclI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII