##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccI AclI AflII AflIII
AhdI AscI AseI AsnI Asp700I
AspEI AspI BbeI BbsI BbvCI
BciVI BclI BfrI BpuAI BsiWI
Bsp1407I BspHI BspLU11I BsrGI Bst1107I
BstBI BstZ17I CpoI DraIII Eam1105I
EcoRV FbaI FseI HindIII HpaI
KasI KspI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PmeI PshAI PshBI Psp1406I RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 3157 TCCGGA T^CCGGA
AcsI 2529 GAATTC [GA]^AATT[CT]
AgeI 976 ACCGGT A^CCGGT
ApoI 2529 GAATTC [GA]^AATT[CT]
AvrII 2408 CCTAGG C^CTAGG
BamHI 2779 GGATCC G^GATCC
BbrPI 1847 CACGTG CAC^GTG
BcgI 2056 CGATCCGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1552 AGATCT A^GATCT
BlnI 2408 CCTAGG C^CTAGG
BsaAI 1847 CACGTG [CT]AC^GT[GA]
BseAI 3157 TCCGGA T^CCGGA
BsmBI 3418 CGTCTC CGTCTC(1/5)
BsmI 1535 GCATTC GAATGC(1/-1)
BspDI 3786 ATCGAT AT^CGAT
BspEI 3157 TCCGGA T^CCGGA
BstEII 3709 GGTGACC G^GT[ACGT]ACC
BstPI 3709 GGTGACC G^GT[ACGT]ACC
BstXI 3664 CCAAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 3786 ATCGAT AT^CGAT
DraI 3026 TTTAAA TTT^AAA
DrdI 3484 GACTTCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1760 GAAGAG CTCTTC(1/4)
EcoO65I 3709 GGTGACC G^GT[ACGT]ACC
EcoRI 2529 GAATTC G^AATTC
Ksp632I 1760 GAAGAG CTCTTC(1/4)
MroI 3157 TCCGGA T^CCGGA
MslI 3699 CACAAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 1667 GCTAGC G^CTAGC
NspI 2852 GCATGT [GA]CATG^[CT]
PinAI 976 ACCGGT A^CCGGT
PmaCI 1847 CACGTG CAC^GTG
PmlI 1847 CACGTG CAC^GTG
PvuI 2281 CGATCG CGAT^CG
SacI 1546 GAGCTC GAGCT^C
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 1802 GGTACC G^GTACC
2396 GGTACC
Alw44I 785 GTGCAC G^TGCAC
1191 GTGCAC
Aor51HI 2807 AGCGCT AGC^GCT
3152 AGCGCT
ApaI 125 GGGCCC GGGCC^C
1648 GGGCCC
ApaLI 785 GTGCAC G^TGCAC
1191 GTGCAC
Asp718I 1802 GGTACC G^GTACC
2396 GGTACC
BglI 1163 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1684 GCCGGGGAGGC
BlpI 2355 GCTGAGC GC^T[ACGT]AGC
3449 GCTCAGC
Bpu1102I 2355 GCTGAGC GC^T[ACGT]AGC
3449 GCTCAGC
BsaBI 2051 GATTTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2904 GATCCAAATC
BsrDI 896 CATTGC GCAATG(2/0)
931 GCAATG
CelII 2355 GCTGAGC GC^T[ACGT]AGC
3449 GCTCAGC
EagI 1682 CGGCCG C^GGCCG
2046 CGGCCG
EclXI 1682 CGGCCG C^GGCCG
2046 CGGCCG
Eco47III 2807 AGCGCT AGC^GCT
3152 AGCGCT
Eco52I 1682 CGGCCG C^GGCCG
2046 CGGCCG
EcoT22I 1533 ATGCAT ATGCA^T
2850 ATGCAT
KpnI 1802 GGTACC GGTAC^C
2396 GGTACC
MamI 2051 GATTTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2904 GATCCAAATC
NsiI 1533 ATGCAT ATGCA^T
2850 ATGCAT
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AvaI
AvaII AviII BalI BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssHII BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI