################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII AccI AclI AflII AflIII AhdI AscI AseI AsnI Asp700I AspEI AspI BbeI BbsI BbvCI BciVI BclI BfrI BpuAI BsiWI Bsp1407I BspHI BspLU11I BsrGI Bst1107I BstBI BstZ17I CpoI DraIII Eam1105I EcoRV FbaI FseI HindIII HpaI KasI KspI MfeI MluI MunI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PmeI PshAI PshBI Psp1406I RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccIII 3157 TCCGGA T^CCGGA AcsI 2529 GAATTC [GA]^AATT[CT] AgeI 976 ACCGGT A^CCGGT ApoI 2529 GAATTC [GA]^AATT[CT] AvrII 2408 CCTAGG C^CTAGG BamHI 2779 GGATCC G^GATCC BbrPI 1847 CACGTG CAC^GTG BcgI 2056 CGATCCGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 1552 AGATCT A^GATCT BlnI 2408 CCTAGG C^CTAGG BsaAI 1847 CACGTG [CT]AC^GT[GA] BseAI 3157 TCCGGA T^CCGGA BsmBI 3418 CGTCTC CGTCTC(1/5) BsmI 1535 GCATTC GAATGC(1/-1) BspDI 3786 ATCGAT AT^CGAT BspEI 3157 TCCGGA T^CCGGA BstEII 3709 GGTGACC G^GT[ACGT]ACC BstPI 3709 GGTGACC G^GT[ACGT]ACC BstXI 3664 CCAAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 3786 ATCGAT AT^CGAT DraI 3026 TTTAAA TTT^AAA DrdI 3484 GACTTCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1760 GAAGAG CTCTTC(1/4) EcoO65I 3709 GGTGACC G^GT[ACGT]ACC EcoRI 2529 GAATTC G^AATTC Ksp632I 1760 GAAGAG CTCTTC(1/4) MroI 3157 TCCGGA T^CCGGA MslI 3699 CACAAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NheI 1667 GCTAGC G^CTAGC NspI 2852 GCATGT [GA]CATG^[CT] PinAI 976 ACCGGT A^CCGGT PmaCI 1847 CACGTG CAC^GTG PmlI 1847 CACGTG CAC^GTG PvuI 2281 CGATCG CGAT^CG SacI 1546 GAGCTC GAGCT^C ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### Acc65I 1802 GGTACC G^GTACC 2396 GGTACC Alw44I 785 GTGCAC G^TGCAC 1191 GTGCAC Aor51HI 2807 AGCGCT AGC^GCT 3152 AGCGCT ApaI 125 GGGCCC GGGCC^C 1648 GGGCCC ApaLI 785 GTGCAC G^TGCAC 1191 GTGCAC Asp718I 1802 GGTACC G^GTACC 2396 GGTACC BglI 1163 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1684 GCCGGGGAGGC BlpI 2355 GCTGAGC GC^T[ACGT]AGC 3449 GCTCAGC Bpu1102I 2355 GCTGAGC GC^T[ACGT]AGC 3449 GCTCAGC BsaBI 2051 GATTTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2904 GATCCAAATC BsrDI 896 CATTGC GCAATG(2/0) 931 GCAATG CelII 2355 GCTGAGC GC^T[ACGT]AGC 3449 GCTCAGC EagI 1682 CGGCCG C^GGCCG 2046 CGGCCG EclXI 1682 CGGCCG C^GGCCG 2046 CGGCCG Eco47III 2807 AGCGCT AGC^GCT 3152 AGCGCT Eco52I 1682 CGGCCG C^GGCCG 2046 CGGCCG EcoT22I 1533 ATGCAT ATGCA^T 2850 ATGCAT KpnI 1802 GGTACC GGTAC^C 2396 GGTACC MamI 2051 GATTTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2904 GATCCAAATC NsiI 1533 ATGCAT ATGCA^T 2850 ATGCAT ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI AspHI AvaI AvaII AviII BalI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BssSI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI