##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AspI BciVI BglII BlpI Bpu1102I
BsaBI BsaWI BseAI BsiWI BspDI
BspEI BspHI BsrBI BsrDI Bst1107I
BstBI BstZ17I CelII ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoT22I FseI HindIII HpaI
KspI MamI MfeI MroI MunI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SbfI ScaI SexAI SfiI
SfuI SgrAI Sse8387I Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 1073 GGTACC G^GTACC
AflII 3016 CTTAAG C^TTAAG
ApaI 4226 GGGCCC GGGCC^C
Asp718I 1073 GGTACC G^GTACC
AviII 2740 TGCGCA TGC^GCA
BbeI 734 GGCGCC GGCGC^C
BclI 3000 TGATCA T^GATCA
BfrI 3016 CTTAAG C^TTAAG
BsiEI 856 CGGTCG CG[GA][CT]^CG
BspLU11I 3733 ACATGT A^CATGT
BssHII 2587 GCGCGC G^CGCGC
BstXI 2259 CCACCATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoNI 508 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1268 GATATC GAT^ATC
FbaI 3000 TGATCA T^GATCA
FspI 2740 TGCGCA TGC^GCA
HincII 858 GTCGAC GT[CT]^[GA]AC
HindII 858 GTCGAC GT[CT]^[GA]AC
KasI 734 GGCGCC G^GCGCC
KpnI 1073 GGTACC GGTAC^C
MluI 617 ACGCGT A^CGCGT
NarI 734 GGCGCC GG^CGCC
PshAI 662 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1472 GAGCTC GAGCT^C
SalI 858 GTCGAC G^TCGAC
SfoI 734 GGCGCC GGC^GCC
SnaBI 4430 TACGTA TAC^GTA
SpeI 4565 ACTAGT A^CTAGT
SspI 3690 AATATT AAT^ATT
SwaI 4176 ATTTAAAT ATTT^AAAT
TaqI 859 TCGA T^CGA
TthHB8I 859 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 858 GTCGAC GT^[AC][GT]AC
1697 GTCTAC
AcyI 464 GACGCC G[GA]^CG[CT]C
734 GGCGCC
Alw44I 948 GTGCAC G^TGCAC
4546 GTGCAC
AocI 2017 CCTCAGG CC^T[ACGT]AGG
4315 CCTGAGG
ApaLI 948 GTGCAC G^TGCAC
4546 GTGCAC
Asp700I 89 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2454 GAACATATTC
AvrII 2976 CCTAGG C^CTAGG
4230 CCTAGG
BamHI 112 GGATCC G^GATCC
143 GGATCC
BcgI 1530 CGAAGCTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2731 CGAGGCTCATGC
BlnI 2976 CCTAGG C^CTAGG
4230 CCTAGG
BsaHI 464 GACGCC G[GA]^CG[CT]C
734 GGCGCC
BsaI 2398 GAGACC GGTCTC(1/5)
3093 GGTCTC
BssSI 4042 CACGAG CACGAG(-5/-1)
530 CTCGTG
BstEII 1498 GGTAACC G^GT[ACGT]ACC
2033 GGTAACC
BstPI 1498 GGTAACC G^GT[ACGT]ACC
2033 GGTAACC
Bsu36I 2017 CCTCAGG CC^T[ACGT]AGG
4315 CCTGAGG
DraIII 2937 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
3621 CACTCAGTG
DrdI 547 GACTCCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4564 GACTAGTGGGTC
Eco81I 2017 CCTCAGG CC^T[ACGT]AGG
4315 CCTGAGG
EcoO65I 1498 GGTAACC G^GT[ACGT]ACC
2033 GGTAACC
HgaI 464 GACGC GACGC(5/10)
1441 GACGC
Hin1I 464 GACGCC G[GA]^CG[CT]C
734 GGCGCC
NcoI 417 CCATGG C^CATGG
1098 CCATGG
NheI 2069 GCTAGC G^CTAGC
2609 GCTAGC
SphI 1378 GCATGC GCATG^C
2591 GCATGC
StuI 826 AGGCCT AGG^CCT
4380 AGGCCT
XbaI 2049 TCTAGA T^CTAGA
4305 TCTAGA
XcmI 3578 CCACAGTTTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3951 CCAGTGGCCTTCTGG
XmnI 89 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2454 GAACATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrFI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SspBI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII
XmaCI XmaI