################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII AccIII AclI AgeI AhdI Aor51HI AscI AseI AsnI AspEI AspI BciVI BglII BlpI Bpu1102I BsaBI BsaWI BseAI BsiWI BspDI BspEI BspHI BsrBI BsrDI Bst1107I BstBI BstZ17I CelII ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoT22I FseI HindIII HpaI KspI MamI MfeI MroI MunI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SbfI ScaI SexAI SfiI SfuI SgrAI Sse8387I Tth111I XhoI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### Acc65I 1073 GGTACC G^GTACC AflII 3016 CTTAAG C^TTAAG ApaI 4226 GGGCCC GGGCC^C Asp718I 1073 GGTACC G^GTACC AviII 2740 TGCGCA TGC^GCA BbeI 734 GGCGCC GGCGC^C BclI 3000 TGATCA T^GATCA BfrI 3016 CTTAAG C^TTAAG BsiEI 856 CGGTCG CG[GA][CT]^CG BspLU11I 3733 ACATGT A^CATGT BssHII 2587 GCGCGC G^CGCGC BstXI 2259 CCACCATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EcoNI 508 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1268 GATATC GAT^ATC FbaI 3000 TGATCA T^GATCA FspI 2740 TGCGCA TGC^GCA HincII 858 GTCGAC GT[CT]^[GA]AC HindII 858 GTCGAC GT[CT]^[GA]AC KasI 734 GGCGCC G^GCGCC KpnI 1073 GGTACC GGTAC^C MluI 617 ACGCGT A^CGCGT NarI 734 GGCGCC GG^CGCC PshAI 662 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1472 GAGCTC GAGCT^C SalI 858 GTCGAC G^TCGAC SfoI 734 GGCGCC GGC^GCC SnaBI 4430 TACGTA TAC^GTA SpeI 4565 ACTAGT A^CTAGT SspI 3690 AATATT AAT^ATT SwaI 4176 ATTTAAAT ATTT^AAAT TaqI 859 TCGA T^CGA TthHB8I 859 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccI 858 GTCGAC GT^[AC][GT]AC 1697 GTCTAC AcyI 464 GACGCC G[GA]^CG[CT]C 734 GGCGCC Alw44I 948 GTGCAC G^TGCAC 4546 GTGCAC AocI 2017 CCTCAGG CC^T[ACGT]AGG 4315 CCTGAGG ApaLI 948 GTGCAC G^TGCAC 4546 GTGCAC Asp700I 89 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2454 GAACATATTC AvrII 2976 CCTAGG C^CTAGG 4230 CCTAGG BamHI 112 GGATCC G^GATCC 143 GGATCC BcgI 1530 CGAAGCTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2731 CGAGGCTCATGC BlnI 2976 CCTAGG C^CTAGG 4230 CCTAGG BsaHI 464 GACGCC G[GA]^CG[CT]C 734 GGCGCC BsaI 2398 GAGACC GGTCTC(1/5) 3093 GGTCTC BssSI 4042 CACGAG CACGAG(-5/-1) 530 CTCGTG BstEII 1498 GGTAACC G^GT[ACGT]ACC 2033 GGTAACC BstPI 1498 GGTAACC G^GT[ACGT]ACC 2033 GGTAACC Bsu36I 2017 CCTCAGG CC^T[ACGT]AGG 4315 CCTGAGG DraIII 2937 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG 3621 CACTCAGTG DrdI 547 GACTCCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4564 GACTAGTGGGTC Eco81I 2017 CCTCAGG CC^T[ACGT]AGG 4315 CCTGAGG EcoO65I 1498 GGTAACC G^GT[ACGT]ACC 2033 GGTAACC HgaI 464 GACGC GACGC(5/10) 1441 GACGC Hin1I 464 GACGCC G[GA]^CG[CT]C 734 GGCGCC NcoI 417 CCATGG C^CATGG 1098 CCATGG NheI 2069 GCTAGC G^CTAGC 2609 GCTAGC SphI 1378 GCATGC GCATG^C 2591 GCATGC StuI 826 AGGCCT AGG^CCT 4380 AGGCCT XbaI 2049 TCTAGA T^CTAGA 4305 TCTAGA XcmI 3578 CCACAGTTTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3951 CCAGTGGCCTTCTGG XmnI 89 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2454 GAACATATTC ######################################### # 3 OR MORE CUT ENZYMES ( 127 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspBI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII XmaCI XmaI