##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AclI AflIII AgeI AscI
AseI AsnI Asp718I AviII BbeI
BciVI BsaBI BsiWI BspDI BspHI
BspLU11I BssHII Bst1107I BstZ17I ClaI
CpoI DraI DrdI EcoRI EcoRV
EcoT22I FseI FspI HindIII KasI
KpnI KspI MamI MfeI MluI
MunI NarI NdeI NotI NruI
NsiI PacI PinAI PmeI PshBI
Psp1406I RcaI RsrII SacII SalI
SfiI SfoI SgrAI SnaBI SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 1293 GACGTC GACGT^C
AccI 1041 GTCTAC GT^[AC][GT]AC
AccII 3 CGCG CG^CG
AccIII 943 TCCGGA T^CCGGA
AflII 2124 CTTAAG C^TTAAG
AhdI 777 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 935 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 777 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3604 CCTAGG C^CTAGG
BbrPI 951 CACGTG CAC^GTG
BclI 1009 TGATCA T^GATCA
BfrI 2124 CTTAAG C^TTAAG
BlnI 3604 CCTAGG C^CTAGG
BsaI 2802 GAGACC GGTCTC(1/5)
BseAI 943 TCCGGA T^CCGGA
BsmBI 876 GAGACG CGTCTC(1/5)
BsmI 2493 GAATGC GAATGC(1/-1)
BspEI 943 TCCGGA T^CCGGA
BsrDI 2144 CATTGC GCAATG(2/0)
BssSI 576 CTCGTG CACGAG(-5/-1)
BstAPI 2423 GCAGTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 644 TTCGAA TT^CGAA
BstEII 371 GGTCACC G^GT[ACGT]ACC
BstPI 371 GGTCACC G^GT[ACGT]ACC
BstUI 3 CGCG CG^CG
EagI 15 CGGCCG C^GGCCG
Eam1105I 777 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 15 CGGCCG C^GGCCG
Eco52I 15 CGGCCG C^GGCCG
EcoO65I 371 GGTCACC G^GT[ACGT]ACC
FbaI 1009 TGATCA T^GATCA
HincII 1965 GTTAAC GT[CT]^[GA]AC
HindII 1965 GTTAAC GT[CT]^[GA]AC
HpaI 1965 GTTAAC GTT^AAC
MroI 943 TCCGGA T^CCGGA
MvnI 3 CGCG CG^CG
NaeI 3908 GCCGGC GCC^GGC
NgoMIV 3908 GCCGGC G^CCGGC
NheI 3773 GCTAGC G^CTAGC
NspV 644 TTCGAA TT^CGAA
PmaCI 951 CACGTG CAC^GTG
PmlI 951 CACGTG CAC^GTG
PshAI 4095 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 870 CGATCG CGAT^CG
SbfI 2039 CCTGCAGG CCTGCA^GG
SexAI 1525 ACCTGGT A^CC[AT]GGT
SfuI 644 TTCGAA TT^CGAA
SmaI 38 CCCGGG CCC^GGG
SpeI 2820 ACTAGT A^CTAGT
SphI 3427 GCATGC GCATG^C
Sse8387I 2039 CCTGCAGG CCTGCA^GG
XcmI 640 CCAGTTCGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 38 CCCGGG C^CCGGG
XmaI 38 CCCGGG C^CCGGG
XmnI 935 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 458 GACGCC G[GA]^CG[CT]C
1293 GACGTC
Alw44I 1050 GTGCAC G^TGCAC
1893 GTGCAC
Aor51HI 1357 AGCGCT AGC^GCT
2357 AGCGCT
ApaLI 1050 GTGCAC G^TGCAC
1893 GTGCAC
AspI 836 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3437 GACCCTGTC
BamHI 247 GGATCC G^GATCC
3333 GGATCC
BanI 1926 GGCACC G^G[CT][GA]CC
3740 GGCACC
BbvCI 142 CCTCAGC CCTCAGC(-5/-2)
2267 CCTCAGC
BcgI 1643 CGAGGCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2206 GCAACAAAATCG
BglII 1150 AGATCT A^GATCT
2923 AGATCT
BlpI 1496 GCTCAGC GC^T[ACGT]AGC
2946 GCTGAGC
Bpu1102I 1496 GCTCAGC GC^T[ACGT]AGC
2946 GCTGAGC
BsaAI 516 TACGTG [CT]AC^GT[GA]
951 CACGTG
BsaHI 458 GACGCC G[GA]^CG[CT]C
1293 GACGTC
BsiEI 15 CGGCCG CG[GA][CT]^CG
870 CGATCG
Bsp1407I 982 TGTACA T^GTACA
1204 TGTACA
BsrBI 63 GAGCGG CCGCTC(-3/-3)
2592 GAGCGG
BsrFI 2485 ACCGGC [GA]^CCGG[CT]
3908 GCCGGC
BsrGI 982 TGTACA T^GTACA
1204 TGTACA
BstXI 3685 CCATACCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3926 CCAGGGAATTGG
CelII 1496 GCTCAGC GC^T[ACGT]AGC
2946 GCTGAGC
Cfr10I 2485 ACCGGC [GA]^CCGG[CT]
3908 GCCGGC
DraIII 657 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
1208 CACACTGTG
Eco47III 1357 AGCGCT AGC^GCT
2357 AGCGCT
Hin1I 458 GACGCC G[GA]^CG[CT]C
1293 GACGTC
PflFI 836 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3437 GACCCTGTC
PpuMI 1540 AGGTCCT [GA]G^G[AT]CC[CT]
4003 AGGTCCC
ScaI 562 AGTACT AGT^ACT
1243 AGTACT
SspBI 982 TGTACA T^GTACA
1204 TGTACA
Tth111I 836 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3437 GACCCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BalI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PaeR7I
PflMI PleI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoI
XhoII