##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AscI Asp718I AvrII BamHI
BbeI BbrPI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BssHII BstBI
BstXI CelII ClaI CpoI DrdI
EagI EclXI Eco52I FbaI FseI
Hin1I KasI KpnI KspI MfeI
MluI MroI MunI NarI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmlI PvuI RcaI RsrII
SacII SalI SapI ScaI SfiI
SfoI SfuI SgrAI SnaBI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AclI 2550 AACGTT AA^CGTT
AhdI 2052 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4529 GTGCAC G^TGCAC
ApaLI 4529 GTGCAC G^TGCAC
AseI 2280 ATTAAT AT^TAAT
AsnI 2280 ATTAAT AT^TAAT
Asp700I 2547 GAAAACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2052 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1070 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BsaBI 425 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2255 ACCGGA [AT]^CCGG[AT]
BsmI 183 GCATTC GAATGC(1/-1)
BsrFI 901 GCCGGC [GA]^CCGG[CT]
BssSI 1167 CACGAG CACGAG(-5/-1)
Bst1107I 2802 GTATAC GTA^TAC
BstZ17I 2802 GTATAC GTA^TAC
Cfr10I 901 GCCGGC [GA]^CCGG[CT]
Eam1105I 2052 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1457 GAATTC G^AATTC
EcoT22I 2515 ATGCAT ATGCA^T
HindIII 2574 AAGCTT A^AGCTT
HpaI 2989 GTTAAC GTT^AAC
MamI 425 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 901 GCCGGC GCC^GGC
NdeI 2504 CATATG CA^TATG
NgoMIV 901 GCCGGC G^CCGGC
NsiI 2515 ATGCAT ATGCA^T
PflFI 1070 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3512 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 2788 GTTTAAAC GTTT^AAAC
PshBI 2280 ATTAAT AT^TAAT
Psp1406I 2550 AACGTT AA^CGTT
SbfI 34 CCTGCAGG CCTGCA^GG
SmaI 535 CCCGGG CCC^GGG
SpeI 2581 ACTAGT A^CTAGT
Sse8387I 34 CCTGCAGG CCTGCA^GG
SspI 4381 AATATT AAT^ATT
StuI 2296 AGGCCT AGG^CCT
Tth111I 1070 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3512 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1269 TCTAGA T^CTAGA
XmaCI 535 CCCGGG C^CCGGG
XmaI 535 CCCGGG C^CCGGG
XmnI 2547 GAAAACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 2802 GTATAC GT^[AC][GT]AC
3951 GTCTAC
AviII 821 TGCGCA TGC^GCA
2402 TGCGCA
BcgI 2474 CGATACTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
138 GCAGCTCTGTCG
BglI 1243 GCCAGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1317 GCCTCACGGGC
BsaI 1218 GAGACC GGTCTC(1/5)
3688 GGTCTC
Bsp1407I 2679 TGTACA T^GTACA
3409 TGTACA
BsrGI 2679 TGTACA T^GTACA
3409 TGTACA
BstEII 343 GGTGACC G^GT[ACGT]ACC
4588 GGTGACC
BstPI 343 GGTGACC G^GT[ACGT]ACC
4588 GGTGACC
BstYI 1266 GGATCT [GA]^GATC[CT]
1371 AGATCC
DraI 2789 TTTAAA TTT^AAA
4365 TTTAAA
EcoO65I 343 GGTGACC G^GT[ACGT]ACC
4588 GGTGACC
EcoRV 429 GATATC GAT^ATC
932 GATATC
FspI 821 TGCGCA TGC^GCA
2402 TGCGCA
MflI 1266 GGATCT [GA]^GATC[CT]
1371 AGATCC
NcoI 1350 CCATGG C^CATGG
1500 CCATGG
NheI 359 GCTAGC G^CTAGC
2997 GCTAGC
PshAI 4557 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4662 GACACCTGTC
SacI 1551 GAGCTC GAGCT^C
4476 GAGCTC
SexAI 195 ACCTGGT A^CC[AT]GGT
862 ACCTGGT
SphI 363 GCATGC GCATG^C
2635 GCATGC
SspBI 2679 TGTACA T^GTACA
3409 TGTACA
XhoII 1266 GGATCT [GA]^GATC[CT]
1371 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI Aor51HI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrBI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco47III Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI