##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AscI Asp718I AspI AviII BamHI
BsaBI BseAI BsiEI BsiWI BsmI
BspDI BspEI BssHII BssSI BstBI
BstUI ClaI CpoI EagI EclXI
Eco52I EcoRV FseI FspI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NdeI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI Psp1406I PvuI RsrII SacI
SacII SbfI ScaI SexAI SfiI
SfuI SmaI SnaBI Sse8387I SwaI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AhdI 1567 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1327 GTGCAC G^TGCAC
Aor51HI 3435 AGCGCT AGC^GCT
ApaLI 1327 GTGCAC G^TGCAC
AseI 3868 ATTAAT AT^TAAT
AsnI 3868 ATTAAT AT^TAAT
AspEI 1567 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3428 CCTAGG C^CTAGG
BbrPI 1265 CACGTG CAC^GTG
BbsI 2940 GAAGAC GAAGAC(2/6)
BclI 867 TGATCA T^GATCA
BglII 3752 AGATCT A^GATCT
BlnI 3428 CCTAGG C^CTAGG
BlpI 2317 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2317 GCTCAGC GC^T[ACGT]AGC
BpuAI 2940 GAAGAC GAAGAC(2/6)
BsaAI 1265 CACGTG [CT]AC^GT[GA]
BsaI 3125 GAGACC GGTCTC(1/5)
BsaWI 2596 TCCGGT [AT]^CCGG[AT]
BsmBI 2921 CGTCTC CGTCTC(1/5)
BspHI 3442 TCATGA T^CATGA
Bst1107I 3183 GTATAC GTA^TAC
BstXI 1959 CCAGCAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 3752 AGATCT [GA]^GATC[CT]
BstZ17I 3183 GTATAC GTA^TAC
CelII 2317 GCTCAGC GC^T[ACGT]AGC
Eam1105I 1567 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3435 AGCGCT AGC^GCT
EcoNI 1005 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1461 GAATTC G^AATTC
FauI 560 CCCGC CCCGC(4/6)
FbaI 867 TGATCA T^GATCA
HindIII 112 AAGCTT A^AGCTT
HpaI 3675 GTTAAC GTT^AAC
MflI 3752 AGATCT [GA]^GATC[CT]
NheI 2584 GCTAGC G^CTAGC
PflMI 1117 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1265 CACGTG CAC^GTG
PmlI 1265 CACGTG CAC^GTG
PpuMI 1393 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 3971 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3868 ATTAAT AT^TAAT
RcaI 3442 TCATGA T^CATGA
SalI 422 GTCGAC G^TCGAC
SgrAI 436 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 2119 ACTAGT A^CTAGT
StuI 242 AGGCCT AGG^CCT
Van91I 1117 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2785 CCATAACAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 3752 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 459 GACGTC GACGT^C
3352 GACGTC
AccI 422 GTCGAC GT^[AC][GT]AC
3183 GTATAC
ApaI 1942 GGGCCC GGGCC^C
2066 GGGCCC
Asp700I 4069 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4232 GAAAAATTTC
BbeI 15 GGCGCC GGCGC^C
434 GGCGCC
BcgI 465 CGAGGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2354 CGAAGTTGATGC
Bsp1407I 1242 TGTACA T^GTACA
1514 TGTACA
BspLU11I 704 ACATGT A^CATGT
4192 ACATGT
BsrBI 295 CCGCTC CCGCTC(-3/-3)
2315 CCGCTC
BsrDI 4227 GCAATG GCAATG(2/0)
4278 GCAATG
BsrGI 1242 TGTACA T^GTACA
1514 TGTACA
BstEII 1112 GGTTACC G^GT[ACGT]ACC
2196 GGTAACC
BstPI 1112 GGTTACC G^GT[ACGT]ACC
2196 GGTAACC
DraIII 2685 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2885 CACAAGGTG
DrdI 1253 GACCACCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3895 GACATCTGTGTC
EcoO65I 1112 GGTTACC G^GT[ACGT]ACC
2196 GGTAACC
KasI 15 GGCGCC G^GCGCC
434 GGCGCC
MslI 324 CACGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1330 CACCCAGATG
NaeI 437 GCCGGC GCC^GGC
1347 GCCGGC
NarI 15 GGCGCC GG^CGCC
434 GGCGCC
NgoMIV 437 GCCGGC G^CCGGC
1347 GCCGGC
SfoI 15 GGCGCC GGC^GCC
434 GGCGCC
SphI 3307 GCATGC GCATG^C
3636 GCATGC
SspBI 1242 TGTACA T^GTACA
1514 TGTACA
XmnI 4069 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4232 GAAAAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflII
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BciVI
BcnI BfaI BfrI BglI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PleI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I