################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI Alw44I AocI ApaLI AscI AseI AsnI Asp718I AviII BbrPI BcgI BciVI BfrI BglI BsaAI BseAI BsiWI Bsp1407I BspEI BspHI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I CpoI DraIII DrdI EagI EclXI Eco52I Eco81I EcoRI EcoRV FseI FspI HindIII KpnI MluI MroI NaeI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I RcaI RsrII SalI SapI ScaI SfiI SfuI SgrAI SnaBI SspBI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 2213 GTAGAC GT^[AC][GT]AC AcyI 197 GGCGCC G[GA]^CG[CT]C AflIII 2371 ACATGT A^C[GA][CT]GT AhdI 718 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1698 AGCGCT AGC^GCT Asp700I 1906 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 718 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1477 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2105 GGATCC G^GATCC BbeI 197 GGCGCC GGCGC^C BbsI 2403 GAAGAC GAAGAC(2/6) BglII 1706 AGATCT A^GATCT BpuAI 2403 GAAGAC GAAGAC(2/6) BsaHI 197 GGCGCC G[GA]^CG[CT]C BsiEI 627 CGATCG CG[GA][CT]^CG BsmI 1960 GCATTC GAATGC(1/-1) BspDI 748 ATCGAT AT^CGAT BspLU11I 2371 ACATGT A^CATGT BsrDI 3702 CATTGC GCAATG(2/0) BstAPI 3697 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC ClaI 748 ATCGAT AT^CGAT DraI 2332 TTTAAA TTT^AAA Eam1105I 718 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1698 AGCGCT AGC^GCT EcoT22I 3347 ATGCAT ATGCA^T Hin1I 197 GGCGCC G[GA]^CG[CT]C HpaI 2346 GTTAAC GTT^AAC KasI 197 GGCGCC G^GCGCC KspI 4237 CCGCGG CCGC^GG MfeI 2179 CAATTG C^AATTG MunI 2179 CAATTG C^AATTG NarI 197 GGCGCC GG^CGCC NcoI 4270 CCATGG C^CATGG NheI 2140 GCTAGC G^CTAGC NsiI 3347 ATGCAT ATGCA^T PflFI 1477 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 656 CCAACCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 3369 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 627 CGATCG CGAT^CG SacI 4506 GAGCTC GAGCT^C SacII 4237 CCGCGG CCGC^GG SbfI 4121 CCTGCAGG CCTGCA^GG SexAI 4485 ACCTGGT A^CC[AT]GGT SfoI 197 GGCGCC GGC^GCC SmaI 817 CCCGGG CCC^GGG SpeI 1847 ACTAGT A^CTAGT SphI 2648 GCATGC GCATG^C Sse8387I 4121 CCTGCAGG CCTGCA^GG Tth111I 1477 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 656 CCAACCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2053 TCTAGA T^CTAGA XmaCI 817 CCCGGG C^CCGGG XmaI 817 CCCGGG C^CCGGG XmnI 1906 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccII 3435 CGCG CG^CG 4238 CGCG ApaI 464 GGGCCC GGGCC^C 3429 GGGCCC AvrII 4436 CCTAGG C^CTAGG 4639 CCTAGG BanI 197 GGCGCC G^G[CT][GA]CC 3993 GGCACC BbvCI 201 CCTCAGC CCTCAGC(-5/-2) 781 GCTGAGG BlnI 4436 CCTAGG C^CTAGG 4639 CCTAGG BsaWI 602 TCCGGT [AT]^CCGG[AT] 2271 ACCGGA BsgI 2725 CTGCAC GTGCAG(16/14) 3359 CTGCAC BsmBI 1557 CGTCTC CGTCTC(1/5) 4039 GAGACG BsrFI 1526 ACCGGC [GA]^CCGG[CT] 1607 ACCGGC BssSI 2919 CTCGTG CACGAG(-5/-1) 3632 CTCGTG BstEII 1807 GGTTACC G^GT[ACGT]ACC 3827 GGTCACC BstPI 1807 GGTTACC G^GT[ACGT]ACC 3827 GGTCACC BstUI 3435 CGCG CG^CG 4238 CGCG BstXI 987 CCACAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3076 CCATCCAGATGG Cfr10I 1526 ACCGGC [GA]^CCGG[CT] 1607 ACCGGC EarI 2494 CTCTTC CTCTTC(1/4) 2817 GAAGAG EcoO65I 1807 GGTTACC G^GT[ACGT]ACC 3827 GGTCACC Ksp632I 2494 CTCTTC CTCTTC(1/4) 2817 GAAGAG MvnI 3435 CGCG CG^CG 4238 CGCG PpuMI 1948 GGGTCCT [GA]G^G[AT]CC[CT] 3200 GGGACCC XcmI 2756 CCAAAGCATCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4270 CCATGGGCAGAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanII BbvI BclI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaBI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII