##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I AocI ApaLI AscI
AseI AsnI Asp718I AviII BbrPI
BcgI BciVI BfrI BglI BsaAI
BseAI BsiWI Bsp1407I BspEI BspHI
BsrGI BssHII Bst1107I BstBI BstZ17I
Bsu36I CpoI DraIII DrdI EagI
EclXI Eco52I Eco81I EcoRI EcoRV
FseI FspI HindIII KpnI MluI
MroI NaeI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
RcaI RsrII SalI SapI ScaI
SfiI SfuI SgrAI SnaBI SspBI
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 2213 GTAGAC GT^[AC][GT]AC
AcyI 197 GGCGCC G[GA]^CG[CT]C
AflIII 2371 ACATGT A^C[GA][CT]GT
AhdI 718 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1698 AGCGCT AGC^GCT
Asp700I 1906 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 718 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1477 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2105 GGATCC G^GATCC
BbeI 197 GGCGCC GGCGC^C
BbsI 2403 GAAGAC GAAGAC(2/6)
BglII 1706 AGATCT A^GATCT
BpuAI 2403 GAAGAC GAAGAC(2/6)
BsaHI 197 GGCGCC G[GA]^CG[CT]C
BsiEI 627 CGATCG CG[GA][CT]^CG
BsmI 1960 GCATTC GAATGC(1/-1)
BspDI 748 ATCGAT AT^CGAT
BspLU11I 2371 ACATGT A^CATGT
BsrDI 3702 CATTGC GCAATG(2/0)
BstAPI 3697 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 748 ATCGAT AT^CGAT
DraI 2332 TTTAAA TTT^AAA
Eam1105I 718 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1698 AGCGCT AGC^GCT
EcoT22I 3347 ATGCAT ATGCA^T
Hin1I 197 GGCGCC G[GA]^CG[CT]C
HpaI 2346 GTTAAC GTT^AAC
KasI 197 GGCGCC G^GCGCC
KspI 4237 CCGCGG CCGC^GG
MfeI 2179 CAATTG C^AATTG
MunI 2179 CAATTG C^AATTG
NarI 197 GGCGCC GG^CGCC
NcoI 4270 CCATGG C^CATGG
NheI 2140 GCTAGC G^CTAGC
NsiI 3347 ATGCAT ATGCA^T
PflFI 1477 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 656 CCAACCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3369 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 627 CGATCG CGAT^CG
SacI 4506 GAGCTC GAGCT^C
SacII 4237 CCGCGG CCGC^GG
SbfI 4121 CCTGCAGG CCTGCA^GG
SexAI 4485 ACCTGGT A^CC[AT]GGT
SfoI 197 GGCGCC GGC^GCC
SmaI 817 CCCGGG CCC^GGG
SpeI 1847 ACTAGT A^CTAGT
SphI 2648 GCATGC GCATG^C
Sse8387I 4121 CCTGCAGG CCTGCA^GG
Tth111I 1477 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 656 CCAACCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2053 TCTAGA T^CTAGA
XmaCI 817 CCCGGG C^CCGGG
XmaI 817 CCCGGG C^CCGGG
XmnI 1906 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 3435 CGCG CG^CG
4238 CGCG
ApaI 464 GGGCCC GGGCC^C
3429 GGGCCC
AvrII 4436 CCTAGG C^CTAGG
4639 CCTAGG
BanI 197 GGCGCC G^G[CT][GA]CC
3993 GGCACC
BbvCI 201 CCTCAGC CCTCAGC(-5/-2)
781 GCTGAGG
BlnI 4436 CCTAGG C^CTAGG
4639 CCTAGG
BsaWI 602 TCCGGT [AT]^CCGG[AT]
2271 ACCGGA
BsgI 2725 CTGCAC GTGCAG(16/14)
3359 CTGCAC
BsmBI 1557 CGTCTC CGTCTC(1/5)
4039 GAGACG
BsrFI 1526 ACCGGC [GA]^CCGG[CT]
1607 ACCGGC
BssSI 2919 CTCGTG CACGAG(-5/-1)
3632 CTCGTG
BstEII 1807 GGTTACC G^GT[ACGT]ACC
3827 GGTCACC
BstPI 1807 GGTTACC G^GT[ACGT]ACC
3827 GGTCACC
BstUI 3435 CGCG CG^CG
4238 CGCG
BstXI 987 CCACAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3076 CCATCCAGATGG
Cfr10I 1526 ACCGGC [GA]^CCGG[CT]
1607 ACCGGC
EarI 2494 CTCTTC CTCTTC(1/4)
2817 GAAGAG
EcoO65I 1807 GGTTACC G^GT[ACGT]ACC
3827 GGTCACC
Ksp632I 2494 CTCTTC CTCTTC(1/4)
2817 GAAGAG
MvnI 3435 CGCG CG^CG
4238 CGCG
PpuMI 1948 GGGTCCT [GA]G^G[AT]CC[CT]
3200 GGGACCC
XcmI 2756 CCAAAGCATCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4270 CCATGGGCAGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BalI BanII BbvI BclI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaBI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII