##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AspI AvrII BglII
BlnI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrFI BssHII BssSI BstAPI BstEII
BstPI BstUI Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoT22I FseI HpaI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NdeI
NgoMIV NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I Van91I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Alw44I 3517 GTGCAC G^TGCAC
ApaLI 3517 GTGCAC G^TGCAC
AviII 1029 TGCGCA TGC^GCA
BalI 2436 TGGCCA TGG^CCA
BamHI 640 GGATCC G^GATCC
BcgI 2314 GCAGCAGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsmI 2099 GCATTC GAATGC(1/-1)
Bsp1407I 1893 TGTACA T^GTACA
BspHI 1077 TCATGA T^CATGA
BsrBI 3078 CCGCTC CCGCTC(-3/-3)
BsrGI 1893 TGTACA T^GTACA
Bst1107I 991 GTATAC GTA^TAC
BstBI 786 TTCGAA TT^CGAA
BstZ17I 991 GTATAC GTA^TAC
DraIII 3052 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 860 GATATC GAT^ATC
FspI 1029 TGCGCA TGC^GCA
HincII 1834 GTCAAC GT[CT]^[GA]AC
HindII 1834 GTCAAC GT[CT]^[GA]AC
HindIII 1004 AAGCTT A^AGCTT
MluNI 2436 TGGCCA TGG^CCA
MscI 2436 TGGCCA TGG^CCA
NheI 341 GCTAGC G^CTAGC
NspV 786 TTCGAA TT^CGAA
RcaI 1077 TCATGA T^CATGA
SapI 2084 GAAGAGC GCTCTTC(1/4)
SfuI 786 TTCGAA TT^CGAA
SmaI 292 CCCGGG CCC^GGG
SspBI 1893 TGTACA T^GTACA
StuI 3247 AGGCCT AGG^CCT
XcmI 3424 CCAGATGCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 292 CCCGGG C^CCGGG
XmaI 292 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 991 GTATAC GT^[AC][GT]AC
1180 GTCTAC
AcyI 957 GGCGCC G[GA]^CG[CT]C
2972 GGCGCC
AflII 1418 CTTAAG C^TTAAG
1628 CTTAAG
Aor51HI 531 AGCGCT AGC^GCT
1611 AGCGCT
AvaI 292 CCCGGG C^[CT]CG[GA]G
3259 CCCGAG
BbeI 957 GGCGCC GGCGC^C
2972 GGCGCC
BbrPI 374 CACGTG CAC^GTG
2999 CACGTG
BbsI 2213 GAAGAC GAAGAC(2/6)
2351 GAAGAC
BbvCI 74 CCTCAGC CCTCAGC(-5/-2)
2916 GCTGAGG
BciVI 217 GTATCC GTATCC(6/5)
274 GTATCC
BfrI 1418 CTTAAG C^TTAAG
1628 CTTAAG
BglI 2438 GCCAGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3187 GCCGAGTAGGC
BpuAI 2213 GAAGAC GAAGAC(2/6)
2351 GAAGAC
BsaHI 957 GGCGCC G[GA]^CG[CT]C
2972 GGCGCC
BsoBI 292 CCCGGG C^[CT]CG[GA]G
3259 CCCGAG
BspMI 450 ACCTGC ACCTGC(4/8)
1813 GCAGGT
BsrDI 940 CATTGC GCAATG(2/0)
2655 GCAATG
EaeI 2436 TGGCCA [CT]^GGCC[GA]
3059 TGGCCG
Eco47III 531 AGCGCT AGC^GCT
1611 AGCGCT
EcoRI 554 GAATTC G^AATTC
4088 GAATTC
FauI 15 CCCGC CCCGC(4/6)
2635 GCGGG
HgaI 2320 GCGTC GACGC(5/10)
3618 GCGTC
Hin1I 957 GGCGCC G[GA]^CG[CT]C
2972 GGCGCC
KasI 957 GGCGCC G^GCGCC
2972 GGCGCC
NarI 957 GGCGCC GG^CGCC
2972 GGCGCC
PmaCI 374 CACGTG CAC^GTG
2999 CACGTG
PmlI 374 CACGTG CAC^GTG
2999 CACGTG
SacI 398 GAGCTC GAGCT^C
686 GAGCTC
SfoI 957 GGCGCC GGC^GCC
2972 GGCGCC
TaqI 787 TCGA T^CGA
2601 TCGA
TthHB8I 787 TCGA T^CGA
2601 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApaI ApoI
Asp700I AspHI AvaII BanI BanII
BbvI BclI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspLU11I BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PshAI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII XmnI