##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
AscI Asp718I AspEI AvaI AviII
AvrII BanI BbeI BbrPI BciVI
BcnI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
KpnI KspI MamI MluI MroI
MspI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AciI 243 GCGG CCGC(-3/-1)
AflIII 2069 ACGTGT A^C[GA][CT]GT
Alw44I 3333 GTGCAC G^TGCAC
AocI 1921 CCTTAGG CC^T[ACGT]AGG
ApaI 2037 GGGCCC GGGCC^C
ApaLI 3333 GTGCAC G^TGCAC
AseI 3690 ATTAAT AT^TAAT
AsnI 3690 ATTAAT AT^TAAT
Asp700I 2112 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 590 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 838 GGATCC G^GATCC
BglI 4160 GCCTGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
Bsp1407I 1129 TGTACA T^GTACA
BsrGI 1129 TGTACA T^GTACA
BstAPI 1564 GCAGGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2302 CCATTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1921 CCTTAGG CC^T[ACGT]AGG
DraIII 1950 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1921 CCTTAGG CC^T[ACGT]AGG
EcoRI 559 GAATTC G^AATTC
FauI 243 GCGGG CCCGC(4/6)
HgaI 1774 GCGTC GACGC(5/10)
HincII 3870 GTTGAC GT[CT]^[GA]AC
HindII 3870 GTTGAC GT[CT]^[GA]AC
MfeI 833 CAATTG C^AATTG
MunI 833 CAATTG C^AATTG
NdeI 2149 CATATG CA^TATG
PflFI 590 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3548 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 3690 ATTAAT AT^TAAT
PstI 1451 CTGCAG CTGCA^G
ScaI 2609 AGTACT AGT^ACT
SphI 312 GCATGC GCATG^C
SspBI 1129 TGTACA T^GTACA
SwaI 3107 ATTTAAAT ATTT^AAAT
Tth111I 590 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2971 TCTAGA T^CTAGA
XmnI 2112 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccI 446 GTCTAC GT^[AC][GT]AC
4190 GTCTAC
BalI 779 TGGCCA TGG^CCA
2163 TGGCCA
BanII 268 GGGCTC G[GA]GC[CT]^C
2037 GGGCCC
BbvCI 3820 CCTCAGC CCTCAGC(-5/-2)
1763 GCTGAGG
BcgI 435 GCAGCACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
713 GCATCCAATTCG
BclI 519 TGATCA T^GATCA
2153 TGATCA
BsaI 3540 GAGACC GGTCTC(1/5)
3836 GGTCTC
BseRI 3605 CTCCTC GAGGAG(10/8)
3652 CTCCTC
FbaI 519 TGATCA T^GATCA
2153 TGATCA
MluNI 779 TGGCCA TGG^CCA
2163 TGGCCA
MscI 779 TGGCCA TGG^CCA
2163 TGGCCA
NspI 312 GCATGC [GA]CATG^[CT]
1132 ACATGC
SfcI 1066 CTACAG C^T[GA][CT]AG
1451 CTGCAG
SspI 3143 AATATT AAT^ATT
3942 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AspHI AvaII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoRV
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NcoI NdeII NlaIII NlaIV
NsiI PflMI PleI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII