################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## AatII AflII AgeI Aor51HI AscI AviII BclI BfrI BsaBI BsiWI BsmBI BspDI BspHI BspLU11I BssHII Bst1107I BstBI BstZ17I ClaI CpoI Eco47III FbaI FspI HincII HindII HindIII HpaI KspI MamI MluI NdeI NotI NruI NspV PacI PaeR7I PinAI PshAI PvuI RcaI RsrII SacII SalI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccIII 1061 TCCGGA T^CCGGA AclI 2855 AACGTT AA^CGTT AcyI 3767 GGCGCC G[GA]^CG[CT]C AflIII 2581 ACACGT A^C[GA][CT]GT AhdI 2391 GACCGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3394 GTGCAC G^TGCAC ApaI 1171 GGGCCC GGGCC^C ApaLI 3394 GTGCAC G^TGCAC AseI 3144 ATTAAT AT^TAAT AsnI 3144 ATTAAT AT^TAAT AspEI 2391 GACCGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 2625 CCCGGG C^[CT]CG[GA]G BamHI 2088 GGATCC G^GATCC BbeI 3767 GGCGCC GGCGC^C BcgI 3320 CGACATCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3195 GTATCC GTATCC(6/5) BglII 3032 AGATCT A^GATCT BsaHI 3767 GGCGCC G[GA]^CG[CT]C BseAI 1061 TCCGGA T^CCGGA BsiEI 268 CGGCCG CG[GA][CT]^CG BsoBI 2625 CCCGGG C^[CT]CG[GA]G BspEI 1061 TCCGGA T^CCGGA BsrDI 2932 CATTGC GCAATG(2/0) BssSI 11 CTCGTG CACGAG(-5/-1) BstEII 1107 GGTAACC G^GT[ACGT]ACC BstPI 1107 GGTAACC G^GT[ACGT]ACC DraI 1228 TTTAAA TTT^AAA EagI 268 CGGCCG C^GGCCG Eam1105I 2391 GACCGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 268 CGGCCG C^GGCCG Eco52I 268 CGGCCG C^GGCCG EcoO65I 1107 GGTAACC G^GT[ACGT]ACC EcoRI 3931 GAATTC G^AATTC EcoRV 1554 GATATC GAT^ATC EcoT22I 2067 ATGCAT ATGCA^T FseI 269 GGCCGGCC GGCCGG^CC HgaI 3120 GACGC GACGC(5/10) Hin1I 3767 GGCGCC G[GA]^CG[CT]C KasI 3767 GGCGCC G^GCGCC MfeI 1457 CAATTG C^AATTG MroI 1061 TCCGGA T^CCGGA MunI 1457 CAATTG C^AATTG NarI 3767 GGCGCC GG^CGCC NsiI 2067 ATGCAT ATGCA^T NspI 3489 ACATGC [GA]CATG^[CT] PflMI 3824 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmeI 1227 GTTTAAAC GTTT^AAAC PshBI 3144 ATTAAT AT^TAAT Psp1406I 2855 AACGTT AA^CGTT SbfI 3917 CCTGCAGG CCTGCA^GG SfoI 3767 GGCGCC GGC^GCC SmaI 2625 CCCGGG CCC^GGG Sse8387I 3917 CCTGCAGG CCTGCA^GG Van91I 3824 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3214 TCTAGA T^CTAGA XmaCI 2625 CCCGGG C^CCGGG XmaI 2625 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 1718 GGTACC G^GTACC 3258 GGTACC AccI 3002 GTAGAC GT^[AC][GT]AC 3362 GTCTAC AccII 497 CGCG CG^CG 2698 CGCG AcsI 1965 GAATTT [GA]^AATT[CT] 3931 GAATTC AlwI 2089 GATCC GGATC(4/5) 2088 GGATC AocI 1544 CCTGAGG CC^T[ACGT]AGG 2604 CCTCAGG ApoI 1965 GAATTT [GA]^AATT[CT] 3931 GAATTC Asp718I 1718 GGTACC G^GTACC 3258 GGTACC AvrII 2207 CCTAGG C^CTAGG 3167 CCTAGG BbrPI 594 CACGTG CAC^GTG 2582 CACGTG BbsI 1603 GTCTTC GAAGAC(2/6) 4085 GTCTTC BbvCI 969 CCTCAGC CCTCAGC(-5/-2) 2099 CCTCAGC BglI 290 GCCTGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 294 GCCGGGCCGGC BlnI 2207 CCTAGG C^CTAGG 3167 CCTAGG BpuAI 1603 GTCTTC GAAGAC(2/6) 4085 GTCTTC BsaAI 594 CACGTG [CT]AC^GT[GA] 2582 CACGTG BsmI 125 GAATGC GAATGC(1/-1) 1648 GCATTC BsrBI 1145 CCGCTC CCGCTC(-3/-3) 3924 GAGCGG BstAPI 3487 GCACATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3906 GCAGGACTTGC BstUI 497 CGCG CG^CG 2698 CGCG BstYI 2088 GGATCC [GA]^GATC[CT] 3032 AGATCT Bsu36I 1544 CCTGAGG CC^T[ACGT]AGG 2604 CCTCAGG DrdI 4076 GACTCCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4369 GACTGCAGGGTC Eco81I 1544 CCTGAGG CC^T[ACGT]AGG 2604 CCTCAGG EcoNI 957 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3403 CCTGGGCCAGG KpnI 1718 GGTACC GGTAC^C 3258 GGTACC MflI 2088 GGATCC [GA]^GATC[CT] 3032 AGATCT MvnI 497 CGCG CG^CG 2698 CGCG NcoI 1112 CCATGG C^CATGG 3351 CCATGG PmaCI 594 CACGTG CAC^GTG 2582 CACGTG PmlI 594 CACGTG CAC^GTG 2582 CACGTG SexAI 1501 ACCAGGT A^CC[AT]GGT 1721 ACCAGGT XhoII 2088 GGATCC [GA]^GATC[CT] 3032 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AciI AfaI AluI AlwNI Asp700I AspHI AspI AvaII BalI BanI BanII BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstXI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NaeI NciI NdeII NgoMIV NheI NlaIII NlaIV PflFI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XmnI