##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AflII AgeI Aor51HI AscI
AviII BclI BfrI BsaBI BsiWI
BsmBI BspDI BspHI BspLU11I BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
Eco47III FbaI FspI HincII HindII
HindIII HpaI KspI MamI MluI
NdeI NotI NruI NspV PacI
PaeR7I PinAI PshAI PvuI RcaI
RsrII SacII SalI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccIII 1061 TCCGGA T^CCGGA
AclI 2855 AACGTT AA^CGTT
AcyI 3767 GGCGCC G[GA]^CG[CT]C
AflIII 2581 ACACGT A^C[GA][CT]GT
AhdI 2391 GACCGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3394 GTGCAC G^TGCAC
ApaI 1171 GGGCCC GGGCC^C
ApaLI 3394 GTGCAC G^TGCAC
AseI 3144 ATTAAT AT^TAAT
AsnI 3144 ATTAAT AT^TAAT
AspEI 2391 GACCGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2625 CCCGGG C^[CT]CG[GA]G
BamHI 2088 GGATCC G^GATCC
BbeI 3767 GGCGCC GGCGC^C
BcgI 3320 CGACATCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3195 GTATCC GTATCC(6/5)
BglII 3032 AGATCT A^GATCT
BsaHI 3767 GGCGCC G[GA]^CG[CT]C
BseAI 1061 TCCGGA T^CCGGA
BsiEI 268 CGGCCG CG[GA][CT]^CG
BsoBI 2625 CCCGGG C^[CT]CG[GA]G
BspEI 1061 TCCGGA T^CCGGA
BsrDI 2932 CATTGC GCAATG(2/0)
BssSI 11 CTCGTG CACGAG(-5/-1)
BstEII 1107 GGTAACC G^GT[ACGT]ACC
BstPI 1107 GGTAACC G^GT[ACGT]ACC
DraI 1228 TTTAAA TTT^AAA
EagI 268 CGGCCG C^GGCCG
Eam1105I 2391 GACCGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 268 CGGCCG C^GGCCG
Eco52I 268 CGGCCG C^GGCCG
EcoO65I 1107 GGTAACC G^GT[ACGT]ACC
EcoRI 3931 GAATTC G^AATTC
EcoRV 1554 GATATC GAT^ATC
EcoT22I 2067 ATGCAT ATGCA^T
FseI 269 GGCCGGCC GGCCGG^CC
HgaI 3120 GACGC GACGC(5/10)
Hin1I 3767 GGCGCC G[GA]^CG[CT]C
KasI 3767 GGCGCC G^GCGCC
MfeI 1457 CAATTG C^AATTG
MroI 1061 TCCGGA T^CCGGA
MunI 1457 CAATTG C^AATTG
NarI 3767 GGCGCC GG^CGCC
NsiI 2067 ATGCAT ATGCA^T
NspI 3489 ACATGC [GA]CATG^[CT]
PflMI 3824 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 1227 GTTTAAAC GTTT^AAAC
PshBI 3144 ATTAAT AT^TAAT
Psp1406I 2855 AACGTT AA^CGTT
SbfI 3917 CCTGCAGG CCTGCA^GG
SfoI 3767 GGCGCC GGC^GCC
SmaI 2625 CCCGGG CCC^GGG
Sse8387I 3917 CCTGCAGG CCTGCA^GG
Van91I 3824 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3214 TCTAGA T^CTAGA
XmaCI 2625 CCCGGG C^CCGGG
XmaI 2625 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 1718 GGTACC G^GTACC
3258 GGTACC
AccI 3002 GTAGAC GT^[AC][GT]AC
3362 GTCTAC
AccII 497 CGCG CG^CG
2698 CGCG
AcsI 1965 GAATTT [GA]^AATT[CT]
3931 GAATTC
AlwI 2089 GATCC GGATC(4/5)
2088 GGATC
AocI 1544 CCTGAGG CC^T[ACGT]AGG
2604 CCTCAGG
ApoI 1965 GAATTT [GA]^AATT[CT]
3931 GAATTC
Asp718I 1718 GGTACC G^GTACC
3258 GGTACC
AvrII 2207 CCTAGG C^CTAGG
3167 CCTAGG
BbrPI 594 CACGTG CAC^GTG
2582 CACGTG
BbsI 1603 GTCTTC GAAGAC(2/6)
4085 GTCTTC
BbvCI 969 CCTCAGC CCTCAGC(-5/-2)
2099 CCTCAGC
BglI 290 GCCTGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
294 GCCGGGCCGGC
BlnI 2207 CCTAGG C^CTAGG
3167 CCTAGG
BpuAI 1603 GTCTTC GAAGAC(2/6)
4085 GTCTTC
BsaAI 594 CACGTG [CT]AC^GT[GA]
2582 CACGTG
BsmI 125 GAATGC GAATGC(1/-1)
1648 GCATTC
BsrBI 1145 CCGCTC CCGCTC(-3/-3)
3924 GAGCGG
BstAPI 3487 GCACATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3906 GCAGGACTTGC
BstUI 497 CGCG CG^CG
2698 CGCG
BstYI 2088 GGATCC [GA]^GATC[CT]
3032 AGATCT
Bsu36I 1544 CCTGAGG CC^T[ACGT]AGG
2604 CCTCAGG
DrdI 4076 GACTCCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4369 GACTGCAGGGTC
Eco81I 1544 CCTGAGG CC^T[ACGT]AGG
2604 CCTCAGG
EcoNI 957 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3403 CCTGGGCCAGG
KpnI 1718 GGTACC GGTAC^C
3258 GGTACC
MflI 2088 GGATCC [GA]^GATC[CT]
3032 AGATCT
MvnI 497 CGCG CG^CG
2698 CGCG
NcoI 1112 CCATGG C^CATGG
3351 CCATGG
PmaCI 594 CACGTG CAC^GTG
2582 CACGTG
PmlI 594 CACGTG CAC^GTG
2582 CACGTG
SexAI 1501 ACCAGGT A^CC[AT]GGT
1721 ACCAGGT
XhoII 2088 GGATCC [GA]^GATC[CT]
3032 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AfaI AluI AlwNI Asp700I
AspHI AspI AvaII BalI BanI
BanII BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspMI BsrFI BsrGI BsrI BssKI
BstF5I BstNI BstXI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NaeI NciI NdeII NgoMIV NheI
NlaIII NlaIV PflFI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XcmI XmnI