##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AccI AccIII AclI AgeI AscI
AseI AsnI AviII BamHI BclI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BspHI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI EagI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KspI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfuI SnaBI SpeI
Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 1836 GGTACC G^GTACC
AcsI 3874 AAATTC [GA]^AATT[CT]
AflII 4324 CTTAAG C^TTAAG
Aor51HI 773 AGCGCT AGC^GCT
ApoI 3874 AAATTC [GA]^AATT[CT]
Asp700I 115 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1836 GGTACC G^GTACC
AspI 3198 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 818 CCTAGG C^CTAGG
BbvCI 2631 GCTGAGG CCTCAGC(-5/-2)
BcgI 780 GCAGGGGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4324 CTTAAG C^TTAAG
BlnI 818 CCTAGG C^CTAGG
BlpI 2845 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2845 GCTGAGC GC^T[ACGT]AGC
BsaBI 3543 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1365 GAGACG CGTCTC(1/5)
Bsp1407I 2696 TGTACA T^GTACA
BspLU11I 4500 ACATGT A^CATGT
BsrGI 2696 TGTACA T^GTACA
BssHII 857 GCGCGC G^CGCGC
BstXI 2681 CCACAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2845 GCTGAGC GC^T[ACGT]AGC
DraI 2457 TTTAAA TTT^AAA
DraIII 3097 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3106 GACAAGCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3020 CCACGG C^C[GA][CT]GG
Eco47III 773 AGCGCT AGC^GCT
KpnI 1836 GGTACC GGTAC^C
MamI 3543 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI 3198 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1287 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 1181 ACCAGGT A^CC[AT]GGT
SfiI 3144 GGCCAGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1539 CACCGGCG C[GA]^CCGG[CT]G
SmaI 472 CCCGGG CCC^GGG
SspBI 2696 TGTACA T^GTACA
SspI 4355 AATATT AAT^ATT
Tth111I 3198 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 472 CCCGGG C^CCGGG
XmaI 472 CCCGGG C^CCGGG
XmnI 115 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 4276 ACGTGT A^C[GA][CT]GT
4500 ACATGT
AhdI 1180 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3685 GACTGTGCGTC
Alw44I 1606 GTGCAC G^TGCAC
2268 GTGCAC
AocI 265 CCTCAGG CC^T[ACGT]AGG
2794 CCTGAGG
ApaI 1080 GGGCCC GGGCC^C
3528 GGGCCC
ApaLI 1606 GTGCAC G^TGCAC
2268 GTGCAC
AspEI 1180 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3685 GACTGTGCGTC
BalI 62 TGGCCA TGG^CCA
3152 TGGCCA
BciVI 52 GTATCC GTATCC(6/5)
2537 GTATCC
BglI 1985 GCCGCTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3145 GCCAGCGTGGC
BglII 2045 AGATCT A^GATCT
3806 AGATCT
BsaWI 2892 ACCGGA [AT]^CCGG[AT]
3562 ACCGGA
BsrBI 2974 CCGCTC CCGCTC(-3/-3)
3538 GAGCGG
Bsu36I 265 CCTCAGG CC^T[ACGT]AGG
2794 CCTGAGG
Eam1105I 1180 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3685 GACTGTGCGTC
Eco81I 265 CCTCAGG CC^T[ACGT]AGG
2794 CCTGAGG
HincII 1891 GTCAAC GT[CT]^[GA]AC
4075 GTTGAC
HindII 1891 GTCAAC GT[CT]^[GA]AC
4075 GTTGAC
MluNI 62 TGGCCA TGG^CCA
3152 TGGCCA
MscI 62 TGGCCA TGG^CCA
3152 TGGCCA
PaeR7I 3336 CTCGAG C^TCGAG
3480 CTCGAG
SphI 3264 GCATGC GCATG^C
4347 GCATGC
StuI 1897 AGGCCT AGG^CCT
2030 AGGCCT
XhoI 3336 CTCGAG C^TCGAG
3480 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI AlwI AlwNI AspHI AvaI
AvaII BanI BanII BbeI BbrPI
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII