##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbeI BbrPI BfrI BlnI
BsaAI BsaBI BseAI BsiEI BsiWI
Bsp1407I BspEI BspLU11I BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I FseI FspI
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NarI
NcoI NdeI NheI NotI NruI
NspI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 2846 GACGTC GACGT^C
AccI 2610 GTAGAC GT^[AC][GT]AC
AcyI 2846 GACGTC G[GA]^CG[CT]C
AflIII 2944 ACACGT A^C[GA][CT]GT
ApaI 4366 GGGCCC GGGCC^C
Asp700I 882 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 1999 CCTCAGC CCTCAGC(-5/-2)
BcgI 1520 CGAGGAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1651 AGATCT A^GATCT
BsaHI 2846 GACGTC G[GA]^CG[CT]C
BsaWI 4087 TCCGGT [AT]^CCGG[AT]
BsmI 4498 GAATGC GAATGC(1/-1)
BspDI 2880 ATCGAT AT^CGAT
BsrBI 3751 CCGCTC CCGCTC(-3/-3)
BsrFI 1619 GCCGGC [GA]^CCGG[CT]
BssHII 4310 GCGCGC G^CGCGC
BstXI 907 CCATTGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1619 GCCGGC [GA]^CCGG[CT]
ClaI 2880 ATCGAT AT^CGAT
EcoNI 1328 CCTTTTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 886 GAATTC G^AATTC
EcoRV 3731 GATATC GAT^ATC
EcoT22I 4584 ATGCAT ATGCA^T
Hin1I 2846 GACGTC G[GA]^CG[CT]C
HincII 2571 GTCAAC GT[CT]^[GA]AC
HindII 2571 GTCAAC GT[CT]^[GA]AC
HindIII 2370 AAGCTT A^AGCTT
NaeI 1619 GCCGGC GCC^GGC
NgoMIV 1619 GCCGGC G^CCGGC
NsiI 4584 ATGCAT ATGCA^T
PaeR7I 1518 CTCGAG C^TCGAG
PflMI 4106 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1544 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 567 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 53 GAGCTC GAGCT^C
SapI 2772 GAAGAGC GCTCTTC(1/4)
SbfI 3560 CCTGCAGG CCTGCA^GG
ScaI 3132 AGTACT AGT^ACT
SexAI 4108 ACCAGGT A^CC[AT]GGT
SfiI 2982 GGCCGAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 3560 CCTGCAGG CCTGCA^GG
Van91I 4106 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1518 CTCGAG C^TCGAG
XmnI 882 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 464 CGCG CG^CG
4311 CGCG
AocI 695 CCTGAGG CC^T[ACGT]AGG
2420 CCTGAGG
AspHI 53 GAGCTC G[AT]GC[AT]^C
678 GAGCAC
AspI 2543 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2722 GACGCAGTC
AvaI 1518 CTCGAG C^[CT]CG[GA]G
1860 CCCGAG
BamHI 468 GGATCC G^GATCC
4084 GGATCC
BanI 40 GGTGCC G^G[CT][GA]CC
480 GGCACC
BglI 2983 GCCGAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3360 GCCAGCTTGGC
BlpI 1760 GCTGAGC GC^T[ACGT]AGC
3843 GCTGAGC
Bpu1102I 1760 GCTGAGC GC^T[ACGT]AGC
3843 GCTGAGC
BsaI 1422 GAGACC GGTCTC(1/5)
1743 GAGACC
BsiHKAI 53 GAGCTC G[AT]GC[AT]^C
678 GAGCAC
BsoBI 1518 CTCGAG C^[CT]CG[GA]G
1860 CCCGAG
BspHI 2102 TCATGA T^CATGA
2185 TCATGA
BspMI 1439 ACCTGC ACCTGC(4/8)
1547 ACCTGC
BsrDI 2837 CATTGC GCAATG(2/0)
3353 CATTGC
BssSI 3026 CACGAG CACGAG(-5/-1)
3511 CACGAG
BstUI 464 CGCG CG^CG
4311 CGCG
Bsu36I 695 CCTGAGG CC^T[ACGT]AGG
2420 CCTGAGG
CelII 1760 GCTGAGC GC^T[ACGT]AGC
3843 GCTGAGC
DrdI 279 GACTTTTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3870 GACGGATATGTC
Eco81I 695 CCTGAGG CC^T[ACGT]AGG
2420 CCTGAGG
MvnI 464 CGCG CG^CG
4311 CGCG
PflFI 2543 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2722 GACGCAGTC
RcaI 2102 TCATGA T^CATGA
2185 TCATGA
SmlI 852 CTCAAG C^T[CT][GA]AG
1518 CTCGAG
Tth111I 2543 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2722 GACGCAGTC
XbaI 147 TCTAGA T^CTAGA
2936 TCTAGA
XcmI 3662 CCAGGGAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3808 CCAGTTCTGGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BalI BanII
BbsI BbvI BciVI BclI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII