##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
AocI Aor51HI AscI AspEI AspI
AviII BamHI BcgI BciVI BfrI
BsaBI BsiWI BspLU11I BsrBI BssHII
Bst1107I BstBI BstZ17I Bsu36I DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRV FseI FspI
KspI MamI MfeI MluI MunI
NotI NspV PacI PaeR7I PflFI
PinAI PmeI Psp1406I PvuI SacI
SacII SapI SbfI SfiI SfuI
SgrAI SmaI SphI Sse8387I SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 1515 GGTACC G^GTACC
AccIII 900 TCCGGA T^CCGGA
AflIII 2921 ACGTGT A^C[GA][CT]GT
ApaI 2638 GGGCCC GGGCC^C
AseI 3250 ATTAAT AT^TAAT
AsnI 3250 ATTAAT AT^TAAT
Asp718I 1515 GGTACC G^GTACC
AvrII 4472 CCTAGG C^CTAGG
BalI 3886 TGGCCA TGG^CCA
BbsI 80 GTCTTC GAAGAC(2/6)
BbvCI 2271 GCTGAGG CCTCAGC(-5/-2)
BclI 2844 TGATCA T^GATCA
BlnI 4472 CCTAGG C^CTAGG
BlpI 4081 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4081 GCTGAGC GC^T[ACGT]AGC
BpuAI 80 GTCTTC GAAGAC(2/6)
BsaI 4061 GGTCTC GGTCTC(1/5)
BseAI 900 TCCGGA T^CCGGA
BsiEI 96 CGGTCG CG[GA][CT]^CG
Bsp1407I 562 TGTACA T^GTACA
BspDI 1383 ATCGAT AT^CGAT
BspEI 900 TCCGGA T^CCGGA
BsrDI 4666 CATTGC GCAATG(2/0)
BsrFI 137 GCCGGC [GA]^CCGG[CT]
BsrGI 562 TGTACA T^GTACA
BstEII 2986 GGTCACC G^GT[ACGT]ACC
BstPI 2986 GGTCACC G^GT[ACGT]ACC
CelII 4081 GCTGAGC GC^T[ACGT]AGC
Cfr10I 137 GCCGGC [GA]^CCGG[CT]
ClaI 1383 ATCGAT AT^CGAT
CpoI 721 CGGACCG CG^G[AT]CCG
EcoO65I 2986 GGTCACC G^GT[ACGT]ACC
EcoRI 342 GAATTC G^AATTC
FbaI 2844 TGATCA T^GATCA
HpaI 2366 GTTAAC GTT^AAC
KpnI 1515 GGTACC GGTAC^C
MluNI 3886 TGGCCA TGG^CCA
MroI 900 TCCGGA T^CCGGA
MscI 3886 TGGCCA TGG^CCA
NaeI 137 GCCGGC GCC^GGC
NcoI 3309 CCATGG C^CATGG
NgoMIV 137 GCCGGC G^CCGGC
NruI 176 TCGCGA TCG^CGA
NspI 2584 ACATGC [GA]CATG^[CT]
PflMI 3106 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 3250 ATTAAT AT^TAAT
RsrII 721 CGGACCG CG^G[AT]CCG
SalI 975 GTCGAC G^TCGAC
ScaI 841 AGTACT AGT^ACT
SexAI 2791 ACCTGGT A^CC[AT]GGT
SnaBI 406 TACGTA TAC^GTA
SspBI 562 TGTACA T^GTACA
SspI 4045 AATATT AAT^ATT
StuI 146 AGGCCT AGG^CCT
Van91I 3106 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3598 TCTAGA T^CTAGA
XcmI 3747 CCACACACACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccI 975 GTCGAC GT^[AC][GT]AC
3925 GTAGAC
AccII 177 CGCG CG^CG
245 CGCG
AcyI 35 GGCGCC G[GA]^CG[CT]C
208 GGCGCC
Alw44I 2917 GTGCAC G^TGCAC
4318 GTGCAC
ApaLI 2917 GTGCAC G^TGCAC
4318 GTGCAC
Asp700I 3563 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3874 GAAGAATTTC
AvaI 44 CTCGGG C^[CT]CG[GA]G
3164 CCCGAG
BbeI 35 GGCGCC GGCGC^C
208 GGCGCC
BbrPI 2920 CACGTG CAC^GTG
4321 CACGTG
BcnI 70 CCCGG CC^[GC]GG
107 CCCGG
BglI 200 GCCTGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4468 GCCTCCTAGGC
BglII 3894 AGATCT A^GATCT
3963 AGATCT
BsaHI 35 GGCGCC G[GA]^CG[CT]C
208 GGCGCC
BsaWI 94 TCCGGT [AT]^CCGG[AT]
900 TCCGGA
BsmI 240 GCATTC GAATGC(1/-1)
4195 GCATTC
BsoBI 44 CTCGGG C^[CT]CG[GA]G
3164 CCCGAG
BssSI 1182 CTCGTG CACGAG(-5/-1)
2914 CTCGTG
BstAPI 4072 GCATAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4646 GCACCAACTGC
BstUI 177 CGCG CG^CG
245 CGCG
BstXI 4422 CCAGGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4695 CCAACTTGTTGG
EaeI 425 CGGCCA [CT]^GGCC[GA]
3886 TGGCCA
EcoNI 1307 CCTTTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2732 CCTTTGGAAGG
EcoT22I 3467 ATGCAT ATGCA^T
4328 ATGCAT
Hin1I 35 GGCGCC G[GA]^CG[CT]C
208 GGCGCC
KasI 35 GGCGCC G^GCGCC
208 GGCGCC
MvnI 177 CGCG CG^CG
245 CGCG
NarI 35 GGCGCC GG^CGCC
208 GGCGCC
NciI 70 CCCGG CC^[GC]GG
107 CCCGG
NdeI 1417 CATATG CA^TATG
4331 CATATG
NheI 2470 GCTAGC G^CTAGC
3613 GCTAGC
NsiI 3467 ATGCAT ATGCA^T
4328 ATGCAT
PmaCI 2920 CACGTG CAC^GTG
4321 CACGTG
PmlI 2920 CACGTG CAC^GTG
4321 CACGTG
PshAI 17 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1670 GACGGCAGTC
SfoI 35 GGCGCC GGC^GCC
208 GGCGCC
SmlI 2039 CTTGAG C^T[CT][GA]AG
3967 CTCAAG
SpeI 1436 ACTAGT A^CTAGT
2750 ACTAGT
XmnI 3563 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3874 GAAGAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BanII BbvI BfaI BmyI BpmI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BspHI BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII