################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII AclI AflII AgeI AhdI AocI Aor51HI AscI AspEI AspI AviII BamHI BcgI BciVI BfrI BsaBI BsiWI BspLU11I BsrBI BssHII Bst1107I BstBI BstZ17I Bsu36I DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV FseI FspI KspI MamI MfeI MluI MunI NotI NspV PacI PaeR7I PflFI PinAI PmeI Psp1406I PvuI SacI SacII SapI SbfI SfiI SfuI SgrAI SmaI SphI Sse8387I SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 1515 GGTACC G^GTACC AccIII 900 TCCGGA T^CCGGA AflIII 2921 ACGTGT A^C[GA][CT]GT ApaI 2638 GGGCCC GGGCC^C AseI 3250 ATTAAT AT^TAAT AsnI 3250 ATTAAT AT^TAAT Asp718I 1515 GGTACC G^GTACC AvrII 4472 CCTAGG C^CTAGG BalI 3886 TGGCCA TGG^CCA BbsI 80 GTCTTC GAAGAC(2/6) BbvCI 2271 GCTGAGG CCTCAGC(-5/-2) BclI 2844 TGATCA T^GATCA BlnI 4472 CCTAGG C^CTAGG BlpI 4081 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4081 GCTGAGC GC^T[ACGT]AGC BpuAI 80 GTCTTC GAAGAC(2/6) BsaI 4061 GGTCTC GGTCTC(1/5) BseAI 900 TCCGGA T^CCGGA BsiEI 96 CGGTCG CG[GA][CT]^CG Bsp1407I 562 TGTACA T^GTACA BspDI 1383 ATCGAT AT^CGAT BspEI 900 TCCGGA T^CCGGA BsrDI 4666 CATTGC GCAATG(2/0) BsrFI 137 GCCGGC [GA]^CCGG[CT] BsrGI 562 TGTACA T^GTACA BstEII 2986 GGTCACC G^GT[ACGT]ACC BstPI 2986 GGTCACC G^GT[ACGT]ACC CelII 4081 GCTGAGC GC^T[ACGT]AGC Cfr10I 137 GCCGGC [GA]^CCGG[CT] ClaI 1383 ATCGAT AT^CGAT CpoI 721 CGGACCG CG^G[AT]CCG EcoO65I 2986 GGTCACC G^GT[ACGT]ACC EcoRI 342 GAATTC G^AATTC FbaI 2844 TGATCA T^GATCA HpaI 2366 GTTAAC GTT^AAC KpnI 1515 GGTACC GGTAC^C MluNI 3886 TGGCCA TGG^CCA MroI 900 TCCGGA T^CCGGA MscI 3886 TGGCCA TGG^CCA NaeI 137 GCCGGC GCC^GGC NcoI 3309 CCATGG C^CATGG NgoMIV 137 GCCGGC G^CCGGC NruI 176 TCGCGA TCG^CGA NspI 2584 ACATGC [GA]CATG^[CT] PflMI 3106 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 3250 ATTAAT AT^TAAT RsrII 721 CGGACCG CG^G[AT]CCG SalI 975 GTCGAC G^TCGAC ScaI 841 AGTACT AGT^ACT SexAI 2791 ACCTGGT A^CC[AT]GGT SnaBI 406 TACGTA TAC^GTA SspBI 562 TGTACA T^GTACA SspI 4045 AATATT AAT^ATT StuI 146 AGGCCT AGG^CCT Van91I 3106 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3598 TCTAGA T^CTAGA XcmI 3747 CCACACACACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AccI 975 GTCGAC GT^[AC][GT]AC 3925 GTAGAC AccII 177 CGCG CG^CG 245 CGCG AcyI 35 GGCGCC G[GA]^CG[CT]C 208 GGCGCC Alw44I 2917 GTGCAC G^TGCAC 4318 GTGCAC ApaLI 2917 GTGCAC G^TGCAC 4318 GTGCAC Asp700I 3563 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3874 GAAGAATTTC AvaI 44 CTCGGG C^[CT]CG[GA]G 3164 CCCGAG BbeI 35 GGCGCC GGCGC^C 208 GGCGCC BbrPI 2920 CACGTG CAC^GTG 4321 CACGTG BcnI 70 CCCGG CC^[GC]GG 107 CCCGG BglI 200 GCCTGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4468 GCCTCCTAGGC BglII 3894 AGATCT A^GATCT 3963 AGATCT BsaHI 35 GGCGCC G[GA]^CG[CT]C 208 GGCGCC BsaWI 94 TCCGGT [AT]^CCGG[AT] 900 TCCGGA BsmI 240 GCATTC GAATGC(1/-1) 4195 GCATTC BsoBI 44 CTCGGG C^[CT]CG[GA]G 3164 CCCGAG BssSI 1182 CTCGTG CACGAG(-5/-1) 2914 CTCGTG BstAPI 4072 GCATAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4646 GCACCAACTGC BstUI 177 CGCG CG^CG 245 CGCG BstXI 4422 CCAGGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4695 CCAACTTGTTGG EaeI 425 CGGCCA [CT]^GGCC[GA] 3886 TGGCCA EcoNI 1307 CCTTTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2732 CCTTTGGAAGG EcoT22I 3467 ATGCAT ATGCA^T 4328 ATGCAT Hin1I 35 GGCGCC G[GA]^CG[CT]C 208 GGCGCC KasI 35 GGCGCC G^GCGCC 208 GGCGCC MvnI 177 CGCG CG^CG 245 CGCG NarI 35 GGCGCC GG^CGCC 208 GGCGCC NciI 70 CCCGG CC^[GC]GG 107 CCCGG NdeI 1417 CATATG CA^TATG 4331 CATATG NheI 2470 GCTAGC G^CTAGC 3613 GCTAGC NsiI 3467 ATGCAT ATGCA^T 4328 ATGCAT PmaCI 2920 CACGTG CAC^GTG 4321 CACGTG PmlI 2920 CACGTG CAC^GTG 4321 CACGTG PshAI 17 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1670 GACGGCAGTC SfoI 35 GGCGCC GGC^GCC 208 GGCGCC SmlI 2039 CTTGAG C^T[CT][GA]AG 3967 CTCAAG SpeI 1436 ACTAGT A^CTAGT 2750 ACTAGT XmnI 3563 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3874 GAAGAATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbvI BfaI BmyI BpmI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BspHI BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII