##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI AhdI AocI Aor51HI ApaI
AscI AspEI AspI BbeI BbrPI
BbvCI BcgI BfrI BglI BlpI
Bpu1102I BsaHI BseAI BsiEI BsiWI
BspDI BspEI BspHI BsrBI BsrFI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
FauI FseI HgaI Hin1I HindIII
HpaI KasI MfeI MluI MroI
MunI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 708 GGTACC G^GTACC
AflIII 3129 ACATGT A^C[GA][CT]GT
Alw44I 467 GTGCAC G^TGCAC
AlwNI 1336 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 467 GTGCAC G^TGCAC
AseI 3190 ATTAAT AT^TAAT
AsnI 3190 ATTAAT AT^TAAT
Asp700I 1647 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 708 GGTACC G^GTACC
AviII 3185 TGCGCA TGC^GCA
AvrII 82 CCTAGG C^CTAGG
BalI 2320 TGGCCA TGG^CCA
BanI 708 GGTACC G^G[CT][GA]CC
BanII 492 GGGCTC G[GA]GC[CT]^C
BciVI 1570 GTATCC GTATCC(6/5)
BclI 626 TGATCA T^GATCA
BlnI 82 CCTAGG C^CTAGG
BsaAI 721 CACGTA [CT]AC^GT[GA]
BsaWI 775 ACCGGA [AT]^CCGG[AT]
BsgI 501 CTGCAC GTGCAG(16/14)
BsmBI 1017 CGTCTC CGTCTC(1/5)
BsmI 2441 GCATTC GAATGC(1/-1)
Bsp1407I 2459 TGTACA T^GTACA
BspLU11I 3129 ACATGT A^CATGT
BspMI 2848 ACCTGC ACCTGC(4/8)
BsrGI 2459 TGTACA T^GTACA
BssSI 2463 CACGAG CACGAG(-5/-1)
BstXI 338 CCAGGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 2320 TGGCCA [CT]^GGCC[GA]
EcoRI 2971 GAATTC G^AATTC
EcoT22I 1414 ATGCAT ATGCA^T
FbaI 626 TGATCA T^GATCA
FspI 3185 TGCGCA TGC^GCA
HaeII 1330 AGCGCC [GA]GCGC^[CT]
KpnI 708 GGTACC GGTAC^C
KspI 54 CCGCGG CCGC^GG
MluNI 2320 TGGCCA TGG^CCA
MscI 2320 TGGCCA TGG^CCA
NcoI 899 CCATGG C^CATGG
NdeI 247 CATATG CA^TATG
NsiI 1414 ATGCAT ATGCA^T
PpuMI 45 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 3190 ATTAAT AT^TAAT
PvuII 192 CAGCTG CAG^CTG
SacII 54 CCGCGG CCGC^GG
ScaI 3055 AGTACT AGT^ACT
SphI 1412 GCATGC GCATG^C
SspBI 2459 TGTACA T^GTACA
SspI 2929 AATATT AAT^ATT
StuI 1112 AGGCCT AGG^CCT
XbaI 2445 TCTAGA T^CTAGA
XmnI 1647 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 2505 GTAGAC GT^[AC][GT]AC
3177 GTCTAC
AvaI 159 CCCGAG C^[CT]CG[GA]G
1150 CCCGAG
BamHI 1669 GGATCC G^GATCC
1838 GGATCC
BglII 683 AGATCT A^GATCT
2108 AGATCT
BsaBI 1658 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3027 GATATCTATC
BsaI 1153 GAGACC GGTCTC(1/5)
2161 GGTCTC
BsoBI 159 CCCGAG C^[CT]CG[GA]G
1150 CCCGAG
BssHII 449 GCGCGC G^CGCGC
451 GCGCGC
DraI 3003 TTTAAA TTT^AAA
3081 TTTAAA
DraII 45 GGGTCCC [GA]G^G[ACGT]CC[CT]
2827 AGGCCCC
DraIII 791 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1075 CACCAAGTG
DsaI 54 CCGCGG C^C[GA][CT]GG
899 CCATGG
EcoO109I 45 GGGTCCC [GA]G^G[ACGT]CC[CT]
2827 AGGCCCC
EcoRV 2915 GATATC GAT^ATC
3027 GATATC
MamI 1658 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3027 GATATCTATC
NspI 1412 GCATGC [GA]CATG^[CT]
3129 ACATGT
PflMI 122 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2177 CCAAAGTTTGG
PleI 2584 GACTC GAGTC(4/5)
342 GAGTC
SexAI 253 ACCTGGT A^CC[AT]GGT
1156 ACCTGGT
Van91I 122 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2177 CCAAAGTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII