##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccI AccIII AgeI
AocI ApaI AscI Asp718I AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BclI BglI BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BseRI
BsiWI BsmI BspEI BspLU11I BssSI
Bst1107I BstZ17I Bsu36I CelII DraI
Eco81I EcoNI EcoRV FbaI FseI
FspI HindIII HpaI KasI KpnI
MamI MluI MroI NarI NcoI
NheI NruI PacI PflFI PinAI
PmaCI PmeI PmlI PshAI SacI
SalI SbfI SexAI SfiI SfoI
SgrAI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I XbaI XcmI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AclI 1279 AACGTT AA^CGTT
AcyI 2083 GGCGTC G[GA]^CG[CT]C
AflII 1093 CTTAAG C^TTAAG
AflIII 1234 ACGTGT A^C[GA][CT]GT
AhdI 2078 GACTTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1610 AGCGCT AGC^GCT
AseI 3360 ATTAAT AT^TAAT
AsnI 3360 ATTAAT AT^TAAT
Asp700I 1588 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2078 GACTTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 855 GCAGAACGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 663 GGATAC GTATCC(6/5)
BfrI 1093 CTTAAG C^TTAAG
BsaAI 1968 TACGTA [CT]AC^GT[GA]
BsaHI 2083 GGCGTC G[GA]^CG[CT]C
BspDI 3884 ATCGAT AT^CGAT
BspMI 240 ACCTGC ACCTGC(4/8)
BssHII 614 GCGCGC G^CGCGC
BstBI 979 TTCGAA TT^CGAA
BstEII 2636 GGTCACC G^GT[ACGT]ACC
BstPI 2636 GGTCACC G^GT[ACGT]ACC
ClaI 3884 ATCGAT AT^CGAT
CpoI 327 CGGACCG CG^G[AT]CCG
DraIII 1230 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2078 GACTTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1610 AGCGCT AGC^GCT
EcoO65I 2636 GGTCACC G^GT[ACGT]ACC
EcoRI 913 GAATTC G^AATTC
EcoT14I 562 CCTTGG C^C[AT][AT]GG
EcoT22I 2427 ATGCAT ATGCA^T
HaeII 1610 AGCGCT [GA]GCGC^[CT]
Hin1I 2083 GGCGTC G[GA]^CG[CT]C
NotI 384 GCGGCCGC GC^GGCCGC
NsiI 2427 ATGCAT ATGCA^T
NspI 2237 ACATGC [GA]CATG^[CT]
NspV 979 TTCGAA TT^CGAA
PaeR7I 1159 CTCGAG C^TCGAG
PshBI 3360 ATTAAT AT^TAAT
Psp1406I 1279 AACGTT AA^CGTT
RsrII 327 CGGACCG CG^G[AT]CCG
SfuI 979 TTCGAA TT^CGAA
SnaBI 1968 TACGTA TAC^GTA
StyI 562 CCTTGG C^C[AT][AT]GG
XhoI 1159 CTCGAG C^TCGAG
XmnI 1588 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 913 GAATTC [GA]^AATT[CT]
3728 AAATTT
Alw44I 2935 GTGCAC G^TGCAC
3504 GTGCAC
ApaLI 2935 GTGCAC G^TGCAC
3504 GTGCAC
ApoI 913 GAATTC [GA]^AATT[CT]
3728 AAATTT
BalI 1274 TGGCCA TGG^CCA
2147 TGGCCA
BbsI 789 GAAGAC GAAGAC(2/6)
2207 GTCTTC
BpuAI 789 GAAGAC GAAGAC(2/6)
2207 GTCTTC
Bsp1407I 3892 TGTACA T^GTACA
3920 TGTACA
BspHI 1541 TCATGA T^CATGA
2046 TCATGA
BsrBI 2328 CCGCTC CCGCTC(-3/-3)
515 GAGCGG
BsrGI 3892 TGTACA T^GTACA
3920 TGTACA
BstAPI 16 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1913 GCAGAGGCTGC
DrdI 672 GACTTTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1008 GACGCACCCGTC
EagI 385 CGGCCG C^GGCCG
518 CGGCCG
EclXI 385 CGGCCG C^GGCCG
518 CGGCCG
Eco52I 385 CGGCCG C^GGCCG
518 CGGCCG
HincII 1203 GTTGAC GT[CT]^[GA]AC
3667 GTCAAC
HindII 1203 GTTGAC GT[CT]^[GA]AC
3667 GTCAAC
KspI 379 CCGCGG CCGC^GG
571 CCGCGG
MfeI 1178 CAATTG C^AATTG
2846 CAATTG
MluNI 1274 TGGCCA TGG^CCA
2147 TGGCCA
MscI 1274 TGGCCA TGG^CCA
2147 TGGCCA
MunI 1178 CAATTG C^AATTG
2846 CAATTG
NaeI 25 GCCGGC GCC^GGC
3156 GCCGGC
NdeI 1991 CATATG CA^TATG
2016 CATATG
NgoMIV 25 GCCGGC G^CCGGC
3156 GCCGGC
RcaI 1541 TCATGA T^CATGA
2046 TCATGA
SacII 379 CCGCGG CCGC^GG
571 CCGCGG
SapI 1726 GAAGAGC GCTCTTC(1/4)
3006 GCTCTTC
SspBI 3892 TGTACA T^GTACA
3920 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BglII
BmyI BpmI BsaI BsaJI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmaI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmaCI XmaI