##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AhdI Aor51HI ApaI AscI
Asp718I AspEI AviII AvrII BamHI
BanI BbeI BbrPI BcgI BfrI
BglI BlnI BsaHI BsaWI BseAI
BspDI BspEI BssHII BssSI BstEII
BstPI ClaI CpoI Eam1105I Eco47III
EcoNI EcoO65I EcoRI EcoRV FseI
FspI HaeII Hin1I HpaI KasI
KpnI KspI MfeI MluI MroI
MunI NarI NheI NotI NruI
PacI PflMI PinAI PmaCI PmlI
PshAI PvuI RsrII SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SpeI SphI Sse8387I Van91I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AclI 1463 AACGTT AA^CGTT
Alw44I 280 GTGCAC G^TGCAC
ApaLI 280 GTGCAC G^TGCAC
Asp700I 3303 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 4908 CTCGAG C^[CT]CG[GA]G
BbvCI 4336 CCTCAGC CCTCAGC(-5/-2)
BsiEI 4317 CGGCCG CG[GA][CT]^CG
BsiWI 4587 CGTACG C^GTACG
BsoBI 4908 CTCGAG C^[CT]CG[GA]G
BspLU11I 2347 ACATGT A^CATGT
BspMI 1848 ACCTGC ACCTGC(4/8)
BsrFI 4315 GCCGGC [GA]^CCGG[CT]
BstBI 518 TTCGAA TT^CGAA
Cfr10I 4315 GCCGGC [GA]^CCGG[CT]
DraIII 624 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 935 GACCCTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4317 CGGCCG C^GGCCG
EclXI 4317 CGGCCG C^GGCCG
Eco52I 4317 CGGCCG C^GGCCG
EcoT22I 513 ATGCAT ATGCA^T
HgaI 382 GACGC GACGC(5/10)
NaeI 4315 GCCGGC GCC^GGC
NgoMIV 4315 GCCGGC G^CCGGC
NsiI 513 ATGCAT ATGCA^T
NspV 518 TTCGAA TT^CGAA
PaeR7I 4908 CTCGAG C^TCGAG
PmeI 3663 GTTTAAAC GTTT^AAAC
Psp1406I 1463 AACGTT AA^CGTT
ScaI 5383 AGTACT AGT^ACT
SexAI 2721 ACCAGGT A^CC[AT]GGT
SfuI 518 TTCGAA TT^CGAA
SnaBI 4585 TACGTA TAC^GTA
StuI 50 AGGCCT AGG^CCT
SwaI 760 ATTTAAAT ATTT^AAAT
XbaI 3248 TCTAGA T^CTAGA
XcmI 4974 CCAATTACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4908 CTCGAG C^TCGAG
XmnI 3303 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AflIII 2347 ACATGT A^C[GA][CT]GT
2814 ACACGT
AocI 46 CCTCAGG CC^T[ACGT]AGG
815 CCTCAGG
AseI 3488 ATTAAT AT^TAAT
5531 ATTAAT
AsnI 3488 ATTAAT AT^TAAT
5531 ATTAAT
BalI 326 TGGCCA TGG^CCA
1044 TGGCCA
BciVI 4439 GGATAC GTATCC(6/5)
1087 GTATCC
BclI 355 TGATCA T^GATCA
4258 TGATCA
BcnI 1066 CCGGG CC^[GC]GG
1182 CCGGG
BglII 1497 AGATCT A^GATCT
3245 AGATCT
BsaAI 4585 TACGTA [CT]AC^GT[GA]
5065 CACGTA
BsaBI 356 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1303 GATGCTTATC
BsmBI 2817 CGTCTC CGTCTC(1/5)
4488 CGTCTC
BsmFI 3744 GGGAC GGGAC(10/14)
4379 GTCCC
Bsp1407I 4852 TGTACA T^GTACA
5250 TGTACA
BspHI 358 TCATGA T^CATGA
2407 TCATGA
BsrGI 4852 TGTACA T^GTACA
5250 TGTACA
Bst1107I 2378 GTATAC GTA^TAC
3444 GTATAC
BstXI 950 CCAGCTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1941 CCAAACCTCTGG
BstYI 1497 AGATCT [GA]^GATC[CT]
3245 AGATCT
BstZ17I 2378 GTATAC GTA^TAC
3444 GTATAC
Bsu36I 46 CCTCAGG CC^T[ACGT]AGG
815 CCTCAGG
Eco81I 46 CCTCAGG CC^T[ACGT]AGG
815 CCTCAGG
FbaI 355 TGATCA T^GATCA
4258 TGATCA
HindIII 390 AAGCTT A^AGCTT
1474 AAGCTT
MamI 356 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1303 GATGCTTATC
MflI 1497 AGATCT [GA]^GATC[CT]
3245 AGATCT
MluNI 326 TGGCCA TGG^CCA
1044 TGGCCA
MscI 326 TGGCCA TGG^CCA
1044 TGGCCA
MslI 1007 CATGTAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3105 CACTGCAGTG
NciI 1066 CCGGG CC^[GC]GG
1182 CCGGG
NcoI 9 CCATGG C^CATGG
4743 CCATGG
NdeI 2673 CATATG CA^TATG
4220 CATATG
PshBI 3488 ATTAAT AT^TAAT
5531 ATTAAT
RcaI 358 TCATGA T^CATGA
2407 TCATGA
SapI 1212 GCTCTTC GCTCTTC(1/4)
2885 GCTCTTC
SmlI 3701 CTCAAG C^T[CT][GA]AG
4908 CTCGAG
SspBI 4852 TGTACA T^GTACA
5250 TGTACA
SspI 4787 AATATT AAT^ATT
5522 AATATT
XhoII 1497 AGATCT [GA]^GATC[CT]
3245 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AspHI
AspI AvaII BanII BbsI BbvI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmI
Bsp1286I BsrBI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI PflFI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I