################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AatII AccIII AclI AflIII AgeI AhdI AscI AseI AsnI Asp700I AspEI BciVI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrGI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoO65I EcoT22I FseI HpaI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RsrII SalI ScaI SfuI SgrAI SnaBI SspBI SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 411 GGTACC G^GTACC AccI 3269 GTCTAC GT^[AC][GT]AC Alw44I 1059 GTGCAC G^TGCAC Aor51HI 2161 AGCGCT AGC^GCT ApaI 2352 GGGCCC GGGCC^C ApaLI 1059 GTGCAC G^TGCAC Asp718I 411 GGTACC G^GTACC AspI 74 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1681 TGCGCA TGC^GCA AvrII 24 CCTAGG C^CTAGG BamHI 2556 GGATCC G^GATCC BbeI 1367 GGCGCC GGCGC^C BbrPI 3345 CACGTG CAC^GTG BbvCI 334 CCTCAGC CCTCAGC(-5/-2) BclI 1102 TGATCA T^GATCA BglII 510 AGATCT A^GATCT BlnI 24 CCTAGG C^CTAGG BsaBI 3432 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 414 ACCGGA [AT]^CCGG[AT] BspHI 268 TCATGA T^CATGA BspMI 3710 GCAGGT ACCTGC(4/8) BsrDI 3913 CATTGC GCAATG(2/0) BssHII 6 GCGCGC G^CGCGC DraIII 473 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 2161 AGCGCT AGC^GCT EcoRI 2683 GAATTC G^AATTC EcoRV 4137 GATATC GAT^ATC FbaI 1102 TGATCA T^GATCA FspI 1681 TGCGCA TGC^GCA HindIII 653 AAGCTT A^AGCTT KasI 1367 GGCGCC G^GCGCC KpnI 411 GGTACC GGTAC^C KspI 929 CCGCGG CCGC^GG MamI 3432 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 1367 GGCGCC GG^CGCC PaeR7I 3465 CTCGAG C^TCGAG PflFI 74 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3345 CACGTG CAC^GTG PmlI 3345 CACGTG CAC^GTG PshAI 4236 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 268 TCATGA T^CATGA SacII 929 CCGCGG CCGC^GG SapI 1613 GAAGAGC GCTCTTC(1/4) SexAI 474 ACCTGGT A^CC[AT]GGT SfiI 2824 GGCCAGCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1367 GGCGCC GGC^GCC SmaI 2280 CCCGGG CCC^GGG SpeI 3898 ACTAGT A^CTAGT Tth111I 74 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 3465 CTCGAG C^TCGAG XmaCI 2280 CCCGGG C^CCGGG XmaI 2280 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcyI 45 GACGCC G[GA]^CG[CT]C 1367 GGCGCC BalI 715 TGGCCA TGG^CCA 2956 TGGCCA BbsI 3064 GAAGAC GAAGAC(2/6) 709 GTCTTC BcgI 2920 CGAGAGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1918 GCAGGGGCTTCG BpuAI 3064 GAAGAC GAAGAC(2/6) 709 GTCTTC BsaAI 426 TACGTG [CT]AC^GT[GA] 3345 CACGTG BsaHI 45 GACGCC G[GA]^CG[CT]C 1367 GGCGCC BsaI 1181 GAGACC GGTCTC(1/5) 3777 GAGACC BsiEI 78 CGGTCG CG[GA][CT]^CG 2245 CGACCG DrdI 2192 GACACCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3613 GACTTAAAAGTC Hin1I 45 GACGCC G[GA]^CG[CT]C 1367 GGCGCC HincII 596 GTCAAC GT[CT]^[GA]AC 2915 GTCAAC HindII 596 GTCAAC GT[CT]^[GA]AC 2915 GTCAAC MluNI 715 TGGCCA TGG^CCA 2956 TGGCCA MscI 715 TGGCCA TGG^CCA 2956 TGGCCA NaeI 112 GCCGGC GCC^GGC 4263 GCCGGC NgoMIV 112 GCCGGC G^CCGGC 4263 GCCGGC SphI 238 GCATGC GCATG^C 4201 GCATGC TfiI 1003 GATTC G^A[AT]TC 3437 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccII AciI AcsI AfaI AflII AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BfrI BglI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SmlI Sse8387I StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII