##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccIII AclI AflIII AgeI
AhdI AscI AseI AsnI Asp700I
AspEI BciVI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco52I EcoO65I EcoT22I FseI
HpaI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshBI
Psp1406I PvuI RsrII SalI ScaI
SfuI SgrAI SnaBI SspBI SspI
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 411 GGTACC G^GTACC
AccI 3269 GTCTAC GT^[AC][GT]AC
Alw44I 1059 GTGCAC G^TGCAC
Aor51HI 2161 AGCGCT AGC^GCT
ApaI 2352 GGGCCC GGGCC^C
ApaLI 1059 GTGCAC G^TGCAC
Asp718I 411 GGTACC G^GTACC
AspI 74 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1681 TGCGCA TGC^GCA
AvrII 24 CCTAGG C^CTAGG
BamHI 2556 GGATCC G^GATCC
BbeI 1367 GGCGCC GGCGC^C
BbrPI 3345 CACGTG CAC^GTG
BbvCI 334 CCTCAGC CCTCAGC(-5/-2)
BclI 1102 TGATCA T^GATCA
BglII 510 AGATCT A^GATCT
BlnI 24 CCTAGG C^CTAGG
BsaBI 3432 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 414 ACCGGA [AT]^CCGG[AT]
BspHI 268 TCATGA T^CATGA
BspMI 3710 GCAGGT ACCTGC(4/8)
BsrDI 3913 CATTGC GCAATG(2/0)
BssHII 6 GCGCGC G^CGCGC
DraIII 473 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2161 AGCGCT AGC^GCT
EcoRI 2683 GAATTC G^AATTC
EcoRV 4137 GATATC GAT^ATC
FbaI 1102 TGATCA T^GATCA
FspI 1681 TGCGCA TGC^GCA
HindIII 653 AAGCTT A^AGCTT
KasI 1367 GGCGCC G^GCGCC
KpnI 411 GGTACC GGTAC^C
KspI 929 CCGCGG CCGC^GG
MamI 3432 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1367 GGCGCC GG^CGCC
PaeR7I 3465 CTCGAG C^TCGAG
PflFI 74 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3345 CACGTG CAC^GTG
PmlI 3345 CACGTG CAC^GTG
PshAI 4236 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 268 TCATGA T^CATGA
SacII 929 CCGCGG CCGC^GG
SapI 1613 GAAGAGC GCTCTTC(1/4)
SexAI 474 ACCTGGT A^CC[AT]GGT
SfiI 2824 GGCCAGCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1367 GGCGCC GGC^GCC
SmaI 2280 CCCGGG CCC^GGG
SpeI 3898 ACTAGT A^CTAGT
Tth111I 74 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3465 CTCGAG C^TCGAG
XmaCI 2280 CCCGGG C^CCGGG
XmaI 2280 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 45 GACGCC G[GA]^CG[CT]C
1367 GGCGCC
BalI 715 TGGCCA TGG^CCA
2956 TGGCCA
BbsI 3064 GAAGAC GAAGAC(2/6)
709 GTCTTC
BcgI 2920 CGAGAGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1918 GCAGGGGCTTCG
BpuAI 3064 GAAGAC GAAGAC(2/6)
709 GTCTTC
BsaAI 426 TACGTG [CT]AC^GT[GA]
3345 CACGTG
BsaHI 45 GACGCC G[GA]^CG[CT]C
1367 GGCGCC
BsaI 1181 GAGACC GGTCTC(1/5)
3777 GAGACC
BsiEI 78 CGGTCG CG[GA][CT]^CG
2245 CGACCG
DrdI 2192 GACACCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3613 GACTTAAAAGTC
Hin1I 45 GACGCC G[GA]^CG[CT]C
1367 GGCGCC
HincII 596 GTCAAC GT[CT]^[GA]AC
2915 GTCAAC
HindII 596 GTCAAC GT[CT]^[GA]AC
2915 GTCAAC
MluNI 715 TGGCCA TGG^CCA
2956 TGGCCA
MscI 715 TGGCCA TGG^CCA
2956 TGGCCA
NaeI 112 GCCGGC GCC^GGC
4263 GCCGGC
NgoMIV 112 GCCGGC G^CCGGC
4263 GCCGGC
SphI 238 GCATGC GCATG^C
4201 GCATGC
TfiI 1003 GATTC G^A[AT]TC
3437 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BfaI BfrI BglI
BlpI BmyI BpmI Bpu1102I BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrBI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmlI Sse8387I StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII