################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbsI BbvCI BbvI BcgI BlnI BpuAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstUI BstZ17I Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I EcoNI EcoRV FauI Fnu4HI FseI FspI HgaI Hin1I ItaI KasI KpnI KspI MamI MluI MluNI MroI MscI MspA1I MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SpeI Sse8387I SwaI TseI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AlwNI 733 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 846 CCTCAGG CC^T[ACGT]AGG Aor51HI 507 AGCGCT AGC^GCT AspHI 1178 GAGCAC G[AT]GC[AT]^C AspI 2317 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 1593 CACGTG CAC^GTG BciVI 4531 GTATCC GTATCC(6/5) BcnI 3541 CCCGG CC^[GC]GG BglI 2408 GCCTAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 1178 GAGCAC G[AGT]GC[ACT]^C BseRI 1898 CTCCTC GAGGAG(10/8) BsgI 168 CTGCAC GTGCAG(16/14) BsiHKAI 1178 GAGCAC G[AT]GC[AT]^C BsmI 3069 GCATTC GAATGC(1/-1) Bsp1286I 1178 GAGCAC G[AGT]GC[ACT]^C BspLU11I 1570 ACATGT A^CATGT BstAPI 4112 GCATACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 111 TTCGAA TT^CGAA BstEII 3132 GGTAACC G^GT[ACGT]ACC BstPI 3132 GGTAACC G^GT[ACGT]ACC Bsu36I 846 CCTCAGG CC^T[ACGT]AGG CfoI 508 GCGC GCG^C EarI 676 CTCTTC CTCTTC(1/4) Eco47III 507 AGCGCT AGC^GCT Eco81I 846 CCTCAGG CC^T[ACGT]AGG EcoO65I 3132 GGTAACC G^GT[ACGT]ACC EcoT22I 810 ATGCAT ATGCA^T HaeII 507 AGCGCT [GA]GCGC^[CT] HhaI 508 GCGC GCG^C HinP1I 508 GCGC G^CGC HpaI 4308 GTTAAC GTT^AAC Ksp632I 676 CTCTTC CTCTTC(1/4) MfeI 2137 CAATTG C^AATTG MunI 2137 CAATTG C^AATTG NciI 3541 CCCGG CC^[GC]GG NlaIV 2006 GGTCCC GG[ACGT]^[ACGT]CC NsiI 810 ATGCAT ATGCA^T NspV 111 TTCGAA TT^CGAA PflFI 2317 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 103 GACTC GAGTC(4/5) PmaCI 1593 CACGTG CAC^GTG PmlI 1593 CACGTG CAC^GTG PpuMI 3941 AGGTCCT [GA]G^G[AT]CC[CT] ScaI 1155 AGTACT AGT^ACT SfuI 111 TTCGAA TT^CGAA SnaBI 4417 TACGTA TAC^GTA SphI 812 GCATGC GCATG^C TaqI 112 TCGA T^CGA Tth111I 2317 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 112 TCGA T^CGA XbaI 2025 TCTAGA T^CTAGA XcmI 2224 CCATTCTCTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflII 98 CTTAAG C^TTAAG 3717 CTTAAG BfrI 98 CTTAAG C^TTAAG 3717 CTTAAG BglII 2838 AGATCT A^GATCT 3555 AGATCT BlpI 8 GCTCAGC GC^T[ACGT]AGC 596 GCTCAGC Bpu1102I 8 GCTCAGC GC^T[ACGT]AGC 596 GCTCAGC BsaWI 54 TCCGGT [AT]^CCGG[AT] 458 ACCGGA BsmFI 1857 GTCCC GGGAC(10/14) 2007 GTCCC BspMI 4424 ACCTGC ACCTGC(4/8) 3399 GCAGGT BstNI 2074 CCTGG CC^[AT]GG 3202 CCAGG BstXI 817 CCAAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3574 CCAGAGTGCTGG CelII 8 GCTCAGC GC^T[ACGT]AGC 596 GCTCAGC DraII 551 AGGCCCT [GA]G^G[ACGT]CC[CT] 3941 AGGTCCT EcoO109I 551 AGGCCCT [GA]G^G[ACGT]CC[CT] 3941 AGGTCCT EcoRII 2074 CCTGG ^CC[AT]GG 3202 CCAGG HincII 4251 GTTGAC GT[CT]^[GA]AC 4308 GTTAAC HindII 4251 GTTGAC GT[CT]^[GA]AC 4308 GTTAAC HindIII 1167 AAGCTT A^AGCTT 3305 AAGCTT MvaI 2074 CCTGG CC^[AT]GG 3202 CCAGG SfcI 2329 CTGTAG C^T[GA][CT]AG 3419 CTATAG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AcsI AfaI AflIII AluI AlwI ApoI Asp700I AvaII BclI BfaI BpmI BsaAI BsaI BsaJI BsiYI BslI BsmAI Bsp1407I BspHI BsrGI BsrI BssKI BstF5I BstYI Cac8I Cfr13I DdeI DpnII DraI EcoRI EcoT14I FbaI FokI HaeIII HapII HinfI HpaII HphI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MwoI NdeI NdeII NlaIII NspI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI SspBI SspI StuI StyI TfiI Tru9I Tsp45I Tsp509I TspRI XhoII XmnI