##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbsI BbvCI BbvI BcgI
BlnI BpuAI BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstUI BstZ17I Cfr10I
ClaI CpoI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco52I
EcoNI EcoRV FauI Fnu4HI FseI
FspI HgaI Hin1I ItaI KasI
KpnI KspI MamI MluI MluNI
MroI MscI MspA1I MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI PacI PaeR7I PflMI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SpeI Sse8387I
SwaI TseI Van91I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AlwNI 733 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 846 CCTCAGG CC^T[ACGT]AGG
Aor51HI 507 AGCGCT AGC^GCT
AspHI 1178 GAGCAC G[AT]GC[AT]^C
AspI 2317 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1593 CACGTG CAC^GTG
BciVI 4531 GTATCC GTATCC(6/5)
BcnI 3541 CCCGG CC^[GC]GG
BglI 2408 GCCTAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 1178 GAGCAC G[AGT]GC[ACT]^C
BseRI 1898 CTCCTC GAGGAG(10/8)
BsgI 168 CTGCAC GTGCAG(16/14)
BsiHKAI 1178 GAGCAC G[AT]GC[AT]^C
BsmI 3069 GCATTC GAATGC(1/-1)
Bsp1286I 1178 GAGCAC G[AGT]GC[ACT]^C
BspLU11I 1570 ACATGT A^CATGT
BstAPI 4112 GCATACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 111 TTCGAA TT^CGAA
BstEII 3132 GGTAACC G^GT[ACGT]ACC
BstPI 3132 GGTAACC G^GT[ACGT]ACC
Bsu36I 846 CCTCAGG CC^T[ACGT]AGG
CfoI 508 GCGC GCG^C
EarI 676 CTCTTC CTCTTC(1/4)
Eco47III 507 AGCGCT AGC^GCT
Eco81I 846 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3132 GGTAACC G^GT[ACGT]ACC
EcoT22I 810 ATGCAT ATGCA^T
HaeII 507 AGCGCT [GA]GCGC^[CT]
HhaI 508 GCGC GCG^C
HinP1I 508 GCGC G^CGC
HpaI 4308 GTTAAC GTT^AAC
Ksp632I 676 CTCTTC CTCTTC(1/4)
MfeI 2137 CAATTG C^AATTG
MunI 2137 CAATTG C^AATTG
NciI 3541 CCCGG CC^[GC]GG
NlaIV 2006 GGTCCC GG[ACGT]^[ACGT]CC
NsiI 810 ATGCAT ATGCA^T
NspV 111 TTCGAA TT^CGAA
PflFI 2317 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 103 GACTC GAGTC(4/5)
PmaCI 1593 CACGTG CAC^GTG
PmlI 1593 CACGTG CAC^GTG
PpuMI 3941 AGGTCCT [GA]G^G[AT]CC[CT]
ScaI 1155 AGTACT AGT^ACT
SfuI 111 TTCGAA TT^CGAA
SnaBI 4417 TACGTA TAC^GTA
SphI 812 GCATGC GCATG^C
TaqI 112 TCGA T^CGA
Tth111I 2317 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 112 TCGA T^CGA
XbaI 2025 TCTAGA T^CTAGA
XcmI 2224 CCATTCTCTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflII 98 CTTAAG C^TTAAG
3717 CTTAAG
BfrI 98 CTTAAG C^TTAAG
3717 CTTAAG
BglII 2838 AGATCT A^GATCT
3555 AGATCT
BlpI 8 GCTCAGC GC^T[ACGT]AGC
596 GCTCAGC
Bpu1102I 8 GCTCAGC GC^T[ACGT]AGC
596 GCTCAGC
BsaWI 54 TCCGGT [AT]^CCGG[AT]
458 ACCGGA
BsmFI 1857 GTCCC GGGAC(10/14)
2007 GTCCC
BspMI 4424 ACCTGC ACCTGC(4/8)
3399 GCAGGT
BstNI 2074 CCTGG CC^[AT]GG
3202 CCAGG
BstXI 817 CCAAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3574 CCAGAGTGCTGG
CelII 8 GCTCAGC GC^T[ACGT]AGC
596 GCTCAGC
DraII 551 AGGCCCT [GA]G^G[ACGT]CC[CT]
3941 AGGTCCT
EcoO109I 551 AGGCCCT [GA]G^G[ACGT]CC[CT]
3941 AGGTCCT
EcoRII 2074 CCTGG ^CC[AT]GG
3202 CCAGG
HincII 4251 GTTGAC GT[CT]^[GA]AC
4308 GTTAAC
HindII 4251 GTTGAC GT[CT]^[GA]AC
4308 GTTAAC
HindIII 1167 AAGCTT A^AGCTT
3305 AAGCTT
MvaI 2074 CCTGG CC^[AT]GG
3202 CCAGG
SfcI 2329 CTGTAG C^T[GA][CT]AG
3419 CTATAG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI Asp700I AvaII BclI BfaI
BpmI BsaAI BsaI BsaJI BsiYI
BslI BsmAI Bsp1407I BspHI BsrGI
BsrI BssKI BstF5I BstYI Cac8I
Cfr13I DdeI DpnII DraI EcoRI
EcoT14I FbaI FokI HaeIII HapII
HinfI HpaII HphI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MwoI NdeI
NdeII NlaIII NspI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
SspBI SspI StuI StyI TfiI
Tru9I Tsp45I Tsp509I TspRI XhoII
XmnI