##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AflIII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
AspEI AspI AviII AvrII BbrPI
BcgI BclI BlnI BsaAI BsaWI
BseAI BsgI BsiWI BspDI BspEI
BspLU11I BsrFI BssSI BstEII BstPI
BstXI Cfr10I ClaI DraIII DrdI
Eam1105I Eco47III EcoO65I FbaI FseI
FspI HpaI MfeI MluI MroI
MunI NaeI NgoMIV NotI NruI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
SalI SexAI SfiI SgrAI SnaBI
SphI StuI Tth111I XbaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 723 GGTACC G^GTACC
AclI 2268 AACGTT AA^CGTT
AocI 416 CCTCAGG CC^T[ACGT]AGG
Asp700I 1752 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 723 GGTACC G^GTACC
BamHI 3115 GGATCC G^GATCC
BbvCI 837 CCTCAGC CCTCAGC(-5/-2)
BglI 105 GCCTCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2714 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2714 GCTCAGC GC^T[ACGT]AGC
BsiEI 124 CGGCCG CG[GA][CT]^CG
BsmBI 51 GAGACG CGTCTC(1/5)
BspMI 2227 ACCTGC ACCTGC(4/8)
BssHII 41 GCGCGC G^CGCGC
Bst1107I 3466 GTATAC GTA^TAC
BstAPI 1178 GCACTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1614 TTCGAA TT^CGAA
BstZ17I 3466 GTATAC GTA^TAC
Bsu36I 416 CCTCAGG CC^T[ACGT]AGG
CelII 2714 GCTCAGC GC^T[ACGT]AGC
CpoI 128 CGGACCG CG^G[AT]CCG
DraII 1892 AGGCCCC [GA]G^G[ACGT]CC[CT]
EagI 124 CGGCCG C^GGCCG
EclXI 124 CGGCCG C^GGCCG
Eco52I 124 CGGCCG C^GGCCG
Eco81I 416 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1892 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 1406 GATATC GAT^ATC
KpnI 723 GGTACC GGTAC^C
KspI 7 CCGCGG CCGC^GG
NcoI 264 CCATGG C^CATGG
NdeI 3497 CATATG CA^TATG
NheI 3230 GCTAGC G^CTAGC
NspV 1614 TTCGAA TT^CGAA
PacI 4438 TTAATTAA TTAAT^TAA
PflMI 1788 CCAGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 2268 AACGTT AA^CGTT
RsrII 128 CGGACCG CG^G[AT]CCG
SacII 7 CCGCGG CCGC^GG
SapI 1556 GAAGAGC GCTCTTC(1/4)
SbfI 2228 CCTGCAGG CCTGCA^GG
SfuI 1614 TTCGAA TT^CGAA
SmaI 69 CCCGGG CCC^GGG
SpeI 830 ACTAGT A^CTAGT
Sse8387I 2228 CCTGCAGG CCTGCA^GG
SspI 3690 AATATT AAT^ATT
SwaI 4281 ATTTAAAT ATTT^AAAT
Van91I 1788 CCAGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 69 CCCGGG C^CCGGG
XmaI 69 CCCGGG C^CCGGG
XmnI 1752 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 8 CGCG CG^CG
42 CGCG
AflII 320 CTTAAG C^TTAAG
3774 CTTAAG
Alw44I 1635 GTGCAC G^TGCAC
3490 GTGCAC
ApaLI 1635 GTGCAC G^TGCAC
3490 GTGCAC
BalI 2959 TGGCCA TGG^CCA
4186 TGGCCA
BbeI 64 GGCGCC GGCGC^C
161 GGCGCC
BciVI 622 GGATAC GTATCC(6/5)
2857 GGATAC
BfrI 320 CTTAAG C^TTAAG
3774 CTTAAG
BsaI 2147 GAGACC GGTCTC(1/5)
2052 GGTCTC
BsrBI 34 GAGCGG CCGCTC(-3/-3)
2828 GAGCGG
BstUI 8 CGCG CG^CG
42 CGCG
EcoNI 3558 CCTAGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4257 CCTGCAGAAGG
EcoT14I 264 CCATGG C^C[AT][AT]GG
1860 CCTTGG
EcoT22I 3163 ATGCAT ATGCA^T
3381 ATGCAT
HaeII 64 GGCGCC [GA]GCGC^[CT]
161 GGCGCC
KasI 64 GGCGCC G^GCGCC
161 GGCGCC
MaeII 2269 ACGT A^CGT
3053 ACGT
MluNI 2959 TGGCCA TGG^CCA
4186 TGGCCA
MscI 2959 TGGCCA TGG^CCA
4186 TGGCCA
MvnI 8 CGCG CG^CG
42 CGCG
NarI 64 GGCGCC GG^CGCC
161 GGCGCC
NsiI 3163 ATGCAT ATGCA^T
3381 ATGCAT
SacI 1775 GAGCTC GAGCT^C
2383 GAGCTC
ScaI 1301 AGTACT AGT^ACT
3369 AGTACT
SfoI 64 GGCGCC GGC^GCC
161 GGCGCC
StyI 264 CCATGG C^C[AT][AT]GG
1860 CCTTGG
TaqI 1615 TCGA T^CGA
2085 TCGA
TthHB8I 1615 TCGA T^CGA
2085 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaBI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspHI BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DsaI
EaeI EarI EcoRI EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII