##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
Aor51HI ApaI AscI AseI AsnI
Asp718I BalI BbeI BbvCI BcgI
BclI BglI BsaHI BsiWI BsmBI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
FauI FbaI FseI Hin1I KasI
KpnI KspI MfeI MluNI MscI
MunI NaeI NarI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 322 TCCGGA T^CCGGA
AflII 1787 CTTAAG C^TTAAG
AhdI 2193 GACAGTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 832 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2193 GACAGTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1680 CCCGGG C^[CT]CG[GA]G
AviII 819 TGCGCA TGC^GCA
AvrII 3941 CCTAGG C^CTAGG
BamHI 1473 GGATCC G^GATCC
BbrPI 3559 CACGTG CAC^GTG
BfrI 1787 CTTAAG C^TTAAG
BglII 3298 AGATCT A^GATCT
BlnI 3941 CCTAGG C^CTAGG
BsaWI 322 TCCGGA [AT]^CCGG[AT]
BseAI 322 TCCGGA T^CCGGA
BsiEI 2900 CGGTCG CG[GA][CT]^CG
BsoBI 1680 CCCGGG C^[CT]CG[GA]G
BspEI 322 TCCGGA T^CCGGA
BsrDI 1912 CATTGC GCAATG(2/0)
BssSI 3417 CTCGTG CACGAG(-5/-1)
BstAPI 1534 GCAGGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 1217 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 527 GACATGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 408 CGGCCA [CT]^GGCC[GA]
Eam1105I 2193 GACAGTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3936 CCTCCCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 143 GATATC GAT^ATC
EcoT22I 1909 ATGCAT ATGCA^T
FspI 819 TGCGCA TGC^GCA
MluI 4161 ACGCGT A^CGCGT
MroI 322 TCCGGA T^CCGGA
NcoI 966 CCATGG C^CATGG
NheI 89 GCTAGC G^CTAGC
NsiI 1909 ATGCAT ATGCA^T
PflMI 2028 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3559 CACGTG CAC^GTG
PmlI 3559 CACGTG CAC^GTG
SacI 947 GAGCTC GAGCT^C
SexAI 1218 ACCTGGT A^CC[AT]GGT
SmaI 1680 CCCGGG CCC^GGG
SspI 3503 AATATT AAT^ATT
StuI 1536 AGGCCT AGG^CCT
SwaI 2306 ATTTAAAT ATTT^AAAT
Van91I 2028 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1518 TCTAGA T^CTAGA
XmaCI 1680 CCCGGG C^CCGGG
XmaI 1680 CCCGGG C^CCGGG
XmnI 832 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 67 CGCG CG^CG
4162 CGCG
AflIII 4124 ACGTGT A^C[GA][CT]GT
4161 ACGCGT
Alw44I 2329 GTGCAC G^TGCAC
4084 GTGCAC
AlwNI 2731 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
3708 CAGGACCTG
AocI 2778 CCTGAGG CC^T[ACGT]AGG
3988 CCTTAGG
ApaLI 2329 GTGCAC G^TGCAC
4084 GTGCAC
AspI 2437 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2650 GACAATGTC
BanI 1222 GGTGCC G^G[CT][GA]CC
2817 GGTGCC
BanII 947 GAGCTC G[GA]GC[CT]^C
2774 GAGCCC
BciVI 581 GGATAC GTATCC(6/5)
1528 GGATAC
BsaBI 331 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1468 GATGTGGATC
BsaI 2259 GAGACC GGTCTC(1/5)
2284 GGTCTC
BsmI 601 GAATGC GAATGC(1/-1)
3683 GCATTC
BsrFI 480 ACCGGC [GA]^CCGG[CT]
2898 GCCGGT
BstUI 67 CGCG CG^CG
4162 CGCG
Bsu36I 2778 CCTGAGG CC^T[ACGT]AGG
3988 CCTTAGG
Cfr10I 480 ACCGGC [GA]^CCGG[CT]
2898 GCCGGT
DsaI 405 CCACGG C^C[GA][CT]GG
966 CCATGG
Eco81I 2778 CCTGAGG CC^T[ACGT]AGG
3988 CCTTAGG
HaeII 56 AGCGCC [GA]GCGC^[CT]
3667 AGCGCC
HgaI 1168 GACGC GACGC(5/10)
4160 GACGC
HincII 28 GTTAAC GT[CT]^[GA]AC
1264 GTTAAC
HindII 28 GTTAAC GT[CT]^[GA]AC
1264 GTTAAC
HindIII 1702 AAGCTT A^AGCTT
2148 AAGCTT
HpaI 28 GTTAAC GTT^AAC
1264 GTTAAC
MamI 331 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1468 GATGTGGATC
MspA1I 2707 CAGCTG C[AC]G^C[GT]G
3660 CAGCTG
MvnI 67 CGCG CG^CG
4162 CGCG
NspI 1722 GCATGT [GA]CATG^[CT]
3514 ACATGC
PflFI 2437 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2650 GACAATGTC
PpuMI 1676 AGGACCC [GA]G^G[AT]CC[CT]
3709 AGGACCT
PshAI 117 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
493 GACGGTGGTC
PstI 2761 CTGCAG CTGCA^G
3279 CTGCAG
PvuII 2707 CAGCTG CAG^CTG
3660 CAGCTG
SmlI 1787 CTTAAG C^T[CT][GA]AG
1930 CTTGAG
Tth111I 2437 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2650 GACAATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII