##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AocI Aor51HI AscI AseI AsnI
Asp718I AspI AvrII BamHI BbeI
BbrPI BcgI BlnI BsaHI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrDI BsrFI BsrGI BstBI BstEII
BstPI Bsu36I Cfr10I ClaI CpoI
DrdI Eco47III Eco81I EcoNI EcoO65I
EcoRV EcoT22I FseI Hin1I HpaI
KasI KpnI MluI MroI NaeI
NarI NgoMIV NheI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
RsrII SalI ScaI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
StuI SwaI Tth111I Van91I
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 3593 GTATAC GT^[AC][GT]AC
AflII 3975 CTTAAG C^TTAAG
AhdI 2393 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1746 GTGCAC G^TGCAC
ApaI 4185 GGGCCC GGGCC^C
ApaLI 1746 GTGCAC G^TGCAC
AspEI 2393 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2709 TGCGCA TGC^GCA
BanI 3302 GGTGCC G^G[CT][GA]CC
BbvCI 2458 GCTGAGG CCTCAGC(-5/-2)
BciVI 3316 GGATAC GTATCC(6/5)
BclI 3673 TGATCA T^GATCA
BfrI 3975 CTTAAG C^TTAAG
BlpI 1781 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1781 GCTGAGC GC^T[ACGT]AGC
BsaAI 3577 TACGTG [CT]AC^GT[GA]
BsaWI 2788 ACCGGA [AT]^CCGG[AT]
BssHII 352 GCGCGC G^CGCGC
BssSI 1333 CACGAG CACGAG(-5/-1)
Bst1107I 3593 GTATAC GTA^TAC
BstXI 1242 CCACCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3593 GTATAC GTA^TAC
CelII 1781 GCTGAGC GC^T[ACGT]AGC
DraIII 362 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2393 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1961 GAATTC G^AATTC
FbaI 3673 TGATCA T^GATCA
FspI 2709 TGCGCA TGC^GCA
KspI 243 CCGCGG CCGC^GG
MfeI 4788 CAATTG C^AATTG
MunI 4788 CAATTG C^AATTG
NdeI 3388 CATATG CA^TATG
NotI 6 GCGGCCGC GC^GGCCGC
PaeR7I 2013 CTCGAG C^TCGAG
PvuI 1018 CGATCG CGAT^CG
SacI 72 GAGCTC GAGCT^C
SacII 243 CCGCGG CCGC^GG
SbfI 2236 CCTGCAGG CCTGCA^GG
SfiI 2233 GGCCCTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 46 CCCGGG CCC^GGG
Sse8387I 2236 CCTGCAGG CCTGCA^GG
XbaI 538 TCTAGA T^CTAGA
XhoI 2013 CTCGAG C^TCGAG
XmaCI 46 CCCGGG C^CCGGG
XmaI 46 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AclI 3149 AACGTT AA^CGTT
4127 AACGTT
BalI 2146 TGGCCA TGG^CCA
3454 TGGCCA
BglI 2234 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4039 GCCATGTCGGC
BglII 738 AGATCT A^GATCT
4377 AGATCT
BsaBI 512 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2984 GATTTGGATC
BsaI 1308 GGTCTC GGTCTC(1/5)
2010 GGTCTC
BspLU11I 1947 ACATGT A^CATGT
3090 ACATGT
EagI 7 CGGCCG C^GGCCG
2498 CGGCCG
EclXI 7 CGGCCG C^GGCCG
2498 CGGCCG
Eco52I 7 CGGCCG C^GGCCG
2498 CGGCCG
HaeII 247 GGCGCT [GA]GCGC^[CT]
1487 GGCGCT
HindIII 346 AAGCTT A^AGCTT
1371 AAGCTT
MamI 512 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2984 GATTTGGATC
MluNI 2146 TGGCCA TGG^CCA
3454 TGGCCA
MscI 2146 TGGCCA TGG^CCA
3454 TGGCCA
NcoI 2623 CCATGG C^CATGG
2778 CCATGG
Psp1406I 3149 AACGTT AA^CGTT
4127 AACGTT
SapI 638 GAAGAGC GCTCTTC(1/4)
1473 GAAGAGC
SexAI 588 ACCAGGT A^CC[AT]GGT
4625 ACCTGGT
XcmI 2031 CCAACAGTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2051 CCATTTGCCAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BspMI
BsrBI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI